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Roche pancreatic dnaase i
Pancreatic Dnaase I, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pancreatic dnaase i/product/Roche
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
pancreatic dnaase i - by Bioz Stars, 2020-04
86/100 stars

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Related Articles

TUNEL Assay:

Article Title: Nrf2 Inhibits Periodontal Ligament Stem Cell Apoptosis under Excessive Oxidative Stress
Article Snippet: Paragraph title: 4.8. TUNEL Staining ... Positive and negative controls were treated with 0.1 mg/mL pancreatic DNase I (F. Hoffmann-La Roche Ltd., Diagnostics Division, Basel, Swiss) or labeling, respectively.

Imaging:

Article Title: Nrf2 Inhibits Periodontal Ligament Stem Cell Apoptosis under Excessive Oxidative Stress
Article Snippet: Positive and negative controls were treated with 0.1 mg/mL pancreatic DNase I (F. Hoffmann-La Roche Ltd., Diagnostics Division, Basel, Swiss) or labeling, respectively. .. The percentage of stain-positive cells was detected with an image analyzing system (Leica Qwin.Plus, Leica Microsystem Imaging, Cambridge, UK), and the percentage of TUNEL-positive cells was calculated from the number of total 2000 cells.

Centrifugation:

Article Title: H5N1 Avian Influenza Virus Induces Apoptotic Cell Death in Mammalian Airway Epithelial Cells ▿H5N1 Avian Influenza Virus Induces Apoptotic Cell Death in Mammalian Airway Epithelial Cells ▿ †
Article Snippet: Airway epithelial cells collected by enzymatic treatment were pooled and isolated by centrifugation at 880 × g for 10 min at 4°C. .. The cells were resuspended in Ham's F-12 pen-strep containing crude pancreatic DNase I (0.5 mg/ml; Roche Molecular Biochemicals, Indianapolis, IN) and bovine serum albumin (10 mg/ml; Sigma-Aldrich, St. Louis, MO).

Filtration:

Article Title: H5N1 Avian Influenza Virus Induces Apoptotic Cell Death in Mammalian Airway Epithelial Cells ▿H5N1 Avian Influenza Virus Induces Apoptotic Cell Death in Mammalian Airway Epithelial Cells ▿ †
Article Snippet: The triturated tissues were filtered through stainless steel mesh with 1.5-mm pores, followed by filtration through nylon gauze with 0.5-mm pores and 70-μm cell strainers (BD Biosciences). .. The cells were resuspended in Ham's F-12 pen-strep containing crude pancreatic DNase I (0.5 mg/ml; Roche Molecular Biochemicals, Indianapolis, IN) and bovine serum albumin (10 mg/ml; Sigma-Aldrich, St. Louis, MO).

In Situ:

Article Title: Nrf2 Inhibits Periodontal Ligament Stem Cell Apoptosis under Excessive Oxidative Stress
Article Snippet: TUNEL Staining Apoptotic PDLSCs were assessed by TUNEL analysis with an in-situ Cell Death Fluorescein Detection Kit (Roche, Basel, Swiss), following the manufacturer’s instructions. .. Positive and negative controls were treated with 0.1 mg/mL pancreatic DNase I (F. Hoffmann-La Roche Ltd., Diagnostics Division, Basel, Swiss) or labeling, respectively.

Fluorescence:

Article Title: Nrf2 Inhibits Periodontal Ligament Stem Cell Apoptosis under Excessive Oxidative Stress
Article Snippet: Following this, they were mounted and analyzed under a confocal laser scanning fluorescence microscope (IX-71, Olympus, Tokyo, Japan). .. Positive and negative controls were treated with 0.1 mg/mL pancreatic DNase I (F. Hoffmann-La Roche Ltd., Diagnostics Division, Basel, Swiss) or labeling, respectively.

Mutagenesis:

Article Title: Extensive proteolysis of head and inner body proteins by a morphogenetic protease in the giant Pseudomonas aeruginosa phage ?KZ
Article Snippet: The culture was then transferred to 40°C and infected with the tailless mutant phage at a MOI of 5 and grown for 2 hr, 15 min until lysis was observed. .. Bacterial debris was removed by a low speed spin (10,400 x g , 5 min) and the sample treated with Pancreatic DNAase I (Roche)(1500 U, 37°C, 30 min).

