pasteurella multocida  (Millipore)


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  • 94
    Name:
    Pasteurella multocida toxin
    Description:

    Catalog Number:
    p5806
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    Structured Review

    Millipore pasteurella multocida

    https://www.bioz.com/result/pasteurella multocida/product/Millipore
    Average 94 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    pasteurella multocida - by Bioz Stars, 2020-09
    94/100 stars

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    Incubation:

    Article Title: Mutations in the H7 HA and PB1 genes of avian influenza a viruses increase viral pathogenicity and contact transmission in guinea pigs
    Article Snippet: .. Resialylation with α2,3-linked SAs was performed by incubation at 37°C for 2 h with 6 mU of α2,3-sialyltransferase from Pasteurella multocida (Sigma-Aldrich), whereas resialylation with α2,6-linked SA was achieved by incubation with 38 mU α2,6-sialyltransferase from Photobacterium damsela (Sigma-Aldrich). .. To generate α2,3-linked SA TRBCs and α2,6-linked SA TRBCs, 1.5 mM cytidine-5′-monophospho-N-acetylneuraminic acid sodium salt (CMP, Sigma-Aldrich) was used.

    Recombinant:

    Article Title: Identification of non-substrate-like glycosyltransferase inhibitors from library screening: pitfalls & hits of non-substrate-like glycosyltransferase inhibitors from library screening: pitfalls & hits †Electronic supplementary information (ESI) available. See DOI: 10.1039/c7md00550d
    Article Snippet: .. Recombinant α-(2,3)-sialyltransferase from Pasteurella multocida was purchased from Sigma. .. Recombinant human fucosyltransferase 2 (FUT2) was purchased from R & D Systems.

    Purification:

    Article Title: The two-pore channel TPC1 is required for efficient protein processing through early and recycling endosomes
    Article Snippet: .. Cholera toxin (CT) and diphtheria toxin (DT) were obtained from Sigma; Pasteurella multocida toxin (PMT) was purified as described in ref. and anthrax protective antigen and lethal factor as in ref. . .. Phospho-ERK antibody (Phospho-p44/42 MAPK (Thr202/Tyr204)) was obtained from Cell Signaling (mAb #4370) and anti-GFP antibody from Abcam (#6658).

    Synthesized:

    Article Title: Plasticity of Amino Acid Residue 145 Near the Receptor Binding Site of H3 Swine Influenza A Viruses and Its Impact on Receptor Binding and Antibody Recognition
    Article Snippet: .. Monospecific preparations of Fet-HRP were synthesized using α-2,3-sialyltransferase from Pasteurella multocida (Sigma, St. Louis, MO) for 3-modified fetuin (3-Fet-HRP) or α-2,6-sialyltransferase from Photobacterium damsela (Sigma, St. Louis, MO) for 6-modified fetuin (6-Fet-HRP), essentially as described previously ( ). .. Ninety-six-well native fetuin-coated flat-bottom plates (Greiner Bio-One, Monroe, NC) were incubated overnight at 4°C with 128 HAU of each virus in 0.02 M Tris-buffered saline (TBS), pH 7.2 to 7.4.

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  • 99
    Millipore spectinomycin
    ICE Mh1 PM22 fitness costs, addiction, and consequences for antimicrobial resistance in transconjugants. (A) Growth curves of P. multocida CCUG 17976 (spontaneous rifampin-resistant mutant) and isogenic ICE Mh1 PM22 transconjugant. Mean of 4 biological replicates with SEM. (B) Growth curves of M. haemolytica ATCC 33396 (spontaneous rifampin-resistant mutant) and isogenic ICE Mh1 PM22 transconjugant. Mean of 4 biological replicates with SEM. (C) Luciferase-based competition index from co-cultures of ICE Mh1 PM22 transconjugants and E. coli DH5α harboring the luciferase expression plasmid pAKlux2. Mean of 4 biological replicates with SEM; t-test, ∗ P ≤ 0.05. (D) Long-term repeated passage of ICE Mh1 PM22 transconjugants. Transconjugants were grown in MH (no drug) or MH + 0.5 MIC (subinhibitory; MIC for susceptible P. multocida CCUG 17976 or M. haemolytica ATCC 33396 WT) for 150 days and plated on media with or without selective concentrations of indicated antimicrobials (i.e., 0.5 MIC for non-susceptible isogenic transconjugants). Mean of 3 biological replicates with SEM. (E) Upper panel: growth curves of co-cultures of E. coli DH5α pAKlux2 and E. coli DH5α, P. multocida CCUG 17976 ICE Mh1 PM22 , or M. haemolytica ATCC 33396 ICE Mh1 PM22 in subinhibitory (0.5 MIC for susceptible P. multocida CCUG 17976 or M. haemolytica ATCC 33396) concentrations of oxytetracycline (left) or <t>spectinomycin</t> (right). Mean of 3 biological replicates with SEM. Lower panel: detection of E. coli luciferase production in co-cultures (as above) with either oxytetracycline (left) or spectinomycin (right). (F) Schematic of checkerboard synergy assay for drug interaction screening.
    Spectinomycin, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 70 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/spectinomycin/product/Millipore
    Average 99 stars, based on 70 article reviews
    Price from $9.99 to $1999.99
    spectinomycin - by Bioz Stars, 2020-09
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    ICE Mh1 PM22 fitness costs, addiction, and consequences for antimicrobial resistance in transconjugants. (A) Growth curves of P. multocida CCUG 17976 (spontaneous rifampin-resistant mutant) and isogenic ICE Mh1 PM22 transconjugant. Mean of 4 biological replicates with SEM. (B) Growth curves of M. haemolytica ATCC 33396 (spontaneous rifampin-resistant mutant) and isogenic ICE Mh1 PM22 transconjugant. Mean of 4 biological replicates with SEM. (C) Luciferase-based competition index from co-cultures of ICE Mh1 PM22 transconjugants and E. coli DH5α harboring the luciferase expression plasmid pAKlux2. Mean of 4 biological replicates with SEM; t-test, ∗ P ≤ 0.05. (D) Long-term repeated passage of ICE Mh1 PM22 transconjugants. Transconjugants were grown in MH (no drug) or MH + 0.5 MIC (subinhibitory; MIC for susceptible P. multocida CCUG 17976 or M. haemolytica ATCC 33396 WT) for 150 days and plated on media with or without selective concentrations of indicated antimicrobials (i.e., 0.5 MIC for non-susceptible isogenic transconjugants). Mean of 3 biological replicates with SEM. (E) Upper panel: growth curves of co-cultures of E. coli DH5α pAKlux2 and E. coli DH5α, P. multocida CCUG 17976 ICE Mh1 PM22 , or M. haemolytica ATCC 33396 ICE Mh1 PM22 in subinhibitory (0.5 MIC for susceptible P. multocida CCUG 17976 or M. haemolytica ATCC 33396) concentrations of oxytetracycline (left) or spectinomycin (right). Mean of 3 biological replicates with SEM. Lower panel: detection of E. coli luciferase production in co-cultures (as above) with either oxytetracycline (left) or spectinomycin (right). (F) Schematic of checkerboard synergy assay for drug interaction screening.

    Journal: Frontiers in Microbiology

    Article Title: Emerging Variants of the Integrative and Conjugant Element ICEMh1 in Livestock Pathogens: Structural Insights, Potential Host Range, and Implications for Bacterial Fitness and Antimicrobial Therapy

    doi: 10.3389/fmicb.2019.02608

    Figure Lengend Snippet: ICE Mh1 PM22 fitness costs, addiction, and consequences for antimicrobial resistance in transconjugants. (A) Growth curves of P. multocida CCUG 17976 (spontaneous rifampin-resistant mutant) and isogenic ICE Mh1 PM22 transconjugant. Mean of 4 biological replicates with SEM. (B) Growth curves of M. haemolytica ATCC 33396 (spontaneous rifampin-resistant mutant) and isogenic ICE Mh1 PM22 transconjugant. Mean of 4 biological replicates with SEM. (C) Luciferase-based competition index from co-cultures of ICE Mh1 PM22 transconjugants and E. coli DH5α harboring the luciferase expression plasmid pAKlux2. Mean of 4 biological replicates with SEM; t-test, ∗ P ≤ 0.05. (D) Long-term repeated passage of ICE Mh1 PM22 transconjugants. Transconjugants were grown in MH (no drug) or MH + 0.5 MIC (subinhibitory; MIC for susceptible P. multocida CCUG 17976 or M. haemolytica ATCC 33396 WT) for 150 days and plated on media with or without selective concentrations of indicated antimicrobials (i.e., 0.5 MIC for non-susceptible isogenic transconjugants). Mean of 3 biological replicates with SEM. (E) Upper panel: growth curves of co-cultures of E. coli DH5α pAKlux2 and E. coli DH5α, P. multocida CCUG 17976 ICE Mh1 PM22 , or M. haemolytica ATCC 33396 ICE Mh1 PM22 in subinhibitory (0.5 MIC for susceptible P. multocida CCUG 17976 or M. haemolytica ATCC 33396) concentrations of oxytetracycline (left) or spectinomycin (right). Mean of 3 biological replicates with SEM. Lower panel: detection of E. coli luciferase production in co-cultures (as above) with either oxytetracycline (left) or spectinomycin (right). (F) Schematic of checkerboard synergy assay for drug interaction screening.

    Article Snippet: Briefly, in three independent experiments, OD600 1.0 equivalents of P. multocida PM22 and RifR P. multocida CCUG 17976 or M. haemolytica ATCC 33396 were mated in 100 μL of BHI broth spotted on TSA blood agar, incubated aerobically at 37°C for 4 h, harvested and then plated on BHI agar supplemented with rifampin (50 mg/L), tetracycline (10 mg/L; MilliporeSigma), and spectinomycin (100 mg/L; MilliporeSigma).

    Techniques: Mutagenesis, Luciferase, Expressing, Plasmid Preparation