anti p2x7r (Alomone Labs)


Structured Review

Anti P2x7r, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti p2x7r/product/Alomone Labs
Average 96 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "A Co-Culture System with an Organotypic Lung Slice and an Immortal Alveolar Macrophage Cell Line to Quantify Silica-Induced Inflammation"
Article Title: A Co-Culture System with an Organotypic Lung Slice and an Immortal Alveolar Macrophage Cell Line to Quantify Silica-Induced Inflammation
Journal: PLoS ONE
doi: 10.1371/journal.pone.0117056

Figure Legend Snippet: Primary antibodies used for Western Blot (WB) and immunohistochemistry (IHC).
Techniques Used: Western Blot, Immunohistochemistry
p2x7r staining (Alomone Labs)


Structured Review

P2x7r Staining, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/p2x7r staining/product/Alomone Labs
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Dual Role of the P2X7 Receptor in Dendritic Outgrowth during Physiological and Pathological Brain Development"
Article Title: Dual Role of the P2X7 Receptor in Dendritic Outgrowth during Physiological and Pathological Brain Development
Journal: The Journal of Neuroscience
doi: 10.1523/JNEUROSCI.0805-22.2022

Figure Legend Snippet: P2X7R is present in neurons cultivated in vitro during development. A , Scheme of the experiment. B , Scheme of the regions amplified by qPCR. C , Analysis of the expression of extracellular and ( D ) intracellular sequences of P2X7R by real-time qPCR (DIV1) on each DIV. Relative expression is presented as the fold change normalized to GAPDH expression. E , WT cells were cultured for the indicated period of time, and P2X7R protein expression was determined by immunoblotting. Graphs represent densitometric evaluation of the target protein band normalized to the total amount of loaded protein ( n = 4). F , To validate the specificity of the P2X7R antibody, WT and P2X7R-KO cells were cultured for the indicated period of time, and P2X7R protein expression was determined by immunoblotting. Graphs represent the densitometric evaluation of the target protein band normalized to total amount of loaded protein ( n = 4). G , Areas under the curve for WT and KO primary pyramidal cells at DIV4 after acute BzATP (1 m m ) application. H , Areas under the curve for WT and KO primary pyramidal cells at DIV4 after acute calcium ionophore (1 m m ) application. I , Immunostaining of a neuronal marker (MAP2) and P2X7R in KO and WT primary neurons at DIV10. Nuclei were counterstained with Hoechst. Scale bar, 100 µm. J , Immunostaining for a transfection marker (eGFP) and P2X7R at DIV10. Nuclei were counterstained with Hoechst. Scale bar, 200 µm. * p < 0.05. ** p < 0.005. **** p < 0.0001. ns, non-significant. Data presented as Mean±SEM.
Techniques Used: In Vitro, Amplification, Expressing, Cell Culture, Western Blot, Immunostaining, Marker, Transfection

Figure Legend Snippet: Neurons from P2rx7 −/− mice present less dendritic outgrowth. A , Scheme of the in vitro experiment. B , Immunostaining of a neuronal marker (MAP2) and an astroglial marker (GFAP) and of a transfection marker (eGFP) and a neuronal marker (MAP2) as the control for the transfection in a representative control WT primary hippocampal neuron at DIV10. Nuclei were counterstained with Hoechst. Scale bar, 200 µm. C , Representative hippocampal neurons from WT and P2X7R KO mice transfected with and stained for eGFP. D , Sholl analysis showing the number of intersections versus distance from the soma (radius, μm). E , Using HPLC, nucleotide concentrations in the supernatants of WT and KO primary hippocampal neurons were measured. Quantification of the ( F ) cell body area, ( G ) number of primary dendrites, ( H ) number of endings, and ( I ) mean dendritic length. ** p < 0.005. **** p < 0.0001. ns, non-significant. Data presented as Mean±SEM.
Techniques Used: In Vitro, Immunostaining, Marker, Transfection, Staining

Figure Legend Snippet: Fine-tuning effect of P2X7R on dendritic outgrowth. Antagonists disrupt normal dendritic outgrowth in vitro . A , Scheme of the in vitro experiment. B , Sholl analysis showing the number of intersections versus distance from the soma. Quantification of the ( C ) cell body area, ( D ) number of primary dendrites, ( E ) number of endings, and ( F ) mean dendritic length. Neurons acutely treated with ARL 67156 (100 U/ml) present elongation of dendritic endings. G , Scheme of the in vitro experiment. H , Sholl analysis showing the number of intersections versus distance from the soma. I , Using HPLC, nucleotide concentrations in the supernatants of WT and ARL 67156-treated WT primary hippocampal neurons were measured. Quantification of the ( J ) cell body area, ( K ) number of primary dendrites, ( L ) number of endings, and ( M ) mean total dendritic length. * p < 0.05. ** p < 0.005. *** p < 0.0005. **** p < 0.0001. ns, non-significant. Data presented as Mean±SEM.
Techniques Used: In Vitro

Figure Legend Snippet: Pyramidal neurons from P2rx7 −/− mice present less dendritic outgrowth in the CA1 region of the hippocampus. A , Representative pyramidal hippocampal neurons in slices of the hippocampal CA1 region from P20-29 WT and P2X7R KO mice. B , Sholl analysis showing the number of intersections versus distance from the soma for WT and KO pyramidal neurons. Quantification of the ( C ) cell body area, ( D ) number of primary dendrites, ( E ) number of endings, and ( F ) mean dendritic length. G , Representative pyramidal hippocampal neurons in slices of the hippocampal CA3 region from P20-29 WT and KO mice. H , Sholl analysis showing the number of intersections versus distance from the soma for WT and KO pyramidal neurons. Data are mean ± SEM and analyzed using two-way ANOVA followed by Bonferroni's multiple comparison test. Quantification of the ( I ) cell body area, ( J ) number of primary dendrites, ( K ) number of endings, and ( L ) mean dendritic length. Morphologic deficits correlated with observed deficits in cognitive performance in younger animals. Different aspects of episodic memory, such as ( M ) OL and ( N ) TOR, were analyzed. Deficits in cognitive performance were observed according to both ( O ) the OL index (%) and ( P ) the TOR index (%). Q , Scheme of the experiment. NOR was analyzed ( R ) 2 min after the exploration phase and ( S ) after a longer delay of 24 h. ** p < 0.005. **** p < 0.0001. * p < 0.05. ns, non-significant. Data presented as Mean±SEM.
Techniques Used:

