chitosan plga plga pvpon alk γ pga plga  (ATCC)


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    ATCC chitosan plga plga pvpon alk γ pga plga
    Types of nanoparticles studied for antigen delivery in parasites and other microorganisms.
    Chitosan Plga Plga Pvpon Alk γ Pga Plga, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Conserved Candidate Antigens and Nanoparticles to Develop Vaccine against Giardia intestinalis"

    Article Title: Conserved Candidate Antigens and Nanoparticles to Develop Vaccine against Giardia intestinalis

    Journal: Vaccines

    doi: 10.3390/vaccines11010096

    Types of nanoparticles studied for antigen delivery in parasites and other microorganisms.
    Figure Legend Snippet: Types of nanoparticles studied for antigen delivery in parasites and other microorganisms.

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    chitosan plga plga pvpon alk γ pga plga  (ATCC)


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    ATCC chitosan plga plga pvpon alk γ pga plga
    Types of nanoparticles studied for antigen delivery in parasites and other microorganisms.
    Chitosan Plga Plga Pvpon Alk γ Pga Plga, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Conserved Candidate Antigens and Nanoparticles to Develop Vaccine against Giardia intestinalis"

    Article Title: Conserved Candidate Antigens and Nanoparticles to Develop Vaccine against Giardia intestinalis

    Journal: Vaccines

    doi: 10.3390/vaccines11010096

    Types of nanoparticles studied for antigen delivery in parasites and other microorganisms.
    Figure Legend Snippet: Types of nanoparticles studied for antigen delivery in parasites and other microorganisms.

    Techniques Used:

    c albicans nprcoe 1601030  (ATCC)


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    ATCC c albicans nprcoe 1601030
    C Albicans Nprcoe 1601030, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ceratocystis paradoxa  (ATCC)


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    ATCC ceratocystis paradoxa
    Pathogenic strains used in this study.
    Ceratocystis Paradoxa, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Discovery of a Novel Lineage Burkholderia cepacia ST 1870 Endophytically Isolated from Medicinal Polygala paniculata Which Shows Potent In Vitro Antileishmanial and Antimicrobial Effects"

    Article Title: Discovery of a Novel Lineage Burkholderia cepacia ST 1870 Endophytically Isolated from Medicinal Polygala paniculata Which Shows Potent In Vitro Antileishmanial and Antimicrobial Effects

    Journal: International Journal of Microbiology

    doi: 10.1155/2021/6618559

    Pathogenic strains used in this study.
    Figure Legend Snippet: Pathogenic strains used in this study.

    Techniques Used:

    Results of the initial screening against pathogens.
    Figure Legend Snippet: Results of the initial screening against pathogens.

    Techniques Used:

    Antimicrobial activity of NPE (a) produced by B. cepacia COPS. Columns (b) and (c) correspond to different crude extracts' antibacterial activity compared to overlay assay (top to bottom: Bacillus cereus , Escherichia coli, and Enterococcus faecalis ). Lanes (d) COPS NPE, (e) benomyl, as positive control, and (f) negative control represent the antifungal effect. From top to bottom: Moniliophthora perniciosa, Ceratocystis paradoxa , Sclerotinia sclerotiorum , Alternaria alternata , and Fusarium verticillioides.
    Figure Legend Snippet: Antimicrobial activity of NPE (a) produced by B. cepacia COPS. Columns (b) and (c) correspond to different crude extracts' antibacterial activity compared to overlay assay (top to bottom: Bacillus cereus , Escherichia coli, and Enterococcus faecalis ). Lanes (d) COPS NPE, (e) benomyl, as positive control, and (f) negative control represent the antifungal effect. From top to bottom: Moniliophthora perniciosa, Ceratocystis paradoxa , Sclerotinia sclerotiorum , Alternaria alternata , and Fusarium verticillioides.

    Techniques Used: Activity Assay, Produced, Overlay Assay, Positive Control, Negative Control

    Antagonistic activity of B. cepacia strain COPS NPE toward phytopathogenic fungi and pathogenic bacteria.
    Figure Legend Snippet: Antagonistic activity of B. cepacia strain COPS NPE toward phytopathogenic fungi and pathogenic bacteria.

    Techniques Used: Activity Assay

    human il 17a f heterodimer  (ATCC)


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    ATCC human il 17a f heterodimer
    In vitro binding parameters of ixekizumab to human, cynomolgus monkey, and rabbit <t> IL-17A </t> determined using surface plasmon resonance
    Human Il 17a F Heterodimer, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Generation and characterization of ixekizumab, a humanized monoclonal antibody that neutralizes interleukin-17A"

    Article Title: Generation and characterization of ixekizumab, a humanized monoclonal antibody that neutralizes interleukin-17A

    Journal: Journal of Inflammation Research

    doi: 10.2147/JIR.S100940

    In vitro binding parameters of ixekizumab to human, cynomolgus monkey, and rabbit  IL-17A  determined using surface plasmon resonance
    Figure Legend Snippet: In vitro binding parameters of ixekizumab to human, cynomolgus monkey, and rabbit IL-17A determined using surface plasmon resonance

