p38 mapk (Cell Signaling Technology Inc)
99
|
Name:
Phospho p38 MAPK Thr180 Tyr182 Blocking Peptide
Description:
This peptide is used to specifically block Phospho p38 MAPK Thr180 Tyr182 12F8 Rabbit mAb 4631 reactivity
Catalog Number:
1170
Price:
None
Category:
Experimental Controls
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Buy from Supplier |
Structured Review
Cell Signaling Technology Inc
p38 mapk
This peptide is used to specifically block Phospho p38 MAPK Thr180 Tyr182 12F8 Rabbit mAb 4631 reactivity
https://www.bioz.com/result/p38 mapk/product/Cell Signaling Technology Inc
Average 99 stars, based on 1 article reviews
Price from $9.99 to $1999.99
This peptide is used to specifically block Phospho p38 MAPK Thr180 Tyr182 12F8 Rabbit mAb 4631 reactivity
https://www.bioz.com/result/p38 mapk/product/Cell Signaling Technology Inc
Average 99 stars, based on 1 article reviews
Price from $9.99 to $1999.99
p38 mapk - by Bioz Stars,
2021-03
99/100 stars
Images
1) Product Images from "Aspirin alleviates cardiac fibrosis in mice by inhibiting autophagy"
Article Title: Aspirin alleviates cardiac fibrosis in mice by inhibiting autophagy
Journal: Acta Pharmacologica Sinica
doi: 10.1038/aps.2016.143
Figure Legend Snippet: p38 MAPK induced an autophagy-dependent pathway in ASA-regulated CF proliferation. (A) CFs exposed to hypoxia were treated with doses of ASA at 0.5, 2.5, and 5 mmol/L for 12 h, and then the changes of phosphorylated p38, ERK, JNK were detected by Western blot. (B) Cardiac fibroblasts were pre-treated with the p38 inhibitor SB203580 (10 μmol/L) for 1 h and were then treated with 2.5 mmol/L ASA for 12 h. Western blot results showed the changes of LC3-II, phosphorylated Akt and cleaved PARP 1. Mean±SEM. n =3, ** P
Techniques Used: Western Blot
2) Product Images from "Reactive microglia drive tau pathology and contribute to the spreading of pathological tau in the brain"
Article Title: Reactive microglia drive tau pathology and contribute to the spreading of pathological tau in the brain
Journal: Brain
doi: 10.1093/brain/awv081
Figure Legend Snippet: CX3CR1 deficiency accelerates tau hyperphosphorylation and p38 MAPK activation in hTau mice at early stages of development. ( A ) Western blot analysis of hippocampal lysates shows elevated tau phosphorylation on AT8, AT180 and PHF-1 sites and activation of p38 MAPK (pT180/pY182) in hTau Cx3cr1 −/− mice at 2 months of age. No significant alterations in total tau (Tau5 antibody) or total (t) p38 MAPK levels. GAPDH was the loading control. Lysates from tau knockout mice were included in the Mapt −/− lane and show no expression of tau. ( B–E ) Quantification of western blots for AT8, AT180, PHF-1 and phospho (p)-p38 MAPK reveal a statistically significant [ n = 5 per group for 2, 12 and 24-month-old groups; n = 6 for 6-month-old group; mean ± SEM integrated density value ratio for respective epitopes (* P
Techniques Used: Activation Assay, Mouse Assay, Western Blot, Knock-Out, Expressing
Figure Legend Snippet: Inclusion of IL-1Ra in the microglial inoculum reduces overall reactivity for phosphorylated p38 MAPK within the recipient mouse brain. ( A , B and E ) Immunostained brain sections displaying overall reduced phosphorylated (p)-p38 MAPK (pT180/pY182) immunoreactivity around the needle track when the IL-1Ra was included with microglia from 6-month-old hTau Cx3cr1 −/− donor mice ( B ) compared to those receiving hTau Cx3cr1 −/− donor microglia alone ( A ). Scale bar = 30 µm. Quantification of percentage p-p38 MAPK immunoreactive area revealed a statistically significant (* P
Techniques Used: Mouse Assay
Related Articles