Isolation:

Article Title: H5N1 Avian Influenza Virus Induces Apoptotic Cell Death in Mammalian Airway Epithelial Cells ▿H5N1 Avian Influenza Virus Induces Apoptotic Cell Death in Mammalian Airway Epithelial Cells ▿ †
Article Snippet: Paragraph title: pAEpC isolation and culture conditions. ... The cells were resuspended in Ham's F-12 pen-strep containing crude pancreatic DNase I (0.5 mg/ml; Roche Molecular Biochemicals, Indianapolis, IN) and bovine serum albumin (10 mg/ml; Sigma-Aldrich, St. Louis, MO).

Infection:

Article Title: Extensive proteolysis of head and inner body proteins by a morphogenetic protease in the giant Pseudomonas aeruginosa phage ?KZ
Article Snippet: The culture was then transferred to 40°C and infected with the tailless mutant phage at a MOI of 5 and grown for 2 hr, 15 min until lysis was observed. .. Bacterial debris was removed by a low speed spin (10,400 x g , 5 min) and the sample treated with Pancreatic DNAase I (Roche)(1500 U, 37°C, 30 min).

Purification:

Article Title: Extensive proteolysis of head and inner body proteins by a morphogenetic protease in the giant Pseudomonas aeruginosa phage ?KZ
Article Snippet: Paragraph title: Purification of phages ... Bacterial debris was removed by a low speed spin (10,400 x g , 5 min) and the sample treated with Pancreatic DNAase I (Roche)(1500 U, 37°C, 30 min).

Concentration Assay:

Article Title: Extensive proteolysis of head and inner body proteins by a morphogenetic protease in the giant Pseudomonas aeruginosa phage ?KZ
Article Snippet: Bacterial debris was removed by a low speed spin (10,400 x g , 5 min) and the sample treated with Pancreatic DNAase I (Roche)(1500 U, 37°C, 30 min). .. Heads were concentrated by slow stirring overnight at 4°C in 1M NaCl and 10% PEG (final concentration) and then pelleted (11,300 x g , 4°C, 30 min).

Incubation:

Article Title: H5N1 Avian Influenza Virus Induces Apoptotic Cell Death in Mammalian Airway Epithelial Cells ▿H5N1 Avian Influenza Virus Induces Apoptotic Cell Death in Mammalian Airway Epithelial Cells ▿ †
Article Snippet: Tissue cubes of approximately 3-cm edge length were injected with 0.15% (wt/vol) pronase (Calbiochem, San Diego, CA) in F-12 medium (Gibco Co., Carlsbad, CA) at a rate of 2 ml/tissue cube, followed by incubation in polyethylene tubes at 37°C for 1 h to accelerate enzymatic digestion. .. The cells were resuspended in Ham's F-12 pen-strep containing crude pancreatic DNase I (0.5 mg/ml; Roche Molecular Biochemicals, Indianapolis, IN) and bovine serum albumin (10 mg/ml; Sigma-Aldrich, St. Louis, MO).

Article Title: Nrf2 Inhibits Periodontal Ligament Stem Cell Apoptosis under Excessive Oxidative Stress
Article Snippet: Briefly, after experimental stimulation, the cells were washed with PBS and fixed in 4% paraformaldehyde for 15 min. After rinsing with PBS, the cells were incubated with a reaction mixture solution for 30 min at 37 °C in the dark. .. Positive and negative controls were treated with 0.1 mg/mL pancreatic DNase I (F. Hoffmann-La Roche Ltd., Diagnostics Division, Basel, Swiss) or labeling, respectively.

Labeling:

Article Title: Nrf2 Inhibits Periodontal Ligament Stem Cell Apoptosis under Excessive Oxidative Stress
Article Snippet: .. Positive and negative controls were treated with 0.1 mg/mL pancreatic DNase I (F. Hoffmann-La Roche Ltd., Diagnostics Division, Basel, Swiss) or labeling, respectively. .. The percentage of stain-positive cells was detected with an image analyzing system (Leica Qwin.Plus, Leica Microsystem Imaging, Cambridge, UK), and the percentage of TUNEL-positive cells was calculated from the number of total 2000 cells.

