anti cd105 pa5 12511  (Thermo Fisher)


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    Structured Review

    Thermo Fisher anti cd105 pa5 12511
    Hepatocyte-specific deletion of HIF-2α does not affect HIF-1α expression. Liver expression of HIF-1α evaluated by ( A ) Q-PCR and ( B ) immunohistochemical analysis in HCCs from 9 WT or from 6 hHIF-2α –/– . Original magnification is indicated. ( C ) Gene expression of vascular endothelial growth factor (VEGF), FLK1, and vascular endothelialcadherin evaluated by Q-PCR in HCC tumor masses from 9 WT mice or from 6 hHIF-2α –/– mice ( C ). The mRNA values are expressed as the fold increase over control values after normalization to the TATA box binding protein gene expression. Results are expressed as means ± SD. Boxes include the values within the 25th and 75th percentiles, whereas the horizontal bars represent the medians. The extremities of the vertical bars (10th–90th percentile) comprise 80% of the values. Statistical differences were assessed by the Student t test or the Mann–Whitney test for nonparametric values. ( D ) WB analysis of <t>cd105</t> protein levels in HCCs from 7 WT mice or from 5 hHIF-2α –/– mice. For the Western blot analysis, Bio-Rad Quantity One software was used to perform the densitometric analysis. Equal loading was evaluated by reprobing membranes for β-actin. Statistical differences were assessed by the Student t test or the Mann–Whitney test for nonparametric values.
    Anti Cd105 Pa5 12511, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cd105 pa5 12511/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti cd105 pa5 12511 - by Bioz Stars, 2023-06
    86/100 stars

    Images

    1) Product Images from "Hepatocyte-Specific Deletion of HIF2α Prevents NASH-Related Liver Carcinogenesis by Decreasing Cancer Cell Proliferation"

    Article Title: Hepatocyte-Specific Deletion of HIF2α Prevents NASH-Related Liver Carcinogenesis by Decreasing Cancer Cell Proliferation

    Journal: Cellular and Molecular Gastroenterology and Hepatology

    doi: 10.1016/j.jcmgh.2021.10.002

    Hepatocyte-specific deletion of HIF-2α does not affect HIF-1α expression. Liver expression of HIF-1α evaluated by ( A ) Q-PCR and ( B ) immunohistochemical analysis in HCCs from 9 WT or from 6 hHIF-2α –/– . Original magnification is indicated. ( C ) Gene expression of vascular endothelial growth factor (VEGF), FLK1, and vascular endothelialcadherin evaluated by Q-PCR in HCC tumor masses from 9 WT mice or from 6 hHIF-2α –/– mice ( C ). The mRNA values are expressed as the fold increase over control values after normalization to the TATA box binding protein gene expression. Results are expressed as means ± SD. Boxes include the values within the 25th and 75th percentiles, whereas the horizontal bars represent the medians. The extremities of the vertical bars (10th–90th percentile) comprise 80% of the values. Statistical differences were assessed by the Student t test or the Mann–Whitney test for nonparametric values. ( D ) WB analysis of cd105 protein levels in HCCs from 7 WT mice or from 5 hHIF-2α –/– mice. For the Western blot analysis, Bio-Rad Quantity One software was used to perform the densitometric analysis. Equal loading was evaluated by reprobing membranes for β-actin. Statistical differences were assessed by the Student t test or the Mann–Whitney test for nonparametric values.
    Figure Legend Snippet: Hepatocyte-specific deletion of HIF-2α does not affect HIF-1α expression. Liver expression of HIF-1α evaluated by ( A ) Q-PCR and ( B ) immunohistochemical analysis in HCCs from 9 WT or from 6 hHIF-2α –/– . Original magnification is indicated. ( C ) Gene expression of vascular endothelial growth factor (VEGF), FLK1, and vascular endothelialcadherin evaluated by Q-PCR in HCC tumor masses from 9 WT mice or from 6 hHIF-2α –/– mice ( C ). The mRNA values are expressed as the fold increase over control values after normalization to the TATA box binding protein gene expression. Results are expressed as means ± SD. Boxes include the values within the 25th and 75th percentiles, whereas the horizontal bars represent the medians. The extremities of the vertical bars (10th–90th percentile) comprise 80% of the values. Statistical differences were assessed by the Student t test or the Mann–Whitney test for nonparametric values. ( D ) WB analysis of cd105 protein levels in HCCs from 7 WT mice or from 5 hHIF-2α –/– mice. For the Western blot analysis, Bio-Rad Quantity One software was used to perform the densitometric analysis. Equal loading was evaluated by reprobing membranes for β-actin. Statistical differences were assessed by the Student t test or the Mann–Whitney test for nonparametric values.

