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Addgene inc pbgsa fpr1 egfp
Bifunctional compounds enhance phagocytosis of A. fumigatus conidia by zebrafish neutrophils expressing human <t>FPR1.</t> (A) (i) Diagram of experimental approach: Calcofluor-stained A. fumigatus conidia (blue) are co-delivered with treatments into the circulation via the Duct of Cuvier at 3 days post-fertilization. Imaging is performed at 2 h post-infection at a distal site, the caudal venous plexus, which is rich in leukocytes. (ii) Example image of 3 dpf irf8 -MO treated Tg( mpx <t>:EGFP)</t> larva (GFP-labeled neutrophils) with mosaic expression of human FPR1 (traced by mCherry, red fluorescence), 2 h following delivery of calcofluor-labeled (blue fluorescent) A. fumigatus conidia. A GFP/mCherry co-labeled leukocyte containing phagocytosed conidia is indicated in magnified panel (open white arrowhead). Off-target expression of the transgene was also observed in tissues including the somites (full white arrowhead). (B) Treatment with C-001 and C-016 resulted in significantly increased phagocytosis of conidia by GFP/mCherry+ (human FPR1-expressing) neutrophils compared to GFP(only) wild-type cells. Each point represents an infected larva. N ≥ 40 larva scored per condition. Data collated from multiple experiments. Statistics: two-tailed T -test. * p ≤ 0.05, *** p ≤ 0.001.
Pbgsa Fpr1 Egfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pbgsa fpr1 egfp/product/Addgene inc
Average 90 stars, based on 1 article reviews
Price from $9.99 to $1999.99
pbgsa fpr1 egfp - by Bioz Stars, 2024-04
90/100 stars

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1) Product Images from "Bifunctional Small Molecules Enhance Neutrophil Activities Against Aspergillus fumigatus in vivo and in vitro"

Article Title: Bifunctional Small Molecules Enhance Neutrophil Activities Against Aspergillus fumigatus in vivo and in vitro

Journal: Frontiers in Immunology

doi: 10.3389/fimmu.2019.00644

Bifunctional compounds enhance phagocytosis of A. fumigatus conidia by zebrafish neutrophils expressing human FPR1. (A) (i) Diagram of experimental approach: Calcofluor-stained A. fumigatus conidia (blue) are co-delivered with treatments into the circulation via the Duct of Cuvier at 3 days post-fertilization. Imaging is performed at 2 h post-infection at a distal site, the caudal venous plexus, which is rich in leukocytes. (ii) Example image of 3 dpf irf8 -MO treated Tg( mpx :EGFP) larva (GFP-labeled neutrophils) with mosaic expression of human FPR1 (traced by mCherry, red fluorescence), 2 h following delivery of calcofluor-labeled (blue fluorescent) A. fumigatus conidia. A GFP/mCherry co-labeled leukocyte containing phagocytosed conidia is indicated in magnified panel (open white arrowhead). Off-target expression of the transgene was also observed in tissues including the somites (full white arrowhead). (B) Treatment with C-001 and C-016 resulted in significantly increased phagocytosis of conidia by GFP/mCherry+ (human FPR1-expressing) neutrophils compared to GFP(only) wild-type cells. Each point represents an infected larva. N ≥ 40 larva scored per condition. Data collated from multiple experiments. Statistics: two-tailed T -test. * p ≤ 0.05, *** p ≤ 0.001.
Figure Legend Snippet: Bifunctional compounds enhance phagocytosis of A. fumigatus conidia by zebrafish neutrophils expressing human FPR1. (A) (i) Diagram of experimental approach: Calcofluor-stained A. fumigatus conidia (blue) are co-delivered with treatments into the circulation via the Duct of Cuvier at 3 days post-fertilization. Imaging is performed at 2 h post-infection at a distal site, the caudal venous plexus, which is rich in leukocytes. (ii) Example image of 3 dpf irf8 -MO treated Tg( mpx :EGFP) larva (GFP-labeled neutrophils) with mosaic expression of human FPR1 (traced by mCherry, red fluorescence), 2 h following delivery of calcofluor-labeled (blue fluorescent) A. fumigatus conidia. A GFP/mCherry co-labeled leukocyte containing phagocytosed conidia is indicated in magnified panel (open white arrowhead). Off-target expression of the transgene was also observed in tissues including the somites (full white arrowhead). (B) Treatment with C-001 and C-016 resulted in significantly increased phagocytosis of conidia by GFP/mCherry+ (human FPR1-expressing) neutrophils compared to GFP(only) wild-type cells. Each point represents an infected larva. N ≥ 40 larva scored per condition. Data collated from multiple experiments. Statistics: two-tailed T -test. * p ≤ 0.05, *** p ≤ 0.001.

