pathscan phospho trkb elisa kit  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc pathscan phospho trkb elisa kit
    Pathscan Phospho Trkb Elisa Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    pathscan phospho trkb elisa kit  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc pathscan phospho trkb elisa kit
    Effect of gintonin on the activation of <t>TrkB</t> receptors in IAA-treated HT22 cells. ( A ) Representative microscopy images of TrkB phosphorylation. HT22 cells were exposed to IAA (5 μM, 2 h) and treated with gintonin (3 μg/mL, 24 h) or BDNF (30 ng/mL, 24 h). <t>Phosphorylated</t> <t>TrkB</t> is dyed green. Microscopy images were captured at the same magnification, scale bar = 100 μm. ( B ) Quantitative analysis of the images obtained from fluorescence microscopy. ( C ) The level of phosphorylation of TrkB receptors measured using a phosphor-TrkB <t>ELISA</t> kit. The data are expressed as the mean ± standard error of the mean (SEM; n = 4). # p < 0.05 and ## p < 0.01 compared to the control group; ** p < 0.01 compared to the IAA-treated group.
    Pathscan Phospho Trkb Elisa Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Protective Effects of Gintonin on Reactive Oxygen Species-Induced HT22 Cell Damages: Involvement of LPA1 Receptor-BDNF-AKT Signaling Pathway"

    Article Title: Protective Effects of Gintonin on Reactive Oxygen Species-Induced HT22 Cell Damages: Involvement of LPA1 Receptor-BDNF-AKT Signaling Pathway

    Journal: Molecules

    doi: 10.3390/molecules26144138

    Effect of gintonin on the activation of TrkB receptors in IAA-treated HT22 cells. ( A ) Representative microscopy images of TrkB phosphorylation. HT22 cells were exposed to IAA (5 μM, 2 h) and treated with gintonin (3 μg/mL, 24 h) or BDNF (30 ng/mL, 24 h). Phosphorylated TrkB is dyed green. Microscopy images were captured at the same magnification, scale bar = 100 μm. ( B ) Quantitative analysis of the images obtained from fluorescence microscopy. ( C ) The level of phosphorylation of TrkB receptors measured using a phosphor-TrkB ELISA kit. The data are expressed as the mean ± standard error of the mean (SEM; n = 4). # p < 0.05 and ## p < 0.01 compared to the control group; ** p < 0.01 compared to the IAA-treated group.
    Figure Legend Snippet: Effect of gintonin on the activation of TrkB receptors in IAA-treated HT22 cells. ( A ) Representative microscopy images of TrkB phosphorylation. HT22 cells were exposed to IAA (5 μM, 2 h) and treated with gintonin (3 μg/mL, 24 h) or BDNF (30 ng/mL, 24 h). Phosphorylated TrkB is dyed green. Microscopy images were captured at the same magnification, scale bar = 100 μm. ( B ) Quantitative analysis of the images obtained from fluorescence microscopy. ( C ) The level of phosphorylation of TrkB receptors measured using a phosphor-TrkB ELISA kit. The data are expressed as the mean ± standard error of the mean (SEM; n = 4). # p < 0.05 and ## p < 0.01 compared to the control group; ** p < 0.01 compared to the IAA-treated group.

    Techniques Used: Activation Assay, Microscopy, Fluorescence, Enzyme-linked Immunosorbent Assay

