Journal: PLoS ONE

Article Title: Mesenchymal Stem Cells Secrete Multiple Cytokines That Promote Angiogenesis and Have Contrasting Effects on Chemotaxis and Apoptosis

doi: 10.1371/journal.pone.0035685

Figure Lengend Snippet: Changes in caspase-3, phospho-Akt (Ser473), phospho-Akt (Thr308) and phospho-Bad (Ser112) in H9c2 cells treated with Mesencult (Mes) or conditioned media (CM) for 24 hours under hypoxic conditions. Data calculated as a mean percent of Mesencult (control) treated cultures ± SE. a = p<0.05, b = p<0.01 compared to controls.

Article Snippet: An ELISA was used to detect changes in the levels of total-Bad, phospho-Bad (Ser112), total-Akt, phospho-Akt (Ser473), phospho-Akt (Thr308) and ERK 1/2 using PathScan Sandwich ELISA Kits (Cell Signaling Technology #7162, 7182, 7170, 7160, 7252) or a STAR ERK 1/2 ELISA kit (Upstate #17-463).

Techniques:

Journal: PLoS ONE

Article Title: Mesenchymal Stem Cells Secrete Multiple Cytokines That Promote Angiogenesis and Have Contrasting Effects on Chemotaxis and Apoptosis

doi: 10.1371/journal.pone.0035685

Figure Lengend Snippet: Changes in caspase-3, phospho-Akt (Ser473), phospho-Akt (Thr308) and phospho-Bad (Ser112) in H9c2 cells treated with Mesencult (Mes), MCP-1 or MCP-1 + PI 3-Kγ inhibitor for 24 hours under hypoxic conditions. Data calculated as a percent of Mesencult (control) treated cultures ± SE. a = p<0.05, b = p<0.01 compared to controls.

Article Snippet: An ELISA was used to detect changes in the levels of total-Bad, phospho-Bad (Ser112), total-Akt, phospho-Akt (Ser473), phospho-Akt (Thr308) and ERK 1/2 using PathScan Sandwich ELISA Kits (Cell Signaling Technology #7162, 7182, 7170, 7160, 7252) or a STAR ERK 1/2 ELISA kit (Upstate #17-463).

Techniques:

Journal: PLoS ONE

Article Title: Mesenchymal Stem Cells Secrete Multiple Cytokines That Promote Angiogenesis and Have Contrasting Effects on Chemotaxis and Apoptosis

doi: 10.1371/journal.pone.0035685

Figure Lengend Snippet: Changes in phospho-Akt (Ser473), phospho-Akt (Thr308) and phospho-Bad (Ser112) in H9c2 cells treated with Mesencult (Mes), conditioned media (CM) or ERK 1/2 inhibitor for 6 hours under hypoxic conditions. Data calculated as a percent of Mesencult (control) treated cultures ± SE. a = p<0.05, b = p<0.01 compared to controls.

Article Snippet: An ELISA was used to detect changes in the levels of total-Bad, phospho-Bad (Ser112), total-Akt, phospho-Akt (Ser473), phospho-Akt (Thr308) and ERK 1/2 using PathScan Sandwich ELISA Kits (Cell Signaling Technology #7162, 7182, 7170, 7160, 7252) or a STAR ERK 1/2 ELISA kit (Upstate #17-463).

Techniques:

Journal: Comparative Hepatology

Article Title: Regenerative and fibrotic pathways in canine hepatic portosystemic shunt and portal vein hypoplasia, new models for clinical hepatocyte growth factor treatment

doi: 10.1186/1476-5926-4-7

Figure Lengend Snippet: Used antibodies in Western blot experiments.

Article Snippet: p-PKB (Thr 308) , 60 , 1:1,000 , Cell-Signaling , Anti-mouse HRP , 1:20,000.

Techniques: Western Blot

Journal: PLoS ONE

Article Title: A Ser252Trp Mutation in Fibroblast Growth Factor Receptor 2 (FGFR2) Mimicking Human Apert Syndrome Reveals an Essential Role for FGF Signaling in the Regulation of Endochondral Bone Formation

doi: 10.1371/journal.pone.0087311

Figure Lengend Snippet: (A) Western blotting demonstrated that levels of phospho-Erk1/2 and phospho-p38 were both increased in cultured BMSCs from MT mice. There was, however, no change in phospho-AKT. (B, C) The p38 and Erk1/2 pathways were inhibited by SB203580 and PD98059, respectively. (D–G) Relative expression of genes in BMSCs treated with SB203580 or PD98059 for 21 days. The expression levels of Col2 , Col10 , OC , and OP were significantly increased in both WT and MT BMSCs treated with SB203580; however, only OC and OP were increased after PD98059 treatment. (Student's t-test, * P <0.05, ** P <0.01 versus untreated BMSCs, # P <0.05 versus wild-type BMSCs.). (H) Inhibition of p38 and Erk1/2 pathway by SB203580 and PD98059 rescued the growth retardation in cultured mutant tibia bones. The white double-headed arrows represent the total length (TL) and ossified tissue length (OL). (I) Percentage increases in TL and OL of phalange bones after culture for 7 days. (Student's t-test, # P<0.05 versus wild-type *P<0.05 versus mutant type.)

Article Snippet: Blotted protein was probed with antibodies specific to phospho-p38 and p38, phospho-Erk1/2 and Erk1/2, phospho-AKT, and AKT (Cell Signaling Technology, USA) as described previously .

Techniques: Western Blot, Cell Culture, Expressing, Inhibition, Mutagenesis

Journal: iScience

Article Title: Betacellulin promotes tumor development and EGFR mutant lung cancer growth by stimulating the EGFR pathway and suppressing apoptosis

doi: 10.1016/j.isci.2022.104211

Figure Lengend Snippet:

Article Snippet: Akt , Cell Signaling Technology , Cat# 9272S RRID: AB_329827.

Techniques: Neutralization, Microarray, Mutagenesis, Recombinant, Transfection, Staining, Plasmid Preparation, shRNA, Software