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GraphPad Prism Inc pbmc
Pbmc, supplied by GraphPad Prism Inc, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pbmc/product/GraphPad Prism Inc
Average 92 stars, based on 2 article reviews
Price from $9.99 to $1999.99
pbmc - by Bioz Stars, 2020-09
92/100 stars

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Co-Culture Assay:

Article Title: Low Immunogenicity of Neural Progenitor Cells Differentiated from Induced Pluripotent Stem Cells Derived from Less Immunogenic Somatic Cells
Article Snippet: .. Proliferation of PBMCs at the different ratio of responding lymphocytes and stimulator cells. (A) Proliferation of PBMCs in target cells/PBMCs co-culture system at the ratio of 1∶100 and 1∶1000. (B) Degree value of PBMCs proliferation stimulated by target cells at different ratios. (The raw data used to create Figure S2A with the software Graphpad Prism 5.0.) (PDF) Click here for additional data file. .. Comparative analysis of HLA-A/B/C and HLA-DPB/DQB/DRA expression in SF-NPC and UMC-NPC after IFN-γ treatment.

Purification:

Article Title: Evidence of dysregulation of dendritic cells in primary HIV infection
Article Snippet: .. The nonparametric Mann-Whitney U test (2-tailed) was used for the cross-sectional comparison of DC subset numbers within PBMC and data on stimulation of allogeneic T-cell proliferation and cytokine/chemokine production and up-regulation of maturation markers after stimulation of purified DC subsets with TLR agonists in groups of PHIV and control subjects (HIV-infected and uninfected) using GraphPad Prism 4 (GraphPad Software). ..

Two Tailed Test:

Article Title: Enhancement of Human Immunodeficiency Virus (HIV)-Specific CD8+ T Cells in Cerebrospinal Fluid Compared to Those in Blood among Antiretroviral Therapy-Na?ve HIV-Positive Subjects ▿
Article Snippet: .. All comparisons between CSFMCs and PBMCs, including numbers of epitopes recognized, were performed using two-tailed Wilcoxon matched-pairs test with GraphPad Prism 5. ..

Transmigration Assay:

Article Title: Ethanol suppression of peripheral blood mononuclear cell trafficking across brain endothelial cells in immunodeficiency virus infection
Article Snippet: .. This was followed by Mann–Whitney analysis of data pairs (P < 0.05) to further evaluate changes in adherence of PBMCs and transmigration within each PBMC subset (eg, CD4 t cell transmigration associated with untreated BECs vs ethanol-treated BECs or CD8 T cell transmigration associated with ethanol-treated BECs+A and ethanol-treated BECS+MG) (GraphPad Prism® software; GraphPad, La Jolla, CA, USA). ..

Expressing:

Article Title: CD4 CD8 Double Positive T cell responses during Mycobacterium tuberculosis infection in cynomolgus macaques, et al. CD4CD8 Double Positive T cell responses during Mycobacterium tuberculosis infection in cynomolgus macaques
Article Snippet: .. Kruskal‐Wallis test with Dunn's multiple comparison test was used to compare changes in cell type, cytokine production, and cytolytic marker expression over time within BAL and PBMC (GraphPad Prism 7.0d statistical software). .. Wilcoxon method (with unadjusted P ‐values) was used to compare frequencies of CD4, CD8, and CD4CD8 T cells in PBMC and BAL and granulomas within the same time points (JMP Pro 12.1.0 statistical software).

Marker:

Article Title: CD4 CD8 Double Positive T cell responses during Mycobacterium tuberculosis infection in cynomolgus macaques, et al. CD4CD8 Double Positive T cell responses during Mycobacterium tuberculosis infection in cynomolgus macaques
Article Snippet: .. Kruskal‐Wallis test with Dunn's multiple comparison test was used to compare changes in cell type, cytokine production, and cytolytic marker expression over time within BAL and PBMC (GraphPad Prism 7.0d statistical software). .. Wilcoxon method (with unadjusted P ‐values) was used to compare frequencies of CD4, CD8, and CD4CD8 T cells in PBMC and BAL and granulomas within the same time points (JMP Pro 12.1.0 statistical software).