Figure Legend Snippet: P2X7R-deficient mice present weaker synaptic strength. A , Representative traces from the WT and KO groups in the presence of gabazine. B , AMPA/NMDA ratio. C , AMPA-mediated current amplitude. D , NMDA-mediated current amplitude. E , AMPA-mediated current rise time. F , AMPA-mediated current decay time. G , NMDA-mediated current rise time. H , NMDA-mediated current decay time. I , Number of spines on WT and KO neurons. J , Representative segments of WT and KO neurons used to count spines. Scale bar, 50 µm. ** p < 0.005. *** p < 0.0005. ns, non-significant. Data presented as Mean±SEM.
Techniques Used:

Figure Legend Snippet: P2X7R plays a role in a neurodevelopmental model of schizophrenia. A , B , Sholl analysis showing the number of intersections versus distance from the soma for WT neurons, WT neurons treated with different doses of saline or PIC on E12.5, and KO neurons. Quantification of the ( C ) cell body area, ( D ) number of primary dendrites, ( E ) number of endings, and ( F ) mean dendritic length for WT neurons after different treatments. Quantification of the ( G ) cell body area, ( H ) number of primary dendrites, ( I ) number of endings, and ( J ) mean dendritic length for KO neurons after different treatments. K , Using HPLC, nucleotide concentrations in the supernatants of WT and PIC-treated WT primary hippocampal neurons were measured. L , Using HPLC, nucleotide concentrations in the supernatants of KO- and PIC-treated KO primary hippocampal neurons were measured. *** p < 0.0005. **** p < 0.0001. Data presented as Mean±SEM.
Techniques Used:

Figure Legend Snippet: P2X7R drives PIC-induced schizophrenic-like behavior in mice. A , Overview of the experimental protocol. B , Maternal PIC induced elevation in the level of IL-1β in fetal brain samples of WT mice, while this effect was not observed in samples from KO. Values measured are expressed in picograms per mg of total protein. C , Total distance moved (cm) and ( D ) velocity (cm/s) in the open field were not different between WT animals and PIC-treated WT animals. Quantification of ( E ) the spontaneous alternation percentage and ( F ) NOR percentage in the T-maze test showing that MIA-exposed WT animals exhibited cognitive deficits. G , The amounts of time spent exploring the different cages (seconds) in the social preference test were compared between animals of different genotypes and animal subjected to different treatments. H , The startle response was not different between WT and PIC-treated WT animals. I , PPI was disrupted in PIC-treated WT animals. * p < 0.05. ** p < 0.005. *** p < 0.0005. Data presented as Mean±SEM.
Techniques Used:

Figure Legend Snippet: Statistical analysis for molecular and morphologic analysis
Techniques Used: Western Blot, Imaging, Enzyme-linked Immunosorbent Assay
anti p2x7r (Alomone Labs)


Structured Review

Anti P2x7r, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti p2x7r/product/Alomone Labs
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Dual Role of the P2X7 Receptor in Dendritic Outgrowth during Physiological and Pathological Brain Development"
Article Title: Dual Role of the P2X7 Receptor in Dendritic Outgrowth during Physiological and Pathological Brain Development
Journal: The Journal of Neuroscience
doi: 10.1523/JNEUROSCI.0805-22.2022

Figure Legend Snippet: P2X7R is present in neurons cultivated in vitro during development. A , Scheme of the experiment. B , Scheme of the regions amplified by qPCR. C , Analysis of the expression of extracellular and ( D ) intracellular sequences of P2X7R by real-time qPCR (DIV1) on each DIV. Relative expression is presented as the fold change normalized to GAPDH expression. E , WT cells were cultured for the indicated period of time, and P2X7R protein expression was determined by immunoblotting. Graphs represent densitometric evaluation of the target protein band normalized to the total amount of loaded protein ( n = 4). F , To validate the specificity of the P2X7R antibody, WT and P2X7R-KO cells were cultured for the indicated period of time, and P2X7R protein expression was determined by immunoblotting. Graphs represent the densitometric evaluation of the target protein band normalized to total amount of loaded protein ( n = 4). G , Areas under the curve for WT and KO primary pyramidal cells at DIV4 after acute BzATP (1 m m ) application. H , Areas under the curve for WT and KO primary pyramidal cells at DIV4 after acute calcium ionophore (1 m m ) application. I , Immunostaining of a neuronal marker (MAP2) and P2X7R in KO and WT primary neurons at DIV10. Nuclei were counterstained with Hoechst. Scale bar, 100 µm. J , Immunostaining for a transfection marker (eGFP) and P2X7R at DIV10. Nuclei were counterstained with Hoechst. Scale bar, 200 µm. * p < 0.05. ** p < 0.005. **** p < 0.0001. ns, non-significant. Data presented as Mean±SEM.
Techniques Used: In Vitro, Amplification, Expressing, Cell Culture, Western Blot, Immunostaining, Marker, Transfection

Figure Legend Snippet: Neurons from P2rx7 −/− mice present less dendritic outgrowth. A , Scheme of the in vitro experiment. B , Immunostaining of a neuronal marker (MAP2) and an astroglial marker (GFAP) and of a transfection marker (eGFP) and a neuronal marker (MAP2) as the control for the transfection in a representative control WT primary hippocampal neuron at DIV10. Nuclei were counterstained with Hoechst. Scale bar, 200 µm. C , Representative hippocampal neurons from WT and P2X7R KO mice transfected with and stained for eGFP. D , Sholl analysis showing the number of intersections versus distance from the soma (radius, μm). E , Using HPLC, nucleotide concentrations in the supernatants of WT and KO primary hippocampal neurons were measured. Quantification of the ( F ) cell body area, ( G ) number of primary dendrites, ( H ) number of endings, and ( I ) mean dendritic length. ** p < 0.005. **** p < 0.0001. ns, non-significant. Data presented as Mean±SEM.
Techniques Used: In Vitro, Immunostaining, Marker, Transfection, Staining

Figure Legend Snippet: Fine-tuning effect of P2X7R on dendritic outgrowth. Antagonists disrupt normal dendritic outgrowth in vitro . A , Scheme of the in vitro experiment. B , Sholl analysis showing the number of intersections versus distance from the soma. Quantification of the ( C ) cell body area, ( D ) number of primary dendrites, ( E ) number of endings, and ( F ) mean dendritic length. Neurons acutely treated with ARL 67156 (100 U/ml) present elongation of dendritic endings. G , Scheme of the in vitro experiment. H , Sholl analysis showing the number of intersections versus distance from the soma. I , Using HPLC, nucleotide concentrations in the supernatants of WT and ARL 67156-treated WT primary hippocampal neurons were measured. Quantification of the ( J ) cell body area, ( K ) number of primary dendrites, ( L ) number of endings, and ( M ) mean total dendritic length. * p < 0.05. ** p < 0.005. *** p < 0.0005. **** p < 0.0001. ns, non-significant. Data presented as Mean±SEM.
Techniques Used: In Vitro

