p2y 2 r  (Alomone Labs)


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    Structured Review

    Alomone Labs p2y 2 r
    (A) Relative protein expression levels of P2R subtypes <t>(P2Y</t> 1 R, P2Y 2 R, P2Y 4 R, P2X3R, P2X4R and P2X7R) and Panx1 in 8-week-old Akita and age-matched wildtype bone tissue (Data are presented as the means ± SEM *P <0.05 vs. wildtype; n = 6). (B) Representative Western blots for P2Y 2 R, P2Y 4 R, P2X7R and Panx1.
    P2y 2 R, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p2y 2 r/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    p2y 2 r - by Bioz Stars, 2023-02
    94/100 stars

    Images

    1) Product Images from "P2X7R-Panx1 Complex Impairs Bone Mechanosignaling under High Glucose Levels Associated with Type-1 Diabetes"

    Article Title: P2X7R-Panx1 Complex Impairs Bone Mechanosignaling under High Glucose Levels Associated with Type-1 Diabetes

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0155107

    (A) Relative protein expression levels of P2R subtypes (P2Y 1 R, P2Y 2 R, P2Y 4 R, P2X3R, P2X4R and P2X7R) and Panx1 in 8-week-old Akita and age-matched wildtype bone tissue (Data are presented as the means ± SEM *P <0.05 vs. wildtype; n = 6). (B) Representative Western blots for P2Y 2 R, P2Y 4 R, P2X7R and Panx1.
    Figure Legend Snippet: (A) Relative protein expression levels of P2R subtypes (P2Y 1 R, P2Y 2 R, P2Y 4 R, P2X3R, P2X4R and P2X7R) and Panx1 in 8-week-old Akita and age-matched wildtype bone tissue (Data are presented as the means ± SEM *P <0.05 vs. wildtype; n = 6). (B) Representative Western blots for P2Y 2 R, P2Y 4 R, P2X7R and Panx1.

    Techniques Used: Expressing, Western Blot

    Relative protein expression levels of P2R subtypes (P2Y 1 R, P2Y 2 R, P2Y 4 R, P2X7R and P2X3R) and Panx1 in MLO-Y4 (A) and MOB-C (B) cultured in control low glucose (LG), high glucose (HG) and mannitol media (Data are presented as the means ± SEM; ***P <0.001 vs. LG control; n = 5). (C) Representative Western blots of differences in Panx1 and P2Rs observed in osteocytes (A) and osteoblasts (B) .
    Figure Legend Snippet: Relative protein expression levels of P2R subtypes (P2Y 1 R, P2Y 2 R, P2Y 4 R, P2X7R and P2X3R) and Panx1 in MLO-Y4 (A) and MOB-C (B) cultured in control low glucose (LG), high glucose (HG) and mannitol media (Data are presented as the means ± SEM; ***P <0.001 vs. LG control; n = 5). (C) Representative Western blots of differences in Panx1 and P2Rs observed in osteocytes (A) and osteoblasts (B) .

    Techniques Used: Expressing, Cell Culture, Western Blot

    Exposure to high glucose alters the amplitudes of Ca 2+ responses induced in (A) MLO-Y4 by P2X7R stimulation and in (B) MOB-C by P2Y 2 R/P2Y 4 R stimulation. Dose-response curves obtained for the P2X7R agonist BzATP (0.1–1000μM) in MLO-Y4 cells grown in low glucose (LG) and in high glucose (HG) (A) and for the P2Y2/P2Y4R agonist UTP (1–100μM) in MOB-C cells grown in LG and HG (B) conditions. Each point in the dose-response curve corresponds to average amplitude of Ca 2+ responses normalized to control LG maximal amplitude. Data are presented as the means ± SEM, n = 6 independent experiments per condition. Total number of cells analyzed: (A) 431 under HG and 457 under LG; (B) 549 under HG and 620 under LG. Student t-test: *** P<0 . 001 and **** P<0 . 0001 HG vs. LG for each point of the dose-response curve.
    Figure Legend Snippet: Exposure to high glucose alters the amplitudes of Ca 2+ responses induced in (A) MLO-Y4 by P2X7R stimulation and in (B) MOB-C by P2Y 2 R/P2Y 4 R stimulation. Dose-response curves obtained for the P2X7R agonist BzATP (0.1–1000μM) in MLO-Y4 cells grown in low glucose (LG) and in high glucose (HG) (A) and for the P2Y2/P2Y4R agonist UTP (1–100μM) in MOB-C cells grown in LG and HG (B) conditions. Each point in the dose-response curve corresponds to average amplitude of Ca 2+ responses normalized to control LG maximal amplitude. Data are presented as the means ± SEM, n = 6 independent experiments per condition. Total number of cells analyzed: (A) 431 under HG and 457 under LG; (B) 549 under HG and 620 under LG. Student t-test: *** P<0 . 001 and **** P<0 . 0001 HG vs. LG for each point of the dose-response curve.