    Effect of gintonin on Akt phosphorylation in the presence of IAA in HT22 cells. ( A ) Representative microscopy images of Akt phosphorylation. The phosphorylation of Akt was detected using immunochemistry staining. HT22 cells were treated with IAA (5 μM, 2 h), followed by exposure to gintonin (3 μg/mL, 24 h). Phosphorylated TrkB is dyed green, and nuclei are dyed blue using DAPI. Microscopic images were captured at the same magnification, scale bar = 100 μm. ( B ) Quantitative analysis of the images obtained from fluorescence microscopy. ( C ) The expression of p-Akt and t-Akt proteins was detected by Western blot analysis using β-actin as a loading control. ( D ) p-Akt/t-Akt ratio at each dose. The data are represented as the mean ± SEM ( n = 4). # p < 0.05 and ## p < 0.01 compared to control group; * p < 0.05 and ** p < 0.01 compared to the IAA-treated group.
    Figure Legend Snippet: Effect of gintonin on Akt phosphorylation in the presence of IAA in HT22 cells. ( A ) Representative microscopy images of Akt phosphorylation. The phosphorylation of Akt was detected using immunochemistry staining. HT22 cells were treated with IAA (5 μM, 2 h), followed by exposure to gintonin (3 μg/mL, 24 h). Phosphorylated TrkB is dyed green, and nuclei are dyed blue using DAPI. Microscopic images were captured at the same magnification, scale bar = 100 μm. ( B ) Quantitative analysis of the images obtained from fluorescence microscopy. ( C ) The expression of p-Akt and t-Akt proteins was detected by Western blot analysis using β-actin as a loading control. ( D ) p-Akt/t-Akt ratio at each dose. The data are represented as the mean ± SEM ( n = 4). # p < 0.05 and ## p < 0.01 compared to control group; * p < 0.05 and ** p < 0.01 compared to the IAA-treated group.

    Techniques Used: Microscopy, Staining, Fluorescence, Expressing, Western Blot

    bovine serum albumin  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc bovine serum albumin
    Bovine Serum Albumin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    pathscan phospho trkb pantyr sandwich elisa  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc pathscan phospho trkb pantyr sandwich elisa
    Pathscan Phospho Trkb Pantyr Sandwich Elisa, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    107 t3 3 equation  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc 107 t3 3 equation
    107 T3 3 Equation, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    elisa test pathscan phospho trkb pantyr  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc elisa test pathscan phospho trkb pantyr
    Elisa Test Pathscan Phospho Trkb Pantyr, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    pathscan phospho trkb tyr516 sandwich elisa kit  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc pathscan phospho trkb tyr516 sandwich elisa kit
    Pathscan Phospho Trkb Tyr516 Sandwich Elisa Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pathscan phospho trkb tyr516 sandwich elisa kit/product/Cell Signaling Technology Inc
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    phospho trkb brain levels  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phospho trkb brain levels
    (A–D) Expression of p75NTR and <t>TrkB</t> in prefrontal cortex of CTR and STZ rats with or without ed‐NGF treatment, analyzed by Western blot, is shown in A and B, respectively. The figure on upper side shows the representative Western blots for the two NGF receptors. C reports the graph of TrkB and p75NTR ratio in the PFC of the different rat groups. The <t>p‐TrkB</t> levels were measured by ELISA, and results are shown in D. Statistically significant changes: a P < 0.05 b P < 0.01 versus CTR; c P < 0.05 and d P < 0.01 versus CTR + NGF; e P < 0.05 and versus STZ.
    Phospho Trkb Brain Levels, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Ocular Nerve Growth Factor Administration Modulates Brain‐derived Neurotrophic Factor Signaling in Prefrontal Cortex of Healthy and Diabetic Rats"

    Article Title: Ocular Nerve Growth Factor Administration Modulates Brain‐derived Neurotrophic Factor Signaling in Prefrontal Cortex of Healthy and Diabetic Rats

    Journal: CNS Neuroscience & Therapeutics

    doi: 10.1111/cns.12661

    (A–D) Expression of p75NTR and TrkB in prefrontal cortex of CTR and STZ rats with or without ed‐NGF treatment, analyzed by Western blot, is shown in A and B, respectively. The figure on upper side shows the representative Western blots for the two NGF receptors. C reports the graph of TrkB and p75NTR ratio in the PFC of the different rat groups. The p‐TrkB levels were measured by ELISA, and results are shown in D. Statistically significant changes: a P < 0.05 b P < 0.01 versus CTR; c P < 0.05 and d P < 0.01 versus CTR + NGF; e P < 0.05 and versus STZ.
    Figure Legend Snippet: (A–D) Expression of p75NTR and TrkB in prefrontal cortex of CTR and STZ rats with or without ed‐NGF treatment, analyzed by Western blot, is shown in A and B, respectively. The figure on upper side shows the representative Western blots for the two NGF receptors. C reports the graph of TrkB and p75NTR ratio in the PFC of the different rat groups. The p‐TrkB levels were measured by ELISA, and results are shown in D. Statistically significant changes: a P < 0.05 b P < 0.01 versus CTR; c P < 0.05 and d P < 0.01 versus CTR + NGF; e P < 0.05 and versus STZ.