    Techniques Used: In Vitro, Binding Assay

    Ixekizumab binds specifically to IL-17A. Notes: Human IL-17 family member proteins, mouse IL-17A, and human IL-22 were coated into individual ELISA plate wells. Ixekizumab was then added at varying concentration up to 10 µg/mL. The absorbance at 450 nm represents the average values and standard error obtained from duplicate determinations at each concentration of ixekizumab bound to the test proteins. Abbreviations: ELISA, enzyme-linked immunosorbent assay; IL, interleukin.
    Figure Legend Snippet: Ixekizumab binds specifically to IL-17A. Notes: Human IL-17 family member proteins, mouse IL-17A, and human IL-22 were coated into individual ELISA plate wells. Ixekizumab was then added at varying concentration up to 10 µg/mL. The absorbance at 450 nm represents the average values and standard error obtained from duplicate determinations at each concentration of ixekizumab bound to the test proteins. Abbreviations: ELISA, enzyme-linked immunosorbent assay; IL, interleukin.

    Techniques Used: Enzyme-linked Immunosorbent Assay, Concentration Assay

    Ixekizumab competes with IL-17RA binding to immobilized human IL-17A. Notes: Human IL-17A was immobilized on a Biacore chip surface. Binding of IL-17RA/Fc to immobilized IL-17A was shown as increase in response units upon injection of IL-17RA/Fc ( A ). Ixekizumab bound to immobilized IL-17A as shown by the increase in response units following injection ( B ). Upon binding of ixekizumab to IL-17A, IL-17RA/Fc can no longer bind ( B ). Abbreviations: IL, interleukin; RU, response units; Resp Diff, the difference in RU between active flow cell and reference flow cell.
    Figure Legend Snippet: Ixekizumab competes with IL-17RA binding to immobilized human IL-17A. Notes: Human IL-17A was immobilized on a Biacore chip surface. Binding of IL-17RA/Fc to immobilized IL-17A was shown as increase in response units upon injection of IL-17RA/Fc ( A ). Ixekizumab bound to immobilized IL-17A as shown by the increase in response units following injection ( B ). Upon binding of ixekizumab to IL-17A, IL-17RA/Fc can no longer bind ( B ). Abbreviations: IL, interleukin; RU, response units; Resp Diff, the difference in RU between active flow cell and reference flow cell.

    Techniques Used: Binding Assay, Injection

    Ixekizumab neutralizes human IL-17A, human IL-17A/F heterodimer, and cynomolgus monkey IL-17A. Notes: ( A ) HT-29 cells were treated with a dose range of human IL-17A or IL-17A/F for ∼48 hours. GROα was measured in the culture medium by ELISA. ( B ) HT-29 cells were treated with a constant amount of either human IL-17A (60 ng/mL =1,875 pM) or human IL-17A/F (1,000 ng/mL =32,573 pM), in the presence of either ixekizumab or control human IgG4 at the indicated concentrations. After ∼48 hours, GROα in the culture media was measured by ELISA. ( C ) HT-29 colon cancer cells were treated with a constant amount of cynomolgus monkey IL-17 (60 ng/mL), in the presence of either ixekizumab or control human IgG4 at the indicated concentrations for 48 hours, and GROα in the culture media measured by ELISA. Results in ( A ) and ( B ) are shown as the mean of triplicate treatments ± SD and are representative of seven independent experiments. Results in ( C ) are shown as the mean of triplicate treatments ± SD and are representative of two independent experiments. Human and cynomolgus monkey IL-17A were tested for neutralization in separate experiments. Abbreviations: cyno, cynomolgus; ELISA, enzyme-linked immunosorbent assay; IL, interleukin; SD, standard deviation; GRO, human growth-regulated oncogene.
    Figure Legend Snippet: Ixekizumab neutralizes human IL-17A, human IL-17A/F heterodimer, and cynomolgus monkey IL-17A. Notes: ( A ) HT-29 cells were treated with a dose range of human IL-17A or IL-17A/F for ∼48 hours. GROα was measured in the culture medium by ELISA. ( B ) HT-29 cells were treated with a constant amount of either human IL-17A (60 ng/mL =1,875 pM) or human IL-17A/F (1,000 ng/mL =32,573 pM), in the presence of either ixekizumab or control human IgG4 at the indicated concentrations. After ∼48 hours, GROα in the culture media was measured by ELISA. ( C ) HT-29 colon cancer cells were treated with a constant amount of cynomolgus monkey IL-17 (60 ng/mL), in the presence of either ixekizumab or control human IgG4 at the indicated concentrations for 48 hours, and GROα in the culture media measured by ELISA. Results in ( A ) and ( B ) are shown as the mean of triplicate treatments ± SD and are representative of seven independent experiments. Results in ( C ) are shown as the mean of triplicate treatments ± SD and are representative of two independent experiments. Human and cynomolgus monkey IL-17A were tested for neutralization in separate experiments. Abbreviations: cyno, cynomolgus; ELISA, enzyme-linked immunosorbent assay; IL, interleukin; SD, standard deviation; GRO, human growth-regulated oncogene.

    Techniques Used: Enzyme-linked Immunosorbent Assay, Neutralization, Standard Deviation

    Ixekizumab epitope. Notes: ( A ) 4T1 cells were treated with a constant amount of human IL-17A wild type, mutant h1, or vector control supernatant in the presence of increasing amounts of ixekizumab (closed symbols) or isotope control antibody (open symbols). After 48 hours, KC in the supernatant was measured by ELISA. Results are shown as the mean of triplicate treatments ± SD and are representative of four independent experiments. ( B ) Structure of the IL-17A:IL-17RA complex (4hsa) with key amino acid residues in the epitope for ixekizumab highlighted. IL-17RA is colored in magenta, and the IL-17A dimer subunits are colored in cyan and green. The key epitope region (DGNVDYH) in IL-17A for ixekizumab is highlighted in yellow or brown in each subunit of the cytokine. This figure was generated using the PyMOL Molecular Graphics System (Version 1.7.0.3; Schrödinger, LLC). Abbreviations: ELISA, enzyme-linked immunosorbent assay; IL, interleukin; SD, standard deviation; KC, keratinocyte chemoattractant.
    Figure Legend Snippet: Ixekizumab epitope. Notes: ( A ) 4T1 cells were treated with a constant amount of human IL-17A wild type, mutant h1, or vector control supernatant in the presence of increasing amounts of ixekizumab (closed symbols) or isotope control antibody (open symbols). After 48 hours, KC in the supernatant was measured by ELISA. Results are shown as the mean of triplicate treatments ± SD and are representative of four independent experiments. ( B ) Structure of the IL-17A:IL-17RA complex (4hsa) with key amino acid residues in the epitope for ixekizumab highlighted. IL-17RA is colored in magenta, and the IL-17A dimer subunits are colored in cyan and green. The key epitope region (DGNVDYH) in IL-17A for ixekizumab is highlighted in yellow or brown in each subunit of the cytokine. This figure was generated using the PyMOL Molecular Graphics System (Version 1.7.0.3; Schrödinger, LLC). Abbreviations: ELISA, enzyme-linked immunosorbent assay; IL, interleukin; SD, standard deviation; KC, keratinocyte chemoattractant.

    Techniques Used: Mutagenesis, Plasmid Preparation, Enzyme-linked Immunosorbent Assay, Generated, Standard Deviation

    Ixekizumab inhibits human IL-17A-induced KC secretion in a mouse PD model. Notes: Human IL-17A (3 µg) was administered to mice SC 1 hour after IV injection of 1 mg/kg, 0.1 mg/kg, 0.01 mg/kg, or 0.001 mg/kg ixekizumab (corresponding to 20 µg, 2 µg, 0.2 µg, and 0.02 µg per mouse, respectively). KC levels were determined by ELISA 2 hours posthuman IL-17A injection. n=5 mice per group. One-way ANOVA was used for the pairwise comparison of KC chemokine levels between treatments. *The unadjusted P -value is 0.0012 comparing the IL-17A +20 µg control mAb group and the IL-17A +20 µg ixekizumab group. The figure represents one experiment of two separate studies. Abbreviations: SC, subcutaneous; ANOVA, analysis of variance; ELISA, enzyme-linked immunosorbent assay; IL, interleukin; IV, intravenous; KC, keratinocyte chemoattractant; mAb, monoclonal antibody; PD, pharmacodynamic.
    Figure Legend Snippet: Ixekizumab inhibits human IL-17A-induced KC secretion in a mouse PD model. Notes: Human IL-17A (3 µg) was administered to mice SC 1 hour after IV injection of 1 mg/kg, 0.1 mg/kg, 0.01 mg/kg, or 0.001 mg/kg ixekizumab (corresponding to 20 µg, 2 µg, 0.2 µg, and 0.02 µg per mouse, respectively). KC levels were determined by ELISA 2 hours posthuman IL-17A injection. n=5 mice per group. One-way ANOVA was used for the pairwise comparison of KC chemokine levels between treatments. *The unadjusted P -value is 0.0012 comparing the IL-17A +20 µg control mAb group and the IL-17A +20 µg ixekizumab group. The figure represents one experiment of two separate studies. Abbreviations: SC, subcutaneous; ANOVA, analysis of variance; ELISA, enzyme-linked immunosorbent assay; IL, interleukin; IV, intravenous; KC, keratinocyte chemoattractant; mAb, monoclonal antibody; PD, pharmacodynamic.

    Techniques Used: IV Injection, Enzyme-linked Immunosorbent Assay, Injection

    r triatomae dsm 44892  (ATCC)


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    ATCC r triatomae dsm 44892
    R Triatomae Dsm 44892, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    v paradoxa leaves  (ATCC)


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    ATCC v paradoxa leaves
    MIC values of ursolic acid against Gram-positive and Gram-negative bacteria.
    V Paradoxa Leaves, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Anti-Planktonic and Anti-Biofilm Properties of Pentacyclic Triterpenes—Asiatic Acid and Ursolic Acid as Promising Antibacterial Future Pharmaceuticals"

    Article Title: Anti-Planktonic and Anti-Biofilm Properties of Pentacyclic Triterpenes—Asiatic Acid and Ursolic Acid as Promising Antibacterial Future Pharmaceuticals

    Journal: Biomolecules

    doi: 10.3390/biom12010098

    MIC values of ursolic acid against Gram-positive and Gram-negative bacteria.
    Figure Legend Snippet: MIC values of ursolic acid against Gram-positive and Gram-negative bacteria.