Binding Assay:Article Title: Treatment with TO901317, a synthetic liver X receptor agonist, reduces brain damage and attenuates neuroinflammation in experimental intracerebral hemorrhage Article Snippet: .. Equal amounts of protein (35 to 50 μg protein in 20 μL for tissue samples and 20 μg protein in 20 μL for cell lysates) were separated by sodium dodecyl sulfate-polyacrylamide gels, transferred to Immobilon-P membranes (Millipore), blocked using 5 % milk in PBS containing 0.1 % Tween-20, and probed overnight at 4 °C with primary antibodies including mouse anti-LXR-α (1:1000; Abcam), rabbit anti-LXR-β (1:1000; Santa Cruz), mouse anti-ATP binding cassette transporter-1 (ABCA-1; 1:1000; Abcam), mouse anti-iNOS (1:200; Sigma), rabbit anti-COX-2 (1:1000; Cayman), rabbit anti-NF-κB p65 (1:1000; Santa Cruz), rabbit phospho-p38 (1:1000; Cell Signaling, Danvers, MA, USA), Article Title: Nitroxides Mitigate Neutrophil-Mediated Damage to the Myocardium after Experimental Myocardial Infarction in Rats Article Snippet: PVDF membranes were imaged on the Bio-Rad ChemiDoc imaging system. .. Non-specific binding was minimised by incubating the membranes with 5% (w /v ) BSA in Tris Buffered Saline with Tween-20 (TBST) [composition: 25 mM Tris base, 140 mM sodium chloride (NaCl), 4 mM potassium chloride (KCl), 0.1% Tween 20 in 1 L of MilliQ water] blocking solution on a rocker at 22 °C for 4 h. p-p38 was detected by incubation of the membrane with 1:1000 (v /v ) Fluorescence In Situ Hybridization:Article Title: PGC-1α and exercise intensity dependent adaptations in mouse skeletal muscle Article Snippet: Phosphorylation levels and protein content were measured by SDS-PAGE and western blotting using self-casted gels. .. PVDF membranes were blocked in 3% fish gel, and protein and phosphorylation sites were measured using primary antibodies against AMPK Thr172 (#2535S, Cell Signaling), AMPKα2 protein (#G3013, Santa Cruz Biotechnology), Expressing:Article Title: 4-1BB Signaling Promotes Alveolar Macrophages-Mediated Pro-Fibrotic Responses and Crystalline Silica-Induced Pulmonary Fibrosis in Mice Article Snippet: Western Blot AnalysisWestern blotting was performed essentially as previously described ( ). .. The expression of CD137 (1:500, rat, Abcam, Cambridge, UK), ASK1 (1:2,000, rabbit, Abcam), phospho-ASK1 [1:500, rabbit, Cell Signaling Technology (CST), Danvers, MA, USA], Western Blot:Article Title: 4-1BB Signaling Promotes Alveolar Macrophages-Mediated Pro-Fibrotic Responses and Crystalline Silica-Induced Pulmonary Fibrosis in Mice Article Snippet: Western Blot AnalysisWestern blotting was performed essentially as previously described ( ). .. The expression of CD137 (1:500, rat, Abcam, Cambridge, UK), ASK1 (1:2,000, rabbit, Abcam), phospho-ASK1 [1:500, rabbit, Cell Signaling Technology (CST), Danvers, MA, USA], Article Title: Active components from Radix Scrophulariae inhibits the ventricular remodeling induced by hypertension in rats Article Snippet: Western blotting Western blots were measured as previously described (Huang and Chen ). .. Briefly, active and total form of ERK1/2, JNK and p38 MAPK accumulation were detected by Western blot analysis with using the following antigenes: phospho-P44/42MAPK (ERK 1/2) Thr202 –Tyr204 (1:2000), phospho-SAPK/JNK Thr183 –Tyr185 (1:1000), Blocking Assay:Article Title: Nitroxides Mitigate Neutrophil-Mediated Damage to the Myocardium after Experimental Myocardial Infarction in Rats Article Snippet: PVDF membranes were imaged on the Bio-Rad ChemiDoc imaging system. .. Non-specific binding was minimised by incubating the membranes with 5% (w /v ) BSA in Tris Buffered Saline with Tween-20 (TBST) [composition: 25 mM Tris base, 140 mM sodium chloride (NaCl), 4 mM potassium chloride (KCl), 0.1% Tween 20 in 1 L of MilliQ water] blocking solution on a rocker at 22 °C for 4 h. p-p38 was detected by incubation of the membrane with 1:1000 (v /v ) Incubation:Article Title: Nitroxides Mitigate Neutrophil-Mediated Damage to the Myocardium after Experimental Myocardial Infarction in Rats Article Snippet: PVDF membranes were imaged on the Bio-Rad ChemiDoc imaging system. .. Non-specific binding was minimised by incubating the membranes with 5% (w /v ) BSA in Tris Buffered Saline with Tween-20 (TBST) [composition: 25 mM Tris base, 140 mM sodium chloride (NaCl), 4 mM potassium chloride (KCl), 0.1% Tween 20 in 1 L of MilliQ water] blocking solution on a rocker at 22 °C for 4 h. p-p38 was detected by incubation of the membrane with 1:1000 (v /v ) |