    Techniques Used: Expressing, Immunohistochemical staining, Binding Assay, MANN-WHITNEY, Western Blot, Software

    pa5 12511  (Thermo Fisher)


    Bioz Verified Symbol Thermo Fisher is a verified supplier
    Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
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  • 86

    Structured Review

    Thermo Fisher pa5 12511
    Antibodies Used in This Study.
    Pa5 12511, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pa5 12511/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pa5 12511 - by Bioz Stars, 2023-06
    86/100 stars

    Images

    1) Product Images from "Quantitative Analysis of SSEA3+ Cells from Human Umbilical Cord after Magnetic Sorting"

    Article Title: Quantitative Analysis of SSEA3+ Cells from Human Umbilical Cord after Magnetic Sorting

    Journal: Cell Transplantation

    doi: 10.1177/0963689719844260

    Antibodies Used in This Study.
    Figure Legend Snippet: Antibodies Used in This Study.

    Techniques Used: Immunocytochemistry, Flow Cytometry, Staining

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  • 86
    Thermo Fisher anti cd105 pa5 12511
    Hepatocyte-specific deletion of HIF-2α does not affect HIF-1α expression. Liver expression of HIF-1α evaluated by ( A ) Q-PCR and ( B ) immunohistochemical analysis in HCCs from 9 WT or from 6 hHIF-2α –/– . Original magnification is indicated. ( C ) Gene expression of vascular endothelial growth factor (VEGF), FLK1, and vascular endothelialcadherin evaluated by Q-PCR in HCC tumor masses from 9 WT mice or from 6 hHIF-2α –/– mice ( C ). The mRNA values are expressed as the fold increase over control values after normalization to the TATA box binding protein gene expression. Results are expressed as means ± SD. Boxes include the values within the 25th and 75th percentiles, whereas the horizontal bars represent the medians. The extremities of the vertical bars (10th–90th percentile) comprise 80% of the values. Statistical differences were assessed by the Student t test or the Mann–Whitney test for nonparametric values. ( D ) WB analysis of <t>cd105</t> protein levels in HCCs from 7 WT mice or from 5 hHIF-2α –/– mice. For the Western blot analysis, Bio-Rad Quantity One software was used to perform the densitometric analysis. Equal loading was evaluated by reprobing membranes for β-actin. Statistical differences were assessed by the Student t test or the Mann–Whitney test for nonparametric values.
    Anti Cd105 Pa5 12511, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cd105 pa5 12511/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti cd105 pa5 12511 - by Bioz Stars, 2023-06
    86/100 stars
      Buy from Supplier

    86
    Thermo Fisher pa5 12511
    Antibodies Used in This Study.
    Pa5 12511, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pa5 12511/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pa5 12511 - by Bioz Stars, 2023-06
    86/100 stars
      Buy from Supplier

    Image Search Results


    Hepatocyte-specific deletion of HIF-2α does not affect HIF-1α expression. Liver expression of HIF-1α evaluated by ( A ) Q-PCR and ( B ) immunohistochemical analysis in HCCs from 9 WT or from 6 hHIF-2α –/– . Original magnification is indicated. ( C ) Gene expression of vascular endothelial growth factor (VEGF), FLK1, and vascular endothelialcadherin evaluated by Q-PCR in HCC tumor masses from 9 WT mice or from 6 hHIF-2α –/– mice ( C ). The mRNA values are expressed as the fold increase over control values after normalization to the TATA box binding protein gene expression. Results are expressed as means ± SD. Boxes include the values within the 25th and 75th percentiles, whereas the horizontal bars represent the medians. The extremities of the vertical bars (10th–90th percentile) comprise 80% of the values. Statistical differences were assessed by the Student t test or the Mann–Whitney test for nonparametric values. ( D ) WB analysis of cd105 protein levels in HCCs from 7 WT mice or from 5 hHIF-2α –/– mice. For the Western blot analysis, Bio-Rad Quantity One software was used to perform the densitometric analysis. Equal loading was evaluated by reprobing membranes for β-actin. Statistical differences were assessed by the Student t test or the Mann–Whitney test for nonparametric values.