    Techniques Used: Activity Assay

    trichocoma paradoxa  (ATCC)


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    ATCC trichocoma paradoxa
    Talaromyces spp. nucleotide sequences employed to build a phylogram to locate phylogenetically our strains from honey
    Trichocoma Paradoxa, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Diversity of xerotolerant and xerophilic fungi in honey"

    Article Title: Diversity of xerotolerant and xerophilic fungi in honey

    Journal: IMA Fungus

    doi: 10.1186/s43008-019-0021-7

    Talaromyces spp. nucleotide sequences employed to build a phylogram to locate phylogenetically our strains from honey
    Figure Legend Snippet: Talaromyces spp. nucleotide sequences employed to build a phylogram to locate phylogenetically our strains from honey

    Techniques Used:

    ML phylogenetic tree built using the ITS, BenA , CaM and rpb 2 concatenated dataset for species of the genus Talaromyces . Species of the section Trachyspermi are indicated in a blue background and those of the section Purpurei in yellow. Trichocoma paradoxa CBS 247.57 was chosen as out-group. Support in nodes is indicated above thick branches and is represented by posterior probabilities (BI analysis) of 0.95 and higher and/or bootstrap values (ML analysis) of 70% and higher. Fully supported branched (100% BS /1 PP) are indicated in bold. T = ex-type strain. Alignment length 2265 bp
    Figure Legend Snippet: ML phylogenetic tree built using the ITS, BenA , CaM and rpb 2 concatenated dataset for species of the genus Talaromyces . Species of the section Trachyspermi are indicated in a blue background and those of the section Purpurei in yellow. Trichocoma paradoxa CBS 247.57 was chosen as out-group. Support in nodes is indicated above thick branches and is represented by posterior probabilities (BI analysis) of 0.95 and higher and/or bootstrap values (ML analysis) of 70% and higher. Fully supported branched (100% BS /1 PP) are indicated in bold. T = ex-type strain. Alignment length 2265 bp

    Techniques Used:

    morphology protein family 31 32 os schizopora paradoxa gn  (ATCC)


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    ATCC morphology protein family 31 32 os schizopora paradoxa gn
    Morphology Protein Family 31 32 Os Schizopora Paradoxa Gn, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1 0 ns ns 18 0 1 0  (ATCC)


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    ATCC 1 0 ns ns 18 0 1 0
    1 0 Ns Ns 18 0 1 0, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    t9 2 cb m 39 guillardia theta ccmp2712 toxoplasma gondii gt1 cyanophora paradoxa porphyridium cruentum cyanidioschyzon merolae  (ATCC)


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    ATCC t9 2 cb m 39 guillardia theta ccmp2712 toxoplasma gondii gt1 cyanophora paradoxa porphyridium cruentum cyanidioschyzon merolae
    T9 2 Cb M 39 Guillardia Theta Ccmp2712 Toxoplasma Gondii Gt1 Cyanophora Paradoxa Porphyridium Cruentum Cyanidioschyzon Merolae, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC chitosan plga plga pvpon alk γ pga plga
    Types of nanoparticles studied for antigen delivery in parasites and other microorganisms.
    Chitosan Plga Plga Pvpon Alk γ Pga Plga, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC c albicans nprcoe 1601030
    Types of nanoparticles studied for antigen delivery in parasites and other microorganisms.
    C Albicans Nprcoe 1601030, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC ceratocystis paradoxa
    Pathogenic strains used in this study.
    Ceratocystis Paradoxa, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC human il 17a f heterodimer
    In vitro binding parameters of ixekizumab to human, cynomolgus monkey, and rabbit <t> IL-17A </t> determined using surface plasmon resonance
    Human Il 17a F Heterodimer, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC r triatomae dsm 44892
    In vitro binding parameters of ixekizumab to human, cynomolgus monkey, and rabbit <t> IL-17A </t> determined using surface plasmon resonance
    R Triatomae Dsm 44892, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC v paradoxa leaves
    MIC values of ursolic acid against Gram-positive and Gram-negative bacteria.
    V Paradoxa Leaves, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    ATCC trichocoma paradoxa
    Talaromyces spp. nucleotide sequences employed to build a phylogram to locate phylogenetically our strains from honey
    Trichocoma Paradoxa, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC morphology protein family 31 32 os schizopora paradoxa gn
    Talaromyces spp. nucleotide sequences employed to build a phylogram to locate phylogenetically our strains from honey
    Morphology Protein Family 31 32 Os Schizopora Paradoxa Gn, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC 1 0 ns ns 18 0 1 0
    Talaromyces spp. nucleotide sequences employed to build a phylogram to locate phylogenetically our strains from honey
    1 0 Ns Ns 18 0 1 0, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC t9 2 cb m 39 guillardia theta ccmp2712 toxoplasma gondii gt1 cyanophora paradoxa porphyridium cruentum cyanidioschyzon merolae
    Talaromyces spp. nucleotide sequences employed to build a phylogram to locate phylogenetically our strains from honey
    T9 2 Cb M 39 Guillardia Theta Ccmp2712 Toxoplasma Gondii Gt1 Cyanophora Paradoxa Porphyridium Cruentum Cyanidioschyzon Merolae, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Types of nanoparticles studied for antigen delivery in parasites and other microorganisms.

    Journal: Vaccines

    Article Title: Conserved Candidate Antigens and Nanoparticles to Develop Vaccine against Giardia intestinalis

    doi: 10.3390/vaccines11010096

    Figure Lengend Snippet: Types of nanoparticles studied for antigen delivery in parasites and other microorganisms.

    Article Snippet: Polymeric , Chitosan PLGA PLGA PVPON Alk γ-PGA PLGA , 160–1000 nm , Hepatitis B Ovalbumin Tetanus toxoid Ovalbumin gp120 (HIV-1) Membrane vesicles ( G. intestinalis WB ATCC 50803 and G. intestinalis GS/M ATCC 50581) , [ ] [ ] [ ] [ ] [ ] [ ] .

    Techniques:

    Pathogenic strains used in this study.

    Journal: International Journal of Microbiology

    Article Title: Discovery of a Novel Lineage Burkholderia cepacia ST 1870 Endophytically Isolated from Medicinal Polygala paniculata Which Shows Potent In Vitro Antileishmanial and Antimicrobial Effects

    doi: 10.1155/2021/6618559

    Figure Lengend Snippet: Pathogenic strains used in this study.

    Article Snippet: Micrococcus luteus ATCC 9341 , Ceratocystis paradoxa.

    Techniques:

    Results of the initial screening against pathogens.

    Journal: International Journal of Microbiology

    Article Title: Discovery of a Novel Lineage Burkholderia cepacia ST 1870 Endophytically Isolated from Medicinal Polygala paniculata Which Shows Potent In Vitro Antileishmanial and Antimicrobial Effects

    doi: 10.1155/2021/6618559

    Figure Lengend Snippet: Results of the initial screening against pathogens.

    Article Snippet: Micrococcus luteus ATCC 9341 , Ceratocystis paradoxa.

    Techniques:

    Antimicrobial activity of NPE (a) produced by B. cepacia COPS. Columns (b) and (c) correspond to different crude extracts' antibacterial activity compared to overlay assay (top to bottom: Bacillus cereus , Escherichia coli, and Enterococcus faecalis ). Lanes (d) COPS NPE, (e) benomyl, as positive control, and (f) negative control represent the antifungal effect. From top to bottom: Moniliophthora perniciosa, Ceratocystis paradoxa , Sclerotinia sclerotiorum , Alternaria alternata , and Fusarium verticillioides.

    Journal: International Journal of Microbiology

    Article Title: Discovery of a Novel Lineage Burkholderia cepacia ST 1870 Endophytically Isolated from Medicinal Polygala paniculata Which Shows Potent In Vitro Antileishmanial and Antimicrobial Effects

    doi: 10.1155/2021/6618559

    Figure Lengend Snippet: Antimicrobial activity of NPE (a) produced by B. cepacia COPS. Columns (b) and (c) correspond to different crude extracts' antibacterial activity compared to overlay assay (top to bottom: Bacillus cereus , Escherichia coli, and Enterococcus faecalis ). Lanes (d) COPS NPE, (e) benomyl, as positive control, and (f) negative control represent the antifungal effect. From top to bottom: Moniliophthora perniciosa, Ceratocystis paradoxa , Sclerotinia sclerotiorum , Alternaria alternata , and Fusarium verticillioides.

    Article Snippet: Micrococcus luteus ATCC 9341 , Ceratocystis paradoxa.

    Techniques: Activity Assay, Produced, Overlay Assay, Positive Control, Negative Control

    Antagonistic activity of B. cepacia strain COPS NPE toward phytopathogenic fungi and pathogenic bacteria.

    Journal: International Journal of Microbiology

    Article Title: Discovery of a Novel Lineage Burkholderia cepacia ST 1870 Endophytically Isolated from Medicinal Polygala paniculata Which Shows Potent In Vitro Antileishmanial and Antimicrobial Effects

    doi: 10.1155/2021/6618559

    Figure Lengend Snippet: Antagonistic activity of B. cepacia strain COPS NPE toward phytopathogenic fungi and pathogenic bacteria.

    Article Snippet: Micrococcus luteus ATCC 9341 , Ceratocystis paradoxa.

    Techniques: Activity Assay

    In vitro binding parameters of ixekizumab to human, cynomolgus monkey, and rabbit  IL-17A  determined using surface plasmon resonance

    Journal: Journal of Inflammation Research

    Article Title: Generation and characterization of ixekizumab, a humanized monoclonal antibody that neutralizes interleukin-17A

    doi: 10.2147/JIR.S100940

    Figure Lengend Snippet: In vitro binding parameters of ixekizumab to human, cynomolgus monkey, and rabbit IL-17A determined using surface plasmon resonance

    Article Snippet: HT-29 cells (ATCC #HTB-38) were treated with a constant amount of human IL-17A (60 ng/mL =1,875 pM), cynomolgus monkey IL-17A (60 ng/mL =1,618 pM), or human IL-17A/F heterodimer (1,000 ng/mL =32,573 pM) in the presence of either ixekizumab or control human IgG4 at the indicated concentrations.

