Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Article Title: Molecular mechanisms of voiding dysfunction in a novel mouse model of acute urinary retention

doi: 10.1096/fj.202002415R

Figure Lengend Snippet: Altered receptor expression in mediating diminished BSM contractility upon pressure treatment. A, Western blot images of M2, M3, and P2X1 receptor protein expression in mouse bladders from control (n = 4) and pressure treated mice (50 cm: n = 4 for 0 hours, 6 hours, and 24 hours, respectively. 80 cm, n = 4 for 0 hours, 6 hours, and 24 hours, respectively). B-D, are quantitated data by densitometry and normalized to actin. Data are shown as box and whiskers, center line is the median of the data set, box represents 75% of the data, and bars indicate whiskers from minimum to maximum. Data were analyzed by Student’s t test between control group and 50 or 80 cm H2O pressure treated groups. *P < .05, **P < .001

Article Snippet: Rabbit anti-P2X1 antibody (1:1000, #APR-001) and rabbit anti-chrm2 antibody (1:1000, #AMR-002) were purchased from Alomone Lab. Rabbit anti-chrm3 antibody (1:1000, #PA5–77485) was purchased from Thermo Fisher Scientific.

Techniques: Expressing, Western Blot

Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Article Title: Molecular mechanisms of voiding dysfunction in a novel mouse model of acute urinary retention

doi: 10.1096/fj.202002415R

Figure Lengend Snippet: Altered receptor expression in mediating diminished BSM contractility upon pressure treatment. A, Western blot images of M2, M3, and P2X1 receptor protein expression in mouse bladders from control (n = 4) and pressure treated mice (50 cm: n = 4 for 0 hours, 6 hours, and 24 hours, respectively. 80 cm, n = 4 for 0 hours, 6 hours, and 24 hours, respectively). B-D, are quantitated data by densitometry and normalized to actin. Data are shown as box and whiskers, center line is the median of the data set, box represents 75% of the data, and bars indicate whiskers from minimum to maximum. Data were analyzed by Student’s t test between control group and 50 or 80 cm H2O pressure treated groups. *P < .05, **P < .001

Article Snippet: Western blot analysis Rabbit anti-P2X1 antibody (1:1000, #APR-001) and rabbit anti-chrm2 antibody (1:1000, #AMR-002) were purchased from Alomone Lab. Rabbit anti-chrm3 antibody (1:1000, #PA5–77485) was purchased from Thermo Fisher Scientific.

Techniques: Expressing, Western Blot

Journal: PLoS ONE

Article Title: A Model of Ischemia-Induced Neuroblast Activation in the Adult Subventricular Zone

doi: 10.1371/journal.pone.0005278

Figure Lengend Snippet: List of antibodies used for fluorescence immunochemistry.

Article Snippet: P2X1 , 1/500 , Rabbit , Alomone, Jerusalem-Israel.

Techniques: Fluorescence

Journal: PLoS ONE

Article Title: A Model of Ischemia-Induced Neuroblast Activation in the Adult Subventricular Zone

doi: 10.1371/journal.pone.0005278

Figure Lengend Snippet: The expression of a subset of P2X receptors is modulated after OGD.

Article Snippet: P2X1 , 1/500 , Rabbit , Alomone, Jerusalem-Israel.

Techniques: Expressing

Journal: PLoS ONE

Article Title: Sensory Dysfunction of Bladder Mucosa and Bladder Oversensitivity in a Rat Model of Metabolic Syndrome

doi: 10.1371/journal.pone.0045578

Figure Lengend Snippet: Western blot analysis with specific antibodies to the TRPV1 receptor, purinergic P2X 2 receptor, and P2X 3 receptor of the rat mucosal layer and the purinergic P2X 1 receptor of the rat smooth muscle layer in controls and FFRs with different in cystometric presentations. A. TRPV1 receptor: The TRPV1 antibody produced a clear single band at 95kDa. B. Purinergic P2X 2 receptor C. Purinergic P2X 3 mature receptor: the predominant P2X 3 form (65kDa). D. Up-regulation of P2X3 native and intermediate polypeptides (up to 55kD) were shown in both FFR groups. E. Purinergic P2X 1 receptor Experiments were repeated two times and representative blots are shown. Data of proteins expression (ratios of signal intensities of investigated receptors relative to β-actin or GAPDH) were calculated with 8 samples in each group. These data of Mean ± SE were standardized and expressed in percentage in which the value of the control group is treated as 100%. Theses values were shown in the bar graph. An asterisk indicates a significant difference between controls and FFR groups (One-way ANOVA with Dunnett’s test, p<0.05).

Article Snippet: Antibodies raised against TRPV1 receptor (Alomone, Israel; 1∶1000 dilution), P2X 2 receptor (Alomone, Israel; 1∶500 dilution), P2X 3 receptor (Neuromics, MN; 1∶500 dilution), P2X 1 receptor (Alomone, Israel; 1∶500 dilution), iNOS (Cayman Chemical, Michigan; 1∶500 dilution), eNOS (BD Biosciences, CA; 1∶1000 dilution), GAPDH (Millipore, CA; 1∶10000 dilution ) and β-actin (Millipore, CA; 1∶10000 dilution) were used.

