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Mimetics p47 phox p22 phox inhibition
P47 Phox P22 Phox Inhibition, supplied by Mimetics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anticytochrome b245 light chain p22 phox gr3400986 1 antibody  (Danaher Inc)


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    Danaher Inc anticytochrome b245 light chain p22 phox gr3400986 1 antibody
    Anticytochrome B245 Light Chain P22 Phox Gr3400986 1 Antibody, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anticytochrome b245 light chain p22 phox gr3400986 1 antibody/product/Danaher Inc
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    p22 phox  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc p22 phox
    PCL surgery and atherosclerosis model in WT and MerTK -/- mice. ( A ) Schematic diagram for acute atherosclerosis model combined with PCL surgery and AAV8-PCSK9 injection. ( B ) Westen blotting for MerTK expression in RCA or LCA from WT mice with PCL surgery. ( C ) Immunostaining for MerTK in the straight section of thoracic aorta and aortic arch. ( D ) Immunostaining for Caspase-3 in LCA from WT mice with PCL surgery. ( E ) Representative immunostaining for cleaved IL-1β and Caspase-3 in RCA and LCA from MerTK -/- and WT mice, respectively. ( F - J ) Immunostaining for MerTK, Caspase-3, NF-kB, TLR4, vWF, VACAM-1 and <t>p22</t> <t>phox</t> in aortic arch from MerTK -/- and WT mice. Mice (n=5-7) were injected with one dose of AAV8-PCSK9, and PCL surgery was performed 1-week later. The mice were fed HFD for 4 weeks
    P22 Phox, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Disturbed flow impairs MerTK-mediated efferocytosis in aortic endothelial cells during atherosclerosis"

    Article Title: Disturbed flow impairs MerTK-mediated efferocytosis in aortic endothelial cells during atherosclerosis

    Journal: Theranostics

    doi: 10.7150/thno.93036

    PCL surgery and atherosclerosis model in WT and MerTK -/- mice. ( A ) Schematic diagram for acute atherosclerosis model combined with PCL surgery and AAV8-PCSK9 injection. ( B ) Westen blotting for MerTK expression in RCA or LCA from WT mice with PCL surgery. ( C ) Immunostaining for MerTK in the straight section of thoracic aorta and aortic arch. ( D ) Immunostaining for Caspase-3 in LCA from WT mice with PCL surgery. ( E ) Representative immunostaining for cleaved IL-1β and Caspase-3 in RCA and LCA from MerTK -/- and WT mice, respectively. ( F - J ) Immunostaining for MerTK, Caspase-3, NF-kB, TLR4, vWF, VACAM-1 and p22 phox in aortic arch from MerTK -/- and WT mice. Mice (n=5-7) were injected with one dose of AAV8-PCSK9, and PCL surgery was performed 1-week later. The mice were fed HFD for 4 weeks
    Figure Legend Snippet: PCL surgery and atherosclerosis model in WT and MerTK -/- mice. ( A ) Schematic diagram for acute atherosclerosis model combined with PCL surgery and AAV8-PCSK9 injection. ( B ) Westen blotting for MerTK expression in RCA or LCA from WT mice with PCL surgery. ( C ) Immunostaining for MerTK in the straight section of thoracic aorta and aortic arch. ( D ) Immunostaining for Caspase-3 in LCA from WT mice with PCL surgery. ( E ) Representative immunostaining for cleaved IL-1β and Caspase-3 in RCA and LCA from MerTK -/- and WT mice, respectively. ( F - J ) Immunostaining for MerTK, Caspase-3, NF-kB, TLR4, vWF, VACAM-1 and p22 phox in aortic arch from MerTK -/- and WT mice. Mice (n=5-7) were injected with one dose of AAV8-PCSK9, and PCL surgery was performed 1-week later. The mice were fed HFD for 4 weeks

    Techniques Used: Injection, Expressing, Immunostaining

    p22 phox  (Danaher Inc)


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    Danaher Inc p22 phox
    P22 Phox, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p22 phox/product/Danaher Inc
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    p22 phox - by Bioz Stars, 2024-07
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    Structured Review