    Techniques Used:

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    Alomone Labs p2y 2 r
    (A) Relative protein expression levels of P2R subtypes <t>(P2Y</t> 1 R, P2Y 2 R, P2Y 4 R, P2X3R, P2X4R and P2X7R) and Panx1 in 8-week-old Akita and age-matched wildtype bone tissue (Data are presented as the means ± SEM *P <0.05 vs. wildtype; n = 6). (B) Representative Western blots for P2Y 2 R, P2Y 4 R, P2X7R and Panx1.
    P2y 2 R, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p2y 2 r/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    p2y 2 r - by Bioz Stars, 2023-02
    94/100 stars
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    (A) Relative protein expression levels of P2R subtypes (P2Y 1 R, P2Y 2 R, P2Y 4 R, P2X3R, P2X4R and P2X7R) and Panx1 in 8-week-old Akita and age-matched wildtype bone tissue (Data are presented as the means ± SEM *P <0.05 vs. wildtype; n = 6). (B) Representative Western blots for P2Y 2 R, P2Y 4 R, P2X7R and Panx1.

    Journal: PLoS ONE

    Article Title: P2X7R-Panx1 Complex Impairs Bone Mechanosignaling under High Glucose Levels Associated with Type-1 Diabetes

    doi: 10.1371/journal.pone.0155107

    Figure Lengend Snippet: (A) Relative protein expression levels of P2R subtypes (P2Y 1 R, P2Y 2 R, P2Y 4 R, P2X3R, P2X4R and P2X7R) and Panx1 in 8-week-old Akita and age-matched wildtype bone tissue (Data are presented as the means ± SEM *P <0.05 vs. wildtype; n = 6). (B) Representative Western blots for P2Y 2 R, P2Y 4 R, P2X7R and Panx1.

    Article Snippet: The membranes were probed with primary polyclonal antibodies to P2Y 1 R (1:1000; APR-009), P2Y 2 R (1:500; APR-010), P2Y 4 R (1:500; APR-006), P2X1R (1:1000; APR-001), P2X3R (1:1000; APR-016), P2X4R (1:1000; APR-002), P2X7R (1:1000; APR-004, Alomone Labs, Israel), Panx1 (N [Term], 1:100; Cat 487900, Invitrogen), GRP78 BiP (1:5000; ab21685, Abcam, Cambridge, MA, USA) and β-actin (1:35000; A1978, Sigma-Aldrich) followed by incubation with respective horseradish peroxidase (HRP)-conjugated anti-rabbit IgG and anti-mouse IgG (1:10000; Santa Cruz Biotechnology, TX, USA).

    Techniques: Expressing, Western Blot

    Relative protein expression levels of P2R subtypes (P2Y 1 R, P2Y 2 R, P2Y 4 R, P2X7R and P2X3R) and Panx1 in MLO-Y4 (A) and MOB-C (B) cultured in control low glucose (LG), high glucose (HG) and mannitol media (Data are presented as the means ± SEM; ***P <0.001 vs. LG control; n = 5). (C) Representative Western blots of differences in Panx1 and P2Rs observed in osteocytes (A) and osteoblasts (B) .

    Journal: PLoS ONE

    Article Title: P2X7R-Panx1 Complex Impairs Bone Mechanosignaling under High Glucose Levels Associated with Type-1 Diabetes

    doi: 10.1371/journal.pone.0155107

    Figure Lengend Snippet: Relative protein expression levels of P2R subtypes (P2Y 1 R, P2Y 2 R, P2Y 4 R, P2X7R and P2X3R) and Panx1 in MLO-Y4 (A) and MOB-C (B) cultured in control low glucose (LG), high glucose (HG) and mannitol media (Data are presented as the means ± SEM; ***P <0.001 vs. LG control; n = 5). (C) Representative Western blots of differences in Panx1 and P2Rs observed in osteocytes (A) and osteoblasts (B) .

    Article Snippet: The membranes were probed with primary polyclonal antibodies to P2Y 1 R (1:1000; APR-009), P2Y 2 R (1:500; APR-010), P2Y 4 R (1:500; APR-006), P2X1R (1:1000; APR-001), P2X3R (1:1000; APR-016), P2X4R (1:1000; APR-002), P2X7R (1:1000; APR-004, Alomone Labs, Israel), Panx1 (N [Term], 1:100; Cat 487900, Invitrogen), GRP78 BiP (1:5000; ab21685, Abcam, Cambridge, MA, USA) and β-actin (1:35000; A1978, Sigma-Aldrich) followed by incubation with respective horseradish peroxidase (HRP)-conjugated anti-rabbit IgG and anti-mouse IgG (1:10000; Santa Cruz Biotechnology, TX, USA).

    Techniques: Expressing, Cell Culture, Western Blot

    Exposure to high glucose alters the amplitudes of Ca 2+ responses induced in (A) MLO-Y4 by P2X7R stimulation and in (B) MOB-C by P2Y 2 R/P2Y 4 R stimulation. Dose-response curves obtained for the P2X7R agonist BzATP (0.1–1000μM) in MLO-Y4 cells grown in low glucose (LG) and in high glucose (HG) (A) and for the P2Y2/P2Y4R agonist UTP (1–100μM) in MOB-C cells grown in LG and HG (B) conditions. Each point in the dose-response curve corresponds to average amplitude of Ca 2+ responses normalized to control LG maximal amplitude. Data are presented as the means ± SEM, n = 6 independent experiments per condition. Total number of cells analyzed: (A) 431 under HG and 457 under LG; (B) 549 under HG and 620 under LG. Student t-test: *** P<0 . 001 and **** P<0 . 0001 HG vs. LG for each point of the dose-response curve.

    Journal: PLoS ONE

    Article Title: P2X7R-Panx1 Complex Impairs Bone Mechanosignaling under High Glucose Levels Associated with Type-1 Diabetes

    doi: 10.1371/journal.pone.0155107

    Figure Lengend Snippet: Exposure to high glucose alters the amplitudes of Ca 2+ responses induced in (A) MLO-Y4 by P2X7R stimulation and in (B) MOB-C by P2Y 2 R/P2Y 4 R stimulation. Dose-response curves obtained for the P2X7R agonist BzATP (0.1–1000μM) in MLO-Y4 cells grown in low glucose (LG) and in high glucose (HG) (A) and for the P2Y2/P2Y4R agonist UTP (1–100μM) in MOB-C cells grown in LG and HG (B) conditions. Each point in the dose-response curve corresponds to average amplitude of Ca 2+ responses normalized to control LG maximal amplitude. Data are presented as the means ± SEM, n = 6 independent experiments per condition. Total number of cells analyzed: (A) 431 under HG and 457 under LG; (B) 549 under HG and 620 under LG. Student t-test: *** P<0 . 001 and **** P<0 . 0001 HG vs. LG for each point of the dose-response curve.

    Article Snippet: The membranes were probed with primary polyclonal antibodies to P2Y 1 R (1:1000; APR-009), P2Y 2 R (1:500; APR-010), P2Y 4 R (1:500; APR-006), P2X1R (1:1000; APR-001), P2X3R (1:1000; APR-016), P2X4R (1:1000; APR-002), P2X7R (1:1000; APR-004, Alomone Labs, Israel), Panx1 (N [Term], 1:100; Cat 487900, Invitrogen), GRP78 BiP (1:5000; ab21685, Abcam, Cambridge, MA, USA) and β-actin (1:35000; A1978, Sigma-Aldrich) followed by incubation with respective horseradish peroxidase (HRP)-conjugated anti-rabbit IgG and anti-mouse IgG (1:10000; Santa Cruz Biotechnology, TX, USA).

    Techniques: