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3H Biomedical pbmc
Expression of different uPAR splice variant mRNAs in different tissues/cell types normalised to total uPAR . A series of real-time PCR assays was used to measure the expression of different splice variants of uPAR in an extended panel of tissues/cell types (lung, brain, HASM, undifferentiated HBEC, BEAS2B, <t>PMN,</t> <t>PBMC,</t> THP1). Expression of each variant is shown as mean + SEM of three PCR replicates, for two donors or biological replicates as appropriate. Data are shown as 2 -ΔCt normalised to total uPAR and relative to a suitable plasmid control (designated 100%). (A) total classical uPAR (exon 7), (B) classical uPAR exon 6 deletion, (C) classical uPAR exons 5+6 deletion, (D) classical uPAR exon 3 deletion, (E) total alternative uPAR (exon7b), (F) alternative uPAR exon 4+5 deletion. Classical uPAR exon 5 and 4+5 deletions and alternative uPAR exon 5 deletion were not detected.
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Article Title: Characterisation of urokinase plasminogen activator receptor variants in human airway and peripheral cells

Journal: BMC Molecular Biology

doi: 10.1186/1471-2199-10-75

Expression of different uPAR splice variant mRNAs in different tissues/cell types normalised to total uPAR . A series of real-time PCR assays was used to measure the expression of different splice variants of uPAR in an extended panel of tissues/cell types (lung, brain, HASM, undifferentiated HBEC, BEAS2B, PMN, PBMC, THP1). Expression of each variant is shown as mean + SEM of three PCR replicates, for two donors or biological replicates as appropriate. Data are shown as 2 -ΔCt normalised to total uPAR and relative to a suitable plasmid control (designated 100%). (A) total classical uPAR (exon 7), (B) classical uPAR exon 6 deletion, (C) classical uPAR exons 5+6 deletion, (D) classical uPAR exon 3 deletion, (E) total alternative uPAR (exon7b), (F) alternative uPAR exon 4+5 deletion. Classical uPAR exon 5 and 4+5 deletions and alternative uPAR exon 5 deletion were not detected.
Figure Legend Snippet: Expression of different uPAR splice variant mRNAs in different tissues/cell types normalised to total uPAR . A series of real-time PCR assays was used to measure the expression of different splice variants of uPAR in an extended panel of tissues/cell types (lung, brain, HASM, undifferentiated HBEC, BEAS2B, PMN, PBMC, THP1). Expression of each variant is shown as mean + SEM of three PCR replicates, for two donors or biological replicates as appropriate. Data are shown as 2 -ΔCt normalised to total uPAR and relative to a suitable plasmid control (designated 100%). (A) total classical uPAR (exon 7), (B) classical uPAR exon 6 deletion, (C) classical uPAR exons 5+6 deletion, (D) classical uPAR exon 3 deletion, (E) total alternative uPAR (exon7b), (F) alternative uPAR exon 4+5 deletion. Classical uPAR exon 5 and 4+5 deletions and alternative uPAR exon 5 deletion were not detected.

Techniques Used: Expressing, Variant Assay, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction, Plasmid Preparation

