Structured Review

Pfizer Inc pbmc
Pbmc, supplied by Pfizer Inc, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pbmc/product/Pfizer Inc
Average 92 stars, based on 3 article reviews
Price from $9.99 to $1999.99
pbmc - by Bioz Stars, 2020-09
92/100 stars

Images

Related Articles

Positive Control:

Article Title: Synthesis of a Novel Thiazolidinedione and Evaluation of Its Modulatory Effect on IFN-γ, IL-6, IL-17A, and IL-22 Production in PBMCs from Rheumatoid Arthritis Patients
Article Snippet: .. Cells were stimulated with PMA (Sigma) + ionomycin (Sigma) for PBMCs or with Conocavalia-A (ConA) for splenocyte, in the presence or absence of TM-17 at concentrations of 1, 10, and 100 μ M. Methyprednisolone 100 μ M (Pfizer) was used as a positive control. .. Cells were incubated for 48 hours at 37°C in humidified 5% CO2 incubator.

Stable Transfection:

Article Title: Role of the Excitability Brake Potassium Current IKD in Cold Allodynia Induced by Chronic Peripheral Nerve Injury
Article Snippet: .. HEK-293 cells (CRL-1573, RRID: CVCL_0045) stably expressing mTRPM8 (HEK-293-TRPM8-myc cells) were used to evaluate the blocking effect of PBMC (Pfizer) on TRPM8 channels. .. HEK-293-TRPM8-myc cells were cultured in DMEM containing 10% of FBS and antibiotics.

Isolation:

Article Title: Phenotype and functionality of CD4+ and CD8+ T cells in the upper reproductive tract of healthy premenopausal women
Article Snippet: .. PBMC were isolated by Ficoll-Hypaque (Pfizer, New York, NY) density gradient centrifugation, and washed in phosphate-buffered saline (PBS). ..

Article Title: Magnitude and Complexity of Rectal Mucosa HIV-1-Specific CD8+ T-Cell Responses during Chronic Infection Reflect Clinical Status
Article Snippet: .. PBMC were isolated from blood using Ficoll-Paque™ (Pfizer-Pharmacia, New York, NY). .. Isolation of mononuclear cells from rectal biopsies was done using an optimized collagenase digestion procedure as previously described , .

Blocking Assay:

Article Title: Role of the Excitability Brake Potassium Current IKD in Cold Allodynia Induced by Chronic Peripheral Nerve Injury
Article Snippet: .. HEK-293 cells (CRL-1573, RRID: CVCL_0045) stably expressing mTRPM8 (HEK-293-TRPM8-myc cells) were used to evaluate the blocking effect of PBMC (Pfizer) on TRPM8 channels. .. HEK-293-TRPM8-myc cells were cultured in DMEM containing 10% of FBS and antibiotics.

Article Title: Dysfunction of CD19+CD24hiCD27+ B regulatory cells in patients with bullous pemphigoid
Article Snippet: .. To investigate the role of TNF-α in autoantibody production, patient-derived PBMCs were treated with etanercept (Pfizer, USA) to block the effect of TNF-α. .. Because the therapeutic range of residual serum concentration is between 1 and 10 μg/ml, we used 10 μg/ml of etanercept to incubate PBMCs for 72 hours.

Expressing:

Article Title: Role of the Excitability Brake Potassium Current IKD in Cold Allodynia Induced by Chronic Peripheral Nerve Injury
Article Snippet: .. HEK-293 cells (CRL-1573, RRID: CVCL_0045) stably expressing mTRPM8 (HEK-293-TRPM8-myc cells) were used to evaluate the blocking effect of PBMC (Pfizer) on TRPM8 channels. .. HEK-293-TRPM8-myc cells were cultured in DMEM containing 10% of FBS and antibiotics.

Gradient Centrifugation:

Article Title: Phenotype and functionality of CD4+ and CD8+ T cells in the upper reproductive tract of healthy premenopausal women
Article Snippet: .. PBMC were isolated by Ficoll-Hypaque (Pfizer, New York, NY) density gradient centrifugation, and washed in phosphate-buffered saline (PBS). ..

