p38 mapk inhibitor  (InvivoGen)

 
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    Name:
    SB202190
    Description:
    MAP Kinase Inhibitor
    Catalog Number:
    TLRL-SB90
    Price:
    None
    Category:
    SB202190 NF κB MAPK Activation Inhibitors Immunomodulators Inhibitors
    Size:
    5 mg
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    Structured Review

    InvivoGen p38 mapk inhibitor
    IL-32γ induces phosphorylation of <t>p38</t> <t>MAPK</t> in human blood neutrophils. Freshly isolated neutrophils were incubated in the presence of IL-32γ (500 ng.ml −1 ) or its vehicle (HBSS) for the indicated time. Phosphorylation of p38 and total p38 were detected by immunoblot. Representative of 4 different donors.
    MAP Kinase Inhibitor
    https://www.bioz.com/result/p38 mapk inhibitor/product/InvivoGen
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    p38 mapk inhibitor - by Bioz Stars, 2021-04
    93/100 stars

    Images

    1) Product Images from "IL-32γ Delays Spontaneous Apoptosis of Human Neutrophils through MCL-1, Regulated Primarily by the p38 MAPK Pathway"

    Article Title: IL-32γ Delays Spontaneous Apoptosis of Human Neutrophils through MCL-1, Regulated Primarily by the p38 MAPK Pathway

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0109256

    IL-32γ induces phosphorylation of p38 MAPK in human blood neutrophils. Freshly isolated neutrophils were incubated in the presence of IL-32γ (500 ng.ml −1 ) or its vehicle (HBSS) for the indicated time. Phosphorylation of p38 and total p38 were detected by immunoblot. Representative of 4 different donors.
    Figure Legend Snippet: IL-32γ induces phosphorylation of p38 MAPK in human blood neutrophils. Freshly isolated neutrophils were incubated in the presence of IL-32γ (500 ng.ml −1 ) or its vehicle (HBSS) for the indicated time. Phosphorylation of p38 and total p38 were detected by immunoblot. Representative of 4 different donors.

    Techniques Used: Isolation, Incubation

    Related Articles

    Synthesized:

    Article Title: The Complement Anaphylatoxins, C5a and C3a, Suppress Interferon-Beta Production in Response to Listeria monocytogenes by Inhibition of the Cyclic Dinucleotide-Activated Cytosolic Surveillance Pathway
    Article Snippet: .. The C5aR agonist (C5aA) (RAARISLGPRSIKAFTE) ( ) and C3aR agonist (C3aA) (WWTRRWRGDKLGLAR) ( ) were synthesized by GenScript with greater than 95% purity. c-di-AMP, BX795, Wortmannin, U0126, and SB202190 were purchased from Invivogen. .. LPS from E. coli was purchased from List Labs, and CGI-1746 was purchased from ApexBio.

    Negative Control:

    Article Title: IL-32γ Delays Spontaneous Apoptosis of Human Neutrophils through MCL-1, Regulated Primarily by the p38 MAPK Pathway
    Article Snippet: The inhibitors of PI3K/Akt (LY294002) and MEK-1 (U0126) were obtained from Cayman Chemical (Ann Arbor, Michigan, USA). .. The JNK inhibitor (SP600125) was from Calbiochem (San Diego, CA, USA), p38 MAPK inhibitor (SB202190) was obtained from Invivogen (San Diego, CA, USA), and MAPK inhibitor negative control (SB202474) was from Life Technologies (Carlsbad, CA, USA). .. The fluorescein active caspase-3 staining kit was purchased from BioVision (Milpitas, CA, USA) and the caspase-3 (active form) apoptosis kit was from BD Biosciences (San Jose, CA, USA).

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  • 93
    InvivoGen p38 mapk inhibitor
    IL-32γ induces phosphorylation of <t>p38</t> <t>MAPK</t> in human blood neutrophils. Freshly isolated neutrophils were incubated in the presence of IL-32γ (500 ng.ml −1 ) or its vehicle (HBSS) for the indicated time. Phosphorylation of p38 and total p38 were detected by immunoblot. Representative of 4 different donors.
    P38 Mapk Inhibitor, supplied by InvivoGen, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p38 mapk inhibitor/product/InvivoGen
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    p38 mapk inhibitor - by Bioz Stars, 2021-04
    93/100 stars
      Buy from Supplier

    95
    InvivoGen p38 mapk
    Effect of IL-1β pathway inhibitors on IL-1β mediated up-regulation of Cx43 mRNA expression in CFs. (A–E) Quiesced cells were stimulated with 10 ng/mL IL-1β for 24 h and inhibitors added at (A) the same time or (B and C) 30 min (D and E) to 1 h prior to stimulation. Cells maintained in 0.5% FBS were used as a control and 0.1% DMSO used as a vehicle control where required. (A, B and D) Cx43 mRNA data is represented as relative quantification (RQ) ±rq max normalised to UBC. (C and E) Representative western blot showing Cx43 and β-tubulin control expression in replicate wells. The concentration of inhibitors used were (A) 0.25, 0.5 or 1 μg/mL human recombinant IL-1β receptor antagonist (IL-1Ra) ( n= 3), (B and C) 100 μM SC-514 (SC) ( n= 3), (D and E) 10 μM SB203580 (SB), 20 μM SP600125 (SP) or 10 μM AZD6244 (AZ) for inhibition of <t>p38</t> <t>MAPK,</t> JNK or MEK1/2 respectively ( n= 5 for mRNA and n= 3 for protein). ** P
    P38 Mapk, supplied by InvivoGen, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p38 mapk/product/InvivoGen
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    p38 mapk - by Bioz Stars, 2021-04
    95/100 stars
      Buy from Supplier

    Image Search Results


    IL-32γ induces phosphorylation of p38 MAPK in human blood neutrophils. Freshly isolated neutrophils were incubated in the presence of IL-32γ (500 ng.ml −1 ) or its vehicle (HBSS) for the indicated time. Phosphorylation of p38 and total p38 were detected by immunoblot. Representative of 4 different donors.

    Journal: PLoS ONE

    Article Title: IL-32γ Delays Spontaneous Apoptosis of Human Neutrophils through MCL-1, Regulated Primarily by the p38 MAPK Pathway

    doi: 10.1371/journal.pone.0109256

    Figure Lengend Snippet: IL-32γ induces phosphorylation of p38 MAPK in human blood neutrophils. Freshly isolated neutrophils were incubated in the presence of IL-32γ (500 ng.ml −1 ) or its vehicle (HBSS) for the indicated time. Phosphorylation of p38 and total p38 were detected by immunoblot. Representative of 4 different donors.

    Article Snippet: The JNK inhibitor (SP600125) was from Calbiochem (San Diego, CA, USA), p38 MAPK inhibitor (SB202190) was obtained from Invivogen (San Diego, CA, USA), and MAPK inhibitor negative control (SB202474) was from Life Technologies (Carlsbad, CA, USA).

    Techniques: Isolation, Incubation

    Effect of IL-1β pathway inhibitors on IL-1β mediated up-regulation of Cx43 mRNA expression in CFs. (A–E) Quiesced cells were stimulated with 10 ng/mL IL-1β for 24 h and inhibitors added at (A) the same time or (B and C) 30 min (D and E) to 1 h prior to stimulation. Cells maintained in 0.5% FBS were used as a control and 0.1% DMSO used as a vehicle control where required. (A, B and D) Cx43 mRNA data is represented as relative quantification (RQ) ±rq max normalised to UBC. (C and E) Representative western blot showing Cx43 and β-tubulin control expression in replicate wells. The concentration of inhibitors used were (A) 0.25, 0.5 or 1 μg/mL human recombinant IL-1β receptor antagonist (IL-1Ra) ( n= 3), (B and C) 100 μM SC-514 (SC) ( n= 3), (D and E) 10 μM SB203580 (SB), 20 μM SP600125 (SP) or 10 μM AZD6244 (AZ) for inhibition of p38 MAPK, JNK or MEK1/2 respectively ( n= 5 for mRNA and n= 3 for protein). ** P

    Journal: Heliyon

    Article Title: Regulation of connexin 43 by interleukin 1β in adult rat cardiac fibroblasts and effects in an adult rat cardiac myocyte: fibroblast co-culture model

    doi: 10.1016/j.heliyon.2019.e03031

    Figure Lengend Snippet: Effect of IL-1β pathway inhibitors on IL-1β mediated up-regulation of Cx43 mRNA expression in CFs. (A–E) Quiesced cells were stimulated with 10 ng/mL IL-1β for 24 h and inhibitors added at (A) the same time or (B and C) 30 min (D and E) to 1 h prior to stimulation. Cells maintained in 0.5% FBS were used as a control and 0.1% DMSO used as a vehicle control where required. (A, B and D) Cx43 mRNA data is represented as relative quantification (RQ) ±rq max normalised to UBC. (C and E) Representative western blot showing Cx43 and β-tubulin control expression in replicate wells. The concentration of inhibitors used were (A) 0.25, 0.5 or 1 μg/mL human recombinant IL-1β receptor antagonist (IL-1Ra) ( n= 3), (B and C) 100 μM SC-514 (SC) ( n= 3), (D and E) 10 μM SB203580 (SB), 20 μM SP600125 (SP) or 10 μM AZD6244 (AZ) for inhibition of p38 MAPK, JNK or MEK1/2 respectively ( n= 5 for mRNA and n= 3 for protein). ** P

    Article Snippet: Furthermore, cells were incubated with inhibitors of p38 MAPK (SB203580 [InvivoGen, UK]; 10 μM), JNK (SP600125 [InvivoGen]; 20 μM) and MEK1 (AZD6244 [Selleckchem, USA]; 10 μM) for 1 h before IL-1β stimulation.

    Techniques: Expressing, Western Blot, Concentration Assay, Recombinant, Inhibition

    A HSD exacerbates colonic cytokine expression in Il10 −/− animals (A–H) qPCR analysis of colonic mRNA from mice in Fig. 3. Data were analyzed using the ΔΔC T method against WT controls. (I–N) Bone marrow-derived dendritic cells (BMDCs) in HSM or normal salt media (NSM) were treated with 1ng/ml LPS for 8 hours before indicated cytokines were quantitated by ELISA of supernatants or, for pro-IL-1β, cell lysate (I–L) without, or with different concentrations (M) of the SGK1 inhibitor GSK 650394 or (N) the p38 MAPK inhibitor SB203580. Samples that failed quality control by melt curve analysis are omitted. P values determined by Mann Whitney nonparametric tests. Data are combined from 2 replicate experiments.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Dietary Salt Exacerbates Experimental Colitis

    doi: 10.4049/jimmunol.1700356

    Figure Lengend Snippet: A HSD exacerbates colonic cytokine expression in Il10 −/− animals (A–H) qPCR analysis of colonic mRNA from mice in Fig. 3. Data were analyzed using the ΔΔC T method against WT controls. (I–N) Bone marrow-derived dendritic cells (BMDCs) in HSM or normal salt media (NSM) were treated with 1ng/ml LPS for 8 hours before indicated cytokines were quantitated by ELISA of supernatants or, for pro-IL-1β, cell lysate (I–L) without, or with different concentrations (M) of the SGK1 inhibitor GSK 650394 or (N) the p38 MAPK inhibitor SB203580. Samples that failed quality control by melt curve analysis are omitted. P values determined by Mann Whitney nonparametric tests. Data are combined from 2 replicate experiments.

    Article Snippet: SGK1 inhibitor GSK 650394 (Tocris Biotechne); p38 MAPK inhibitor SB203580 (Invivogen)

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Mouse Assay, Derivative Assay, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

    Tacrolimus and MPA can inhibit signaling pathway activation in whole-blood samples. (A) Phospho-p38MAPK (upper left panel), p-ERK (upper right panel) and p-Akt (lower panel) phosphorylation in monocytes was measured as MFI level. Blood samples from healthy volunteers were spiked with vehicle, 10 ng/ml tacrolimus, 50 ng/ml tacrolimus, 200 ng/ml tacrolimus, 10 μg/ml MPA or 20 μM of the MAPK inhibitor SB203580. The effect of tacrolimus and MPA was based on the stimulated samples without the addition of drugs. The MAPK inhibitor was used as a positive control. Gating was performed according to Fig 2 . Tacrolimus was found to have an effect on p38MAPK, ERK and Akt phosphorylation. Akt and ERK phosphorylation was decreased in the presence of MPA. (B) Percentages of inhibition for the phosphorylation of p38MAPK (upper left panel), ERK (upper right panel) and Akt (lower panel). Data are normalized for the MFI values of the stimulated samples without the addition of immunosuppressive drugs. (Data are plotted as the mean ±SEM; n = 5) *) p

    Journal: PLoS ONE

    Article Title: The Effect of Tacrolimus and Mycophenolic Acid on CD14+ Monocyte Activation and Function

    doi: 10.1371/journal.pone.0170806

    Figure Lengend Snippet: Tacrolimus and MPA can inhibit signaling pathway activation in whole-blood samples. (A) Phospho-p38MAPK (upper left panel), p-ERK (upper right panel) and p-Akt (lower panel) phosphorylation in monocytes was measured as MFI level. Blood samples from healthy volunteers were spiked with vehicle, 10 ng/ml tacrolimus, 50 ng/ml tacrolimus, 200 ng/ml tacrolimus, 10 μg/ml MPA or 20 μM of the MAPK inhibitor SB203580. The effect of tacrolimus and MPA was based on the stimulated samples without the addition of drugs. The MAPK inhibitor was used as a positive control. Gating was performed according to Fig 2 . Tacrolimus was found to have an effect on p38MAPK, ERK and Akt phosphorylation. Akt and ERK phosphorylation was decreased in the presence of MPA. (B) Percentages of inhibition for the phosphorylation of p38MAPK (upper left panel), ERK (upper right panel) and Akt (lower panel). Data are normalized for the MFI values of the stimulated samples without the addition of immunosuppressive drugs. (Data are plotted as the mean ±SEM; n = 5) *) p

    Article Snippet: Samples were incubated for one hour at 37°C with either vehicle, tacrolimus (10, 50 or 200 ng/ml; Prograf® , Astellas Pharma Inc., Tokyo, Japan), MPA (10 μg/ml; Sigma-Aldrich, Steinheim, Germany) or the p38MAPK inhibitor SB203580 (20 μM; Invivogen, San Diego, CA).

    Techniques: Activation Assay, Positive Control, Inhibition