    Journal: Cellular and Molecular Gastroenterology and Hepatology

    Article Title: Hepatocyte-Specific Deletion of HIF2α Prevents NASH-Related Liver Carcinogenesis by Decreasing Cancer Cell Proliferation

    doi: 10.1016/j.jcmgh.2021.10.002

    Figure Lengend Snippet: Hepatocyte-specific deletion of HIF-2α does not affect HIF-1α expression. Liver expression of HIF-1α evaluated by ( A ) Q-PCR and ( B ) immunohistochemical analysis in HCCs from 9 WT or from 6 hHIF-2α –/– . Original magnification is indicated. ( C ) Gene expression of vascular endothelial growth factor (VEGF), FLK1, and vascular endothelialcadherin evaluated by Q-PCR in HCC tumor masses from 9 WT mice or from 6 hHIF-2α –/– mice ( C ). The mRNA values are expressed as the fold increase over control values after normalization to the TATA box binding protein gene expression. Results are expressed as means ± SD. Boxes include the values within the 25th and 75th percentiles, whereas the horizontal bars represent the medians. The extremities of the vertical bars (10th–90th percentile) comprise 80% of the values. Statistical differences were assessed by the Student t test or the Mann–Whitney test for nonparametric values. ( D ) WB analysis of cd105 protein levels in HCCs from 7 WT mice or from 5 hHIF-2α –/– mice. For the Western blot analysis, Bio-Rad Quantity One software was used to perform the densitometric analysis. Equal loading was evaluated by reprobing membranes for β-actin. Statistical differences were assessed by the Student t test or the Mann–Whitney test for nonparametric values.

    Article Snippet: The following antibodies were used: anti–HIF-1α (NB100-479) and anti–HIF-2α (NB100-122) from Novus Biologicals (Cambridge, UK); anti-CD105 (PA5-12511), anti-PCNA (PA5-27214), and anti-SB3 (PA5-30164) from ThermoFisher Scientific (Rockford, IL); anti–α-SMA (M0851) was from DAKO (Agilent, St Clara, CA); anti-SB3 (GTX32866) was from GeneTex (Irvine, CA); anti-F4/80 (14-4801-82) was from eBioscience (Affymetrix, St Clara, CA); anti-YAP (sc-15407), anti-c-MYC (sc-788), anti-SB3 (sc21767), anti-p53 (sc-6243), anti-p21 (sc-817), anti-ERK (sc-94), anti-JNK (sc-571), antivinculin (sc-73614), anti–p-Akt1/2/3 (sc-7985-R), and anti-Akt1/2/3 (sc-8312) were from Santa Cruz Biotechnology (Dallas, TX); anti-phospho-ERK (extracellular-regulated kinase, [4696]) and anti–anti-phospho-JNK (c-Jun aminoterminal kinase [9255]) were from Cell Signaling Technology (Danvers, MA); and anti–β-actin (A5441) was from Sigma Aldrich (St. Louis, MO).

    Techniques: Expressing, Immunohistochemical staining, Binding Assay, MANN-WHITNEY, Western Blot, Software

    Antibodies Used in This Study.

    Journal: Cell Transplantation

    Article Title: Quantitative Analysis of SSEA3+ Cells from Human Umbilical Cord after Magnetic Sorting

    doi: 10.1177/0963689719844260

    Figure Lengend Snippet: Antibodies Used in This Study.

    Article Snippet: CD105 , Rabbit anti-human , 1:500 , Thermo Fisher Scientific , PA5-12511 , Immunocytochemistry.

    Techniques: Immunocytochemistry, Flow Cytometry, Staining