    Mimetics p47 phox p22 phox inhibition
    P47 Phox P22 Phox Inhibition, supplied by Mimetics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p47 phox p22 phox inhibition/product/Mimetics
    Average 86 stars, based on 1 article reviews
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    p47 phox p22 phox inhibition - by Bioz Stars, 2024-07
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    Structured Review

    ABclonal Biotechnology p22 phox antibody
    Activated SIRT1 inhibited NOX4 activation and promoted NOX4 ubiquitination degradation. ( A ) Endothelial cells were treated with different concentrations of ECH and SIRT1 activator resveratrol. Immunofluorescence was used to assess the co-staining of NOX4 (red) and <t>p22</t> <t>phox</t> (green). Bars represent 20 μm. The microscope’s magnifications are the same. ( B ) CO-IP was performed to assess the binding of NOX4 and p22 phox. ( C ) CO-IP was performed to assess the binding of SIRT1 and p22 phox. ( D ) CO-IP was used to measure the ubiquitination level of NOX4. ( E ) CO-IP was used to measure the acetylation level of Nrf2. ( F ) Under Control siRNA and SIRT1 siRNA treatments, immunofluorescence was used to assess the co-staining of NOX4 (red) and p22 phox (green). Bars represent 20 μm. The microscope’s magnifications are the same. ( G ) Under Control siRNA and SIRT1 siRNA treatments, CO-IP was performed to assess the binding of NOX4 and p22 phox. ( H ) Under Control siRNA and SIRT1 siRNA treatments, CO-IP was performed to assess the binding of SIRT1 and p22 phox. ( I ) Under Control siRNA and SIRT1 siRNA treatments, CO-IP was used to measure the ubiquitination level of NOX4. The data are presented as mean ± standard deviation, and all experiments were repeated independently at least three times.
    P22 Phox Antibody, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p22 phox antibody/product/ABclonal Biotechnology
    Average 86 stars, based on 1 article reviews
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    p22 phox antibody - by Bioz Stars, 2024-07
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    1) Product Images from "Sirtuin1 Mediates the Protective Effects of Echinacoside against Sepsis-Induced Acute Lung Injury via Regulating the NOX4-Nrf2 Axis"

    Article Title: Sirtuin1 Mediates the Protective Effects of Echinacoside against Sepsis-Induced Acute Lung Injury via Regulating the NOX4-Nrf2 Axis

    Journal: Antioxidants

    doi: 10.3390/antiox12111925

    Activated SIRT1 inhibited NOX4 activation and promoted NOX4 ubiquitination degradation. ( A ) Endothelial cells were treated with different concentrations of ECH and SIRT1 activator resveratrol. Immunofluorescence was used to assess the co-staining of NOX4 (red) and p22 phox (green). Bars represent 20 μm. The microscope’s magnifications are the same. ( B ) CO-IP was performed to assess the binding of NOX4 and p22 phox. ( C ) CO-IP was performed to assess the binding of SIRT1 and p22 phox. ( D ) CO-IP was used to measure the ubiquitination level of NOX4. ( E ) CO-IP was used to measure the acetylation level of Nrf2. ( F ) Under Control siRNA and SIRT1 siRNA treatments, immunofluorescence was used to assess the co-staining of NOX4 (red) and p22 phox (green). Bars represent 20 μm. The microscope’s magnifications are the same. ( G ) Under Control siRNA and SIRT1 siRNA treatments, CO-IP was performed to assess the binding of NOX4 and p22 phox. ( H ) Under Control siRNA and SIRT1 siRNA treatments, CO-IP was performed to assess the binding of SIRT1 and p22 phox. ( I ) Under Control siRNA and SIRT1 siRNA treatments, CO-IP was used to measure the ubiquitination level of NOX4. The data are presented as mean ± standard deviation, and all experiments were repeated independently at least three times.
    Figure Legend Snippet: Activated SIRT1 inhibited NOX4 activation and promoted NOX4 ubiquitination degradation. ( A ) Endothelial cells were treated with different concentrations of ECH and SIRT1 activator resveratrol. Immunofluorescence was used to assess the co-staining of NOX4 (red) and p22 phox (green). Bars represent 20 μm. The microscope’s magnifications are the same. ( B ) CO-IP was performed to assess the binding of NOX4 and p22 phox. ( C ) CO-IP was performed to assess the binding of SIRT1 and p22 phox. ( D ) CO-IP was used to measure the ubiquitination level of NOX4. ( E ) CO-IP was used to measure the acetylation level of Nrf2. ( F ) Under Control siRNA and SIRT1 siRNA treatments, immunofluorescence was used to assess the co-staining of NOX4 (red) and p22 phox (green). Bars represent 20 μm. The microscope’s magnifications are the same. ( G ) Under Control siRNA and SIRT1 siRNA treatments, CO-IP was performed to assess the binding of NOX4 and p22 phox. ( H ) Under Control siRNA and SIRT1 siRNA treatments, CO-IP was performed to assess the binding of SIRT1 and p22 phox. ( I ) Under Control siRNA and SIRT1 siRNA treatments, CO-IP was used to measure the ubiquitination level of NOX4. The data are presented as mean ± standard deviation, and all experiments were repeated independently at least three times.