    Techniques Used: Expressing, Western Blot, Enzyme-linked Immunosorbent Assay

    (A–C) Correlation between FST immobilization time and the PFC expression of proBDNF (A), p‐TrkB levels (B), and PSA‐NCAM (C). Both p‐TrkB levels and PSA‐NCAM are positively correlated with the FST immobilization time (P < 0.01), while a negative correlation was found for proBDNF (P = 0.0054).
    Figure Legend Snippet: (A–C) Correlation between FST immobilization time and the PFC expression of proBDNF (A), p‐TrkB levels (B), and PSA‐NCAM (C). Both p‐TrkB levels and PSA‐NCAM are positively correlated with the FST immobilization time (P < 0.01), while a negative correlation was found for proBDNF (P = 0.0054).

    Techniques Used: Expressing

    pathscan phospho trkb tyr516 sandwich elisa kit  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc pathscan phospho trkb tyr516 sandwich elisa kit
    Pathscan Phospho Trkb Tyr516 Sandwich Elisa Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pathscan phospho trkb tyr516 sandwich elisa kit/product/Cell Signaling Technology Inc
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    phospho trkb brain levels  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phospho trkb brain levels
    (A–D) Expression of p75NTR and <t>TrkB</t> in prefrontal cortex of CTR and STZ rats with or without ed‐NGF treatment, analyzed by Western blot, is shown in A and B, respectively. The figure on upper side shows the representative Western blots for the two NGF receptors. C reports the graph of TrkB and p75NTR ratio in the PFC of the different rat groups. The <t>p‐TrkB</t> levels were measured by ELISA, and results are shown in D. Statistically significant changes: a P < 0.05 b P < 0.01 versus CTR; c P < 0.05 and d P < 0.01 versus CTR + NGF; e P < 0.05 and versus STZ.
    Phospho Trkb Brain Levels, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho trkb brain levels/product/Cell Signaling Technology Inc
    Average 91 stars, based on 1 article reviews
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    phospho trkb brain levels - by Bioz Stars, 2024-07
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    Images

    1) Product Images from "Ocular Nerve Growth Factor Administration Modulates Brain‐derived Neurotrophic Factor Signaling in Prefrontal Cortex of Healthy and Diabetic Rats"

    Article Title: Ocular Nerve Growth Factor Administration Modulates Brain‐derived Neurotrophic Factor Signaling in Prefrontal Cortex of Healthy and Diabetic Rats

    Journal: CNS Neuroscience & Therapeutics

    doi: 10.1111/cns.12661

    (A–D) Expression of p75NTR and TrkB in prefrontal cortex of CTR and STZ rats with or without ed‐NGF treatment, analyzed by Western blot, is shown in A and B, respectively. The figure on upper side shows the representative Western blots for the two NGF receptors. C reports the graph of TrkB and p75NTR ratio in the PFC of the different rat groups. The p‐TrkB levels were measured by ELISA, and results are shown in D. Statistically significant changes: a P < 0.05 b P < 0.01 versus CTR; c P < 0.05 and d P < 0.01 versus CTR + NGF; e P < 0.05 and versus STZ.
    Figure Legend Snippet: (A–D) Expression of p75NTR and TrkB in prefrontal cortex of CTR and STZ rats with or without ed‐NGF treatment, analyzed by Western blot, is shown in A and B, respectively. The figure on upper side shows the representative Western blots for the two NGF receptors. C reports the graph of TrkB and p75NTR ratio in the PFC of the different rat groups. The p‐TrkB levels were measured by ELISA, and results are shown in D. Statistically significant changes: a P < 0.05 b P < 0.01 versus CTR; c P < 0.05 and d P < 0.01 versus CTR + NGF; e P < 0.05 and versus STZ.

    Techniques Used: Expressing, Western Blot, Enzyme-linked Immunosorbent Assay

    (A–C) Correlation between FST immobilization time and the PFC expression of proBDNF (A), p‐TrkB levels (B), and PSA‐NCAM (C). Both p‐TrkB levels and PSA‐NCAM are positively correlated with the FST immobilization time (P < 0.01), while a negative correlation was found for proBDNF (P = 0.0054).
    Figure Legend Snippet: (A–C) Correlation between FST immobilization time and the PFC expression of proBDNF (A), p‐TrkB levels (B), and PSA‐NCAM (C). Both p‐TrkB levels and PSA‐NCAM are positively correlated with the FST immobilization time (P < 0.01), while a negative correlation was found for proBDNF (P = 0.0054).

