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Cell Signaling Technology Inc pathscan sandwich elisa assay
LiCl enhances inhibitory GSK-3β phosphorylation in human RMS cell lines. <t>PathScan</t> ® <t>ELISA</t> assays detecting total and phospho-GSK-3β (serine 9) were performed after 36 h of single or combined treatment with 1 μM ATO and 25 mM LiCl in three RMS cell lines in quadruplicate. Mock treated control was set to 100% GSK-3β abundance, respectively phosphorylation. The relative phosphorylation was calculated by normalizing GSK-3β phosphorylation to total GSK-3β. Error bars indicate the standard deviation. Significant differences between groups are indicated by small letters. Multiplicity adjusted p values for each treatment against mock treated control were determined: “a” indicates p ≤ 0.0001, “b” indicates p ≤ 0.05, “c” indicates p ≤ 0.01. In addition, p values for combined treatment relative to single treatment were determined: “d” indicates p ≤ 0.0001.
Pathscan Sandwich Elisa Assay, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Combined application of arsenic trioxide and lithium chloride augments viability reduction and apoptosis induction in human rhabdomyosarcoma cell lines"

Article Title: Combined application of arsenic trioxide and lithium chloride augments viability reduction and apoptosis induction in human rhabdomyosarcoma cell lines

Journal: PLoS ONE

doi: 10.1371/journal.pone.0178857

LiCl enhances inhibitory GSK-3β phosphorylation in human RMS cell lines. PathScan ® ELISA assays detecting total and phospho-GSK-3β (serine 9) were performed after 36 h of single or combined treatment with 1 μM ATO and 25 mM LiCl in three RMS cell lines in quadruplicate. Mock treated control was set to 100% GSK-3β abundance, respectively phosphorylation. The relative phosphorylation was calculated by normalizing GSK-3β phosphorylation to total GSK-3β. Error bars indicate the standard deviation. Significant differences between groups are indicated by small letters. Multiplicity adjusted p values for each treatment against mock treated control were determined: “a” indicates p ≤ 0.0001, “b” indicates p ≤ 0.05, “c” indicates p ≤ 0.01. In addition, p values for combined treatment relative to single treatment were determined: “d” indicates p ≤ 0.0001.
Figure Legend Snippet: LiCl enhances inhibitory GSK-3β phosphorylation in human RMS cell lines. PathScan ® ELISA assays detecting total and phospho-GSK-3β (serine 9) were performed after 36 h of single or combined treatment with 1 μM ATO and 25 mM LiCl in three RMS cell lines in quadruplicate. Mock treated control was set to 100% GSK-3β abundance, respectively phosphorylation. The relative phosphorylation was calculated by normalizing GSK-3β phosphorylation to total GSK-3β. Error bars indicate the standard deviation. Significant differences between groups are indicated by small letters. Multiplicity adjusted p values for each treatment against mock treated control were determined: “a” indicates p ≤ 0.0001, “b” indicates p ≤ 0.05, “c” indicates p ≤ 0.01. In addition, p values for combined treatment relative to single treatment were determined: “d” indicates p ≤ 0.0001.

Techniques Used: Enzyme-linked Immunosorbent Assay, Standard Deviation

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Incubation:

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Caspase-3 Activity Assay:

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Transfection:

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    TBMS1 inhibits the phosphorylation of <t>AKT</t> and NF-κB <t>p65</t> in LPS-exposed BV-2 cells. The BV-2 cells were pretreated with TBMS1 (1, 2 and 4 μM) for 1 h, then incubated with LPS (1 μg/mL) for 1 h. ( A ) The protein levels of AKT, NF-κB p65 and their phosphorylated forms were tested by western blotting. The phosphorylation of AKT ( B ) and NF-κB p65 ( C ) was analyzed relative to β-actin. The results are shown as mean ± SD of three independent experiments. # p
    Akt, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TBMS1 inhibits the phosphorylation of AKT and NF-κB p65 in LPS-exposed BV-2 cells. The BV-2 cells were pretreated with TBMS1 (1, 2 and 4 μM) for 1 h, then incubated with LPS (1 μg/mL) for 1 h. ( A ) The protein levels of AKT, NF-κB p65 and their phosphorylated forms were tested by western blotting. The phosphorylation of AKT ( B ) and NF-κB p65 ( C ) was analyzed relative to β-actin. The results are shown as mean ± SD of three independent experiments. # p

