Journal: bioRxiv

Article Title: Exposure to perfluorooctanoic acid accelerates Drosophila melanogaster juvenile development and disrupts mitochondrial metabolism

doi: 10.64898/2026.06.14.730922

Figure Lengend Snippet: Larvae were exposed to 0, 0.036, or 3.6 µM PFOA and the resulting adult female and male flies were analyzed 5 days after eclosion using targeted LC-MS analysis to measure the relative abundance of (A-B) palmitoyl-L-carnitine (CAR 16:0) and (C-D) oleoyl-L-carnitine (CAR 18:1). For all analyses, n = 6 biological replicates per condition, 20 flies per replicate. Statistical significance for targeted analyses was determined using a one-way ANOVA followed by Dunnett’s multiple comparisons test for the female CAR 18:1 analysis and Kruskal-Wallis test followed by a Dunn’s multiple comparisons test for the male CAR 18:1, and male and female CAR 16:0. Significance values relative to the unexposed controls. * p < 0.05.

Article Snippet: Targeted measurements were performed for two acyl-carnitines for which authentic standards were available: palmitoyl-L-carnitine (C16:0; Avanti Polar Lipids, 870323P) and oleoyl-L-carnitine (C18:1; Avanti Polar Lipids, 870321P).

Techniques: Liquid Chromatography with Mass Spectroscopy