Journal: Journal of visualized experiments : JoVE

Article Title: A Spin-Tip Enrichment Strategy for Simultaneous Analysis of N-Glycopeptides and Phosphopeptides from Human Pancreatic Tissues

doi: 10.3791/63735

Figure Lengend Snippet: Table of Materials

Article Snippet: Potassium Phosphate Monobasic (Crystalline/Certified ACS) , Fisher Scientific , P285-500 , .

Techniques: Software, Centrifugation, Protease Inhibitor, Lysis, Blocking Assay, Transferring, Bicinchoninic Acid Protein Assay, Chromatography, Injection, Sequencing, Modification, Mass Spectrometry

Journal: Journal of visualized experiments : JoVE

Article Title: Preparation of Meiotic Chromosome Spreads from Zebrafish Spermatocytes

doi: 10.3791/60671

Figure Lengend Snippet: Table of Materials

Article Snippet: Eppendorf Tubes, 5 mL VWR International 89429–308 Formamide Fisher Scientific BP228–100 Goat anti-chicken IgY (H+L) secondary antibody, Alexa Fluor 488 ThermoFisher Scientific A-11039 Use at 1:1000 Goat anti-chicken IgY (H+L) secondary antibody, Alexa Fluor 594 ThermoFisher Scientific A-11042 Use at 1:1000 Goat anti-hDMC1 Santa Cruz Biotechnology sc-8973 Does not work in our hands Goat anti-rabbit IgG (H+L) cross-adsorbed secondary antibody, Alexa Fluor 488 ThermoFisher Scientific A-11008 Use at 1:1000 Goat anti-rabbit IgG (H+L) cross-adsorbed secondary antibody, Alexa Fluor 594 ThermoFisher Scientific A-11012 Use at 1:1000 Goat serum Sigma-Aldrich G9023–10mL Heparin sodium salt Sigma-Aldrich H3393–100KU Humidity chamber Fisher Scientific 50–112-3683 Hybridization Oven VWR International 230401V (Model 5420) Incubator Shaker New Brunswick Scientific Model Classic C25 KCl Fisher Scientific P217–500 Kimwipes Kimerbly-Clark Professional 34155 Used for the humidity chamber KH 2 PO 4 Fisher Scientific P285–500 Microscope Several commercial brands available Any standard microscope capable of at least ~1.65X magnification is sufficient.

Techniques: Generated, Modification, Hybridization, Microscopy, Electron Microscopy