MANN-WHITNEY:

Article Title: Evidence of dysregulation of dendritic cells in primary HIV infection
Article Snippet: .. The nonparametric Mann-Whitney U test (2-tailed) was used for the cross-sectional comparison of DC subset numbers within PBMC and data on stimulation of allogeneic T-cell proliferation and cytokine/chemokine production and up-regulation of maturation markers after stimulation of purified DC subsets with TLR agonists in groups of PHIV and control subjects (HIV-infected and uninfected) using GraphPad Prism 4 (GraphPad Software). ..

Article Title: Impact of S. aureus USA300 Colonization and Skin Infections on Systemic Immune Responses in Humans
Article Snippet: .. Statistical analyses on cytokines from PBMCs were performed using the non-parametric Mann-Whitney test and GraphPadPrism 6.0 software. .. Analyses of serum cytokines were performed using Wilcoxon rank-sum test, or Wilcoxon rank-sum test for matched pairs when appropriate.

Article Title: Ethanol suppression of peripheral blood mononuclear cell trafficking across brain endothelial cells in immunodeficiency virus infection
Article Snippet: .. This was followed by Mann–Whitney analysis of data pairs (P < 0.05) to further evaluate changes in adherence of PBMCs and transmigration within each PBMC subset (eg, CD4 t cell transmigration associated with untreated BECs vs ethanol-treated BECs or CD8 T cell transmigration associated with ethanol-treated BECs+A and ethanol-treated BECS+MG) (GraphPad Prism® software; GraphPad, La Jolla, CA, USA). ..

other:

Article Title: Sequence-specific activation of the DNA sensor cGAS by Y-form DNA structures as found in primary HIV-1 cDNA
Article Snippet: If not stated otherwise, statistical analysis was performed by repeated-measures two-sided, one-way ANOVA with data matched according to donor (PBMCs) or the same experiment (cell lines) with GraphPad Prism 6.

Software:

Article Title: CD4 CD8 Double Positive T cell responses during Mycobacterium tuberculosis infection in cynomolgus macaques, et al. CD4CD8 Double Positive T cell responses during Mycobacterium tuberculosis infection in cynomolgus macaques
Article Snippet: .. Kruskal‐Wallis test with Dunn's multiple comparison test was used to compare changes in cell type, cytokine production, and cytolytic marker expression over time within BAL and PBMC (GraphPad Prism 7.0d statistical software). .. Wilcoxon method (with unadjusted P ‐values) was used to compare frequencies of CD4, CD8, and CD4CD8 T cells in PBMC and BAL and granulomas within the same time points (JMP Pro 12.1.0 statistical software).

Article Title: Impact of S. aureus USA300 Colonization and Skin Infections on Systemic Immune Responses in Humans
Article Snippet: .. Statistical analyses on cytokines from PBMCs were performed using the non-parametric Mann-Whitney test and GraphPadPrism 6.0 software. .. Analyses of serum cytokines were performed using Wilcoxon rank-sum test, or Wilcoxon rank-sum test for matched pairs when appropriate.

Article Title: Low Immunogenicity of Neural Progenitor Cells Differentiated from Induced Pluripotent Stem Cells Derived from Less Immunogenic Somatic Cells
Article Snippet: .. Proliferation of PBMCs at the different ratio of responding lymphocytes and stimulator cells. (A) Proliferation of PBMCs in target cells/PBMCs co-culture system at the ratio of 1∶100 and 1∶1000. (B) Degree value of PBMCs proliferation stimulated by target cells at different ratios. (The raw data used to create Figure S2A with the software Graphpad Prism 5.0.) (PDF) Click here for additional data file. .. Comparative analysis of HLA-A/B/C and HLA-DPB/DQB/DRA expression in SF-NPC and UMC-NPC after IFN-γ treatment.

Article Title: Ethanol suppression of peripheral blood mononuclear cell trafficking across brain endothelial cells in immunodeficiency virus infection
Article Snippet: .. This was followed by Mann–Whitney analysis of data pairs (P < 0.05) to further evaluate changes in adherence of PBMCs and transmigration within each PBMC subset (eg, CD4 t cell transmigration associated with untreated BECs vs ethanol-treated BECs or CD8 T cell transmigration associated with ethanol-treated BECs+A and ethanol-treated BECS+MG) (GraphPad Prism® software; GraphPad, La Jolla, CA, USA). ..

