Journal: PLoS ONE

Article Title: Kinase Activity Profiling of Pneumococcal Pneumonia

doi: 10.1371/journal.pone.0018519

Figure Lengend Snippet: Western blot (a) and quantification of all CDK phosphorylated substrates (b) revealed a decrease in CDK activity. Quantification was performed for all positive signals on the blot and corrected for β-actin. Data are represented as mean ± SD of n = 3.

Article Snippet: Blots were done for: AMPK-α/pAMPK-α, GSK-3β/pGSK-3β, pan-CDK phospho-substrate, β-catenin (all antibodies from Cellsignaling Technology, Boston, MA, USA) and β-actin (Santacruz Biotechnology, Santa Cruz, CA, USA).

Techniques: Western Blot, Activity Assay

Journal: Cell Proliferation

Article Title: Effects of human epidermal growth factor gene‐transfected mesenchymal stem cells on fibroblast migration and proliferation

doi: 10.1111/cpr.12042

Figure Lengend Snippet: Effects of EGF ‐ MSC s on cell adhesion molecules and cell dynamics molecules in fibroblasts. Fibroblasts were treated with MSC CM, vector‐MSC CM or EGF‐MSC CM for 24 h. Expression of cell adhesion molecules (β‐catenin, N‐cadherin and pan‐cadherin) increased in EGF‐MSC CM‐treated fibroblasts. Expression of cell dynamics molecules (cofilin and p‐ezrin) increased in EGF‐MSC CM‐treated fibroblasts.

Article Snippet: Western blotting Protein in cell lysates was separated by 12% sodium dodecyl sulphate polyacrylamide gel electrophoresis, and protein was transferred to nitrocellulose membranes (Bio‐Rad, Hercules, CA, USA), probed with antibodies specific to β‐catenin, N‐cadherin, pan‐cadherin phospho‐(Ser) CDK substrate, phospho‐MAPK/CDK substrate, phospho‐(Ser)‐Arg‐X‐Tyr/Phe‐X‐pSer motif, phospho‐(Ser) 14‐3‐3 binding motif, cofilin, and p‐ezrin (Cell Signaling Technology, Danvers, MA, USA) diluted in TBST buffer (10 m m , pH 7.5 Tris‐HCl, 100 m m NaCl and 0.1% Tween‐20), followed by goat antirabbit IgG‐horseradish peroxidase (Santa Cruz Biotechnology, Santa Cruz, CA, USA).

Techniques: Plasmid Preparation, Expressing

Journal: Cell Proliferation

Article Title: Effects of human epidermal growth factor gene‐transfected mesenchymal stem cells on fibroblast migration and proliferation

doi: 10.1111/cpr.12042

Figure Lengend Snippet: Effects of EGF ‐ MSC s on phospho‐(Ser) kinase substrates of fibroblasts. Fibroblasts were treated with MSC CM, vector‐MSC CM or EGF‐MSC CM for 24 h. Exposure to EGF‐MSC CM‐treated fibroblasts resulted in increased expression of phospho‐(Ser) kinase substrates. (a) Expression of phospho‐(Ser) CDKs substrates, cell cycle‐related proteins, increased in EGF‐MSC CM‐treated fibroblasts. (b) Expression of phospho‐MAPK/CDK substrates, cell cycle and cell signalling‐related proteins increased in EGF‐MSC CM‐treated fibroblasts. (c) Expression of phospho‐(Ser) Arg‐X‐Tyr/Phe‐X‐pSer motif, cell survival and proliferation regulation‐related proteins increased in EGF‐MSC CM‐treated fibroblasts. (d) Expression of phospho‐(Ser) 14‐3‐3 binding motif, cell survival and proliferation regulation‐related proteins increased in EGF‐MSC CM‐treated fibroblasts.

Article Snippet: Western blotting Protein in cell lysates was separated by 12% sodium dodecyl sulphate polyacrylamide gel electrophoresis, and protein was transferred to nitrocellulose membranes (Bio‐Rad, Hercules, CA, USA), probed with antibodies specific to β‐catenin, N‐cadherin, pan‐cadherin phospho‐(Ser) CDK substrate, phospho‐MAPK/CDK substrate, phospho‐(Ser)‐Arg‐X‐Tyr/Phe‐X‐pSer motif, phospho‐(Ser) 14‐3‐3 binding motif, cofilin, and p‐ezrin (Cell Signaling Technology, Danvers, MA, USA) diluted in TBST buffer (10 m m , pH 7.5 Tris‐HCl, 100 m m NaCl and 0.1% Tween‐20), followed by goat antirabbit IgG‐horseradish peroxidase (Santa Cruz Biotechnology, Santa Cruz, CA, USA).

Techniques: Plasmid Preparation, Expressing, Binding Assay