p38 agonist anisomycin  (Cell Signaling Technology Inc)

Journal: BioMed Research International

Article Title: Effect of TRPV4-p38 MAPK Pathway on Neuropathic Pain in Rats with Chronic Compression of the Dorsal Root Ganglion

doi: 10.1155/2016/6978923

Figure Lengend Snippet: The effects of the reagents on CCD-induced mechanical allodynia. (a–d) The PWMTs of CCD rats (4 days after operation) 1, 2, 4, and 8 h after RR, 4 α -PDD, SB203580, and anisomycin intrathecal injections ( n = 6; the data are expressed as means ± SEMs); ∗ P < 0.05 and ∗∗ P < 0.01 compared ipsilaterally with the saline group; one-way ANOVA followed by Tukey's post hoc test.

Article Snippet: Four days after CCD surgery, the TRPV4 inhibitor Ruthenium Red (RR, Sigma, Germany), the TRPV4 agonist 4 α -PDD (CST, USA), the p38 inhibitor SB203580 (CST, USA), or the p38 agonist anisomycin (CST, USA) was given to the experimental groups at the recommended concentrations via intrathecal injection.

Techniques:

Journal: BioMed Research International

Article Title: Effect of TRPV4-p38 MAPK Pathway on Neuropathic Pain in Rats with Chronic Compression of the Dorsal Root Ganglion

doi: 10.1155/2016/6978923

Figure Lengend Snippet: Distribution changes of the p38-positive neurons in DRG tissue. (a–f) p38 immunohistochemical staining of the DRG neurons in the control, CCD, CCD+RR 10 nmol/L, CCD+4 α -PDD 10 nmol/L, CCD+SB203580 20 μ mol/L, and CCD+anisomycin 25 μ g/mL groups. Scale bars: 100 μ m. (g) The analysis of the p38-positive neurons (shown as means ± SEMs); ∗ P < 0.05 and ∗∗ P < 0.01 compared with controls; ## P < 0.01 compared with the CCD group.

Article Snippet: Four days after CCD surgery, the TRPV4 inhibitor Ruthenium Red (RR, Sigma, Germany), the TRPV4 agonist 4 α -PDD (CST, USA), the p38 inhibitor SB203580 (CST, USA), or the p38 agonist anisomycin (CST, USA) was given to the experimental groups at the recommended concentrations via intrathecal injection.

Techniques: Immunohistochemical staining, Staining

Journal: BioMed Research International

Article Title: Effect of TRPV4-p38 MAPK Pathway on Neuropathic Pain in Rats with Chronic Compression of the Dorsal Root Ganglion

doi: 10.1155/2016/6978923

Figure Lengend Snippet: The effect of reagents on the protein expression of TRPV4, p38, and P-p38. (a–d) The expression of TRPV4, p38, and P-p38 proteins after RR, 4 α -PDD, SB203580, and anisomycin intrathecal injections given to rats 4 days after CCD surgery. The bars represent means ± SEMs based on six experiments; ∗ P < 0.05 and ∗∗ P < 0.01, TRPV4 compared with controls. # P < 0.05 and ## P < 0.01, p38 compared with controls. & P < 0.05 and && P < 0.01, P-p38 compared with controls.

Article Snippet: Four days after CCD surgery, the TRPV4 inhibitor Ruthenium Red (RR, Sigma, Germany), the TRPV4 agonist 4 α -PDD (CST, USA), the p38 inhibitor SB203580 (CST, USA), or the p38 agonist anisomycin (CST, USA) was given to the experimental groups at the recommended concentrations via intrathecal injection.

Techniques: Expressing

Journal: BioMed Research International

Article Title: Effect of TRPV4-p38 MAPK Pathway on Neuropathic Pain in Rats with Chronic Compression of the Dorsal Root Ganglion

doi: 10.1155/2016/6978923

Figure Lengend Snippet: Altered distribution of TRPV4-positive neurons in DRG tissue. (a–f) TRPV4 immunohistochemical staining of the DRG neurons in the control, CCD, CCD+RR 10 nmol/L, CCD+4 α -PDD 10 nmol/L, CCD+SB203580 20 μ mol/L, and CCD+anisomycin 25 μ g/mL groups, respectively. Scale bars: 100 μ m. (g) The analysis of the TRPV4-positive neurons (shown as means ± SEMs); ∗∗ P < 0.01 compared with controls; ## P < 0.01 compared with the CCD group.

Article Snippet: Four days after CCD surgery, the TRPV4 inhibitor Ruthenium Red (RR, Sigma, Germany), the TRPV4 agonist 4 α -PDD (CST, USA), the p38 inhibitor SB203580 (CST, USA), or the p38 agonist anisomycin (CST, USA) was given to the experimental groups at the recommended concentrations via intrathecal injection.

Techniques: Immunohistochemical staining, Staining

Journal: BioMed Research International

Article Title: Effect of TRPV4-p38 MAPK Pathway on Neuropathic Pain in Rats with Chronic Compression of the Dorsal Root Ganglion

doi: 10.1155/2016/6978923

Figure Lengend Snippet: Ectopic discharges after CCD surgery and reagent injection. (a–f) represent discharges of the control, CCD, CCD+RR 10 nmol/L, CCD+4 α -PDD 10 nmol/L, CCD+SB203580 20 μ mol/L, and CCD+anisomycin 25 μ g/mL groups. Cut from BL-420E+ biological and functional experimental system. (g) and (h) show the amplitudes and frequencies for the different groups (data are expressed as means ± SEM); ∗∗ P < 0.01, compared with the CCD group (7-8 rats in each group).

Article Snippet: Four days after CCD surgery, the TRPV4 inhibitor Ruthenium Red (RR, Sigma, Germany), the TRPV4 agonist 4 α -PDD (CST, USA), the p38 inhibitor SB203580 (CST, USA), or the p38 agonist anisomycin (CST, USA) was given to the experimental groups at the recommended concentrations via intrathecal injection.

Techniques: Injection, Functional Assay