Protease Inhibitor:

Article Title: Extensive proteolysis of head and inner body proteins by a morphogenetic protease in the giant Pseudomonas aeruginosa phage ?KZ
Article Snippet: Bacterial debris was removed by a low speed spin (10,400 x g , 5 min) and the sample treated with Pancreatic DNAase I (Roche)(1500 U, 37°C, 30 min). .. Pellets were resuspended in 20 ml SM buffer [50 mM Tris-HCl (pH 7.5), 100 mM NaCl, 8 mM MgSO4 , 0.002% gelatin] containing Complete Protease Inhibitor (Roche) and then layered (5.8 ml/tube) onto CsCl step gradients composed of the following concentrations of CsCl: 1.59 g ml−1 (1.0 ml), 1.52 g ml−1 (0.75 ml), 1.5 g ml−1 (1.5 ml), 1.30 g ml−1 (1.5 ml) and 1.21 g ml−1 (1.5 ml).

Lysis:

Article Title: Extensive proteolysis of head and inner body proteins by a morphogenetic protease in the giant Pseudomonas aeruginosa phage ?KZ
Article Snippet: The culture was then transferred to 40°C and infected with the tailless mutant phage at a MOI of 5 and grown for 2 hr, 15 min until lysis was observed. .. Bacterial debris was removed by a low speed spin (10,400 x g , 5 min) and the sample treated with Pancreatic DNAase I (Roche)(1500 U, 37°C, 30 min).

Injection:

Article Title: H5N1 Avian Influenza Virus Induces Apoptotic Cell Death in Mammalian Airway Epithelial Cells ▿H5N1 Avian Influenza Virus Induces Apoptotic Cell Death in Mammalian Airway Epithelial Cells ▿ †
Article Snippet: Tissue cubes of approximately 3-cm edge length were injected with 0.15% (wt/vol) pronase (Calbiochem, San Diego, CA) in F-12 medium (Gibco Co., Carlsbad, CA) at a rate of 2 ml/tissue cube, followed by incubation in polyethylene tubes at 37°C for 1 h to accelerate enzymatic digestion. .. The cells were resuspended in Ham's F-12 pen-strep containing crude pancreatic DNase I (0.5 mg/ml; Roche Molecular Biochemicals, Indianapolis, IN) and bovine serum albumin (10 mg/ml; Sigma-Aldrich, St. Louis, MO).

Microscopy:

Article Title: Nrf2 Inhibits Periodontal Ligament Stem Cell Apoptosis under Excessive Oxidative Stress
Article Snippet: Following this, they were mounted and analyzed under a confocal laser scanning fluorescence microscope (IX-71, Olympus, Tokyo, Japan). .. Positive and negative controls were treated with 0.1 mg/mL pancreatic DNase I (F. Hoffmann-La Roche Ltd., Diagnostics Division, Basel, Swiss) or labeling, respectively.

Staining:

Article Title: Nrf2 Inhibits Periodontal Ligament Stem Cell Apoptosis under Excessive Oxidative Stress
Article Snippet: Paragraph title: 4.8. TUNEL Staining ... Positive and negative controls were treated with 0.1 mg/mL pancreatic DNase I (F. Hoffmann-La Roche Ltd., Diagnostics Division, Basel, Swiss) or labeling, respectively.

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    Roche dnase i
    Impact of <t>DNase</t> I treatment on B . pseudomallei biofilm formation. Static biofilms of B . pseudomallei strains L1, P1 and H777 in LB were treated with DNase I (0.01, 0.1 and 1 U/mL) at 0 h, 24 h or 45 h after inoculation and maintained for up to 48 h. Biofilm formation and eDNA concentration of the 2-day biofilms were assessed using crystal-violet absorbance (OD 620 ) and the QuantiFluor dsDNA System, respectively. DNase I buffer acted as control. Biofilm formation of each strain was examined in eight replicates and eDNA was quantified in duplicates, on three independent occasions. Data represents mean ± SD. * p
    Dnase I, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 2243 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnase i/product/Roche
    Average 99 stars, based on 2243 article reviews
    Price from $9.99 to $1999.99
    dnase i - by Bioz Stars, 2020-04
    99/100 stars
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    Impact of DNase I treatment on B . pseudomallei biofilm formation. Static biofilms of B . pseudomallei strains L1, P1 and H777 in LB were treated with DNase I (0.01, 0.1 and 1 U/mL) at 0 h, 24 h or 45 h after inoculation and maintained for up to 48 h. Biofilm formation and eDNA concentration of the 2-day biofilms were assessed using crystal-violet absorbance (OD 620 ) and the QuantiFluor dsDNA System, respectively. DNase I buffer acted as control. Biofilm formation of each strain was examined in eight replicates and eDNA was quantified in duplicates, on three independent occasions. Data represents mean ± SD. * p

    Journal: PLoS ONE

    Article Title: Extracellular DNA facilitates bacterial adhesion during Burkholderia pseudomallei biofilm formation

    doi: 10.1371/journal.pone.0213288

    Figure Lengend Snippet: Impact of DNase I treatment on B . pseudomallei biofilm formation. Static biofilms of B . pseudomallei strains L1, P1 and H777 in LB were treated with DNase I (0.01, 0.1 and 1 U/mL) at 0 h, 24 h or 45 h after inoculation and maintained for up to 48 h. Biofilm formation and eDNA concentration of the 2-day biofilms were assessed using crystal-violet absorbance (OD 620 ) and the QuantiFluor dsDNA System, respectively. DNase I buffer acted as control. Biofilm formation of each strain was examined in eight replicates and eDNA was quantified in duplicates, on three independent occasions. Data represents mean ± SD. * p

    Article Snippet: DNase I noticeably lowered eDNA concentrations in biofilm if the enzyme was added into the starting inoculum (0 h).

    Techniques: Concentration Assay

    Exogenous chromosomal DNA did not alter either untreated biofilm or DNase I-treated biofilm of B . pseudomallei H777. (A) Amount of 2-day B . pseudomallei H777 biofilm formed in LB, treated with DNase I, supplemented with either salmon sperm DNA (SS DNA) or B . pseudomallei genomic DNA (Bp DNA) compared to the controls. Data represents mean ± SD from three independent experiments. (B) Amount of 2-day B . pseudomallei H777 biofilm formed in LB after treatment with 0.01 U/mL DNase I for 3 h, followed by washing steps to remove DNase, and then supplemented with exogenous salmon sperm DNA or B . pseudomallei genomic DNA. Data represents mean ± SD from three independent experiments. ** p

    Journal: PLoS ONE

    Article Title: Extracellular DNA facilitates bacterial adhesion during Burkholderia pseudomallei biofilm formation

    doi: 10.1371/journal.pone.0213288

    Figure Lengend Snippet: Exogenous chromosomal DNA did not alter either untreated biofilm or DNase I-treated biofilm of B . pseudomallei H777. (A) Amount of 2-day B . pseudomallei H777 biofilm formed in LB, treated with DNase I, supplemented with either salmon sperm DNA (SS DNA) or B . pseudomallei genomic DNA (Bp DNA) compared to the controls. Data represents mean ± SD from three independent experiments. (B) Amount of 2-day B . pseudomallei H777 biofilm formed in LB after treatment with 0.01 U/mL DNase I for 3 h, followed by washing steps to remove DNase, and then supplemented with exogenous salmon sperm DNA or B . pseudomallei genomic DNA. Data represents mean ± SD from three independent experiments. ** p

    Article Snippet: DNase I noticeably lowered eDNA concentrations in biofilm if the enzyme was added into the starting inoculum (0 h).

    Techniques:

    DNase I treatment affects initial attachment and biofilm formation of B . pseudomallei . B . pseudomallei L1, P1 and H777 biofilms were grown in LB at 37°C. The biofilms were treated with DNase I (0.01 U/mL) at 0 h and 24 h post-seeding and maintained until 48 h. (A) CLSM images of DNase I treated biofilm structure and eDNA on coverslips. The 2-day biofilm architecture and quantity of eDNA were examined after staining with FITC-ConA (green) and TOTO-3 (red), respectively. The scale bars indicate 10 μm. The images were taken using a Zeiss 800 CLSM microscope (63× magnification). (B) COMSTAT image analysis of DNase I-treated B . pseudomallei biofilms and eDNA. Data represents mean ± SD of 18 images from three independent experiments. * p

    Journal: PLoS ONE

    Article Title: Extracellular DNA facilitates bacterial adhesion during Burkholderia pseudomallei biofilm formation

    doi: 10.1371/journal.pone.0213288

    Figure Lengend Snippet: DNase I treatment affects initial attachment and biofilm formation of B . pseudomallei . B . pseudomallei L1, P1 and H777 biofilms were grown in LB at 37°C. The biofilms were treated with DNase I (0.01 U/mL) at 0 h and 24 h post-seeding and maintained until 48 h. (A) CLSM images of DNase I treated biofilm structure and eDNA on coverslips. The 2-day biofilm architecture and quantity of eDNA were examined after staining with FITC-ConA (green) and TOTO-3 (red), respectively. The scale bars indicate 10 μm. The images were taken using a Zeiss 800 CLSM microscope (63× magnification). (B) COMSTAT image analysis of DNase I-treated B . pseudomallei biofilms and eDNA. Data represents mean ± SD of 18 images from three independent experiments. * p

    Article Snippet: DNase I noticeably lowered eDNA concentrations in biofilm if the enzyme was added into the starting inoculum (0 h).

    Techniques: Confocal Laser Scanning Microscopy, Staining, Microscopy

    TSA mimics estrogen induced activation of BRCA1 by increasing p300 dependent histone acetylation at the BRCA1 promoter. ( a ) Time course of TFF1 , NBR2 , and BRCA1 expression in MCF-7 cells treated 0–24 h with either E2, E2 + cycloheximide (10 µg ml −1 ), TSA (500 ng ml −1 ), or TSA + cycloheximide as indicated. Error bars represent the s.e.m. for N=2 independent biological replicates. ( b ) DNase I hypersensitivity profile of the BRCA1 promoter and an ( HBB ) locus control from MCF-7 cells treated with either estrogen or TSA. The error bars represent the s.e.m. for N=3 biological replicates. ( c ) Acetylated histone H3, acetylated histone H4, HDAC1, BRCA1, p130, and CtBP ChIP profiles at the BRCA1 promoter in control or MCF-7 cells treated 1 h with 500 ng ml −1 TSA. Error bars represent the s.e.m. for N=2 biological replicates. ( d ) Upper panel: TSA stimulated expression of BRCA1 nascent and mature RNA levels in either control or p300 depleted MCF-7. Error bars represent the s.e.m. for N=2 biological replicates. Lower panel: ChIP enrichment for H3 and H4 histone acetylation at the BRCA1 locus in control versus p300 depleted MCF-7 cells with or without TSA stimulation. Means from N=2 independent biological replicates are shown.

    Journal: Nature structural & molecular biology

    Article Title: Transcriptional regulation of BRCA1 expression by a metabolic switch

    doi: 10.1038/nsmb.1941

    Figure Lengend Snippet: TSA mimics estrogen induced activation of BRCA1 by increasing p300 dependent histone acetylation at the BRCA1 promoter. ( a ) Time course of TFF1 , NBR2 , and BRCA1 expression in MCF-7 cells treated 0–24 h with either E2, E2 + cycloheximide (10 µg ml −1 ), TSA (500 ng ml −1 ), or TSA + cycloheximide as indicated. Error bars represent the s.e.m. for N=2 independent biological replicates. ( b ) DNase I hypersensitivity profile of the BRCA1 promoter and an ( HBB ) locus control from MCF-7 cells treated with either estrogen or TSA. The error bars represent the s.e.m. for N=3 biological replicates. ( c ) Acetylated histone H3, acetylated histone H4, HDAC1, BRCA1, p130, and CtBP ChIP profiles at the BRCA1 promoter in control or MCF-7 cells treated 1 h with 500 ng ml −1 TSA. Error bars represent the s.e.m. for N=2 biological replicates. ( d ) Upper panel: TSA stimulated expression of BRCA1 nascent and mature RNA levels in either control or p300 depleted MCF-7. Error bars represent the s.e.m. for N=2 biological replicates. Lower panel: ChIP enrichment for H3 and H4 histone acetylation at the BRCA1 locus in control versus p300 depleted MCF-7 cells with or without TSA stimulation. Means from N=2 independent biological replicates are shown.

    Article Snippet: For each DNase I digestion, approximately 1×106 nuclei were harvested and resuspended in 200 ul of pre-warmed (37 ° C) Buffer A, supplemented with 6 mM CaCl2 , 75 mM NaCl, and the DNase I (0, 170, 340, and 680 units).