    Techniques: In Vitro, Binding Assay

    Ixekizumab binds specifically to IL-17A. Notes: Human IL-17 family member proteins, mouse IL-17A, and human IL-22 were coated into individual ELISA plate wells. Ixekizumab was then added at varying concentration up to 10 µg/mL. The absorbance at 450 nm represents the average values and standard error obtained from duplicate determinations at each concentration of ixekizumab bound to the test proteins. Abbreviations: ELISA, enzyme-linked immunosorbent assay; IL, interleukin.

    Journal: Journal of Inflammation Research

    Article Title: Generation and characterization of ixekizumab, a humanized monoclonal antibody that neutralizes interleukin-17A

    doi: 10.2147/JIR.S100940

    Figure Lengend Snippet: Ixekizumab binds specifically to IL-17A. Notes: Human IL-17 family member proteins, mouse IL-17A, and human IL-22 were coated into individual ELISA plate wells. Ixekizumab was then added at varying concentration up to 10 µg/mL. The absorbance at 450 nm represents the average values and standard error obtained from duplicate determinations at each concentration of ixekizumab bound to the test proteins. Abbreviations: ELISA, enzyme-linked immunosorbent assay; IL, interleukin.

    Article Snippet: HT-29 cells (ATCC #HTB-38) were treated with a constant amount of human IL-17A (60 ng/mL =1,875 pM), cynomolgus monkey IL-17A (60 ng/mL =1,618 pM), or human IL-17A/F heterodimer (1,000 ng/mL =32,573 pM) in the presence of either ixekizumab or control human IgG4 at the indicated concentrations.

    Techniques: Enzyme-linked Immunosorbent Assay, Concentration Assay

    Ixekizumab competes with IL-17RA binding to immobilized human IL-17A. Notes: Human IL-17A was immobilized on a Biacore chip surface. Binding of IL-17RA/Fc to immobilized IL-17A was shown as increase in response units upon injection of IL-17RA/Fc ( A ). Ixekizumab bound to immobilized IL-17A as shown by the increase in response units following injection ( B ). Upon binding of ixekizumab to IL-17A, IL-17RA/Fc can no longer bind ( B ). Abbreviations: IL, interleukin; RU, response units; Resp Diff, the difference in RU between active flow cell and reference flow cell.

    Journal: Journal of Inflammation Research

    Article Title: Generation and characterization of ixekizumab, a humanized monoclonal antibody that neutralizes interleukin-17A

    doi: 10.2147/JIR.S100940

    Figure Lengend Snippet: Ixekizumab competes with IL-17RA binding to immobilized human IL-17A. Notes: Human IL-17A was immobilized on a Biacore chip surface. Binding of IL-17RA/Fc to immobilized IL-17A was shown as increase in response units upon injection of IL-17RA/Fc ( A ). Ixekizumab bound to immobilized IL-17A as shown by the increase in response units following injection ( B ). Upon binding of ixekizumab to IL-17A, IL-17RA/Fc can no longer bind ( B ). Abbreviations: IL, interleukin; RU, response units; Resp Diff, the difference in RU between active flow cell and reference flow cell.

    Article Snippet: HT-29 cells (ATCC #HTB-38) were treated with a constant amount of human IL-17A (60 ng/mL =1,875 pM), cynomolgus monkey IL-17A (60 ng/mL =1,618 pM), or human IL-17A/F heterodimer (1,000 ng/mL =32,573 pM) in the presence of either ixekizumab or control human IgG4 at the indicated concentrations.

    Techniques: Binding Assay, Injection

    Ixekizumab neutralizes human IL-17A, human IL-17A/F heterodimer, and cynomolgus monkey IL-17A. Notes: ( A ) HT-29 cells were treated with a dose range of human IL-17A or IL-17A/F for ∼48 hours. GROα was measured in the culture medium by ELISA. ( B ) HT-29 cells were treated with a constant amount of either human IL-17A (60 ng/mL =1,875 pM) or human IL-17A/F (1,000 ng/mL =32,573 pM), in the presence of either ixekizumab or control human IgG4 at the indicated concentrations. After ∼48 hours, GROα in the culture media was measured by ELISA. ( C ) HT-29 colon cancer cells were treated with a constant amount of cynomolgus monkey IL-17 (60 ng/mL), in the presence of either ixekizumab or control human IgG4 at the indicated concentrations for 48 hours, and GROα in the culture media measured by ELISA. Results in ( A ) and ( B ) are shown as the mean of triplicate treatments ± SD and are representative of seven independent experiments. Results in ( C ) are shown as the mean of triplicate treatments ± SD and are representative of two independent experiments. Human and cynomolgus monkey IL-17A were tested for neutralization in separate experiments. Abbreviations: cyno, cynomolgus; ELISA, enzyme-linked immunosorbent assay; IL, interleukin; SD, standard deviation; GRO, human growth-regulated oncogene.

