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Merck KGaA p38 mapk inhibitor sb202190
Targeting of Dsg3 leads to <t>p38</t> <t>MAPK-dependent</t> loss of cell cohesion. A , targeting of Dsg3 function by either incubation with AK23 or siRNA-mediated depletion of Dsg3 protein levels in HaCaT cells resulted in increased cell monolayer fragmentation in dispase-based
P38 Mapk Inhibitor Sb202190, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/p38 mapk inhibitor sb202190/product/Merck KGaA
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
p38 mapk inhibitor sb202190 - by Bioz Stars, 2021-04
86/100 stars

Images

1) Product Images from "Desmoglein 2 Compensates for Desmoglein 3 but Does Not Control Cell Adhesion via Regulation of p38 Mitogen-activated Protein Kinase in Keratinocytes"

Article Title: Desmoglein 2 Compensates for Desmoglein 3 but Does Not Control Cell Adhesion via Regulation of p38 Mitogen-activated Protein Kinase in Keratinocytes

Journal: The Journal of Biological Chemistry

doi: 10.1074/jbc.M113.489336

Targeting of Dsg3 leads to p38 MAPK-dependent loss of cell cohesion. A , targeting of Dsg3 function by either incubation with AK23 or siRNA-mediated depletion of Dsg3 protein levels in HaCaT cells resulted in increased cell monolayer fragmentation in dispase-based
Figure Legend Snippet: Targeting of Dsg3 leads to p38 MAPK-dependent loss of cell cohesion. A , targeting of Dsg3 function by either incubation with AK23 or siRNA-mediated depletion of Dsg3 protein levels in HaCaT cells resulted in increased cell monolayer fragmentation in dispase-based

Techniques Used: Incubation

p38 MAPK is activated following Dsg3 depletion and forms a complex with Dsg3. A , HaCaT cells were transfected with siRNA targeting either Dsg2 or Dsg3, and successful knockdown was proven by reduced protein levels in a Western blot analysis against non-targeting
Figure Legend Snippet: p38 MAPK is activated following Dsg3 depletion and forms a complex with Dsg3. A , HaCaT cells were transfected with siRNA targeting either Dsg2 or Dsg3, and successful knockdown was proven by reduced protein levels in a Western blot analysis against non-targeting

Techniques Used: Transfection, Western Blot

Dsg3 forms a complex with p-p38 MAPK in both the Triton X-100-soluble and Triton X-100-insoluble pool. Western blot analysis following Triton X-100-mediated cell fractionation in HaCaT cells revealed that Dsg3 was present in the soluble ( S ) and insoluble
Figure Legend Snippet: Dsg3 forms a complex with p-p38 MAPK in both the Triton X-100-soluble and Triton X-100-insoluble pool. Western blot analysis following Triton X-100-mediated cell fractionation in HaCaT cells revealed that Dsg3 was present in the soluble ( S ) and insoluble

Techniques Used: Western Blot, Cell Fractionation

Mechanisms of Dsg2- and Dsg3-mediated cell-cell adhesion in keratinocytes. Targeting of Dsg3 binding either by pemphigus antibodies or siRNA leads to p38 MAPK activation and following retraction of the keratin filament network, which both negatively affect
Figure Legend Snippet: Mechanisms of Dsg2- and Dsg3-mediated cell-cell adhesion in keratinocytes. Targeting of Dsg3 binding either by pemphigus antibodies or siRNA leads to p38 MAPK activation and following retraction of the keratin filament network, which both negatively affect

Techniques Used: Binding Assay, Activation Assay

Dsg3 depletion recruits Dsg2 and p-p38 MAPK to the plasma membrane, accompanied by alterations in PG protein and Dsg2 mRNA expression. A , representative Western blot analysis with detection of Dsg2 and PG in total cell lysates of MEK cells 24 h after
Figure Legend Snippet: Dsg3 depletion recruits Dsg2 and p-p38 MAPK to the plasma membrane, accompanied by alterations in PG protein and Dsg2 mRNA expression. A , representative Western blot analysis with detection of Dsg2 and PG in total cell lysates of MEK cells 24 h after

Techniques Used: Expressing, Western Blot

2) Product Images from "Desmoglein 2 Compensates for Desmoglein 3 but Does Not Control Cell Adhesion via Regulation of p38 Mitogen-activated Protein Kinase in Keratinocytes"

Article Title: Desmoglein 2 Compensates for Desmoglein 3 but Does Not Control Cell Adhesion via Regulation of p38 Mitogen-activated Protein Kinase in Keratinocytes