    Techniques Used: Activation Assay, Immunofluorescence, Staining, Co-Immunoprecipitation Assay, Binding Assay, Standard Deviation


    Structured Review

    ABclonal Biotechnology p22 phox antibody
    P22 Phox Antibody, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p22 phox antibody/product/ABclonal Biotechnology
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    p22 phox antibody - by Bioz Stars, 2024-07
    86/100 stars

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    Structured Review

    ABclonal Biotechnology p22 phox antibody
    P22 Phox Antibody, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p22 phox antibody/product/ABclonal Biotechnology
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    p22 phox antibody - by Bioz Stars, 2024-07
    86/100 stars

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    Structured Review

    ABclonal Biotechnology p22 phox antibody
    Activated SIRT1 inhibited NOX4 activation and promoted NOX4 ubiquitination degradation. ( A ) Endothelial cells were treated with different concentrations of ECH and SIRT1 activator resveratrol. Immunofluorescence was used to assess the co-staining of NOX4 (red) and <t>p22</t> <t>phox</t> (green). Bars represent 20 μm. The microscope’s magnifications are the same. ( B ) CO-IP was performed to assess the binding of NOX4 and p22 phox. ( C ) CO-IP was performed to assess the binding of SIRT1 and p22 phox. ( D ) CO-IP was used to measure the ubiquitination level of NOX4. ( E ) CO-IP was used to measure the acetylation level of Nrf2. ( F ) Under Control siRNA and SIRT1 siRNA treatments, immunofluorescence was used to assess the co-staining of NOX4 (red) and p22 phox (green). Bars represent 20 μm. The microscope’s magnifications are the same. ( G ) Under Control siRNA and SIRT1 siRNA treatments, CO-IP was performed to assess the binding of NOX4 and p22 phox. ( H ) Under Control siRNA and SIRT1 siRNA treatments, CO-IP was performed to assess the binding of SIRT1 and p22 phox. ( I ) Under Control siRNA and SIRT1 siRNA treatments, CO-IP was used to measure the ubiquitination level of NOX4. The data are presented as mean ± standard deviation, and all experiments were repeated independently at least three times.
    P22 Phox Antibody, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p22 phox antibody/product/ABclonal Biotechnology
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    p22 phox antibody - by Bioz Stars, 2024-07
    86/100 stars

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    1) Product Images from "Sirtuin1 Mediates the Protective Effects of Echinacoside against Sepsis-Induced Acute Lung Injury via Regulating the NOX4-Nrf2 Axis"

    Article Title: Sirtuin1 Mediates the Protective Effects of Echinacoside against Sepsis-Induced Acute Lung Injury via Regulating the NOX4-Nrf2 Axis