    Journal: International Journal of Molecular Sciences

    Article Title: Tubeimoside I Protects Dopaminergic Neurons Against Inflammation-Mediated Damage in Lipopolysaccharide (LPS)-Evoked Model of Parkinson’s Disease in Rats

    doi: 10.3390/ijms19082242

    Figure Lengend Snippet: TBMS1 inhibits the phosphorylation of AKT and NF-κB p65 in LPS-exposed BV-2 cells. The BV-2 cells were pretreated with TBMS1 (1, 2 and 4 μM) for 1 h, then incubated with LPS (1 μg/mL) for 1 h. ( A ) The protein levels of AKT, NF-κB p65 and their phosphorylated forms were tested by western blotting. The phosphorylation of AKT ( B ) and NF-κB p65 ( C ) was analyzed relative to β-actin. The results are shown as mean ± SD of three independent experiments. # p

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    Techniques: Incubation, Western Blot

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    Journal: Stem Cell Research & Therapy

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    doi: 10.1186/s13287-017-0756-2

    Figure Lengend Snippet: The effect of human umbilical cord mesenchymal stem cell (huMSC) transplantation on neuronal apoptosis of the cortex and hippocampus. a Cell apoptosis was evaluated by H E staining. The boxes indicate neurons arranged loosely and neuron loss. The arrows indicate nucleus shrinkage. Relative number of surviving neurons in b the cortex and c the hippocampus. d Representative cropped Western blots and statistical analysis of the apoptosis-related proteins Bcl-xl, Bax, cleaved caspase-3 (CCaspase-3), and cleaved poly-ADP-ribose polymerase (CPARP) in the cortex and hippocampus. All data are expressed as mean ± SEM, n = 3. Data were generated from three independent experiments. * P

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    Techniques: Transplantation Assay, Staining, Western Blot, Generated

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    Journal: mBio

    Article Title: Community Development between Porphyromonas gingivalis and Candida albicans Mediated by InlJ and Als3

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    Figure Lengend Snippet: C. albicans Als3 interacts with InlJ of P. gingivalis . (A) Attachment of rInlJ protein to S. cerevisiae cells expressing candidal adhesins Als3sm, Als3lg, or Cwp1, or empty pBC542 vector was analyzed with an ELISA using His-tagged MAb (1:2,000). (B) Attachment of the P. gingivalis ( Pg ) WT strain, the Δ inlJ mutant, or the cΔ inlJ mutant to S. cerevisiae cells expressing candidal adhesins Als3lg or Cwp1, or empty pBC542 vector was analyzed with an ELISA using P. gingivalis antibodies (1:5,000). Results are representative of 3 independent experiments and are expressed as means ± SD; n = 3. **, P

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    Techniques: Expressing, Plasmid Preparation, Enzyme-linked Immunosorbent Assay, Mutagenesis

    Effects of tylotoin on MAPK signaling pathways. ( A–D ) Western blot showed effects of cathelicidin-NV on ERK, JNK and p38 protein kinase phosphorylation ( A ) and relative activation analysis ( B – D ). The results were quantified by ImageJ. The densitometry of phosphorylated ERK, JNK and p38 were normalized to total ERK, JNK and p38, and graphed as the mean ± SEM ( n = 5). The values with treatment by cathelicidin-NV are significantly different from the values for the control (** P

    Journal: Biochemical Journal

    Article Title: A frog cathelicidin peptide effectively promotes cutaneous wound healing in mice

    doi: 10.1042/BCJ20180286

    Figure Lengend Snippet: Effects of tylotoin on MAPK signaling pathways. ( A–D ) Western blot showed effects of cathelicidin-NV on ERK, JNK and p38 protein kinase phosphorylation ( A ) and relative activation analysis ( B – D ). The results were quantified by ImageJ. The densitometry of phosphorylated ERK, JNK and p38 were normalized to total ERK, JNK and p38, and graphed as the mean ± SEM ( n = 5). The values with treatment by cathelicidin-NV are significantly different from the values for the control (** P

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    Techniques: Western Blot, Activation Assay