Journal: The Journal of Biological Chemistry

doi: 10.1074/jbc.M113.489336

Targeting of Dsg3 leads to p38 MAPK-dependent loss of cell cohesion. A , targeting of Dsg3 function by either incubation with AK23 or siRNA-mediated depletion of Dsg3 protein levels in HaCaT cells resulted in increased cell monolayer fragmentation in dispase-based
Figure Legend Snippet: Targeting of Dsg3 leads to p38 MAPK-dependent loss of cell cohesion. A , targeting of Dsg3 function by either incubation with AK23 or siRNA-mediated depletion of Dsg3 protein levels in HaCaT cells resulted in increased cell monolayer fragmentation in dispase-based

Techniques Used: Incubation

p38 MAPK is activated following Dsg3 depletion and forms a complex with Dsg3. A , HaCaT cells were transfected with siRNA targeting either Dsg2 or Dsg3, and successful knockdown was proven by reduced protein levels in a Western blot analysis against non-targeting
Figure Legend Snippet: p38 MAPK is activated following Dsg3 depletion and forms a complex with Dsg3. A , HaCaT cells were transfected with siRNA targeting either Dsg2 or Dsg3, and successful knockdown was proven by reduced protein levels in a Western blot analysis against non-targeting

Techniques Used: Transfection, Western Blot

Dsg3 forms a complex with p-p38 MAPK in both the Triton X-100-soluble and Triton X-100-insoluble pool. Western blot analysis following Triton X-100-mediated cell fractionation in HaCaT cells revealed that Dsg3 was present in the soluble ( S ) and insoluble
Figure Legend Snippet: Dsg3 forms a complex with p-p38 MAPK in both the Triton X-100-soluble and Triton X-100-insoluble pool. Western blot analysis following Triton X-100-mediated cell fractionation in HaCaT cells revealed that Dsg3 was present in the soluble ( S ) and insoluble

Techniques Used: Western Blot, Cell Fractionation

Mechanisms of Dsg2- and Dsg3-mediated cell-cell adhesion in keratinocytes. Targeting of Dsg3 binding either by pemphigus antibodies or siRNA leads to p38 MAPK activation and following retraction of the keratin filament network, which both negatively affect
Figure Legend Snippet: Mechanisms of Dsg2- and Dsg3-mediated cell-cell adhesion in keratinocytes. Targeting of Dsg3 binding either by pemphigus antibodies or siRNA leads to p38 MAPK activation and following retraction of the keratin filament network, which both negatively affect

Techniques Used: Binding Assay, Activation Assay

Dsg3 depletion recruits Dsg2 and p-p38 MAPK to the plasma membrane, accompanied by alterations in PG protein and Dsg2 mRNA expression. A , representative Western blot analysis with detection of Dsg2 and PG in total cell lysates of MEK cells 24 h after
Figure Legend Snippet: Dsg3 depletion recruits Dsg2 and p-p38 MAPK to the plasma membrane, accompanied by alterations in PG protein and Dsg2 mRNA expression. A , representative Western blot analysis with detection of Dsg2 and PG in total cell lysates of MEK cells 24 h after

Techniques Used: Expressing, Western Blot

Related Articles

other:

Article Title: Avian Reovirus Nonstructural Protein p17-Induced G2/M Cell Cycle Arrest and Host Cellular Protein Translation Shutoff Involve Activation of p53-Dependent Pathways ▿
Article Snippet: The p38 MAPK inhibitor SB202190 and the phosphatidylinositol 3-kinase (PI3K) inhibitor wortmannin were from Merck (Frankfurter, Darmstadt, Germany).

Article Title: Involvement of MAPK signalling in radioadaptive response in BALB/c mice exposed to low dose ionizing radiation.
Article Snippet: To investigate low dose ionizing radiation (LDIR)-induced adaptive response in lymphocytes of BALB/c mice and to elucidate related molecular mechanisms.

Article Title: Inflammatory tumour cell killing by oncolytic reovirus for the treatment of melanoma
Article Snippet: MEK1/2 inhibitor U0126 (1,4-Diamino-2,3-dicyano-1,4-bis(2-aminophenylthio) butadiene), PI3K inhibitor LY294002 (2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one) and p38 MAPK inhibitor SB202190 (4-(4-fluorophenyl)-2-(4-hydroxyphenyl)-5-(4-pyridyl)1H-imidazole) were all obtained from Merck Biosciences, UK.

Concentration Assay:

Article Title: Desmoglein 2 Compensates for Desmoglein 3 but Does Not Control Cell Adhesion via Regulation of p38 Mitogen-activated Protein Kinase in Keratinocytes
Article Snippet: AK23 (host, mouse; isotype, IgG) is a monoclonal antibody targeting Dsg3 (Biozol, Eching, Germany) and was utilized for the incubation steps in the cell culture model at a concentration of 75 μg/ml. .. The specific p38 MAPK inhibitor SB202190 (Merck, Darmstadt, Germany) was applied at a concentration of 30 μmol/liter for 24 h either alone or 1 h before AK23 incubation started. .. One-day-old littermates of heterozygous B6;129X1-Dsg3tm1Stan /J mice ( ) (The Jackson Laboratory, Bar Harbor, ME) were used for the isolation of primary murine keratinocytes.

Incubation:

Article Title: Desmoglein 2 Compensates for Desmoglein 3 but Does Not Control Cell Adhesion via Regulation of p38 Mitogen-activated Protein Kinase in Keratinocytes
Article Snippet: AK23 (host, mouse; isotype, IgG) is a monoclonal antibody targeting Dsg3 (Biozol, Eching, Germany) and was utilized for the incubation steps in the cell culture model at a concentration of 75 μg/ml. .. The specific p38 MAPK inhibitor SB202190 (Merck, Darmstadt, Germany) was applied at a concentration of 30 μmol/liter for 24 h either alone or 1 h before AK23 incubation started. .. One-day-old littermates of heterozygous B6;129X1-Dsg3tm1Stan /J mice ( ) (The Jackson Laboratory, Bar Harbor, ME) were used for the isolation of primary murine keratinocytes.

Article Title: Desmoglein 2 Compensates for Desmoglein 3 but Does Not Control Cell Adhesion via Regulation of p38 Mitogen-activated Protein Kinase in Keratinocytes
Article Snippet: Both targeting Dsg3 with AK23 (fragment number 24.0 ± 2.5) or by siRNA (12.5 ± 2.7) resulted in a loss of cell cohesion compared with cells transfected with non-targeting siRNA (2.0 ± 0.3). .. Strikingly, 1 h of preincubation with the p38 MAPK inhibitor SB202190 (3.4 ± 1.2) or 24-h incubation of Dsg3-depleted cells with SB202190 significantly reduced the number of fragments (5.6 ± 1.5). ..

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    Merck KGaA p38 mapk inhibitor sb202190
    Targeting of Dsg3 leads to <t>p38</t> <t>MAPK-dependent</t> loss of cell cohesion. A , targeting of Dsg3 function by either incubation with AK23 or siRNA-mediated depletion of Dsg3 protein levels in HaCaT cells resulted in increased cell monolayer fragmentation in dispase-based
    P38 Mapk Inhibitor Sb202190, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p38 mapk inhibitor sb202190/product/Merck KGaA
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    p38 mapk inhibitor sb202190 - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    86
    Merck KGaA sb202190
    Targeting of Dsg3 leads to <t>p38</t> <t>MAPK-dependent</t> loss of cell cohesion. A , targeting of Dsg3 function by either incubation with AK23 or siRNA-mediated depletion of Dsg3 protein levels in HaCaT cells resulted in increased cell monolayer fragmentation in dispase-based
    Sb202190, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sb202190/product/Merck KGaA
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sb202190 - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    Image Search Results


    Targeting of Dsg3 leads to p38 MAPK-dependent loss of cell cohesion. A , targeting of Dsg3 function by either incubation with AK23 or siRNA-mediated depletion of Dsg3 protein levels in HaCaT cells resulted in increased cell monolayer fragmentation in dispase-based

    Journal: The Journal of Biological Chemistry

    Article Title: Desmoglein 2 Compensates for Desmoglein 3 but Does Not Control Cell Adhesion via Regulation of p38 Mitogen-activated Protein Kinase in Keratinocytes

    doi: 10.1074/jbc.M113.489336

    Figure Lengend Snippet: Targeting of Dsg3 leads to p38 MAPK-dependent loss of cell cohesion. A , targeting of Dsg3 function by either incubation with AK23 or siRNA-mediated depletion of Dsg3 protein levels in HaCaT cells resulted in increased cell monolayer fragmentation in dispase-based

    Article Snippet: Strikingly, 1 h of preincubation with the p38 MAPK inhibitor SB202190 (3.4 ± 1.2) or 24-h incubation of Dsg3-depleted cells with SB202190 significantly reduced the number of fragments (5.6 ± 1.5).

    Techniques: Incubation

    p38 MAPK is activated following Dsg3 depletion and forms a complex with Dsg3. A , HaCaT cells were transfected with siRNA targeting either Dsg2 or Dsg3, and successful knockdown was proven by reduced protein levels in a Western blot analysis against non-targeting

    Journal: The Journal of Biological Chemistry

    Article Title: Desmoglein 2 Compensates for Desmoglein 3 but Does Not Control Cell Adhesion via Regulation of p38 Mitogen-activated Protein Kinase in Keratinocytes

    doi: 10.1074/jbc.M113.489336

    Figure Lengend Snippet: p38 MAPK is activated following Dsg3 depletion and forms a complex with Dsg3. A , HaCaT cells were transfected with siRNA targeting either Dsg2 or Dsg3, and successful knockdown was proven by reduced protein levels in a Western blot analysis against non-targeting

    Article Snippet: Strikingly, 1 h of preincubation with the p38 MAPK inhibitor SB202190 (3.4 ± 1.2) or 24-h incubation of Dsg3-depleted cells with SB202190 significantly reduced the number of fragments (5.6 ± 1.5).

    Techniques: Transfection, Western Blot

    Dsg3 forms a complex with p-p38 MAPK in both the Triton X-100-soluble and Triton X-100-insoluble pool. Western blot analysis following Triton X-100-mediated cell fractionation in HaCaT cells revealed that Dsg3 was present in the soluble ( S ) and insoluble

    Journal: The Journal of Biological Chemistry

    Article Title: Desmoglein 2 Compensates for Desmoglein 3 but Does Not Control Cell Adhesion via Regulation of p38 Mitogen-activated Protein Kinase in Keratinocytes

    doi: 10.1074/jbc.M113.489336

    Figure Lengend Snippet: Dsg3 forms a complex with p-p38 MAPK in both the Triton X-100-soluble and Triton X-100-insoluble pool. Western blot analysis following Triton X-100-mediated cell fractionation in HaCaT cells revealed that Dsg3 was present in the soluble ( S ) and insoluble

    Article Snippet: Strikingly, 1 h of preincubation with the p38 MAPK inhibitor SB202190 (3.4 ± 1.2) or 24-h incubation of Dsg3-depleted cells with SB202190 significantly reduced the number of fragments (5.6 ± 1.5).

    Techniques: Western Blot, Cell Fractionation

    Mechanisms of Dsg2- and Dsg3-mediated cell-cell adhesion in keratinocytes. Targeting of Dsg3 binding either by pemphigus antibodies or siRNA leads to p38 MAPK activation and following retraction of the keratin filament network, which both negatively affect

    Journal: The Journal of Biological Chemistry

    Article Title: Desmoglein 2 Compensates for Desmoglein 3 but Does Not Control Cell Adhesion via Regulation of p38 Mitogen-activated Protein Kinase in Keratinocytes

    doi: 10.1074/jbc.M113.489336

    Figure Lengend Snippet: Mechanisms of Dsg2- and Dsg3-mediated cell-cell adhesion in keratinocytes. Targeting of Dsg3 binding either by pemphigus antibodies or siRNA leads to p38 MAPK activation and following retraction of the keratin filament network, which both negatively affect

    Article Snippet: Strikingly, 1 h of preincubation with the p38 MAPK inhibitor SB202190 (3.4 ± 1.2) or 24-h incubation of Dsg3-depleted cells with SB202190 significantly reduced the number of fragments (5.6 ± 1.5).

    Techniques: Binding Assay, Activation Assay

    Dsg3 depletion recruits Dsg2 and p-p38 MAPK to the plasma membrane, accompanied by alterations in PG protein and Dsg2 mRNA expression. A , representative Western blot analysis with detection of Dsg2 and PG in total cell lysates of MEK cells 24 h after

    Journal: The Journal of Biological Chemistry

    Article Title: Desmoglein 2 Compensates for Desmoglein 3 but Does Not Control Cell Adhesion via Regulation of p38 Mitogen-activated Protein Kinase in Keratinocytes

    doi: 10.1074/jbc.M113.489336

    Figure Lengend Snippet: Dsg3 depletion recruits Dsg2 and p-p38 MAPK to the plasma membrane, accompanied by alterations in PG protein and Dsg2 mRNA expression. A , representative Western blot analysis with detection of Dsg2 and PG in total cell lysates of MEK cells 24 h after

    Article Snippet: Strikingly, 1 h of preincubation with the p38 MAPK inhibitor SB202190 (3.4 ± 1.2) or 24-h incubation of Dsg3-depleted cells with SB202190 significantly reduced the number of fragments (5.6 ± 1.5).

    Techniques: Expressing, Western Blot