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  • 85
    GraphPad Prism Inc pbmcs co culture system
    <t>Perforin</t> and granzyme B expressions in <t>PBMCs</t> co-culture system with NPC stimulation. (A) Analysis of surface phenotypic markers (CD3, CD8, CD56) of T cells and NK cells. PBMCs (n = 20) co-cultured with SF-NPC or UMC-NPC were labeled with different fluorescence-conjugated antibodies, dividing into three obvious cell groups (CD3+CD8− T cells, CD3+CD8+ T cells and CD3−CD56+ NK cells) monitored by flow cytometry analysis. (B and C) Analysis of perforin and granzyme B expression in T cells and NK cells. Differences between groups were analyzed by paired-samples t -tests ( P
    Pbmcs Co Culture System, supplied by GraphPad Prism Inc, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbmcs co culture system/product/GraphPad Prism Inc
    Average 85 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    pbmcs co culture system - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

    85
    GraphPad Prism Inc pbmc expressing cell surface tlr9
    Cell surface <t>TLR9</t> expression on human <t>PBMC</t> is up-regulated following LPS stimulation. Human PBMC were cultured for 24 h in 30, 10, or 1 μg of LPS per ml and then stained with the murine anti-human TLR9-APC MAb or the isotype control. The histogram
    Pbmc Expressing Cell Surface Tlr9, supplied by GraphPad Prism Inc, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbmc expressing cell surface tlr9/product/GraphPad Prism Inc
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pbmc expressing cell surface tlr9 - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

    85
    GraphPad Prism Inc in vitro pbmc stimulation
    Functional analysis on the stimulation of the peripheral blood mononuclear cells <t>(PBMCs)</t> with heat-killed Candida albicans blastoconidia. <t>Cytokine</t> production capacity of tumor necrosis factor alpha (TNFα), interleukin (IL)–1β,
    In Vitro Pbmc Stimulation, supplied by GraphPad Prism Inc, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/in vitro pbmc stimulation/product/GraphPad Prism Inc
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    in vitro pbmc stimulation - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

    Image Search Results


    Perforin and granzyme B expressions in PBMCs co-culture system with NPC stimulation. (A) Analysis of surface phenotypic markers (CD3, CD8, CD56) of T cells and NK cells. PBMCs (n = 20) co-cultured with SF-NPC or UMC-NPC were labeled with different fluorescence-conjugated antibodies, dividing into three obvious cell groups (CD3+CD8− T cells, CD3+CD8+ T cells and CD3−CD56+ NK cells) monitored by flow cytometry analysis. (B and C) Analysis of perforin and granzyme B expression in T cells and NK cells. Differences between groups were analyzed by paired-samples t -tests ( P

    Journal: PLoS ONE

    Article Title: Low Immunogenicity of Neural Progenitor Cells Differentiated from Induced Pluripotent Stem Cells Derived from Less Immunogenic Somatic Cells

    doi: 10.1371/journal.pone.0069617

    Figure Lengend Snippet: Perforin and granzyme B expressions in PBMCs co-culture system with NPC stimulation. (A) Analysis of surface phenotypic markers (CD3, CD8, CD56) of T cells and NK cells. PBMCs (n = 20) co-cultured with SF-NPC or UMC-NPC were labeled with different fluorescence-conjugated antibodies, dividing into three obvious cell groups (CD3+CD8− T cells, CD3+CD8+ T cells and CD3−CD56+ NK cells) monitored by flow cytometry analysis. (B and C) Analysis of perforin and granzyme B expression in T cells and NK cells. Differences between groups were analyzed by paired-samples t -tests ( P

    Article Snippet: Percentage of perforin expression in various immune effector cells in PBMCs co-culture system. (The raw data used to create with the software Graphpad Prism 5.0.) (PDF) Click here for additional data file.

    Techniques: Co-Culture Assay, Cell Culture, Labeling, Fluorescence, Flow Cytometry, Cytometry, Expressing

    Frequencies of HIV-specific tetramer-positive cells among ex vivo expanded cells. CSFMCs and PBMCs were expanded ex vivo for 2 weeks with PHA and then assessed for frequencies of HIV-specific tetramer-positive CD8 + cells. (A) Representative flow

    Journal:

    Article Title: Enhancement of Human Immunodeficiency Virus (HIV)-Specific CD8+ T Cells in Cerebrospinal Fluid Compared to Those in Blood among Antiretroviral Therapy-Na?ve HIV-Positive Subjects ▿

    doi: 10.1128/JVI.01190-08

    Figure Lengend Snippet: Frequencies of HIV-specific tetramer-positive cells among ex vivo expanded cells. CSFMCs and PBMCs were expanded ex vivo for 2 weeks with PHA and then assessed for frequencies of HIV-specific tetramer-positive CD8 + cells. (A) Representative flow

    Article Snippet: All comparisons between CSFMCs and PBMCs, including numbers of epitopes recognized, were performed using two-tailed Wilcoxon matched-pairs test with GraphPad Prism 5.

    Techniques: Ex Vivo, Flow Cytometry

    Frequencies of IFN-γ-secreting cells, as assessed by ELISPOT assay. CSFMCs and PBMCs were expanded for 2 weeks with PHA and then assessed for frequency of IFN-γ SFC to HIV-specific optimal class I peptides by ELISPOT assay. Numbers of

    Journal:

    Article Title: Enhancement of Human Immunodeficiency Virus (HIV)-Specific CD8+ T Cells in Cerebrospinal Fluid Compared to Those in Blood among Antiretroviral Therapy-Na?ve HIV-Positive Subjects ▿

    doi: 10.1128/JVI.01190-08

    Figure Lengend Snippet: Frequencies of IFN-γ-secreting cells, as assessed by ELISPOT assay. CSFMCs and PBMCs were expanded for 2 weeks with PHA and then assessed for frequency of IFN-γ SFC to HIV-specific optimal class I peptides by ELISPOT assay. Numbers of

    Article Snippet: All comparisons between CSFMCs and PBMCs, including numbers of epitopes recognized, were performed using two-tailed Wilcoxon matched-pairs test with GraphPad Prism 5.

    Techniques: Enzyme-linked Immunospot

    Cell surface TLR9 expression on human PBMC is up-regulated following LPS stimulation. Human PBMC were cultured for 24 h in 30, 10, or 1 μg of LPS per ml and then stained with the murine anti-human TLR9-APC MAb or the isotype control. The histogram

    Journal:

    Article Title: Toll-Like Receptor 9 Can Be Expressed at the Cell Surface of Distinct Populations of Tonsils and Human Peripheral Blood Mononuclear Cells

    doi: 10.1128/IAI.72.12.7202-7211.2004

    Figure Lengend Snippet: Cell surface TLR9 expression on human PBMC is up-regulated following LPS stimulation. Human PBMC were cultured for 24 h in 30, 10, or 1 μg of LPS per ml and then stained with the murine anti-human TLR9-APC MAb or the isotype control. The histogram

    Article Snippet: Populations of PBMC expressing cell surface TLR9 before and after culture were compared by using paired Student t tests with the software GraphPad Prism version 4.0.

    Techniques: Expressing, Cell Culture, Staining

    Up-regulation of cell surface TLR9 expression on human PBMC following LPS stimulation.

    Journal:

    Article Title: Toll-Like Receptor 9 Can Be Expressed at the Cell Surface of Distinct Populations of Tonsils and Human Peripheral Blood Mononuclear Cells

    doi: 10.1128/IAI.72.12.7202-7211.2004

    Figure Lengend Snippet: Up-regulation of cell surface TLR9 expression on human PBMC following LPS stimulation.

    Article Snippet: Populations of PBMC expressing cell surface TLR9 before and after culture were compared by using paired Student t tests with the software GraphPad Prism version 4.0.

    Techniques: Expressing

    Cell surface TLR9 is expressed on human PBMC. (A) A representative dot plot displays forward and side scatters of the PBMC used for live-cell gating (R1). (B) Staining with the TLR9 MAb (dark open line) is shown overlaying staining observed with the isotype

    Journal:

    Article Title: Toll-Like Receptor 9 Can Be Expressed at the Cell Surface of Distinct Populations of Tonsils and Human Peripheral Blood Mononuclear Cells

    doi: 10.1128/IAI.72.12.7202-7211.2004

    Figure Lengend Snippet: Cell surface TLR9 is expressed on human PBMC. (A) A representative dot plot displays forward and side scatters of the PBMC used for live-cell gating (R1). (B) Staining with the TLR9 MAb (dark open line) is shown overlaying staining observed with the isotype

    Article Snippet: Populations of PBMC expressing cell surface TLR9 before and after culture were compared by using paired Student t tests with the software GraphPad Prism version 4.0.