Expression of uPAR splice variant mRNA in different tissues/cell types . A series of real-time PCR (TaqMan) assays was used to measure the expression of different splice variants of uPAR in an extended panel of tissues/cell types (lung, brain, HASM, undifferentiated HBEC, BEAS2B, PMN, PBMC, THP1). Expression of each variant is shown as mean + SEM of three PCR replicates, for two donors or biological replicates as appropriate. Data are shown as 2 -ΔCt normalised to HPRT1 and relative to a suitable plasmid positive control containing the specific splice variant cDNA (designated 100%). (A) total uPAR, (B) total classical uPAR (exon 7), (C) classical uPAR exon 6 deletion, (D) classical uPAR exons 5+6 deletion, (E) classical uPAR exon 3 deletion, (F) total alternative uPAR (exon7b), (G) alternative uPAR exon 4+5 deletion. Classical uPAR exon 5 and 4+5 deletions and alternative uPAR exon 5 deletion were not detected.
Figure Legend Snippet: Expression of uPAR splice variant mRNA in different tissues/cell types . A series of real-time PCR (TaqMan) assays was used to measure the expression of different splice variants of uPAR in an extended panel of tissues/cell types (lung, brain, HASM, undifferentiated HBEC, BEAS2B, PMN, PBMC, THP1). Expression of each variant is shown as mean + SEM of three PCR replicates, for two donors or biological replicates as appropriate. Data are shown as 2 -ΔCt normalised to HPRT1 and relative to a suitable plasmid positive control containing the specific splice variant cDNA (designated 100%). (A) total uPAR, (B) total classical uPAR (exon 7), (C) classical uPAR exon 6 deletion, (D) classical uPAR exons 5+6 deletion, (E) classical uPAR exon 3 deletion, (F) total alternative uPAR (exon7b), (G) alternative uPAR exon 4+5 deletion. Classical uPAR exon 5 and 4+5 deletions and alternative uPAR exon 5 deletion were not detected.

Techniques Used: Expressing, Variant Assay, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction, Plasmid Preparation, Positive Control

Expression of uPAR protein in different cell types . Western blotting of cell lysates was performed for an extended panel of cell types (HASM, undifferentiated HBEC, BEAS2B, THP1, PMN, PBMC) plus recombinant uPAR (ruPAR) using domain I specific (A) and domain II specific (B) antibodies to identify different variants. A β-actin antibody was used as a loading control (C). An ELISA assay, with a sensitivity of 30 pg/mL, was used to detect soluble uPAR in the culture supernatant where appropriate (D).
Figure Legend Snippet: Expression of uPAR protein in different cell types . Western blotting of cell lysates was performed for an extended panel of cell types (HASM, undifferentiated HBEC, BEAS2B, THP1, PMN, PBMC) plus recombinant uPAR (ruPAR) using domain I specific (A) and domain II specific (B) antibodies to identify different variants. A β-actin antibody was used as a loading control (C). An ELISA assay, with a sensitivity of 30 pg/mL, was used to detect soluble uPAR in the culture supernatant where appropriate (D).

Techniques Used: Expressing, Western Blot, Recombinant, Enzyme-linked Immunosorbent Assay

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    3H Biomedical pbmc
    Expression of different uPAR splice variant mRNAs in different tissues/cell types normalised to total uPAR . A series of real-time PCR assays was used to measure the expression of different splice variants of uPAR in an extended panel of tissues/cell types (lung, brain, HASM, undifferentiated HBEC, BEAS2B, <t>PMN,</t> <t>PBMC,</t> THP1). Expression of each variant is shown as mean + SEM of three PCR replicates, for two donors or biological replicates as appropriate. Data are shown as 2 -ΔCt normalised to total uPAR and relative to a suitable plasmid control (designated 100%). (A) total classical uPAR (exon 7), (B) classical uPAR exon 6 deletion, (C) classical uPAR exons 5+6 deletion, (D) classical uPAR exon 3 deletion, (E) total alternative uPAR (exon7b), (F) alternative uPAR exon 4+5 deletion. Classical uPAR exon 5 and 4+5 deletions and alternative uPAR exon 5 deletion were not detected.
    Pbmc, supplied by 3H Biomedical, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbmc/product/3H Biomedical
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pbmc - by Bioz Stars, 2020-09
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    3H Biomedical human pbmcs
    IgGH is transported to the cell surface by HLA-DR. ( A ) The secreted forms of <t>IgGHs</t> cloned from human <t>PBMCs</t> that have different V regions were cotransfected with HLA-DRα, DRB1*04:04 (HLA-DR4), and GFP. Cell surface IgG on GFP-expressing cells was
    Human Pbmcs, supplied by 3H Biomedical, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    3H Biomedical blood mononuclear cells pbmc
    IgGH is transported to the cell surface by HLA-DR. ( A ) The secreted forms of <t>IgGHs</t> cloned from human <t>PBMCs</t> that have different V regions were cotransfected with HLA-DRα, DRB1*04:04 (HLA-DR4), and GFP. Cell surface IgG on GFP-expressing cells was
    Blood Mononuclear Cells Pbmc, supplied by 3H Biomedical, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Expression of different uPAR splice variant mRNAs in different tissues/cell types normalised to total uPAR . A series of real-time PCR assays was used to measure the expression of different splice variants of uPAR in an extended panel of tissues/cell types (lung, brain, HASM, undifferentiated HBEC, BEAS2B, PMN, PBMC, THP1). Expression of each variant is shown as mean + SEM of three PCR replicates, for two donors or biological replicates as appropriate. Data are shown as 2 -ΔCt normalised to total uPAR and relative to a suitable plasmid control (designated 100%). (A) total classical uPAR (exon 7), (B) classical uPAR exon 6 deletion, (C) classical uPAR exons 5+6 deletion, (D) classical uPAR exon 3 deletion, (E) total alternative uPAR (exon7b), (F) alternative uPAR exon 4+5 deletion. Classical uPAR exon 5 and 4+5 deletions and alternative uPAR exon 5 deletion were not detected.

    Journal: BMC Molecular Biology

    Article Title: Characterisation of urokinase plasminogen activator receptor variants in human airway and peripheral cells

    doi: 10.1186/1471-2199-10-75

    Figure Lengend Snippet: Expression of different uPAR splice variant mRNAs in different tissues/cell types normalised to total uPAR . A series of real-time PCR assays was used to measure the expression of different splice variants of uPAR in an extended panel of tissues/cell types (lung, brain, HASM, undifferentiated HBEC, BEAS2B, PMN, PBMC, THP1). Expression of each variant is shown as mean + SEM of three PCR replicates, for two donors or biological replicates as appropriate. Data are shown as 2 -ΔCt normalised to total uPAR and relative to a suitable plasmid control (designated 100%). (A) total classical uPAR (exon 7), (B) classical uPAR exon 6 deletion, (C) classical uPAR exons 5+6 deletion, (D) classical uPAR exon 3 deletion, (E) total alternative uPAR (exon7b), (F) alternative uPAR exon 4+5 deletion. Classical uPAR exon 5 and 4+5 deletions and alternative uPAR exon 5 deletion were not detected.

    Article Snippet: RNA and lysates from PMN and PBMC were also obtained commercially (3 H Biomedical, Uppsala, Sweden).

    Techniques: Expressing, Variant Assay, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction, Plasmid Preparation

    Expression of uPAR splice variant mRNA in different tissues/cell types . A series of real-time PCR (TaqMan) assays was used to measure the expression of different splice variants of uPAR in an extended panel of tissues/cell types (lung, brain, HASM, undifferentiated HBEC, BEAS2B, PMN, PBMC, THP1). Expression of each variant is shown as mean + SEM of three PCR replicates, for two donors or biological replicates as appropriate. Data are shown as 2 -ΔCt normalised to HPRT1 and relative to a suitable plasmid positive control containing the specific splice variant cDNA (designated 100%). (A) total uPAR, (B) total classical uPAR (exon 7), (C) classical uPAR exon 6 deletion, (D) classical uPAR exons 5+6 deletion, (E) classical uPAR exon 3 deletion, (F) total alternative uPAR (exon7b), (G) alternative uPAR exon 4+5 deletion. Classical uPAR exon 5 and 4+5 deletions and alternative uPAR exon 5 deletion were not detected.