Figure Legend Snippet: Pyramidal neurons from P2rx7 −/− mice present less dendritic outgrowth in the CA1 region of the hippocampus. A , Representative pyramidal hippocampal neurons in slices of the hippocampal CA1 region from P20-29 WT and P2X7R KO mice. B , Sholl analysis showing the number of intersections versus distance from the soma for WT and KO pyramidal neurons. Quantification of the ( C ) cell body area, ( D ) number of primary dendrites, ( E ) number of endings, and ( F ) mean dendritic length. G , Representative pyramidal hippocampal neurons in slices of the hippocampal CA3 region from P20-29 WT and KO mice. H , Sholl analysis showing the number of intersections versus distance from the soma for WT and KO pyramidal neurons. Data are mean ± SEM and analyzed using two-way ANOVA followed by Bonferroni's multiple comparison test. Quantification of the ( I ) cell body area, ( J ) number of primary dendrites, ( K ) number of endings, and ( L ) mean dendritic length. Morphologic deficits correlated with observed deficits in cognitive performance in younger animals. Different aspects of episodic memory, such as ( M ) OL and ( N ) TOR, were analyzed. Deficits in cognitive performance were observed according to both ( O ) the OL index (%) and ( P ) the TOR index (%). Q , Scheme of the experiment. NOR was analyzed ( R ) 2 min after the exploration phase and ( S ) after a longer delay of 24 h. ** p < 0.005. **** p < 0.0001. * p < 0.05. ns, non-significant. Data presented as Mean±SEM.
Techniques Used:

Figure Legend Snippet: P2X7R-deficient mice present weaker synaptic strength. A , Representative traces from the WT and KO groups in the presence of gabazine. B , AMPA/NMDA ratio. C , AMPA-mediated current amplitude. D , NMDA-mediated current amplitude. E , AMPA-mediated current rise time. F , AMPA-mediated current decay time. G , NMDA-mediated current rise time. H , NMDA-mediated current decay time. I , Number of spines on WT and KO neurons. J , Representative segments of WT and KO neurons used to count spines. Scale bar, 50 µm. ** p < 0.005. *** p < 0.0005. ns, non-significant. Data presented as Mean±SEM.
Techniques Used:

Figure Legend Snippet: P2X7R plays a role in a neurodevelopmental model of schizophrenia. A , B , Sholl analysis showing the number of intersections versus distance from the soma for WT neurons, WT neurons treated with different doses of saline or PIC on E12.5, and KO neurons. Quantification of the ( C ) cell body area, ( D ) number of primary dendrites, ( E ) number of endings, and ( F ) mean dendritic length for WT neurons after different treatments. Quantification of the ( G ) cell body area, ( H ) number of primary dendrites, ( I ) number of endings, and ( J ) mean dendritic length for KO neurons after different treatments. K , Using HPLC, nucleotide concentrations in the supernatants of WT and PIC-treated WT primary hippocampal neurons were measured. L , Using HPLC, nucleotide concentrations in the supernatants of KO- and PIC-treated KO primary hippocampal neurons were measured. *** p < 0.0005. **** p < 0.0001. Data presented as Mean±SEM.
Techniques Used:

Figure Legend Snippet: P2X7R drives PIC-induced schizophrenic-like behavior in mice. A , Overview of the experimental protocol. B , Maternal PIC induced elevation in the level of IL-1β in fetal brain samples of WT mice, while this effect was not observed in samples from KO. Values measured are expressed in picograms per mg of total protein. C , Total distance moved (cm) and ( D ) velocity (cm/s) in the open field were not different between WT animals and PIC-treated WT animals. Quantification of ( E ) the spontaneous alternation percentage and ( F ) NOR percentage in the T-maze test showing that MIA-exposed WT animals exhibited cognitive deficits. G , The amounts of time spent exploring the different cages (seconds) in the social preference test were compared between animals of different genotypes and animal subjected to different treatments. H , The startle response was not different between WT and PIC-treated WT animals. I , PPI was disrupted in PIC-treated WT animals. * p < 0.05. ** p < 0.005. *** p < 0.0005. Data presented as Mean±SEM.
Techniques Used:

Figure Legend Snippet: Statistical analysis for molecular and morphologic analysis
Techniques Used: Western Blot, Imaging, Enzyme-linked Immunosorbent Assay
anti p2x7r (Alomone Labs)


Structured Review

Anti P2x7r, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti p2x7r/product/Alomone Labs
Average 96 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "A Co-Culture System with an Organotypic Lung Slice and an Immortal Alveolar Macrophage Cell Line to Quantify Silica-Induced Inflammation"
Article Title: A Co-Culture System with an Organotypic Lung Slice and an Immortal Alveolar Macrophage Cell Line to Quantify Silica-Induced Inflammation
Journal: PLoS ONE
doi: 10.1371/journal.pone.0117056

Figure Legend Snippet: Primary antibodies used for Western Blot (WB) and immunohistochemistry (IHC).
Techniques Used: Western Blot, Immunohistochemistry
p2x7r (Alomone Labs)


Structured Review

P2x7r, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/p2x7r/product/Alomone Labs
Average 96 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Inhibition of P2X7 Purinergic Receptor Ameliorates Cardiac Fibrosis by Suppressing NLRP3/IL-1 β Pathway"
Article Title: Inhibition of P2X7 Purinergic Receptor Ameliorates Cardiac Fibrosis by Suppressing NLRP3/IL-1 β Pathway
Journal: Oxidative Medicine and Cellular Longevity
doi: 10.1155/2020/7956274

Figure Legend Snippet: Sequences of siRNA to P2X7R.
Techniques Used:

Figure Legend Snippet: Rat RT-qPCR primer sequences.
Techniques Used: Sequencing

Figure Legend Snippet: Mice RT-qPCR primer sequences.
Techniques Used: Sequencing

Figure Legend Snippet: P2X7R was upregulated in the mice heart after TAC surgery. (a) Cardiac images and HW/BW ( n = 8). (b) HE-, Sirius Red-, and Masson's trichrome-stained sections of the hearts following four weeks of TAC treatment ( n = 6). (c) Representative images of an echocardiographic assessment of mice after TAC (4 weeks). Cardiac function indicators measured by echocardiography (LVEF (%), FS (%), LVPWd, IVSd, and LVIDd) in TAC- and sham-operated mice. (d) Expression of CTGF, periostin, and α -SMA mRNAs and proteins in heart cells ( n = 6). (e) Gene expression of P2X receptors in TAC- and sham-operated mice ( n = 6) at 4 weeks and gene expression of P2X7R in heart cells at 3 days, 1 week, 2 weeks, and 4 weeks after TAC- or sham-operated treatment ( n = 6). (f) Expression of P2X7R protein in mouse heart cells ( n = 6). Results are presented as means ± standard error of the mean. ∗ indicates P < 0.05, ∗∗ indicates P < 0.01, and ∗∗∗ indicates P < 0.001 versus sham.
Techniques Used: Staining, Expressing

Figure Legend Snippet: TGF- β 1-treatment upregulated P2X7R in CFs. (a) Immunofluorescence images showing α -SMA expression in CFs ( α -SMA (green) and nuclei DAPI (blue), n = 300). (b) Rate of cell proliferation (EdU-positive cells (red) and nuclei DAPI (blue), n = 120). (c) Expression of TGF- β , α -SMA, CTGF, and periostin mRNAs ( n = 3). (d) Expression of CTGF, α -SMA, periostin, and TGF- β proteins ( n = 3). (e) Western blot results showing P2X7R expression in CFs ( n = 3). (f) Immunofluorescence images showing P2RX7 expression in CFs (P2X7R (red) and nuclei DAPI (blue), n = 60). Results are presented as means ± standard error of the mean; ∗ indicates P < 0.05, ∗∗ indicates P < 0.01, and ∗∗∗ indicates P < 0.001 versus control.
Techniques Used: Immunofluorescence, Expressing, Western Blot

Figure Legend Snippet: Knockdown of P2X7R alleviates the effects of TGF- β 1 on CF activation. (a) Expression of P2X7R mRNA after siP2X7R transfection ( n = 6). (b) Expression of collagen I, CTGF, α -SMA, and TGF- β mRNAs after transfection of CFs with siP2X7R ( n = 6). (c, d) Expression of periostin, α -SMA, CTGF, P2X7R, NLRP3, IL-1 β , and caspase-1 proteins after transfection of CFs with siP2X7R ( n = 6). (e) Expression of α -SMA in CFs by immunofluorescence staining ( α -SMA (green) and nuclei DAPI (blue), n = 300). (f) Changes in CF proliferation (EdU-positive cells (red) and nuclei DAPI (blue), n = 120). (g) Expression of collagen I, CTGF, α -SMA, and TGF- β mRNAs after treatment of CFs with BBG ( n = 6). (h) Expression of P2X7R protein after BBG treatment ( n = 6). (i) Expression of periostin, α -SMA, CTGF, NLRP3, IL-1 β , and caspase-1 proteins after BBG treatment ( n = 6). (j) Expression of periostin, α -SMA, TGF- β and CTGF proteins in activated CFs after BzATP treatment ( n = 6). Results are presented as means ± standard error of the mean; ∗ indicates P < 0.05, ∗∗ indicates P < 0.01, and ∗∗∗ indicates P < 0.001 versus control or siNC; # indicates P < 0.05, ## indicates P < 0.01, and ### indicates P < 0.001.
Techniques Used: Activation Assay, Expressing, Transfection, Immunofluorescence, Staining

Figure Legend Snippet: A schematic illustration of the mechanism of P2X7R in pressure overload-induced cardiac fibrosis and TGF- β 1-induced CF activation. In TAC-induced injury, pathological stress, such as pressure overload and TGF- β 1, triggers the release of ATP. Elevated ATP activates P2X7 receptors, contributing to P2X7-mediated NLRP3 inflammasome (NLRP3, ASC, and caspase-1) activation. This induces the release of IL-1 β , causing severe inflammation that precipitates cardiac fibrosis and aggravates TGF- β 1-induced CF activation. Moreover, inhibition of P2X7R with BBG inhibitor in TAC mice or CFs primed with TGF- β 1 reduces fibrotic extracellular matrix (ECM) gene expression and cardiac fibrosis.
Techniques Used: Activation Assay, Inhibition, Expressing
p2x7r (Alomone Labs)


Structured Review

P2x7r, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/p2x7r/product/Alomone Labs
Average 96 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Loss of P2X7 Receptor Plasma Membrane Expression and Function in Pathogenic B220 + Double-Negative T Lymphocytes of Autoimmune MRL/ lpr Mice"
Article Title: Loss of P2X7 Receptor Plasma Membrane Expression and Function in Pathogenic B220 + Double-Negative T Lymphocytes of Autoimmune MRL/ lpr Mice
Journal: PLoS ONE
doi: 10.1371/journal.pone.0052161

Figure Legend Snippet: Spleen cells from 3 to 4-mo-old MRL +/+ ( solid line ), MRL/ lpr ( dashed line ) and P2X7-deficient B6 P2X7R−/− ( dotted line ) mice (n = 3 mice/group) were treated for 45 min at 37°C with doses of ATP ranging from 100 to 5000 µM. Spleen cells were then triple-stained with anti-CD90, anti-CD19 and anti-CD62L mAb to assess by flow cytometry: (A) the percentage of CD62L-expressing CD19 – CD90 + T cells and (B) MFI of CD62L on CD19 – CD90 + T cells. Results are expressed as the mean percentage of initial expression ± SE.
Techniques Used: Staining, Flow Cytometry, Expressing

Figure Legend Snippet: (A) The levels of CD39 expression on B220 – (either CD4 + or CD8 + ) (□) and B220 + DN (▪) T-cell subsets as well as on CD19 + B cells (▪) from MRL/ lpr mice (n = 3) were analyzed by flow cytometry. Results shown on histogram are normalized MFI (nMFI) calculated as MFI of positive cells × percentage of positive cells. Asterisks denote statistically significant differences between B220 + DN T cells or B220 – CD8 + T cells and B220 – CD4 + T cells: * p ≤0.05; ** p ≤0.01. (B) P2X7R mRNA expression was analyzed in FACS-purified B220 – (□) and B220 + (▪) CD19 – CD90 + T-cell subsets from individual B6, MRL +/+ and MRL/ lpr mice by real-time RT-PCR. Specific P2X7R cDNA was quantified by standard curves based on known amounts of PCR-amplified P2X7R cDNA. Data are expressed as amount (pg) of P2X7R RNA per cell, and histograms represent the mean ± SE of three independent experiments (n = 8 mice/group). (C) P2X7R protein levels in whole LN cells from 3 to 4-mo-old MRL +/+ , MRL/ lpr and B6 P2X7−/− mice, and FACS-sorted B220 – and B220 + CD19 – CD90 + T-cell subsets from MRL/ lpr mice were analyzed by western blotting using affinity-purified rabbit anti-P2X7R polyclonal antibodies (1∶1000; Alomone Laboratories). A non-specific band (*) is frequently observed with this polyclonal antibody . The blot was stripped and reprobed with anti-actin mAb. (D and E) Flow cytometric analysis of P2X7R expression levels on B220 – and B220 + T-cell subpopulations. Spleen cells from MRL +/+ , MRL/ lpr and B6 P2X7R−/− mice (n = 3 mice/group) were stained with anti-CD90 mAb, anti-B220 mAb, rabbit polyclonal anti-P2X7R antiserum (1∶100) generated by genetic immunization and with either anti-CD4 mAb, anti-CD8 mAb or anti-CD4 plus anti-CD8 mAbs. (D) Overlay histograms showing the expression levels of P2X7R on gated CD4 T cells or CD8 T cells from MRL +/+ mice (open histogram) and B6 P2X7R−/− mice (shaded histogram). (E) Overlay histograms comparing the expression levels of P2X7R on B220 – (–) and B220 + (–) T cells, either CD4 + ( left ) or CD8 + ( middle ), from MRL/ lpr mice. Right , overlay histogram comparing the expression levels of P2X7R on B220 + DN T cells (–) and B220 – CD4 + T cells (–) from MRL/ lpr mice. The results shown are representative of at least four independent experiments.
Techniques Used: Expressing, Flow Cytometry, Purification, Quantitative RT-PCR, Amplification, Western Blot, Affinity Purification, Staining, Generated
affinity purified rabbit anti p2x7r antibodies (Alomone Labs)


Structured Review

Affinity Purified Rabbit Anti P2x7r Antibodies, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/affinity purified rabbit anti p2x7r antibodies/product/Alomone Labs
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Loss of P2X7 Receptor Plasma Membrane Expression and Function in Pathogenic B220 + Double-Negative T Lymphocytes of Autoimmune MRL/ lpr Mice"
Article Title: Loss of P2X7 Receptor Plasma Membrane Expression and Function in Pathogenic B220 + Double-Negative T Lymphocytes of Autoimmune MRL/ lpr Mice
Journal: PLoS ONE
doi: 10.1371/journal.pone.0052161

Figure Legend Snippet: Spleen cells from 3 to 4-mo-old MRL +/+ ( solid line ), MRL/ lpr ( dashed line ) and P2X7-deficient B6 P2X7R−/− ( dotted line ) mice (n = 3 mice/group) were treated for 45 min at 37°C with doses of ATP ranging from 100 to 5000 µM. Spleen cells were then triple-stained with anti-CD90, anti-CD19 and anti-CD62L mAb to assess by flow cytometry: (A) the percentage of CD62L-expressing CD19 – CD90 + T cells and (B) MFI of CD62L on CD19 – CD90 + T cells. Results are expressed as the mean percentage of initial expression ± SE.
Techniques Used: Staining, Flow Cytometry, Expressing

Figure Legend Snippet: (A) The levels of CD39 expression on B220 – (either CD4 + or CD8 + ) (□) and B220 + DN (▪) T-cell subsets as well as on CD19 + B cells (▪) from MRL/ lpr mice (n = 3) were analyzed by flow cytometry. Results shown on histogram are normalized MFI (nMFI) calculated as MFI of positive cells × percentage of positive cells. Asterisks denote statistically significant differences between B220 + DN T cells or B220 – CD8 + T cells and B220 – CD4 + T cells: * p ≤0.05; ** p ≤0.01. (B) P2X7R mRNA expression was analyzed in FACS-purified B220 – (□) and B220 + (▪) CD19 – CD90 + T-cell subsets from individual B6, MRL +/+ and MRL/ lpr mice by real-time RT-PCR. Specific P2X7R cDNA was quantified by standard curves based on known amounts of PCR-amplified P2X7R cDNA. Data are expressed as amount (pg) of P2X7R RNA per cell, and histograms represent the mean ± SE of three independent experiments (n = 8 mice/group). (C) P2X7R protein levels in whole LN cells from 3 to 4-mo-old MRL +/+ , MRL/ lpr and B6 P2X7−/− mice, and FACS-sorted B220 – and B220 + CD19 – CD90 + T-cell subsets from MRL/ lpr mice were analyzed by western blotting using affinity-purified rabbit anti-P2X7R polyclonal antibodies (1∶1000; Alomone Laboratories). A non-specific band (*) is frequently observed with this polyclonal antibody . The blot was stripped and reprobed with anti-actin mAb. (D and E) Flow cytometric analysis of P2X7R expression levels on B220 – and B220 + T-cell subpopulations. Spleen cells from MRL +/+ , MRL/ lpr and B6 P2X7R−/− mice (n = 3 mice/group) were stained with anti-CD90 mAb, anti-B220 mAb, rabbit polyclonal anti-P2X7R antiserum (1∶100) generated by genetic immunization and with either anti-CD4 mAb, anti-CD8 mAb or anti-CD4 plus anti-CD8 mAbs. (D) Overlay histograms showing the expression levels of P2X7R on gated CD4 T cells or CD8 T cells from MRL +/+ mice (open histogram) and B6 P2X7R−/− mice (shaded histogram). (E) Overlay histograms comparing the expression levels of P2X7R on B220 – (–) and B220 + (–) T cells, either CD4 + ( left ) or CD8 + ( middle ), from MRL/ lpr mice. Right , overlay histogram comparing the expression levels of P2X7R on B220 + DN T cells (–) and B220 – CD4 + T cells (–) from MRL/ lpr mice. The results shown are representative of at least four independent experiments.
Techniques Used: Expressing, Flow Cytometry, Purification, Quantitative RT-PCR, Amplification, Western Blot, Affinity Purification, Staining, Generated
rabbit anti p2x7r monoclonal igg (Alomone Labs)


Structured Review

Rabbit Anti P2x7r Monoclonal Igg, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti p2x7r monoclonal igg/product/Alomone Labs
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "The Actions and Mechanisms of P2X7R and p38 MAPK Activation in Mediating Bortezomib-Induced Neuropathic Pain"
Article Title: The Actions and Mechanisms of P2X7R and p38 MAPK Activation in Mediating Bortezomib-Induced Neuropathic Pain
Journal: BioMed Research International
doi: 10.1155/2020/8143754

Figure Legend Snippet: The sequences of oligonucleotide primers.
Techniques Used:

Figure Legend Snippet: The antibodies for immunoblotting.
Techniques Used: Western Blot, Concentration Assay

Figure Legend Snippet: The antibodies for fluorescence labeling.
Techniques Used: Fluorescence, Labeling, Concentration Assay

Figure Legend Snippet: Mechanical threshold and P2X7R and p-p38 expression. (a) Mechanical threshold after BTZ injection. (b, c) Western blot for P2X7R expression after BTZ treatment. (d) Immunofluorescence location of P2X7R in DRG. The arrows indicate the typical single- or double-labeled DRG neurons and satellite cells. P2X7R is not expressed in NF-200-positive neurons. P2X7R is expressed in GFAP-labeled satellite glial cells (SGCs). (e) Immunofluorescence location of p-p38 in DRG. The arrows indicate the typical single- or double-labeled DRG neurons and satellite cells. p-p38 is expressed in both MAP2-labeled neurons and GFAP-labeled SGCs. (f) Immunofluorescence location of P2X7R in SDH. The arrows indicate the typical single-labeled and double-labeled cells in SDH. P2X7R is expressed mainly in Iba-1-labeled microglial cells rather than in GFAP-labeled astrocytes and MAP2-labeled neurons. (g) Immunofluorescence location of p-p38 in SDH. The arrows indicate the typical single-labeled and double-labeled cells in SDH. p-p38 is expressed mainly in Iba-1-labeled microglial cells. Scale bar = 50 μ m. Mean ± SEM ( n = 5). ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001.
Techniques Used: Expressing, Injection, Western Blot, Immunofluorescence, Labeling

Figure Legend Snippet: p38 mRNA expression and p38 phosphorylation in DRG after inhibition of P2X7R with BBG. (a) p38 mRNA levels. (b) p-p38 protein immunoblotting bands. (c) p-p38 protein levels. (d) p-p38 immunofluorescence labeling. The arrows show the typical p-p38 single-labeled DRG cells. (e) p-p38 fluorescence density. Scale bar = 50 μ m. Mean ± SEM ( n = 5). ∗ P < 0.05; ∗∗∗ P < 0.001.
Techniques Used: Expressing, Inhibition, Western Blot, Immunofluorescence, Labeling, Fluorescence

Figure Legend Snippet: p38 mRNA expression and p38 phosphorylation in SDH after inhibition of P2X7R with BBG. (a) p38 mRNA levels. (b) p-p38 protein immunoblotting bands. (c) p-p38 protein levels. (d) P2X7R and p-p38 coexpression fluorescence labeling. The arrows indicate the typical single-labeled and double-labeled SDH microglia. (e) P2X7R and p-p38 coexpression fluorescence density. Scale bar = 50 μ m. Mean ± SEM ( n = 5). ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001.
Techniques Used: Expressing, Inhibition, Western Blot, Fluorescence, Labeling

Figure Legend Snippet: IL-1 β , IL-6, and TNF- α mRNA expression in DRG and SDH after inhibition of P2X7R. (a) DRG IL-1 β mRNA. (b) DRG IL-6 mRNA. (c) DRG TNF- α mRNA. (d) SDH IL-1 β mRNA. (e) SDH IL-6 mRNA. (f) SDH TNF- α mRNA. Mean ± SEM ( n = 5). ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001.
Techniques Used: Expressing, Inhibition

Figure Legend Snippet: P2X7R mRNA and protein expression in DRG after inhibition of p38 phosphorylation. (a) P2X7R mRNA levels. (b) P2X7R protein immunoblotting bands. (c) P2X7R protein levels. (d) P2X7R and GFAP coexpression fluorescence labeling for SGCs. The arrows indicate the typical single-labeled and double-labeled DRG satellite cells. (e) P2X7R and GFAP coexpression fluorescence density. Scale bar = 50 μ m. Mean ± SEM ( n = 5). ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001.
Techniques Used: Expressing, Inhibition, Western Blot, Fluorescence, Labeling

Figure Legend Snippet: P2X7R mRNA and protein expression in SDH after inhibition of p38 phosphorylation. (a) P2X7R mRNA levels. (b) P2X7R protein immunoblotting bands. (c) P2X7R protein levels. (d) P2X7R and p-p38 coexpression fluorescence labeling. The arrows indicate the typical single-labeled and double-labeled SDH microglia. (e) P2X7R and p-p38 colocalization fluorescence density. Scale bar = 50 μ m. Mean ± SEM ( n = 5). ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001.
Techniques Used: Expressing, Inhibition, Western Blot, Fluorescence, Labeling

Figure Legend Snippet: Mechanical threshold alterations after inhibition of P2X7R or p38. (a) Mechanical threshold after inhibition of P2X7R. (b) Mechanical threshold after inhibition of p38. Mean ± SEM ( n = 5). ∗∗∗ P < 0.001 (vs. control); # P < 0.05; ## P < 0.01 (vs. BTZ group).
Techniques Used: Inhibition
rabbit anti p2x7r monoclonal igg (Alomone Labs)


Structured Review

Rabbit Anti P2x7r Monoclonal Igg, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti p2x7r monoclonal igg/product/Alomone Labs
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "The Actions and Mechanisms of P2X7R and p38 MAPK Activation in Mediating Bortezomib-Induced Neuropathic Pain"
Article Title: The Actions and Mechanisms of P2X7R and p38 MAPK Activation in Mediating Bortezomib-Induced Neuropathic Pain
Journal: BioMed Research International
doi: 10.1155/2020/8143754

Figure Legend Snippet: The sequences of oligonucleotide primers.
Techniques Used:

Figure Legend Snippet: The antibodies for immunoblotting.
Techniques Used: Western Blot, Concentration Assay

Figure Legend Snippet: The antibodies for fluorescence labeling.
Techniques Used: Fluorescence, Labeling, Concentration Assay

Figure Legend Snippet: Mechanical threshold and P2X7R and p-p38 expression. (a) Mechanical threshold after BTZ injection. (b, c) Western blot for P2X7R expression after BTZ treatment. (d) Immunofluorescence location of P2X7R in DRG. The arrows indicate the typical single- or double-labeled DRG neurons and satellite cells. P2X7R is not expressed in NF-200-positive neurons. P2X7R is expressed in GFAP-labeled satellite glial cells (SGCs). (e) Immunofluorescence location of p-p38 in DRG. The arrows indicate the typical single- or double-labeled DRG neurons and satellite cells. p-p38 is expressed in both MAP2-labeled neurons and GFAP-labeled SGCs. (f) Immunofluorescence location of P2X7R in SDH. The arrows indicate the typical single-labeled and double-labeled cells in SDH. P2X7R is expressed mainly in Iba-1-labeled microglial cells rather than in GFAP-labeled astrocytes and MAP2-labeled neurons. (g) Immunofluorescence location of p-p38 in SDH. The arrows indicate the typical single-labeled and double-labeled cells in SDH. p-p38 is expressed mainly in Iba-1-labeled microglial cells. Scale bar = 50 μ m. Mean ± SEM ( n = 5). ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001.
Techniques Used: Expressing, Injection, Western Blot, Immunofluorescence, Labeling

Figure Legend Snippet: p38 mRNA expression and p38 phosphorylation in DRG after inhibition of P2X7R with BBG. (a) p38 mRNA levels. (b) p-p38 protein immunoblotting bands. (c) p-p38 protein levels. (d) p-p38 immunofluorescence labeling. The arrows show the typical p-p38 single-labeled DRG cells. (e) p-p38 fluorescence density. Scale bar = 50 μ m. Mean ± SEM ( n = 5). ∗ P < 0.05; ∗∗∗ P < 0.001.
Techniques Used: Expressing, Inhibition, Western Blot, Immunofluorescence, Labeling, Fluorescence

Figure Legend Snippet: p38 mRNA expression and p38 phosphorylation in SDH after inhibition of P2X7R with BBG. (a) p38 mRNA levels. (b) p-p38 protein immunoblotting bands. (c) p-p38 protein levels. (d) P2X7R and p-p38 coexpression fluorescence labeling. The arrows indicate the typical single-labeled and double-labeled SDH microglia. (e) P2X7R and p-p38 coexpression fluorescence density. Scale bar = 50 μ m. Mean ± SEM ( n = 5). ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001.
Techniques Used: Expressing, Inhibition, Western Blot, Fluorescence, Labeling

Figure Legend Snippet: IL-1 β , IL-6, and TNF- α mRNA expression in DRG and SDH after inhibition of P2X7R. (a) DRG IL-1 β mRNA. (b) DRG IL-6 mRNA. (c) DRG TNF- α mRNA. (d) SDH IL-1 β mRNA. (e) SDH IL-6 mRNA. (f) SDH TNF- α mRNA. Mean ± SEM ( n = 5). ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001.
Techniques Used: Expressing, Inhibition

Figure Legend Snippet: P2X7R mRNA and protein expression in DRG after inhibition of p38 phosphorylation. (a) P2X7R mRNA levels. (b) P2X7R protein immunoblotting bands. (c) P2X7R protein levels. (d) P2X7R and GFAP coexpression fluorescence labeling for SGCs. The arrows indicate the typical single-labeled and double-labeled DRG satellite cells. (e) P2X7R and GFAP coexpression fluorescence density. Scale bar = 50 μ m. Mean ± SEM ( n = 5). ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001.
Techniques Used: Expressing, Inhibition, Western Blot, Fluorescence, Labeling

Figure Legend Snippet: P2X7R mRNA and protein expression in SDH after inhibition of p38 phosphorylation. (a) P2X7R mRNA levels. (b) P2X7R protein immunoblotting bands. (c) P2X7R protein levels. (d) P2X7R and p-p38 coexpression fluorescence labeling. The arrows indicate the typical single-labeled and double-labeled SDH microglia. (e) P2X7R and p-p38 colocalization fluorescence density. Scale bar = 50 μ m. Mean ± SEM ( n = 5). ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001.
Techniques Used: Expressing, Inhibition, Western Blot, Fluorescence, Labeling

Figure Legend Snippet: Mechanical threshold alterations after inhibition of P2X7R or p38. (a) Mechanical threshold after inhibition of P2X7R. (b) Mechanical threshold after inhibition of p38. Mean ± SEM ( n = 5). ∗∗∗ P < 0.001 (vs. control); # P < 0.05; ## P < 0.01 (vs. BTZ group).
Techniques Used: Inhibition
rabbit p2x7r antibody (Alomone Labs)


Structured Review

Rabbit P2x7r Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit p2x7r antibody/product/Alomone Labs
Average 96 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "P2X7 Receptor (P2X7R) of Microglia Mediates Neuroinflammation by Regulating (NOD)-Like Receptor Protein 3 (NLRP3) Inflammasome-Dependent Inflammation After Spinal Cord Injury"
Article Title: P2X7 Receptor (P2X7R) of Microglia Mediates Neuroinflammation by Regulating (NOD)-Like Receptor Protein 3 (NLRP3) Inflammasome-Dependent Inflammation After Spinal Cord Injury
Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research
doi: 10.12659/MSM.925491

Figure Legend Snippet: Immunofluorescence detected P2X7R of microglia in spinal cord. ( A ) P2X7R (P2X7R+, green) and IBA-1 (IBA-1+, red) co-expressed as P2X7R of microglia (P2X7R+/IBA-1+, yellow) that were overexpressed after spinal cord injury and BzATP intervention, and, compared to the morphology of microglia in the sham group, the morphology of activated microglia after injury changed to large soma and short irregular processes, revealing that many microglia in spinal cord became active after spinal cord injury. ( B ) The number of P2X7R of microglia (P2X7R+/IBA-1+) increased notably after spinal cord injury compared to the sham group (** P<0.01), and, compared to the model group, BzATP increased the number of P2X7R of microglia (* P<0.05) while A-438079 significantly decreased the number of P2X7R of microglia (** P<0.01). ( C ) The P2X7R-positive microglia percentage was clearly increased after spinal cord injury compared to the sham group (** P<0.01), and, compared to the model group, BzATP accounted for a significantly increased P2X7R-positive microglia proportion (** P<0.01) while A-438079 accounted for a significantly reduced P2X7R-positive microglia proportion (** P<0.01).
Techniques Used: Immunofluorescence

Figure Legend Snippet: Western blot and qPCR assay were used to assess the expression of P2X7R protein and P2X7R mRNA in spinal cord. ( A ) Western blot assay showed that the expression of P2X7R protein in spinal cord was increased notably after spinal cord injury compared to the sham group (** P<0.01) and, compared to model group, BzATP increased the expression of P2X7R protein in spinal cord (* P<0.05) while A-438079 significantly decreased the expression of P2X7R protein in spinal cord (** P<0.01). ( B ) qPCR assay showed that the expression of P2X7R mRNA in spinal cord increased notably after spinal cord injury compared to the sham group (** P<0.01), and compared to the model group, BzATP increased the expression of P2X7R mRNA in spinal cord (* P<0.05) while A-438079 significantly decreased the expression of P2X7R mRNA in spinal cord (** P<0.01).
Techniques Used: Western Blot, Expressing

Figure Legend Snippet: P2X7R of microglia in spinal cord-mediated neuroinflammation after spinal cord injury. ( A ) ELISA showed that, compared to the sham group, the expression of IL-1β and IL-18 in the spinal cord were significantly increased after spinal cord injury (** P<0.01) and, compared to the model group, A438079 significantly inhibited the expression of IL- 1β and IL-18 (** P<0.01) and BzATP significantly increased the expression of IL- 1β and IL-18 (** P<0.01), which was strongly inhibited by CY-09 (** P<0.01). ( B ) qPCR assay showed that, compared to the sham group, the expression of IL-1β mRNA and IL-18 mRNA in the spinal cord was significantly increased after spinal cord injury (** P<0.01) and, compared to the model group, A438079 significantly inhibited the expression of IL-1β mRNA and IL-18 mRNA (** P<0.01), and BzATP significantly increased the expression of IL-1β mRNA and IL-18 mRNA (** P<0.01), which was strongly inhibited by CY-09 (** P<0.01).
Techniques Used: Enzyme-linked Immunosorbent Assay, Expressing

Figure Legend Snippet: P2X7R of microglia in spinal cord regulated the expression of NLRP3 and cleaved-Caspase-1 (P20). Compared to the sham group, the expression of NLRP3 and cleaved-Caspase-1 (P20) in the spinal cord significantly increased after spinal cord injury (** P<0.01) and, compared to the model group, BzATP promoted the expression of NLRP3 and cleaved-Caspase-1 (P20) (* P<0.05), which was inhibited by CY-09 (* P<0.05), while A-438079 notably reduced the expression of NLRP3 and cleaved-Caspase-1 (P20) (** P<0.01).
Techniques Used: Expressing
rabbit p2x7r antibody (Alomone Labs)


Structured Review

Rabbit P2x7r Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit p2x7r antibody/product/Alomone Labs
Average 96 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "P2X7 Receptor (P2X7R) of Microglia Mediates Neuroinflammation by Regulating (NOD)-Like Receptor Protein 3 (NLRP3) Inflammasome-Dependent Inflammation After Spinal Cord Injury"
Article Title: P2X7 Receptor (P2X7R) of Microglia Mediates Neuroinflammation by Regulating (NOD)-Like Receptor Protein 3 (NLRP3) Inflammasome-Dependent Inflammation After Spinal Cord Injury
Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research
doi: 10.12659/MSM.925491

Figure Legend Snippet: Immunofluorescence detected P2X7R of microglia in spinal cord. ( A ) P2X7R (P2X7R+, green) and IBA-1 (IBA-1+, red) co-expressed as P2X7R of microglia (P2X7R+/IBA-1+, yellow) that were overexpressed after spinal cord injury and BzATP intervention, and, compared to the morphology of microglia in the sham group, the morphology of activated microglia after injury changed to large soma and short irregular processes, revealing that many microglia in spinal cord became active after spinal cord injury. ( B ) The number of P2X7R of microglia (P2X7R+/IBA-1+) increased notably after spinal cord injury compared to the sham group (** P<0.01), and, compared to the model group, BzATP increased the number of P2X7R of microglia (* P<0.05) while A-438079 significantly decreased the number of P2X7R of microglia (** P<0.01). ( C ) The P2X7R-positive microglia percentage was clearly increased after spinal cord injury compared to the sham group (** P<0.01), and, compared to the model group, BzATP accounted for a significantly increased P2X7R-positive microglia proportion (** P<0.01) while A-438079 accounted for a significantly reduced P2X7R-positive microglia proportion (** P<0.01).
Techniques Used: Immunofluorescence

Figure Legend Snippet: Western blot and qPCR assay were used to assess the expression of P2X7R protein and P2X7R mRNA in spinal cord. ( A ) Western blot assay showed that the expression of P2X7R protein in spinal cord was increased notably after spinal cord injury compared to the sham group (** P<0.01) and, compared to model group, BzATP increased the expression of P2X7R protein in spinal cord (* P<0.05) while A-438079 significantly decreased the expression of P2X7R protein in spinal cord (** P<0.01). ( B ) qPCR assay showed that the expression of P2X7R mRNA in spinal cord increased notably after spinal cord injury compared to the sham group (** P<0.01), and compared to the model group, BzATP increased the expression of P2X7R mRNA in spinal cord (* P<0.05) while A-438079 significantly decreased the expression of P2X7R mRNA in spinal cord (** P<0.01).
Techniques Used: Western Blot, Expressing

Figure Legend Snippet: P2X7R of microglia in spinal cord-mediated neuroinflammation after spinal cord injury. ( A ) ELISA showed that, compared to the sham group, the expression of IL-1β and IL-18 in the spinal cord were significantly increased after spinal cord injury (** P<0.01) and, compared to the model group, A438079 significantly inhibited the expression of IL- 1β and IL-18 (** P<0.01) and BzATP significantly increased the expression of IL- 1β and IL-18 (** P<0.01), which was strongly inhibited by CY-09 (** P<0.01). ( B ) qPCR assay showed that, compared to the sham group, the expression of IL-1β mRNA and IL-18 mRNA in the spinal cord was significantly increased after spinal cord injury (** P<0.01) and, compared to the model group, A438079 significantly inhibited the expression of IL-1β mRNA and IL-18 mRNA (** P<0.01), and BzATP significantly increased the expression of IL-1β mRNA and IL-18 mRNA (** P<0.01), which was strongly inhibited by CY-09 (** P<0.01).
Techniques Used: Enzyme-linked Immunosorbent Assay, Expressing

Figure Legend Snippet: P2X7R of microglia in spinal cord regulated the expression of NLRP3 and cleaved-Caspase-1 (P20). Compared to the sham group, the expression of NLRP3 and cleaved-Caspase-1 (P20) in the spinal cord significantly increased after spinal cord injury (** P<0.01) and, compared to the model group, BzATP promoted the expression of NLRP3 and cleaved-Caspase-1 (P20) (* P<0.05), which was inhibited by CY-09 (* P<0.05), while A-438079 notably reduced the expression of NLRP3 and cleaved-Caspase-1 (P20) (** P<0.01).
Techniques Used: Expressing