p2y4  (Alomone Labs)


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    Structured Review

    Alomone Labs p2y4
    Subcellular distribution of P2Y2, <t>P2Y4,</t> and P2Y6 receptors in MEFs. WT or KI MEFs were cultured and, after changing the medium, were maintained in the absence or presence of 1 μ M DFU for 2 h. Cells were fixed with paraformaldehyde (4%; pH 7.2) and permeabilized with cold methanol at RT. After incubation with anti-P2Y2, anti-P2Y4 and anti-P2Y6 antibodies (1 : 500) overnight at 4°C, cells were visualized by confocal microscopy using a FITC-conjugated secondary Ab (Alexa-Fluor 488, 1 : 1000). Nuclei were stained with Hoechst 33258. Coverslips were mounted in Prolong Gold antifade reagent (Molecular Probes) and the intensity of the fluorescence was measured using Image J software (NIH, Bethesda, MD, USA). Results show the mean + SD of three experiments. ** P
    P2y4, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 88/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p2y4/product/Alomone Labs
    Average 88 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    p2y4 - by Bioz Stars, 2022-09
    88/100 stars

    Images

    1) Product Images from "Sustained Release of Prostaglandin E2 in Fibroblasts Expressing Ectopically Cyclooxygenase 2 Impairs P2Y-Dependent Ca2+-Mobilization"

    Article Title: Sustained Release of Prostaglandin E2 in Fibroblasts Expressing Ectopically Cyclooxygenase 2 Impairs P2Y-Dependent Ca2+-Mobilization

    Journal: Mediators of Inflammation

    doi: 10.1155/2014/832103

    Subcellular distribution of P2Y2, P2Y4, and P2Y6 receptors in MEFs. WT or KI MEFs were cultured and, after changing the medium, were maintained in the absence or presence of 1 μ M DFU for 2 h. Cells were fixed with paraformaldehyde (4%; pH 7.2) and permeabilized with cold methanol at RT. After incubation with anti-P2Y2, anti-P2Y4 and anti-P2Y6 antibodies (1 : 500) overnight at 4°C, cells were visualized by confocal microscopy using a FITC-conjugated secondary Ab (Alexa-Fluor 488, 1 : 1000). Nuclei were stained with Hoechst 33258. Coverslips were mounted in Prolong Gold antifade reagent (Molecular Probes) and the intensity of the fluorescence was measured using Image J software (NIH, Bethesda, MD, USA). Results show the mean + SD of three experiments. ** P
    Figure Legend Snippet: Subcellular distribution of P2Y2, P2Y4, and P2Y6 receptors in MEFs. WT or KI MEFs were cultured and, after changing the medium, were maintained in the absence or presence of 1 μ M DFU for 2 h. Cells were fixed with paraformaldehyde (4%; pH 7.2) and permeabilized with cold methanol at RT. After incubation with anti-P2Y2, anti-P2Y4 and anti-P2Y6 antibodies (1 : 500) overnight at 4°C, cells were visualized by confocal microscopy using a FITC-conjugated secondary Ab (Alexa-Fluor 488, 1 : 1000). Nuclei were stained with Hoechst 33258. Coverslips were mounted in Prolong Gold antifade reagent (Molecular Probes) and the intensity of the fluorescence was measured using Image J software (NIH, Bethesda, MD, USA). Results show the mean + SD of three experiments. ** P

    Techniques Used: Cell Culture, Incubation, Confocal Microscopy, Staining, Fluorescence, Software

    Characterization of EP1–4 and P2Y2–P2Y4 expression and effect of ionophores on Ca 2+ -mobilization in MEF cells. The expression levels of the prostaglandin receptors EP1–4 and the levels of P2Y2 and P2Y4 were determined by qPCR (a-b). The response to 1 μ M ionomycin (c) and 500 nM thapsigargin (d) on Ca 2+ -mobilization was determined in MEFs overexpressing COX-2, using the dual excitation 340/380 nm protocol as described in Section 2 . MEFs KI were washed with fresh medium to remove PGE 2 accumulated and maintained in the absence or presence of 1 μ M DFU and 5 μ M PGE 2 . Different extracellular concentrations of calcium were used. Results show the mean + SD of three experiments (a-b) or a representative trace (c-d). * P
    Figure Legend Snippet: Characterization of EP1–4 and P2Y2–P2Y4 expression and effect of ionophores on Ca 2+ -mobilization in MEF cells. The expression levels of the prostaglandin receptors EP1–4 and the levels of P2Y2 and P2Y4 were determined by qPCR (a-b). The response to 1 μ M ionomycin (c) and 500 nM thapsigargin (d) on Ca 2+ -mobilization was determined in MEFs overexpressing COX-2, using the dual excitation 340/380 nm protocol as described in Section 2 . MEFs KI were washed with fresh medium to remove PGE 2 accumulated and maintained in the absence or presence of 1 μ M DFU and 5 μ M PGE 2 . Different extracellular concentrations of calcium were used. Results show the mean + SD of three experiments (a-b) or a representative trace (c-d). * P