    Journal: BMC Molecular Biology

    Article Title: Characterisation of urokinase plasminogen activator receptor variants in human airway and peripheral cells

    doi: 10.1186/1471-2199-10-75

    Figure Lengend Snippet: Expression of uPAR splice variant mRNA in different tissues/cell types . A series of real-time PCR (TaqMan) assays was used to measure the expression of different splice variants of uPAR in an extended panel of tissues/cell types (lung, brain, HASM, undifferentiated HBEC, BEAS2B, PMN, PBMC, THP1). Expression of each variant is shown as mean + SEM of three PCR replicates, for two donors or biological replicates as appropriate. Data are shown as 2 -ΔCt normalised to HPRT1 and relative to a suitable plasmid positive control containing the specific splice variant cDNA (designated 100%). (A) total uPAR, (B) total classical uPAR (exon 7), (C) classical uPAR exon 6 deletion, (D) classical uPAR exons 5+6 deletion, (E) classical uPAR exon 3 deletion, (F) total alternative uPAR (exon7b), (G) alternative uPAR exon 4+5 deletion. Classical uPAR exon 5 and 4+5 deletions and alternative uPAR exon 5 deletion were not detected.

    Article Snippet: RNA and lysates from PMN and PBMC were also obtained commercially (3 H Biomedical, Uppsala, Sweden).

    Techniques: Expressing, Variant Assay, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction, Plasmid Preparation, Positive Control

    Expression of uPAR protein in different cell types . Western blotting of cell lysates was performed for an extended panel of cell types (HASM, undifferentiated HBEC, BEAS2B, THP1, PMN, PBMC) plus recombinant uPAR (ruPAR) using domain I specific (A) and domain II specific (B) antibodies to identify different variants. A β-actin antibody was used as a loading control (C). An ELISA assay, with a sensitivity of 30 pg/mL, was used to detect soluble uPAR in the culture supernatant where appropriate (D).

    Journal: BMC Molecular Biology

    Article Title: Characterisation of urokinase plasminogen activator receptor variants in human airway and peripheral cells

    doi: 10.1186/1471-2199-10-75

    Figure Lengend Snippet: Expression of uPAR protein in different cell types . Western blotting of cell lysates was performed for an extended panel of cell types (HASM, undifferentiated HBEC, BEAS2B, THP1, PMN, PBMC) plus recombinant uPAR (ruPAR) using domain I specific (A) and domain II specific (B) antibodies to identify different variants. A β-actin antibody was used as a loading control (C). An ELISA assay, with a sensitivity of 30 pg/mL, was used to detect soluble uPAR in the culture supernatant where appropriate (D).

    Article Snippet: RNA and lysates from PMN and PBMC were also obtained commercially (3 H Biomedical, Uppsala, Sweden).

    Techniques: Expressing, Western Blot, Recombinant, Enzyme-linked Immunosorbent Assay

    IgGH is transported to the cell surface by HLA-DR. ( A ) The secreted forms of IgGHs cloned from human PBMCs that have different V regions were cotransfected with HLA-DRα, DRB1*04:04 (HLA-DR4), and GFP. Cell surface IgG on GFP-expressing cells was

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Autoantibodies to IgG/HLA class II complexes are associated with rheumatoid arthritis susceptibility

    doi: 10.1073/pnas.1401105111

    Figure Lengend Snippet: IgGH is transported to the cell surface by HLA-DR. ( A ) The secreted forms of IgGHs cloned from human PBMCs that have different V regions were cotransfected with HLA-DRα, DRB1*04:04 (HLA-DR4), and GFP. Cell surface IgG on GFP-expressing cells was

    Article Snippet: IgGHs were amplified from human PBMCs (3H Biomedical) using sense primers for V regions (5′-GTCTTGTCCCAGGTCACCTTGAAGGAG-3′ for clone 1 and 3, 5′-GCCCACTCCCAGGTGCAGCTGGTGCAG-3′ for clone 2, 5′-GTGCAGCTGGTGCAGTCTGGAGCAGAG-3′ for clone 4, 5′-GTCCAGTGTGAAGTGCAGCTGGTGGAG-3′ for clone 5), and antisense primer for constant region of secreted IgG (5′-TCATTTACCCGGAGACAGGGAG-3′) and cloned into pME18S expression vector containing a CD150 signal sequence.

    Techniques: Clone Assay, Expressing