P2y4, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 88/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
Average 88 stars, based on 9 article reviews
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1) Product Images from "Sustained Release of Prostaglandin E2 in Fibroblasts Expressing Ectopically Cyclooxygenase 2 Impairs P2Y-Dependent Ca2+-Mobilization"
Article Title: Sustained Release of Prostaglandin E2 in Fibroblasts Expressing Ectopically Cyclooxygenase 2 Impairs P2Y-Dependent Ca2+-Mobilization
Journal: Mediators of Inflammation
Figure Legend Snippet: Subcellular distribution of P2Y2, P2Y4, and P2Y6 receptors in MEFs. WT or KI MEFs were cultured and, after changing the medium, were maintained in the absence or presence of 1 μ M DFU for 2 h. Cells were fixed with paraformaldehyde (4%; pH 7.2) and permeabilized with cold methanol at RT. After incubation with anti-P2Y2, anti-P2Y4 and anti-P2Y6 antibodies (1 : 500) overnight at 4°C, cells were visualized by confocal microscopy using a FITC-conjugated secondary Ab (Alexa-Fluor 488, 1 : 1000). Nuclei were stained with Hoechst 33258. Coverslips were mounted in Prolong Gold antifade reagent (Molecular Probes) and the intensity of the fluorescence was measured using Image J software (NIH, Bethesda, MD, USA). Results show the mean + SD of three experiments. ** P
Techniques Used: Cell Culture, Incubation, Confocal Microscopy, Staining, Fluorescence, Software
Figure Legend Snippet: Characterization of EP1–4 and P2Y2–P2Y4 expression and effect of ionophores on Ca 2+ -mobilization in MEF cells. The expression levels of the prostaglandin receptors EP1–4 and the levels of P2Y2 and P2Y4 were determined by qPCR (a-b). The response to 1 μ M ionomycin (c) and 500 nM thapsigargin (d) on Ca 2+ -mobilization was determined in MEFs overexpressing COX-2, using the dual excitation 340/380 nm protocol as described in Section 2 . MEFs KI were washed with fresh medium to remove PGE 2 accumulated and maintained in the absence or presence of 1 μ M DFU and 5 μ M PGE 2 . Different extracellular concentrations of calcium were used. Results show the mean + SD of three experiments (a-b) or a representative trace (c-d). * P
Techniques Used: Expressing, Real-time Polymerase Chain Reaction
2) Product Images from "Evidence for the Expression of Multiple Uracil Nucleotide-Stimulated P2 Receptors Coupled to Smooth Muscle Contraction in Porcine Isolated Arteries"
Article Title: Evidence for the Expression of Multiple Uracil Nucleotide-Stimulated P2 Receptors Coupled to Smooth Muscle Contraction in Porcine Isolated Arteries
Figure Legend Snippet: RT-PCR and sequence analysis of porcine P2Y2 , P2Y4 and P2Y6 receptors
Techniques Used: Reverse Transcription Polymerase Chain Reaction, Sequencing