Techniques Used: Expressing, Staining, Imaging, Infection, Labeling, Fluorescence, Two Tailed Test


Structured Review

Addgene inc human formyl peptide receptor fpr1
Bifunctional compounds enhance phagocytosis of A. fumigatus conidia by zebrafish neutrophils expressing human <t>FPR1.</t> (A) (i) Diagram of experimental approach: Calcofluor-stained A. fumigatus conidia (blue) are co-delivered with treatments into the circulation via the Duct of Cuvier at 3 days post-fertilization. Imaging is performed at 2 h post-infection at a distal site, the caudal venous plexus, which is rich in leukocytes. (ii) Example image of 3 dpf irf8 -MO treated Tg( mpx :EGFP) larva (GFP-labeled neutrophils) with mosaic expression of human FPR1 (traced by mCherry, red fluorescence), 2 h following delivery of calcofluor-labeled (blue fluorescent) A. fumigatus conidia. A GFP/mCherry co-labeled leukocyte containing phagocytosed conidia is indicated in magnified panel (open white arrowhead). Off-target expression of the transgene was also observed in tissues including the somites (full white arrowhead). (B) Treatment with C-001 and C-016 resulted in significantly increased phagocytosis of conidia by GFP/mCherry+ (human FPR1-expressing) neutrophils compared to GFP(only) wild-type cells. Each point represents an infected larva. N ≥ 40 larva scored per condition. Data collated from multiple experiments. Statistics: two-tailed T -test. * p ≤ 0.05, *** p ≤ 0.001.
Human Formyl Peptide Receptor Fpr1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human formyl peptide receptor fpr1/product/Addgene inc
Average 90 stars, based on 1 article reviews
Price from $9.99 to $1999.99
human formyl peptide receptor fpr1 - by Bioz Stars, 2024-04
90/100 stars

Images

1) Product Images from "Bifunctional Small Molecules Enhance Neutrophil Activities Against Aspergillus fumigatus in vivo and in vitro"

Article Title: Bifunctional Small Molecules Enhance Neutrophil Activities Against Aspergillus fumigatus in vivo and in vitro