Techniques: Western Blot, Produced, Expressing

Journal: PLoS ONE

Article Title: Sensory Dysfunction of Bladder Mucosa and Bladder Oversensitivity in a Rat Model of Metabolic Syndrome

doi: 10.1371/journal.pone.0045578

Figure Lengend Snippet: Western blot analysis with specific antibodies to the TRPV1 receptor, purinergic P2X 2 receptor, and P2X 3 receptor of the rat mucosal layer and the purinergic P2X 1 receptor of the rat smooth muscle layer in controls and FFRs with different in cystometric presentations. A. TRPV1 receptor: The TRPV1 antibody produced a clear single band at 95kDa. B. Purinergic P2X 2 receptor C. Purinergic P2X 3 mature receptor: the predominant P2X 3 form (65kDa). D. Up-regulation of P2X3 native and intermediate polypeptides (up to 55kD) were shown in both FFR groups. E. Purinergic P2X 1 receptor Experiments were repeated two times and representative blots are shown. Data of proteins expression (ratios of signal intensities of investigated receptors relative to β-actin or GAPDH) were calculated with 8 samples in each group. These data of Mean ± SE were standardized and expressed in percentage in which the value of the control group is treated as 100%. Theses values were shown in the bar graph. An asterisk indicates a significant difference between controls and FFR groups (One-way ANOVA with Dunnett’s test, p<0.05).

Article Snippet: Antibodies raised against TRPV1 receptor (Alomone, Israel; 1∶1000 dilution), P2X 2 receptor (Alomone, Israel; 1∶500 dilution), P2X 3 receptor (Neuromics, MN; 1∶500 dilution), P2X 1 receptor (Alomone, Israel; 1∶500 dilution), iNOS (Cayman Chemical, Michigan; 1∶500 dilution), eNOS (BD Biosciences, CA; 1∶1000 dilution), GAPDH (Millipore, CA; 1∶10000 dilution ) and β-actin (Millipore, CA; 1∶10000 dilution) were used.

Techniques: Western Blot, Produced, Expressing

Journal: BioMed Research International

Article Title: Retrograde Labeling of Different Distribution Features of DRG P2X2 and P2X3 Receptors in a Neuropathic Pain Rat Model

doi: 10.1155/2020/9861459

Figure Lengend Snippet: Expression and size distribution of P2X1-positive cells to total and FG-positive DRG neurons between the control and CCI groups. (a) P2X1-positive fluorescence images of total and FG-positive DRG between the control and CCI groups (200x, scale is 50 μ m). (b) The percentages of the expression and size distribution of P2X1-positive cells to total and FG-positive DRG neurons between the control and CCI groups. There was no significant difference between the control and CCI groups, n = 6.

Article Snippet: The primary antibodies were rabbit anti-P2X1-6 (RRID/Cat: AB_2341048, #APR-022; AB_2341051, #APR-025; AB_2341052, #APR-026; AB_2341050, #APR-024; AB_2756757, #APR-027; and AB_2756758, #APR-028, respectively, diluted 1 : 200 with 0.1 M PBS, Alomone Labs, Israel).

Techniques: Expressing, Fluorescence

Journal: eLife

Article Title: EROS is a selective chaperone regulating the phagocyte NADPH oxidase and purinergic signalling

doi: 10.7554/eLife.76387

Figure Lengend Snippet: ( A–D ) P2X7 expression analysed by Western blotting of macrophages isolated from control, EROS knockout (KO) ( A ) and gp91 phox KO mice ( B ), induced pluripotent stem cells (iPS)-derived macrophages control or EROS-deficient ( C ) and of control PLB985 cells and an EROS-deficient clone ( D ). ( E, F ) P2X7 expression in RAW264.7 cells overexpressing a FLAG-tagged EROS vector ( E ) and in HEK293 cells transiently expressing the specified constructs ( F ). ( G, H ) Interaction between EROS and P2X7 probed by immunoprecipitation (IP) of EROS from RAW264.7 EROS-FLAG macrophages followed by immunoblot (IB) for P2X7 ( G ) and by Nanoluc Binary Technology (NanoBIT) assay in live HEK293 cells expressing the LgBIT-fused EROS vector with a SmBIT-fused P2X7 vector ( H ). ( I ) P2X1 expression in macrophages isolated from EROS KO mice compared to control. n = 5 biological replicates. ( J ) P2X1 abundance upon co-transfection with EROS construct in HEK293 cells. Data are representative of hree independent experiments; error bars indicate SEM of triplicates. See also and . Figure 5—source data 1. Raw unedited blots for . Figure 5—source data 2. Raw unedited blots for . Figure 5—source data 3. Raw unedited blots for . Figure 5—source data 4. Uncropped gels used for .

Article Snippet: Antibody , Anti-P2X1 (rabbit polyclonal) Anti-P2X4 (rabbit polyclonal) Anti-P2X7 (rabbit polyclonal) , Alomone , APR001 APR002 APR004 , (1:250) (1:500) (1:500).

Techniques: Expressing, Western Blot, Isolation, Knock-Out, Derivative Assay, Plasmid Preparation, Construct, Immunoprecipitation, Cotransfection

Journal: eLife

Article Title: EROS is a selective chaperone regulating the phagocyte NADPH oxidase and purinergic signalling

doi: 10.7554/eLife.76387

Figure Lengend Snippet:

Article Snippet: Antibody , Anti-P2X1 (rabbit polyclonal) Anti-P2X4 (rabbit polyclonal) Anti-P2X7 (rabbit polyclonal) , Alomone , APR001 APR002 APR004 , (1:250) (1:500) (1:500).

Techniques: Knock-Out, Generated, Over Expression

Journal: eLife

Article Title: EROS is a selective chaperone regulating the phagocyte NADPH oxidase and purinergic signalling

doi: 10.7554/eLife.76387

Figure Lengend Snippet:

Article Snippet: Antibody , Anti-P2X1 (rabbit polyclonal) Anti-P2X4 (rabbit polyclonal) Anti-P2X7 (rabbit polyclonal) , Alomone , APR001 APR002 APR004 , (1:250) (1:500) (1:500).

Techniques: Knock-Out, Generated, Over Expression