    Techniques: Activation Assay, Expressing, Chromatin Immunoprecipitation

    DNase I treatment of DNA and non-HAd Ft completely ablates the pro-inflammatory cytokine response. (A) Wild-type and AIM2 −/− BMDMs (2.5×10 5 cells/well) were incubated either in the absence or presence of DOTAP and 8 µg/ml of genomic DNA purified from Ft LVS. DNA was either untreated or incubated with DNase I as described in Methods . The levels of the cytokines released after 24 h were determined by CBA or ELISA. *** P

    Journal: PLoS ONE

    Article Title: Discordant Results Obtained with Francisella tularensis during In Vitro and In Vivo Immunological Studies Are Attributable to Compromised Bacterial Structural Integrity

    doi: 10.1371/journal.pone.0058513

    Figure Lengend Snippet: DNase I treatment of DNA and non-HAd Ft completely ablates the pro-inflammatory cytokine response. (A) Wild-type and AIM2 −/− BMDMs (2.5×10 5 cells/well) were incubated either in the absence or presence of DOTAP and 8 µg/ml of genomic DNA purified from Ft LVS. DNA was either untreated or incubated with DNase I as described in Methods . The levels of the cytokines released after 24 h were determined by CBA or ELISA. *** P

    Article Snippet: ***P < 0.001. (All results shown were subjected to One-way ANOVA with Bonferroni's Post-test). (E) & (F) Wild-type and AIM2−/− BMDMs were infected with Ft LVS cultivated in MHB either untreated or treated with DNase I at a MOI of 100.

    Techniques: Incubation, Purification, Crocin Bleaching Assay, Enzyme-linked Immunosorbent Assay

    Applicability of the ECM extraction method to  S . epidermidis ,  E . coli  and  P . aeruginosa  biofilms.A. Extracellular matrices extracted from  S . epidermidis  SE04 by the addition of 1.5 M NaCl were treated with or without dispersin B and were then applied to SDS-APGE. The gel was stained with CBB. An arrow indicates polysaccharides.B. Extracellular matrices of  E . coli  YMel and its isogenic  csgA  mutant YMel-1 were isolated with 1.5 M NaCl. Curli amyloid fibres in the ECM fraction were treated with or without formic acid. Purified curli was also used as a positive control. The proteins were analysed by Western blotting using anti-CsgA antibody. Arrows indicate monomeric and dimeric CsgA. FA, formic acid.C. Extracellular matrices of  P . aeruginosa  PAO1 were extracted with 1.5 M NaCl and were subjected to agarose gel electrophoresis. The extracted ECMs were treated with or without DNase I. The gel was stained with ethidium bromide. The positions of molecular mass markers in kilodaltons (kDa) (A and B) and kilobase pairs (kb) (C) are shown at the left of each panel respectively.

    Journal: Microbial Biotechnology

    Article Title: A refined technique for extraction of extracellular matrices from bacterial biofilms and its applicability

    doi: 10.1111/1751-7915.12155

    Figure Lengend Snippet: Applicability of the ECM extraction method to S . epidermidis , E . coli and P . aeruginosa biofilms.A. Extracellular matrices extracted from S . epidermidis  SE04 by the addition of 1.5 M NaCl were treated with or without dispersin B and were then applied to SDS-APGE. The gel was stained with CBB. An arrow indicates polysaccharides.B. Extracellular matrices of E . coli  YMel and its isogenic csgA mutant YMel-1 were isolated with 1.5 M NaCl. Curli amyloid fibres in the ECM fraction were treated with or without formic acid. Purified curli was also used as a positive control. The proteins were analysed by Western blotting using anti-CsgA antibody. Arrows indicate monomeric and dimeric CsgA. FA, formic acid.C. Extracellular matrices of P . aeruginosa  PAO1 were extracted with 1.5 M NaCl and were subjected to agarose gel electrophoresis. The extracted ECMs were treated with or without DNase I. The gel was stained with ethidium bromide. The positions of molecular mass markers in kilodaltons (kDa) (A and B) and kilobase pairs (kb) (C) are shown at the left of each panel respectively.

    Article Snippet: ECM-degrading enzymes Dispersin B from Aggregatibacter actinomycetemcomitans (20 μg ml−1 , Kane Biotech Inc., Manitoba, Canada), Proteinase K from Tritirachium album (100 μg ml−1 , Sigma, St Louis, MO, USA) and DNase I (100 U ml−1 , Roche Diagnostics, Mannheim, Germany) were used for dispersal of preformed biofilms or degradation of ECM components.

    Techniques: Staining, Mutagenesis, Isolation, Purification, Positive Control, Western Blot, Agarose Gel Electrophoresis