    Journal: Journal of Inflammation Research

    Article Title: Generation and characterization of ixekizumab, a humanized monoclonal antibody that neutralizes interleukin-17A

    doi: 10.2147/JIR.S100940

    Figure Lengend Snippet: Ixekizumab neutralizes human IL-17A, human IL-17A/F heterodimer, and cynomolgus monkey IL-17A. Notes: ( A ) HT-29 cells were treated with a dose range of human IL-17A or IL-17A/F for ∼48 hours. GROα was measured in the culture medium by ELISA. ( B ) HT-29 cells were treated with a constant amount of either human IL-17A (60 ng/mL =1,875 pM) or human IL-17A/F (1,000 ng/mL =32,573 pM), in the presence of either ixekizumab or control human IgG4 at the indicated concentrations. After ∼48 hours, GROα in the culture media was measured by ELISA. ( C ) HT-29 colon cancer cells were treated with a constant amount of cynomolgus monkey IL-17 (60 ng/mL), in the presence of either ixekizumab or control human IgG4 at the indicated concentrations for 48 hours, and GROα in the culture media measured by ELISA. Results in ( A ) and ( B ) are shown as the mean of triplicate treatments ± SD and are representative of seven independent experiments. Results in ( C ) are shown as the mean of triplicate treatments ± SD and are representative of two independent experiments. Human and cynomolgus monkey IL-17A were tested for neutralization in separate experiments. Abbreviations: cyno, cynomolgus; ELISA, enzyme-linked immunosorbent assay; IL, interleukin; SD, standard deviation; GRO, human growth-regulated oncogene.

    Article Snippet: HT-29 cells (ATCC #HTB-38) were treated with a constant amount of human IL-17A (60 ng/mL =1,875 pM), cynomolgus monkey IL-17A (60 ng/mL =1,618 pM), or human IL-17A/F heterodimer (1,000 ng/mL =32,573 pM) in the presence of either ixekizumab or control human IgG4 at the indicated concentrations.

    Techniques: Enzyme-linked Immunosorbent Assay, Neutralization, Standard Deviation

    Ixekizumab epitope. Notes: ( A ) 4T1 cells were treated with a constant amount of human IL-17A wild type, mutant h1, or vector control supernatant in the presence of increasing amounts of ixekizumab (closed symbols) or isotope control antibody (open symbols). After 48 hours, KC in the supernatant was measured by ELISA. Results are shown as the mean of triplicate treatments ± SD and are representative of four independent experiments. ( B ) Structure of the IL-17A:IL-17RA complex (4hsa) with key amino acid residues in the epitope for ixekizumab highlighted. IL-17RA is colored in magenta, and the IL-17A dimer subunits are colored in cyan and green. The key epitope region (DGNVDYH) in IL-17A for ixekizumab is highlighted in yellow or brown in each subunit of the cytokine. This figure was generated using the PyMOL Molecular Graphics System (Version 1.7.0.3; Schrödinger, LLC). Abbreviations: ELISA, enzyme-linked immunosorbent assay; IL, interleukin; SD, standard deviation; KC, keratinocyte chemoattractant.

    Journal: Journal of Inflammation Research

    Article Title: Generation and characterization of ixekizumab, a humanized monoclonal antibody that neutralizes interleukin-17A

    doi: 10.2147/JIR.S100940

    Figure Lengend Snippet: Ixekizumab epitope. Notes: ( A ) 4T1 cells were treated with a constant amount of human IL-17A wild type, mutant h1, or vector control supernatant in the presence of increasing amounts of ixekizumab (closed symbols) or isotope control antibody (open symbols). After 48 hours, KC in the supernatant was measured by ELISA. Results are shown as the mean of triplicate treatments ± SD and are representative of four independent experiments. ( B ) Structure of the IL-17A:IL-17RA complex (4hsa) with key amino acid residues in the epitope for ixekizumab highlighted. IL-17RA is colored in magenta, and the IL-17A dimer subunits are colored in cyan and green. The key epitope region (DGNVDYH) in IL-17A for ixekizumab is highlighted in yellow or brown in each subunit of the cytokine. This figure was generated using the PyMOL Molecular Graphics System (Version 1.7.0.3; Schrödinger, LLC). Abbreviations: ELISA, enzyme-linked immunosorbent assay; IL, interleukin; SD, standard deviation; KC, keratinocyte chemoattractant.

    Article Snippet: HT-29 cells (ATCC #HTB-38) were treated with a constant amount of human IL-17A (60 ng/mL =1,875 pM), cynomolgus monkey IL-17A (60 ng/mL =1,618 pM), or human IL-17A/F heterodimer (1,000 ng/mL =32,573 pM) in the presence of either ixekizumab or control human IgG4 at the indicated concentrations.

    Techniques: Mutagenesis, Plasmid Preparation, Enzyme-linked Immunosorbent Assay, Generated, Standard Deviation

    Ixekizumab inhibits human IL-17A-induced KC secretion in a mouse PD model. Notes: Human IL-17A (3 µg) was administered to mice SC 1 hour after IV injection of 1 mg/kg, 0.1 mg/kg, 0.01 mg/kg, or 0.001 mg/kg ixekizumab (corresponding to 20 µg, 2 µg, 0.2 µg, and 0.02 µg per mouse, respectively). KC levels were determined by ELISA 2 hours posthuman IL-17A injection. n=5 mice per group. One-way ANOVA was used for the pairwise comparison of KC chemokine levels between treatments. *The unadjusted P -value is 0.0012 comparing the IL-17A +20 µg control mAb group and the IL-17A +20 µg ixekizumab group. The figure represents one experiment of two separate studies. Abbreviations: SC, subcutaneous; ANOVA, analysis of variance; ELISA, enzyme-linked immunosorbent assay; IL, interleukin; IV, intravenous; KC, keratinocyte chemoattractant; mAb, monoclonal antibody; PD, pharmacodynamic.