Journal: Frontiers in Immunology

doi: 10.3389/fimmu.2019.00644

Bifunctional compounds enhance phagocytosis of A. fumigatus conidia by zebrafish neutrophils expressing human FPR1. (A) (i) Diagram of experimental approach: Calcofluor-stained A. fumigatus conidia (blue) are co-delivered with treatments into the circulation via the Duct of Cuvier at 3 days post-fertilization. Imaging is performed at 2 h post-infection at a distal site, the caudal venous plexus, which is rich in leukocytes. (ii) Example image of 3 dpf irf8 -MO treated Tg( mpx :EGFP) larva (GFP-labeled neutrophils) with mosaic expression of human FPR1 (traced by mCherry, red fluorescence), 2 h following delivery of calcofluor-labeled (blue fluorescent) A. fumigatus conidia. A GFP/mCherry co-labeled leukocyte containing phagocytosed conidia is indicated in magnified panel (open white arrowhead). Off-target expression of the transgene was also observed in tissues including the somites (full white arrowhead). (B) Treatment with C-001 and C-016 resulted in significantly increased phagocytosis of conidia by GFP/mCherry+ (human FPR1-expressing) neutrophils compared to GFP(only) wild-type cells. Each point represents an infected larva. N ≥ 40 larva scored per condition. Data collated from multiple experiments. Statistics: two-tailed T -test. * p ≤ 0.05, *** p ≤ 0.001.
Figure Legend Snippet: Bifunctional compounds enhance phagocytosis of A. fumigatus conidia by zebrafish neutrophils expressing human FPR1. (A) (i) Diagram of experimental approach: Calcofluor-stained A. fumigatus conidia (blue) are co-delivered with treatments into the circulation via the Duct of Cuvier at 3 days post-fertilization. Imaging is performed at 2 h post-infection at a distal site, the caudal venous plexus, which is rich in leukocytes. (ii) Example image of 3 dpf irf8 -MO treated Tg( mpx :EGFP) larva (GFP-labeled neutrophils) with mosaic expression of human FPR1 (traced by mCherry, red fluorescence), 2 h following delivery of calcofluor-labeled (blue fluorescent) A. fumigatus conidia. A GFP/mCherry co-labeled leukocyte containing phagocytosed conidia is indicated in magnified panel (open white arrowhead). Off-target expression of the transgene was also observed in tissues including the somites (full white arrowhead). (B) Treatment with C-001 and C-016 resulted in significantly increased phagocytosis of conidia by GFP/mCherry+ (human FPR1-expressing) neutrophils compared to GFP(only) wild-type cells. Each point represents an infected larva. N ≥ 40 larva scored per condition. Data collated from multiple experiments. Statistics: two-tailed T -test. * p ≤ 0.05, *** p ≤ 0.001.

Techniques Used: Expressing, Staining, Imaging, Infection, Labeling, Fluorescence, Two Tailed Test

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    Addgene inc pbgsa fpr1 egfp
    Bifunctional compounds enhance phagocytosis of A. fumigatus conidia by zebrafish neutrophils expressing human <t>FPR1.</t> (A) (i) Diagram of experimental approach: Calcofluor-stained A. fumigatus conidia (blue) are co-delivered with treatments into the circulation via the Duct of Cuvier at 3 days post-fertilization. Imaging is performed at 2 h post-infection at a distal site, the caudal venous plexus, which is rich in leukocytes. (ii) Example image of 3 dpf irf8 -MO treated Tg( mpx <t>:EGFP)</t> larva (GFP-labeled neutrophils) with mosaic expression of human FPR1 (traced by mCherry, red fluorescence), 2 h following delivery of calcofluor-labeled (blue fluorescent) A. fumigatus conidia. A GFP/mCherry co-labeled leukocyte containing phagocytosed conidia is indicated in magnified panel (open white arrowhead). Off-target expression of the transgene was also observed in tissues including the somites (full white arrowhead). (B) Treatment with C-001 and C-016 resulted in significantly increased phagocytosis of conidia by GFP/mCherry+ (human FPR1-expressing) neutrophils compared to GFP(only) wild-type cells. Each point represents an infected larva. N ≥ 40 larva scored per condition. Data collated from multiple experiments. Statistics: two-tailed T -test. * p ≤ 0.05, *** p ≤ 0.001.
    Pbgsa Fpr1 Egfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbgsa fpr1 egfp/product/Addgene inc
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pbgsa fpr1 egfp - by Bioz Stars, 2024-04
    90/100 stars
      Buy from Supplier

    90
    Addgene inc human formyl peptide receptor fpr1
    Bifunctional compounds enhance phagocytosis of A. fumigatus conidia by zebrafish neutrophils expressing human <t>FPR1.</t> (A) (i) Diagram of experimental approach: Calcofluor-stained A. fumigatus conidia (blue) are co-delivered with treatments into the circulation via the Duct of Cuvier at 3 days post-fertilization. Imaging is performed at 2 h post-infection at a distal site, the caudal venous plexus, which is rich in leukocytes. (ii) Example image of 3 dpf irf8 -MO treated Tg( mpx :EGFP) larva (GFP-labeled neutrophils) with mosaic expression of human FPR1 (traced by mCherry, red fluorescence), 2 h following delivery of calcofluor-labeled (blue fluorescent) A. fumigatus conidia. A GFP/mCherry co-labeled leukocyte containing phagocytosed conidia is indicated in magnified panel (open white arrowhead). Off-target expression of the transgene was also observed in tissues including the somites (full white arrowhead). (B) Treatment with C-001 and C-016 resulted in significantly increased phagocytosis of conidia by GFP/mCherry+ (human FPR1-expressing) neutrophils compared to GFP(only) wild-type cells. Each point represents an infected larva. N ≥ 40 larva scored per condition. Data collated from multiple experiments. Statistics: two-tailed T -test. * p ≤ 0.05, *** p ≤ 0.001.
    Human Formyl Peptide Receptor Fpr1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human formyl peptide receptor fpr1/product/Addgene inc
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human formyl peptide receptor fpr1 - by Bioz Stars, 2024-04
    90/100 stars
      Buy from Supplier

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    Bifunctional compounds enhance phagocytosis of A. fumigatus conidia by zebrafish neutrophils expressing human FPR1. (A) (i) Diagram of experimental approach: Calcofluor-stained A. fumigatus conidia (blue) are co-delivered with treatments into the circulation via the Duct of Cuvier at 3 days post-fertilization. Imaging is performed at 2 h post-infection at a distal site, the caudal venous plexus, which is rich in leukocytes. (ii) Example image of 3 dpf irf8 -MO treated Tg( mpx :EGFP) larva (GFP-labeled neutrophils) with mosaic expression of human FPR1 (traced by mCherry, red fluorescence), 2 h following delivery of calcofluor-labeled (blue fluorescent) A. fumigatus conidia. A GFP/mCherry co-labeled leukocyte containing phagocytosed conidia is indicated in magnified panel (open white arrowhead). Off-target expression of the transgene was also observed in tissues including the somites (full white arrowhead). (B) Treatment with C-001 and C-016 resulted in significantly increased phagocytosis of conidia by GFP/mCherry+ (human FPR1-expressing) neutrophils compared to GFP(only) wild-type cells. Each point represents an infected larva. N ≥ 40 larva scored per condition. Data collated from multiple experiments. Statistics: two-tailed T -test. * p ≤ 0.05, *** p ≤ 0.001.

    Journal: Frontiers in Immunology

    Article Title: Bifunctional Small Molecules Enhance Neutrophil Activities Against Aspergillus fumigatus in vivo and in vitro

    doi: 10.3389/fimmu.2019.00644

    Figure Lengend Snippet: Bifunctional compounds enhance phagocytosis of A. fumigatus conidia by zebrafish neutrophils expressing human FPR1. (A) (i) Diagram of experimental approach: Calcofluor-stained A. fumigatus conidia (blue) are co-delivered with treatments into the circulation via the Duct of Cuvier at 3 days post-fertilization. Imaging is performed at 2 h post-infection at a distal site, the caudal venous plexus, which is rich in leukocytes. (ii) Example image of 3 dpf irf8 -MO treated Tg( mpx :EGFP) larva (GFP-labeled neutrophils) with mosaic expression of human FPR1 (traced by mCherry, red fluorescence), 2 h following delivery of calcofluor-labeled (blue fluorescent) A. fumigatus conidia. A GFP/mCherry co-labeled leukocyte containing phagocytosed conidia is indicated in magnified panel (open white arrowhead). Off-target expression of the transgene was also observed in tissues including the somites (full white arrowhead). (B) Treatment with C-001 and C-016 resulted in significantly increased phagocytosis of conidia by GFP/mCherry+ (human FPR1-expressing) neutrophils compared to GFP(only) wild-type cells. Each point represents an infected larva. N ≥ 40 larva scored per condition. Data collated from multiple experiments. Statistics: two-tailed T -test. * p ≤ 0.05, *** p ≤ 0.001.

    Article Snippet: Human formyl-peptide receptor (FPR1) was sub-cloned from pBGSA FPR1-EGFP (Addgene ID:62604) into a middle entry vector and combined with existing 5′ (lyzC promoter) and 3′ (T2A-mCherry) vectors using standard Gateway approaches.

    Techniques: Expressing, Staining, Imaging, Infection, Labeling, Fluorescence, Two Tailed Test

    Bifunctional compounds enhance phagocytosis of A. fumigatus conidia by zebrafish neutrophils expressing human FPR1. (A) (i) Diagram of experimental approach: Calcofluor-stained A. fumigatus conidia (blue) are co-delivered with treatments into the circulation via the Duct of Cuvier at 3 days post-fertilization. Imaging is performed at 2 h post-infection at a distal site, the caudal venous plexus, which is rich in leukocytes. (ii) Example image of 3 dpf irf8 -MO treated Tg( mpx :EGFP) larva (GFP-labeled neutrophils) with mosaic expression of human FPR1 (traced by mCherry, red fluorescence), 2 h following delivery of calcofluor-labeled (blue fluorescent) A. fumigatus conidia. A GFP/mCherry co-labeled leukocyte containing phagocytosed conidia is indicated in magnified panel (open white arrowhead). Off-target expression of the transgene was also observed in tissues including the somites (full white arrowhead). (B) Treatment with C-001 and C-016 resulted in significantly increased phagocytosis of conidia by GFP/mCherry+ (human FPR1-expressing) neutrophils compared to GFP(only) wild-type cells. Each point represents an infected larva. N ≥ 40 larva scored per condition. Data collated from multiple experiments. Statistics: two-tailed T -test. * p ≤ 0.05, *** p ≤ 0.001.

    Journal: Frontiers in Immunology

    Article Title: Bifunctional Small Molecules Enhance Neutrophil Activities Against Aspergillus fumigatus in vivo and in vitro

    doi: 10.3389/fimmu.2019.00644

    Figure Lengend Snippet: Bifunctional compounds enhance phagocytosis of A. fumigatus conidia by zebrafish neutrophils expressing human FPR1. (A) (i) Diagram of experimental approach: Calcofluor-stained A. fumigatus conidia (blue) are co-delivered with treatments into the circulation via the Duct of Cuvier at 3 days post-fertilization. Imaging is performed at 2 h post-infection at a distal site, the caudal venous plexus, which is rich in leukocytes. (ii) Example image of 3 dpf irf8 -MO treated Tg( mpx :EGFP) larva (GFP-labeled neutrophils) with mosaic expression of human FPR1 (traced by mCherry, red fluorescence), 2 h following delivery of calcofluor-labeled (blue fluorescent) A. fumigatus conidia. A GFP/mCherry co-labeled leukocyte containing phagocytosed conidia is indicated in magnified panel (open white arrowhead). Off-target expression of the transgene was also observed in tissues including the somites (full white arrowhead). (B) Treatment with C-001 and C-016 resulted in significantly increased phagocytosis of conidia by GFP/mCherry+ (human FPR1-expressing) neutrophils compared to GFP(only) wild-type cells. Each point represents an infected larva. N ≥ 40 larva scored per condition. Data collated from multiple experiments. Statistics: two-tailed T -test. * p ≤ 0.05, *** p ≤ 0.001.

    Article Snippet: Human formyl-peptide receptor (FPR1) was sub-cloned from pBGSA FPR1-EGFP (Addgene ID:62604) into a middle entry vector and combined with existing 5′ (lyzC promoter) and 3′ (T2A-mCherry) vectors using standard Gateway approaches.

    Techniques: Expressing, Staining, Imaging, Infection, Labeling, Fluorescence, Two Tailed Test