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Promega pax2 1 expression vector
Expression of fadd is down-regulated in noi mutant zebrafish. ( A–F ) fadd expression in wild-type (wt), <t>pax2.1</t> −/− coloboma mutant ( noi ) and lamb1 −/− coloboma mutant ( gup ). Gene expression tested at either 24 (24
Pax2 1 Expression Vector, supplied by Promega, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 85 stars, based on 1 article reviews
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pax2 1 expression vector - by Bioz Stars, 2020-05
85/100 stars

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Article Title: Pax2 regulates a fadd-dependent molecular switch that drives tissue fusion during eye development

Journal: Human Molecular Genetics

doi: 10.1093/hmg/dds056

Expression of fadd is down-regulated in noi mutant zebrafish. ( A–F ) fadd expression in wild-type (wt), pax2.1 −/− coloboma mutant ( noi ) and lamb1 −/− coloboma mutant ( gup ). Gene expression tested at either 24 (24
Figure Legend Snippet: Expression of fadd is down-regulated in noi mutant zebrafish. ( A–F ) fadd expression in wild-type (wt), pax2.1 −/− coloboma mutant ( noi ) and lamb1 −/− coloboma mutant ( gup ). Gene expression tested at either 24 (24

Techniques Used: Expressing, Mutagenesis

Rescue of the pax2.1 -deficient phenotype by mRNA injection. ( A – E ) Wild-type embryos (wt) were injected with pax2.1 mRNA. ( F – J ) Uninjected pax2.1 -deficient (mt) embryos ( K – O ) pax2.1 -deficient embryos injected with pax2.1 mRNA. (
Figure Legend Snippet: Rescue of the pax2.1 -deficient phenotype by mRNA injection. ( A – E ) Wild-type embryos (wt) were injected with pax2.1 mRNA. ( F – J ) Uninjected pax2.1 -deficient (mt) embryos ( K – O ) pax2.1 -deficient embryos injected with pax2.1 mRNA. (

Techniques Used: Injection

Pax2.1 binds to the fadd promoter. ( A ) EMSA using fadd oligonucleotide probe containing the second pax2 -binding site. Fadd probe bound to the pax2.1 protein (lane 2), which was displaced by unlabelled competitor (lane 3) and in the presence of pax2 antibody
Figure Legend Snippet: Pax2.1 binds to the fadd promoter. ( A ) EMSA using fadd oligonucleotide probe containing the second pax2 -binding site. Fadd probe bound to the pax2.1 protein (lane 2), which was displaced by unlabelled competitor (lane 3) and in the presence of pax2 antibody

Techniques Used: Binding Assay

pax2.1 responsive elements in the fadd promoter. ( A ) Diagram at top depicts symbols used for transcription factor-binding sites. p, pax2.1 site; v, vax2 site; rxr, rxr site. Hatched box, exon 1 of fadd . L, luciferase gene. Reporter constructs transfected
Figure Legend Snippet: pax2.1 responsive elements in the fadd promoter. ( A ) Diagram at top depicts symbols used for transcription factor-binding sites. p, pax2.1 site; v, vax2 site; rxr, rxr site. Hatched box, exon 1 of fadd . L, luciferase gene. Reporter constructs transfected

Techniques Used: Binding Assay, Luciferase, Construct, Transfection

Spatiotemporal RIP3 localization and rescue of eye phenotype using necrostatin-1. ( A – C ) RIP3 immunohistochemistry (red) in wild-type (wt) eyes counterstained with DAPI. ( D and E ) High-level RIP3 labelling in pax2.1 -deficient eyes (mt). White arrow
Figure Legend Snippet: Spatiotemporal RIP3 localization and rescue of eye phenotype using necrostatin-1. ( A – C ) RIP3 immunohistochemistry (red) in wild-type (wt) eyes counterstained with DAPI. ( D and E ) High-level RIP3 labelling in pax2.1 -deficient eyes (mt). White arrow

Techniques Used: Immunohistochemistry

Related Articles

Expressing:

Article Title: Pax2 regulates a fadd-dependent molecular switch that drives tissue fusion during eye development
Article Snippet: .. Full-length zebrafish pax2.1 protein was synthesized by in vitro translation from a pax2.1 expression vector (pGEM-T Easy) using the TNT T7-coupled reticulocyte lysate system (Promega). .. The translated protein was verified using the Transcend™ non-radioactive translation detection system (Promega) according to the manufacturer's instructions.

In Vitro:

Article Title: Pax2 regulates a fadd-dependent molecular switch that drives tissue fusion during eye development
Article Snippet: .. Full-length zebrafish pax2.1 protein was synthesized by in vitro translation from a pax2.1 expression vector (pGEM-T Easy) using the TNT T7-coupled reticulocyte lysate system (Promega). .. The translated protein was verified using the Transcend™ non-radioactive translation detection system (Promega) according to the manufacturer's instructions.

Plasmid Preparation:

Article Title: Pax2 regulates a fadd-dependent molecular switch that drives tissue fusion during eye development
Article Snippet: .. Full-length zebrafish pax2.1 protein was synthesized by in vitro translation from a pax2.1 expression vector (pGEM-T Easy) using the TNT T7-coupled reticulocyte lysate system (Promega). .. The translated protein was verified using the Transcend™ non-radioactive translation detection system (Promega) according to the manufacturer's instructions.

Synthesized:

Article Title: Pax2 regulates a fadd-dependent molecular switch that drives tissue fusion during eye development
Article Snippet: .. Full-length zebrafish pax2.1 protein was synthesized by in vitro translation from a pax2.1 expression vector (pGEM-T Easy) using the TNT T7-coupled reticulocyte lysate system (Promega). .. The translated protein was verified using the Transcend™ non-radioactive translation detection system (Promega) according to the manufacturer's instructions.

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    Promega pax2 1 expression vector
    Expression of fadd is down-regulated in noi mutant zebrafish. ( A–F ) fadd expression in wild-type (wt), <t>pax2.1</t> −/− coloboma mutant ( noi ) and lamb1 −/− coloboma mutant ( gup ). Gene expression tested at either 24 (24
    Pax2 1 Expression Vector, supplied by Promega, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pax2 1 expression vector/product/Promega
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pax2 1 expression vector - by Bioz Stars, 2020-05
    85/100 stars
      Buy from Supplier

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    Expression of fadd is down-regulated in noi mutant zebrafish. ( A–F ) fadd expression in wild-type (wt), pax2.1 −/− coloboma mutant ( noi ) and lamb1 −/− coloboma mutant ( gup ). Gene expression tested at either 24 (24

    Journal: Human Molecular Genetics

    Article Title: Pax2 regulates a fadd-dependent molecular switch that drives tissue fusion during eye development

    doi: 10.1093/hmg/dds056

    Figure Lengend Snippet: Expression of fadd is down-regulated in noi mutant zebrafish. ( A–F ) fadd expression in wild-type (wt), pax2.1 −/− coloboma mutant ( noi ) and lamb1 −/− coloboma mutant ( gup ). Gene expression tested at either 24 (24

    Article Snippet: Full-length zebrafish pax2.1 protein was synthesized by in vitro translation from a pax2.1 expression vector (pGEM-T Easy) using the TNT T7-coupled reticulocyte lysate system (Promega).

    Techniques: Expressing, Mutagenesis

    Rescue of the pax2.1 -deficient phenotype by mRNA injection. ( A – E ) Wild-type embryos (wt) were injected with pax2.1 mRNA. ( F – J ) Uninjected pax2.1 -deficient (mt) embryos ( K – O ) pax2.1 -deficient embryos injected with pax2.1 mRNA. (

    Journal: Human Molecular Genetics

    Article Title: Pax2 regulates a fadd-dependent molecular switch that drives tissue fusion during eye development

    doi: 10.1093/hmg/dds056

    Figure Lengend Snippet: Rescue of the pax2.1 -deficient phenotype by mRNA injection. ( A – E ) Wild-type embryos (wt) were injected with pax2.1 mRNA. ( F – J ) Uninjected pax2.1 -deficient (mt) embryos ( K – O ) pax2.1 -deficient embryos injected with pax2.1 mRNA. (

    Article Snippet: Full-length zebrafish pax2.1 protein was synthesized by in vitro translation from a pax2.1 expression vector (pGEM-T Easy) using the TNT T7-coupled reticulocyte lysate system (Promega).

    Techniques: Injection

    Pax2.1 binds to the fadd promoter. ( A ) EMSA using fadd oligonucleotide probe containing the second pax2 -binding site. Fadd probe bound to the pax2.1 protein (lane 2), which was displaced by unlabelled competitor (lane 3) and in the presence of pax2 antibody

    Journal: Human Molecular Genetics

    Article Title: Pax2 regulates a fadd-dependent molecular switch that drives tissue fusion during eye development

    doi: 10.1093/hmg/dds056

    Figure Lengend Snippet: Pax2.1 binds to the fadd promoter. ( A ) EMSA using fadd oligonucleotide probe containing the second pax2 -binding site. Fadd probe bound to the pax2.1 protein (lane 2), which was displaced by unlabelled competitor (lane 3) and in the presence of pax2 antibody

    Article Snippet: Full-length zebrafish pax2.1 protein was synthesized by in vitro translation from a pax2.1 expression vector (pGEM-T Easy) using the TNT T7-coupled reticulocyte lysate system (Promega).

    Techniques: Binding Assay

    pax2.1 responsive elements in the fadd promoter. ( A ) Diagram at top depicts symbols used for transcription factor-binding sites. p, pax2.1 site; v, vax2 site; rxr, rxr site. Hatched box, exon 1 of fadd . L, luciferase gene. Reporter constructs transfected

    Journal: Human Molecular Genetics

    Article Title: Pax2 regulates a fadd-dependent molecular switch that drives tissue fusion during eye development

    doi: 10.1093/hmg/dds056

    Figure Lengend Snippet: pax2.1 responsive elements in the fadd promoter. ( A ) Diagram at top depicts symbols used for transcription factor-binding sites. p, pax2.1 site; v, vax2 site; rxr, rxr site. Hatched box, exon 1 of fadd . L, luciferase gene. Reporter constructs transfected

    Article Snippet: Full-length zebrafish pax2.1 protein was synthesized by in vitro translation from a pax2.1 expression vector (pGEM-T Easy) using the TNT T7-coupled reticulocyte lysate system (Promega).

    Techniques: Binding Assay, Luciferase, Construct, Transfection

    Spatiotemporal RIP3 localization and rescue of eye phenotype using necrostatin-1. ( A – C ) RIP3 immunohistochemistry (red) in wild-type (wt) eyes counterstained with DAPI. ( D and E ) High-level RIP3 labelling in pax2.1 -deficient eyes (mt). White arrow

    Journal: Human Molecular Genetics

    Article Title: Pax2 regulates a fadd-dependent molecular switch that drives tissue fusion during eye development

    doi: 10.1093/hmg/dds056

    Figure Lengend Snippet: Spatiotemporal RIP3 localization and rescue of eye phenotype using necrostatin-1. ( A – C ) RIP3 immunohistochemistry (red) in wild-type (wt) eyes counterstained with DAPI. ( D and E ) High-level RIP3 labelling in pax2.1 -deficient eyes (mt). White arrow

    Article Snippet: Full-length zebrafish pax2.1 protein was synthesized by in vitro translation from a pax2.1 expression vector (pGEM-T Easy) using the TNT T7-coupled reticulocyte lysate system (Promega).

    Techniques: Immunohistochemistry