    Journal: Journal of Inflammation Research

    Article Title: Generation and characterization of ixekizumab, a humanized monoclonal antibody that neutralizes interleukin-17A

    doi: 10.2147/JIR.S100940

    Figure Lengend Snippet: Ixekizumab inhibits human IL-17A-induced KC secretion in a mouse PD model. Notes: Human IL-17A (3 µg) was administered to mice SC 1 hour after IV injection of 1 mg/kg, 0.1 mg/kg, 0.01 mg/kg, or 0.001 mg/kg ixekizumab (corresponding to 20 µg, 2 µg, 0.2 µg, and 0.02 µg per mouse, respectively). KC levels were determined by ELISA 2 hours posthuman IL-17A injection. n=5 mice per group. One-way ANOVA was used for the pairwise comparison of KC chemokine levels between treatments. *The unadjusted P -value is 0.0012 comparing the IL-17A +20 µg control mAb group and the IL-17A +20 µg ixekizumab group. The figure represents one experiment of two separate studies. Abbreviations: SC, subcutaneous; ANOVA, analysis of variance; ELISA, enzyme-linked immunosorbent assay; IL, interleukin; IV, intravenous; KC, keratinocyte chemoattractant; mAb, monoclonal antibody; PD, pharmacodynamic.

    Article Snippet: HT-29 cells (ATCC #HTB-38) were treated with a constant amount of human IL-17A (60 ng/mL =1,875 pM), cynomolgus monkey IL-17A (60 ng/mL =1,618 pM), or human IL-17A/F heterodimer (1,000 ng/mL =32,573 pM) in the presence of either ixekizumab or control human IgG4 at the indicated concentrations.

    Techniques: IV Injection, Enzyme-linked Immunosorbent Assay, Injection

    MIC values of ursolic acid against Gram-positive and Gram-negative bacteria.

    Journal: Biomolecules

    Article Title: Anti-Planktonic and Anti-Biofilm Properties of Pentacyclic Triterpenes—Asiatic Acid and Ursolic Acid as Promising Antibacterial Future Pharmaceuticals

    doi: 10.3390/biom12010098

    Figure Lengend Snippet: MIC values of ursolic acid against Gram-positive and Gram-negative bacteria.

    Article Snippet: UA isolated from V. paradoxa leaves revealed good antimicrobial activity against reference S. aureus ATCC 33591 and clinical MRSA strains [ ].

    Techniques: Activity Assay

    Talaromyces spp. nucleotide sequences employed to build a phylogram to locate phylogenetically our strains from honey

    Journal: IMA Fungus

    Article Title: Diversity of xerotolerant and xerophilic fungi in honey

    doi: 10.1186/s43008-019-0021-7

    Figure Lengend Snippet: Talaromyces spp. nucleotide sequences employed to build a phylogram to locate phylogenetically our strains from honey

    Article Snippet: Cunninghamella bertholletiae (CBS 693.68), Mucor plumbeus (DAOM 220743), Mucor racemosus (ATCC 42647), and Rhizopus oryzae (CBS 112.07 and CBS 130146) were used as outgroup for the LSU tree; Aphanoascus keratinophilus (IMI 319010) for the Myxotrichaceae taxa tree; and Trichocoma paradoxa (CBS 247.57) for the Talaromyces tree.

    Techniques:

    ML phylogenetic tree built using the ITS, BenA , CaM and rpb 2 concatenated dataset for species of the genus Talaromyces . Species of the section Trachyspermi are indicated in a blue background and those of the section Purpurei in yellow. Trichocoma paradoxa CBS 247.57 was chosen as out-group. Support in nodes is indicated above thick branches and is represented by posterior probabilities (BI analysis) of 0.95 and higher and/or bootstrap values (ML analysis) of 70% and higher. Fully supported branched (100% BS /1 PP) are indicated in bold. T = ex-type strain. Alignment length 2265 bp

    Journal: IMA Fungus

    Article Title: Diversity of xerotolerant and xerophilic fungi in honey

    doi: 10.1186/s43008-019-0021-7

    Figure Lengend Snippet: ML phylogenetic tree built using the ITS, BenA , CaM and rpb 2 concatenated dataset for species of the genus Talaromyces . Species of the section Trachyspermi are indicated in a blue background and those of the section Purpurei in yellow. Trichocoma paradoxa CBS 247.57 was chosen as out-group. Support in nodes is indicated above thick branches and is represented by posterior probabilities (BI analysis) of 0.95 and higher and/or bootstrap values (ML analysis) of 70% and higher. Fully supported branched (100% BS /1 PP) are indicated in bold. T = ex-type strain. Alignment length 2265 bp

    Article Snippet: Cunninghamella bertholletiae (CBS 693.68), Mucor plumbeus (DAOM 220743), Mucor racemosus (ATCC 42647), and Rhizopus oryzae (CBS 112.07 and CBS 130146) were used as outgroup for the LSU tree; Aphanoascus keratinophilus (IMI 319010) for the Myxotrichaceae taxa tree; and Trichocoma paradoxa (CBS 247.57) for the Talaromyces tree.

    Techniques: