Journal: PLoS ONE
Article Title: Age-Related Nuclear Translocation of P2X6 Subunit Modifies Splicing Activity Interacting with Splicing Factor 3A1
Figure Lengend Snippet: Expression of P2X6 subunit in adult mice hippocampus. ( a-c ) Immunohistochemical analysis of hippocampus sections from 8 month-old mice. Cells were labeled with antibodies against P2X6 ( a ), P2X4 ( b ) and P2X2 ( c ) subunits. Insets depict enlarged views (40X magnification) of CA3 hippocampal region showing a dotted pattern for P2X6 into the nucleus in addition to cytoplasmic staining ( a ), somatodendritic stainining for P2X4 ( b ) and axonal and cytoplasmic staining for P2X2( c ). Scale bar 200 μm. ( d-f ) Immuno-electron microscopy analysis of a CA3 hippocampal neuron from 8 months-old wild type mouse labeled with anti-P2X6 antibody. The staining is observed both in the cytoplasm (cyt, white arrows) and into the nucleus (nuc, white asterisks). Scale bar 2 μm. ( d-e ) Inset of the area indicated in ( e ) showing a 3.5 X magnification of this indicated area. P2X6 positive regions are mainly located in patches added to the inner nuclear membrane ( e ).
Article Snippet: Brain sections were pretreated for 1 h with 1% bovine serum albumin (BSA) (Sigma-Aldrich), 5% FBS, and 0.2% Triton X-100 (Sigma-Aldrich) in PBS, and subsequently incubated with rabbit polyclonal antibodies against P2X6 (Alomone Labs, 1/50), P2X4 (Alomone Labs, 1/500), and/or P2X2 (Alomone Labs, 1/200) receptors.
Techniques: Expressing, Mouse Assay, Immunohistochemistry, Labeling, Staining, Immuno-Electron Microscopy