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction

    2) Product Images from "Evidence for the Expression of Multiple Uracil Nucleotide-Stimulated P2 Receptors Coupled to Smooth Muscle Contraction in Porcine Isolated Arteries"

    Article Title: Evidence for the Expression of Multiple Uracil Nucleotide-Stimulated P2 Receptors Coupled to Smooth Muscle Contraction in Porcine Isolated Arteries

    Journal:

    doi: 10.1038/sj.bjp.0707120

    RT-PCR and sequence analysis of porcine P2Y2 , P2Y4 and P2Y6 receptors
    Figure Legend Snippet: RT-PCR and sequence analysis of porcine P2Y2 , P2Y4 and P2Y6 receptors

    Techniques Used: Reverse Transcription Polymerase Chain Reaction, Sequencing

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    Alomone Labs p2y4
    Subcellular distribution of P2Y2, <t>P2Y4,</t> and P2Y6 receptors in MEFs. WT or KI MEFs were cultured and, after changing the medium, were maintained in the absence or presence of 1 μ M DFU for 2 h. Cells were fixed with paraformaldehyde (4%; pH 7.2) and permeabilized with cold methanol at RT. After incubation with anti-P2Y2, anti-P2Y4 and anti-P2Y6 antibodies (1 : 500) overnight at 4°C, cells were visualized by confocal microscopy using a FITC-conjugated secondary Ab (Alexa-Fluor 488, 1 : 1000). Nuclei were stained with Hoechst 33258. Coverslips were mounted in Prolong Gold antifade reagent (Molecular Probes) and the intensity of the fluorescence was measured using Image J software (NIH, Bethesda, MD, USA). Results show the mean + SD of three experiments. ** P
    P2y4, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p2y4/product/Alomone Labs
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    p2y4 - by Bioz Stars, 2022-09
    93/100 stars
      Buy from Supplier

    88
    Alomone Labs p2y4 receptors
    Representative traces of [Ca 2+ ] i response in solitary neurons and astrocytes in low density cultures stimulated with 30 μM UTP (A); 100 μM ATP (B). The traces shown are from a single neuron and astrocyte representing 15 neurons and 40 astrocytes from eight experiments. (C and D) Confocal images of mixed hippocampal cultures immunostained with anti-P2Y receptors (green) and synaptophysin (red). P2Y2 and <t>P2Y4</t> receptors are expressed only in astrocytes. P2Y2 and P2Y4 receptor immunofluorescence was not colocalized with synaptophysin fluorescence at synaptic terminals.
    P2y4 Receptors, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p2y4 receptors/product/Alomone Labs
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    p2y4 receptors - by Bioz Stars, 2022-09
    88/100 stars
      Buy from Supplier

    Image Search Results


    Subcellular distribution of P2Y2, P2Y4, and P2Y6 receptors in MEFs. WT or KI MEFs were cultured and, after changing the medium, were maintained in the absence or presence of 1 μ M DFU for 2 h. Cells were fixed with paraformaldehyde (4%; pH 7.2) and permeabilized with cold methanol at RT. After incubation with anti-P2Y2, anti-P2Y4 and anti-P2Y6 antibodies (1 : 500) overnight at 4°C, cells were visualized by confocal microscopy using a FITC-conjugated secondary Ab (Alexa-Fluor 488, 1 : 1000). Nuclei were stained with Hoechst 33258. Coverslips were mounted in Prolong Gold antifade reagent (Molecular Probes) and the intensity of the fluorescence was measured using Image J software (NIH, Bethesda, MD, USA). Results show the mean + SD of three experiments. ** P

    Journal: Mediators of Inflammation

    Article Title: Sustained Release of Prostaglandin E2 in Fibroblasts Expressing Ectopically Cyclooxygenase 2 Impairs P2Y-Dependent Ca2+-Mobilization

    doi: 10.1155/2014/832103

    Figure Lengend Snippet: Subcellular distribution of P2Y2, P2Y4, and P2Y6 receptors in MEFs. WT or KI MEFs were cultured and, after changing the medium, were maintained in the absence or presence of 1 μ M DFU for 2 h. Cells were fixed with paraformaldehyde (4%; pH 7.2) and permeabilized with cold methanol at RT. After incubation with anti-P2Y2, anti-P2Y4 and anti-P2Y6 antibodies (1 : 500) overnight at 4°C, cells were visualized by confocal microscopy using a FITC-conjugated secondary Ab (Alexa-Fluor 488, 1 : 1000). Nuclei were stained with Hoechst 33258. Coverslips were mounted in Prolong Gold antifade reagent (Molecular Probes) and the intensity of the fluorescence was measured using Image J software (NIH, Bethesda, MD, USA). Results show the mean + SD of three experiments. ** P

    Article Snippet: Antibodies against P2Y2, P2Y4, and P2Y6 receptors were from Alomone Labs (Jerusalem, Israel) and other antibodies were from Santa Cruz Biotech (Santa Cruz, CA, USA), from Cell Signaling (Danvers, MA, USA), or from the sources previously described [ ].

    Techniques: Cell Culture, Incubation, Confocal Microscopy, Staining, Fluorescence, Software

    Characterization of EP1–4 and P2Y2–P2Y4 expression and effect of ionophores on Ca 2+ -mobilization in MEF cells. The expression levels of the prostaglandin receptors EP1–4 and the levels of P2Y2 and P2Y4 were determined by qPCR (a-b). The response to 1 μ M ionomycin (c) and 500 nM thapsigargin (d) on Ca 2+ -mobilization was determined in MEFs overexpressing COX-2, using the dual excitation 340/380 nm protocol as described in Section 2 . MEFs KI were washed with fresh medium to remove PGE 2 accumulated and maintained in the absence or presence of 1 μ M DFU and 5 μ M PGE 2 . Different extracellular concentrations of calcium were used. Results show the mean + SD of three experiments (a-b) or a representative trace (c-d). * P

    Journal: Mediators of Inflammation

    Article Title: Sustained Release of Prostaglandin E2 in Fibroblasts Expressing Ectopically Cyclooxygenase 2 Impairs P2Y-Dependent Ca2+-Mobilization

    doi: 10.1155/2014/832103

    Figure Lengend Snippet: Characterization of EP1–4 and P2Y2–P2Y4 expression and effect of ionophores on Ca 2+ -mobilization in MEF cells. The expression levels of the prostaglandin receptors EP1–4 and the levels of P2Y2 and P2Y4 were determined by qPCR (a-b). The response to 1 μ M ionomycin (c) and 500 nM thapsigargin (d) on Ca 2+ -mobilization was determined in MEFs overexpressing COX-2, using the dual excitation 340/380 nm protocol as described in Section 2 . MEFs KI were washed with fresh medium to remove PGE 2 accumulated and maintained in the absence or presence of 1 μ M DFU and 5 μ M PGE 2 . Different extracellular concentrations of calcium were used. Results show the mean + SD of three experiments (a-b) or a representative trace (c-d). * P

    Article Snippet: Antibodies against P2Y2, P2Y4, and P2Y6 receptors were from Alomone Labs (Jerusalem, Israel) and other antibodies were from Santa Cruz Biotech (Santa Cruz, CA, USA), from Cell Signaling (Danvers, MA, USA), or from the sources previously described [ ].

    Techniques: Expressing, Real-time Polymerase Chain Reaction

    Expression of P2Y4 receptors in recurrent laryngeal nerve. Immunofluorescence staining for Schwann cell maker P75 NGFR (P75) or S100, and axonal neurofilament (NF). Nuclei were counterstained with DAPI. A. Weak immunopositive signal of P2Y4 (red) was located

    Journal: International Journal of Clinical and Experimental Medicine

    Article Title: Expression of purinergic receptor P2Y4 in Schwann cell following nerve regeneration

    doi:

    Figure Lengend Snippet: Expression of P2Y4 receptors in recurrent laryngeal nerve. Immunofluorescence staining for Schwann cell maker P75 NGFR (P75) or S100, and axonal neurofilament (NF). Nuclei were counterstained with DAPI. A. Weak immunopositive signal of P2Y4 (red) was located

    Article Snippet: The primary rabbit polyclonal P2Y4 antibody (APR-006, 1:100) was purchased from Alomone Labs Ltd.

    Techniques: Expressing, Immunofluorescence, Staining

    Western blot for detecting protein expression level of P2Y4 receptor in recurrent laryngeal nerve. The measurement was repeated at least 3 times. Control, 4 d, 7 d, 14 d and 30 d lanes represent the expression of P2Y4 receptor and GAPDH in the control

    Journal: International Journal of Clinical and Experimental Medicine

    Article Title: Expression of purinergic receptor P2Y4 in Schwann cell following nerve regeneration

    doi:

    Figure Lengend Snippet: Western blot for detecting protein expression level of P2Y4 receptor in recurrent laryngeal nerve. The measurement was repeated at least 3 times. Control, 4 d, 7 d, 14 d and 30 d lanes represent the expression of P2Y4 receptor and GAPDH in the control

    Article Snippet: The primary rabbit polyclonal P2Y4 antibody (APR-006, 1:100) was purchased from Alomone Labs Ltd.

    Techniques: Western Blot, Expressing

    Real-time qPCR for detecting mRNA expression level of P2Y4 receptor in recurrent laryngeal nerve. The quantitative assessment showed that transcription expression level of P2Y4 receptors was significantly up-regulated after injury, and peaked on the post-injury

    Journal: International Journal of Clinical and Experimental Medicine

    Article Title: Expression of purinergic receptor P2Y4 in Schwann cell following nerve regeneration

    doi:

    Figure Lengend Snippet: Real-time qPCR for detecting mRNA expression level of P2Y4 receptor in recurrent laryngeal nerve. The quantitative assessment showed that transcription expression level of P2Y4 receptors was significantly up-regulated after injury, and peaked on the post-injury

    Article Snippet: The primary rabbit polyclonal P2Y4 antibody (APR-006, 1:100) was purchased from Alomone Labs Ltd.

    Techniques: Real-time Polymerase Chain Reaction, Expressing

    Representative traces of [Ca 2+ ] i response in solitary neurons and astrocytes in low density cultures stimulated with 30 μM UTP (A); 100 μM ATP (B). The traces shown are from a single neuron and astrocyte representing 15 neurons and 40 astrocytes from eight experiments. (C and D) Confocal images of mixed hippocampal cultures immunostained with anti-P2Y receptors (green) and synaptophysin (red). P2Y2 and P2Y4 receptors are expressed only in astrocytes. P2Y2 and P2Y4 receptor immunofluorescence was not colocalized with synaptophysin fluorescence at synaptic terminals.

    Journal: The Journal of General Physiology

    Article Title: Nitric Oxide-mediated Modulation of Synaptic Activity by Astrocytic P2Y Receptors

    doi: 10.1085/jgp.200810043

    Figure Lengend Snippet: Representative traces of [Ca 2+ ] i response in solitary neurons and astrocytes in low density cultures stimulated with 30 μM UTP (A); 100 μM ATP (B). The traces shown are from a single neuron and astrocyte representing 15 neurons and 40 astrocytes from eight experiments. (C and D) Confocal images of mixed hippocampal cultures immunostained with anti-P2Y receptors (green) and synaptophysin (red). P2Y2 and P2Y4 receptors are expressed only in astrocytes. P2Y2 and P2Y4 receptor immunofluorescence was not colocalized with synaptophysin fluorescence at synaptic terminals.

    Article Snippet: P2Y2 and P2Y4 receptors were profusely expressed in astrocytes but no immunofluorescence was seen in neurons ( ).

    Techniques: Immunofluorescence, Fluorescence

    Confocal images of mixed hippocampal cultures immunostained with anti-P2Y receptors (green) and neuronal markers anti-MAP-2 (red). Both P2Y2 and P2Y4 receptor immunostaining is not seen in neuronal cell bodies or processes but astrocytes are profusely stained. In merged images, some pixels appear yellow because of the neurons (red) overlying the brightly stained (green) astrocytes.

    Journal: The Journal of General Physiology

    Article Title: Nitric Oxide-mediated Modulation of Synaptic Activity by Astrocytic P2Y Receptors

    doi: 10.1085/jgp.200810043

    Figure Lengend Snippet: Confocal images of mixed hippocampal cultures immunostained with anti-P2Y receptors (green) and neuronal markers anti-MAP-2 (red). Both P2Y2 and P2Y4 receptor immunostaining is not seen in neuronal cell bodies or processes but astrocytes are profusely stained. In merged images, some pixels appear yellow because of the neurons (red) overlying the brightly stained (green) astrocytes.

    Article Snippet: P2Y2 and P2Y4 receptors were profusely expressed in astrocytes but no immunofluorescence was seen in neurons ( ).

    Techniques: Immunostaining, Staining