    Techniques: Staining

    TLR9 is expressed at the cell surface of distinct populations of tonsil cells and PBMC.

    Journal:

    Article Title: Toll-Like Receptor 9 Can Be Expressed at the Cell Surface of Distinct Populations of Tonsils and Human Peripheral Blood Mononuclear Cells

    doi: 10.1128/IAI.72.12.7202-7211.2004

    Figure Lengend Snippet: TLR9 is expressed at the cell surface of distinct populations of tonsil cells and PBMC.

    Article Snippet: Populations of PBMC expressing cell surface TLR9 before and after culture were compared by using paired Student t tests with the software GraphPad Prism version 4.0.

    Techniques:

    Forward and side scatter profiles of TLR9 positive cells in (A) tonsils and (B) human PBMC.

    Journal:

    Article Title: Toll-Like Receptor 9 Can Be Expressed at the Cell Surface of Distinct Populations of Tonsils and Human Peripheral Blood Mononuclear Cells

    doi: 10.1128/IAI.72.12.7202-7211.2004

    Figure Lengend Snippet: Forward and side scatter profiles of TLR9 positive cells in (A) tonsils and (B) human PBMC.

    Article Snippet: Populations of PBMC expressing cell surface TLR9 before and after culture were compared by using paired Student t tests with the software GraphPad Prism version 4.0.

    Techniques:

    TLR9 is expressed at the cell surface of distinct populations of tonsil cells and PBMC.

    Journal:

    Article Title: Toll-Like Receptor 9 Can Be Expressed at the Cell Surface of Distinct Populations of Tonsils and Human Peripheral Blood Mononuclear Cells

    doi: 10.1128/IAI.72.12.7202-7211.2004

    Figure Lengend Snippet: TLR9 is expressed at the cell surface of distinct populations of tonsil cells and PBMC.

    Article Snippet: Populations of PBMC expressing cell surface TLR9 before and after culture were compared by using paired Student t tests with the software GraphPad Prism version 4.0.

    Techniques:

    Cell surface TLR9 expression visualized by immunofluorescence staining. Double staining of LPS-stimulated human PBMC with (A) the APC-labeled isotype-matched MAbs and (B) FITC-labeled anti-CD19 MAbs, using the same microscopic field, and double staining

    Journal:

    Article Title: Toll-Like Receptor 9 Can Be Expressed at the Cell Surface of Distinct Populations of Tonsils and Human Peripheral Blood Mononuclear Cells

    doi: 10.1128/IAI.72.12.7202-7211.2004

    Figure Lengend Snippet: Cell surface TLR9 expression visualized by immunofluorescence staining. Double staining of LPS-stimulated human PBMC with (A) the APC-labeled isotype-matched MAbs and (B) FITC-labeled anti-CD19 MAbs, using the same microscopic field, and double staining

    Article Snippet: Populations of PBMC expressing cell surface TLR9 before and after culture were compared by using paired Student t tests with the software GraphPad Prism version 4.0.

    Techniques: Expressing, Immunofluorescence, Staining, Double Staining, Labeling

    Functional analysis on the stimulation of the peripheral blood mononuclear cells (PBMCs) with heat-killed Candida albicans blastoconidia. Cytokine production capacity of tumor necrosis factor alpha (TNFα), interleukin (IL)–1β,

    Journal: The Journal of Infectious Diseases

    Article Title: Genetic Variation in the Dectin-1/CARD9 Recognition Pathway and Susceptibility to Candidemia

    doi: 10.1093/infdis/jir458

    Figure Lengend Snippet: Functional analysis on the stimulation of the peripheral blood mononuclear cells (PBMCs) with heat-killed Candida albicans blastoconidia. Cytokine production capacity of tumor necrosis factor alpha (TNFα), interleukin (IL)–1β,

    Article Snippet: For the analysis of the in vitro PBMC stimulation and in vivo serum cytokine measurements, a Mann–Whitney U test was performed on the DECTIN-1 genotype experiment and a Kruskal–Wallis 1-way analysis of variance was performed on the CARD9 genotype experiment with the use of GraphPad Prism software version 4.00.

    Techniques: Functional Assay