    Techniques Used: Expressing

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    Cell Signaling Technology Inc pathscan phospho trkb elisa kit
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    Pathscan Phospho Trkb Tyr516 Sandwich Elisa Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc phospho trkb brain levels
    (A–D) Expression of p75NTR and <t>TrkB</t> in prefrontal cortex of CTR and STZ rats with or without ed‐NGF treatment, analyzed by Western blot, is shown in A and B, respectively. The figure on upper side shows the representative Western blots for the two NGF receptors. C reports the graph of TrkB and p75NTR ratio in the PFC of the different rat groups. The <t>p‐TrkB</t> levels were measured by ELISA, and results are shown in D. Statistically significant changes: a P < 0.05 b P < 0.01 versus CTR; c P < 0.05 and d P < 0.01 versus CTR + NGF; e P < 0.05 and versus STZ.
    Phospho Trkb Brain Levels, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    (A–D) Expression of p75NTR and TrkB in prefrontal cortex of CTR and STZ rats with or without ed‐NGF treatment, analyzed by Western blot, is shown in A and B, respectively. The figure on upper side shows the representative Western blots for the two NGF receptors. C reports the graph of TrkB and p75NTR ratio in the PFC of the different rat groups. The p‐TrkB levels were measured by ELISA, and results are shown in D. Statistically significant changes: a P < 0.05 b P < 0.01 versus CTR; c P < 0.05 and d P < 0.01 versus CTR + NGF; e P < 0.05 and versus STZ.

    Journal: CNS Neuroscience & Therapeutics

    Article Title: Ocular Nerve Growth Factor Administration Modulates Brain‐derived Neurotrophic Factor Signaling in Prefrontal Cortex of Healthy and Diabetic Rats

    doi: 10.1111/cns.12661

    Figure Lengend Snippet: (A–D) Expression of p75NTR and TrkB in prefrontal cortex of CTR and STZ rats with or without ed‐NGF treatment, analyzed by Western blot, is shown in A and B, respectively. The figure on upper side shows the representative Western blots for the two NGF receptors. C reports the graph of TrkB and p75NTR ratio in the PFC of the different rat groups. The p‐TrkB levels were measured by ELISA, and results are shown in D. Statistically significant changes: a P < 0.05 b P < 0.01 versus CTR; c P < 0.05 and d P < 0.01 versus CTR + NGF; e P < 0.05 and versus STZ.

    Article Snippet: The phospho‐TrkB brain levels were measured using PathScan Phospho‐TrkB (Tyr516) Sandwich ELISA Kit (#7111C, Cell Signaling Technology, Inc) following the protocol supplied by the manufacturer.

    Techniques: Expressing, Western Blot, Enzyme-linked Immunosorbent Assay

    (A–C) Correlation between FST immobilization time and the PFC expression of proBDNF (A), p‐TrkB levels (B), and PSA‐NCAM (C). Both p‐TrkB levels and PSA‐NCAM are positively correlated with the FST immobilization time (P < 0.01), while a negative correlation was found for proBDNF (P = 0.0054).

    Journal: CNS Neuroscience & Therapeutics

    Article Title: Ocular Nerve Growth Factor Administration Modulates Brain‐derived Neurotrophic Factor Signaling in Prefrontal Cortex of Healthy and Diabetic Rats

    doi: 10.1111/cns.12661

    Figure Lengend Snippet: (A–C) Correlation between FST immobilization time and the PFC expression of proBDNF (A), p‐TrkB levels (B), and PSA‐NCAM (C). Both p‐TrkB levels and PSA‐NCAM are positively correlated with the FST immobilization time (P < 0.01), while a negative correlation was found for proBDNF (P = 0.0054).

    Article Snippet: The phospho‐TrkB brain levels were measured using PathScan Phospho‐TrkB (Tyr516) Sandwich ELISA Kit (#7111C, Cell Signaling Technology, Inc) following the protocol supplied by the manufacturer.

    Techniques: Expressing