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 92
    Pfizer Inc pbmcs
    Modified function of Bregs were due to the expression of <t>TNF-α.</t> ( A ) Levels of TNF-α, IFN-γ, IL-10, IL-4, IL-22 and IL-23 mRNA in CD19 + CD24 hi CD27 + Bregs after sorting from <t>PBMCs</t> of 5 patients and 4 healthy controls. ( B) Representative FACS data of the frequency of TNF-α + cells within gated CD19 + CD24 hi CD27 + Bregs from PBMCs of BP patients and healthy controls. APC/Cy7 Mouse IgG1 were used as a negative control for immunofluorescence staining and flow cytometry assay. ( C ) Statistical analysis of the frequency of TNF-α + cells within gated CD19 + CD24 hi CD27 + Bregs in the indicated groups (n = 8 per groups). ( D ) Anti-BP180 antibody production in patient-derived PBMCs treated with etanercept. *p
    Pbmcs, supplied by Pfizer Inc, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbmcs/product/Pfizer Inc
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    pbmcs - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    85
    Pfizer Inc sm pbmc pha stimulated sm pbmc
    Effect of CCR5 blocking on SIVsmm use of CCR5 and entry into primary SM <t>PBMC.</t> (A) 293T cells were transfected with CD4 in combination with wild-type smCCR5 or GPR15. Two days post-transfection, target cells were pretreated for one hour with or without the CCR5 antagonist, maraviroc (15uM), and then infected with pseudotype virions carrying SIVsmm Envs from a CCR5 wild-type animal (FFv) and a CCR5-null animal (FNp). Three days later, infection was measured based on RLU (mean ± SD) in cell lysates. (B) Growth curves from infection of primary SM PBMC (FAk: W/W SM; FAz: Δ2/Δ2 SM). Cells were stimulated for 3 days with <t>PHA,</t> then pretreated for one hour with or without maraviroc (15 uM), followed by infection with two different SIVsmm primary isolates (M923 and M951) in the continued presence or absence of maraviroc. SIV Gag p27 antigen levels in viral supernatants were measured by ELISA.
    Sm Pbmc Pha Stimulated Sm Pbmc, supplied by Pfizer Inc, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sm pbmc pha stimulated sm pbmc/product/Pfizer Inc
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sm pbmc pha stimulated sm pbmc - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

    Image Search Results


    Modified function of Bregs were due to the expression of TNF-α. ( A ) Levels of TNF-α, IFN-γ, IL-10, IL-4, IL-22 and IL-23 mRNA in CD19 + CD24 hi CD27 + Bregs after sorting from PBMCs of 5 patients and 4 healthy controls. ( B) Representative FACS data of the frequency of TNF-α + cells within gated CD19 + CD24 hi CD27 + Bregs from PBMCs of BP patients and healthy controls. APC/Cy7 Mouse IgG1 were used as a negative control for immunofluorescence staining and flow cytometry assay. ( C ) Statistical analysis of the frequency of TNF-α + cells within gated CD19 + CD24 hi CD27 + Bregs in the indicated groups (n = 8 per groups). ( D ) Anti-BP180 antibody production in patient-derived PBMCs treated with etanercept. *p

    Journal: Scientific Reports

    Article Title: Dysfunction of CD19+CD24hiCD27+ B regulatory cells in patients with bullous pemphigoid

    doi: 10.1038/s41598-018-19226-z

    Figure Lengend Snippet: Modified function of Bregs were due to the expression of TNF-α. ( A ) Levels of TNF-α, IFN-γ, IL-10, IL-4, IL-22 and IL-23 mRNA in CD19 + CD24 hi CD27 + Bregs after sorting from PBMCs of 5 patients and 4 healthy controls. ( B) Representative FACS data of the frequency of TNF-α + cells within gated CD19 + CD24 hi CD27 + Bregs from PBMCs of BP patients and healthy controls. APC/Cy7 Mouse IgG1 were used as a negative control for immunofluorescence staining and flow cytometry assay. ( C ) Statistical analysis of the frequency of TNF-α + cells within gated CD19 + CD24 hi CD27 + Bregs in the indicated groups (n = 8 per groups). ( D ) Anti-BP180 antibody production in patient-derived PBMCs treated with etanercept. *p

    Article Snippet: To investigate the role of TNF-α in autoantibody production, patient-derived PBMCs were treated with etanercept (Pfizer, USA) to block the effect of TNF-α.

    Techniques: Modification, Expressing, FACS, Negative Control, Immunofluorescence, Staining, Flow Cytometry, Cytometry, Derivative Assay

    Modified function of Bregs were due to the expression of TNF-α. ( A ) Levels of TNF-α, IFN-γ, IL-10, IL-4, IL-22 and IL-23 mRNA in CD19 + CD24 hi CD27 + Bregs after sorting from PBMCs of 5 patients and 4 healthy controls. ( B) Representative FACS data of the frequency of TNF-α + cells within gated CD19 + CD24 hi CD27 + Bregs from PBMCs of BP patients and healthy controls. APC/Cy7 Mouse IgG1 were used as a negative control for immunofluorescence staining and flow cytometry assay. ( C ) Statistical analysis of the frequency of TNF-α + cells within gated CD19 + CD24 hi CD27 + Bregs in the indicated groups (n = 8 per groups). ( D ) Anti-BP180 antibody production in patient-derived PBMCs treated with etanercept. *p

    Journal: Scientific Reports

    Article Title: Dysfunction of CD19+CD24hiCD27+ B regulatory cells in patients with bullous pemphigoid

    doi: 10.1038/s41598-018-19226-z

    Figure Lengend Snippet: Modified function of Bregs were due to the expression of TNF-α. ( A ) Levels of TNF-α, IFN-γ, IL-10, IL-4, IL-22 and IL-23 mRNA in CD19 + CD24 hi CD27 + Bregs after sorting from PBMCs of 5 patients and 4 healthy controls. ( B) Representative FACS data of the frequency of TNF-α + cells within gated CD19 + CD24 hi CD27 + Bregs from PBMCs of BP patients and healthy controls. APC/Cy7 Mouse IgG1 were used as a negative control for immunofluorescence staining and flow cytometry assay. ( C ) Statistical analysis of the frequency of TNF-α + cells within gated CD19 + CD24 hi CD27 + Bregs in the indicated groups (n = 8 per groups). ( D ) Anti-BP180 antibody production in patient-derived PBMCs treated with etanercept. *p

    Article Snippet: To investigate the role of TNF-α in autoantibody production, patient-derived PBMCs were treated with etanercept (Pfizer, USA) to block the effect of TNF-α.

    Techniques: Modification, Expressing, FACS, Negative Control, Immunofluorescence, Staining, Flow Cytometry, Cytometry, Derivative Assay

    Comparison between wild-type strains and lpxL1 mutants in pro-inflammatory cytokine induction in PBMCs. PBMCs from three different donors were stimulated with titrations of the indicated strains and IL-6, TNF-α, and IL-1β were quantified in the supernatant 18 h after stimulation. H44/76 is a wild-type strain, L8 lpxL1 is a constructed lpxL1 mutant, pLAK33 is an LPS-deficient mutant, and all other strains are spontaneous lpxL1 mutants. Results of one representative experiment of two independent experiments are shown. Error bars indicate S.E.M. of triplicates.

    Journal: PLoS Pathogens

    Article Title: Naturally Occurring Lipid A Mutants in Neisseria meningitidis from Patients with Invasive Meningococcal Disease Are Associated with Reduced Coagulopathy

    doi: 10.1371/journal.ppat.1000396

    Figure Lengend Snippet: Comparison between wild-type strains and lpxL1 mutants in pro-inflammatory cytokine induction in PBMCs. PBMCs from three different donors were stimulated with titrations of the indicated strains and IL-6, TNF-α, and IL-1β were quantified in the supernatant 18 h after stimulation. H44/76 is a wild-type strain, L8 lpxL1 is a constructed lpxL1 mutant, pLAK33 is an LPS-deficient mutant, and all other strains are spontaneous lpxL1 mutants. Results of one representative experiment of two independent experiments are shown. Error bars indicate S.E.M. of triplicates.

    Article Snippet: Cell lines and PBMCs PBMC from HLA-oligotyped donors after leukapheresis were isolated by centrifugation of buffy coat cells on Ficoll-Hypaque (Pfizer) and were used after cryopreservation.

    Techniques: Construct, Mutagenesis

    Effect of CCR5 blocking on SIVsmm use of CCR5 and entry into primary SM PBMC. (A) 293T cells were transfected with CD4 in combination with wild-type smCCR5 or GPR15. Two days post-transfection, target cells were pretreated for one hour with or without the CCR5 antagonist, maraviroc (15uM), and then infected with pseudotype virions carrying SIVsmm Envs from a CCR5 wild-type animal (FFv) and a CCR5-null animal (FNp). Three days later, infection was measured based on RLU (mean ± SD) in cell lysates. (B) Growth curves from infection of primary SM PBMC (FAk: W/W SM; FAz: Δ2/Δ2 SM). Cells were stimulated for 3 days with PHA, then pretreated for one hour with or without maraviroc (15 uM), followed by infection with two different SIVsmm primary isolates (M923 and M951) in the continued presence or absence of maraviroc. SIV Gag p27 antigen levels in viral supernatants were measured by ELISA.

    Journal: PLoS Pathogens

    Article Title: A Novel CCR5 Mutation Common in Sooty Mangabeys Reveals SIVsmm Infection of CCR5-Null Natural Hosts and Efficient Alternative Coreceptor Use In Vivo

    doi: 10.1371/journal.ppat.1001064

    Figure Lengend Snippet: Effect of CCR5 blocking on SIVsmm use of CCR5 and entry into primary SM PBMC. (A) 293T cells were transfected with CD4 in combination with wild-type smCCR5 or GPR15. Two days post-transfection, target cells were pretreated for one hour with or without the CCR5 antagonist, maraviroc (15uM), and then infected with pseudotype virions carrying SIVsmm Envs from a CCR5 wild-type animal (FFv) and a CCR5-null animal (FNp). Three days later, infection was measured based on RLU (mean ± SD) in cell lysates. (B) Growth curves from infection of primary SM PBMC (FAk: W/W SM; FAz: Δ2/Δ2 SM). Cells were stimulated for 3 days with PHA, then pretreated for one hour with or without maraviroc (15 uM), followed by infection with two different SIVsmm primary isolates (M923 and M951) in the continued presence or absence of maraviroc. SIV Gag p27 antigen levels in viral supernatants were measured by ELISA.

    Article Snippet: SIV infections of primary SM PBMC PHA-stimulated SM PBMC were plated in 96-well plates at 2.5×105 cells/well, incubated for 1 hour in the presence or absence of the CCR5 antagonist maraviroc (15 uM; Pfizer), and then infected with SIVsmm strains (M923 and M951) by spin inoculation (1200×g for 2 hours) followed by overnight incubation.

    Techniques: Blocking Assay, Transfection, Infection, Enzyme-linked Immunosorbent Assay