    Journal: Antioxidants

    doi: 10.3390/antiox12111925

    Activated SIRT1 inhibited NOX4 activation and promoted NOX4 ubiquitination degradation. ( A ) Endothelial cells were treated with different concentrations of ECH and SIRT1 activator resveratrol. Immunofluorescence was used to assess the co-staining of NOX4 (red) and p22 phox (green). Bars represent 20 μm. The microscope’s magnifications are the same. ( B ) CO-IP was performed to assess the binding of NOX4 and p22 phox. ( C ) CO-IP was performed to assess the binding of SIRT1 and p22 phox. ( D ) CO-IP was used to measure the ubiquitination level of NOX4. ( E ) CO-IP was used to measure the acetylation level of Nrf2. ( F ) Under Control siRNA and SIRT1 siRNA treatments, immunofluorescence was used to assess the co-staining of NOX4 (red) and p22 phox (green). Bars represent 20 μm. The microscope’s magnifications are the same. ( G ) Under Control siRNA and SIRT1 siRNA treatments, CO-IP was performed to assess the binding of NOX4 and p22 phox. ( H ) Under Control siRNA and SIRT1 siRNA treatments, CO-IP was performed to assess the binding of SIRT1 and p22 phox. ( I ) Under Control siRNA and SIRT1 siRNA treatments, CO-IP was used to measure the ubiquitination level of NOX4. The data are presented as mean ± standard deviation, and all experiments were repeated independently at least three times.
    Figure Legend Snippet: Activated SIRT1 inhibited NOX4 activation and promoted NOX4 ubiquitination degradation. ( A ) Endothelial cells were treated with different concentrations of ECH and SIRT1 activator resveratrol. Immunofluorescence was used to assess the co-staining of NOX4 (red) and p22 phox (green). Bars represent 20 μm. The microscope’s magnifications are the same. ( B ) CO-IP was performed to assess the binding of NOX4 and p22 phox. ( C ) CO-IP was performed to assess the binding of SIRT1 and p22 phox. ( D ) CO-IP was used to measure the ubiquitination level of NOX4. ( E ) CO-IP was used to measure the acetylation level of Nrf2. ( F ) Under Control siRNA and SIRT1 siRNA treatments, immunofluorescence was used to assess the co-staining of NOX4 (red) and p22 phox (green). Bars represent 20 μm. The microscope’s magnifications are the same. ( G ) Under Control siRNA and SIRT1 siRNA treatments, CO-IP was performed to assess the binding of NOX4 and p22 phox. ( H ) Under Control siRNA and SIRT1 siRNA treatments, CO-IP was performed to assess the binding of SIRT1 and p22 phox. ( I ) Under Control siRNA and SIRT1 siRNA treatments, CO-IP was used to measure the ubiquitination level of NOX4. The data are presented as mean ± standard deviation, and all experiments were repeated independently at least three times.

    Techniques Used: Activation Assay, Immunofluorescence, Staining, Co-Immunoprecipitation Assay, Binding Assay, Standard Deviation

    cho noxo1 noxa1 p22 phox cells  (Thermo Fisher)


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    Thermo Fisher cho noxo1 noxa1 p22 phox cells
    VEO-IBD NOX1 mutations abolish catalytic activity and prevent peroxynitrite formation. (A) Superoxide generation of <t>CHO-NOXO1-NOXA1-p22</t> phox cells (control) or cells co-expressing NOX1 WT, NOX1 N122H or NOX1 T497A in the presence or absence of PMA (in RLU and as % of WT). (B) Immunoblot of cell lines in (A). (C) Detection of peroxynitrite with FIBA in the absence or presence of 10 μM l -NAME (in RFU). Cell lines in (A) co-expressing iNOS as indicated were stimulated with PMA. (D) Nitrate quantification in supernatants of indicated cell lines after PMA stimulation (1 h). (E) Immunoblot of cell lines in (C). A two-way ANOVA with multiple comparisons test was performed (A) or a Kruskal-Wallis test with multiple comparisons (D). (non-significant (NS) P > 0.05, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001).
    Cho Noxo1 Noxa1 P22 Phox Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cho noxo1 noxa1 p22 phox cells/product/Thermo Fisher
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    1) Product Images from "VEO-IBD NOX1 variant highlights a structural region essential for NOX/DUOX catalytic activity"

    Article Title: VEO-IBD NOX1 variant highlights a structural region essential for NOX/DUOX catalytic activity

    Journal: Redox Biology

    doi: 10.1016/j.redox.2023.102905

    VEO-IBD NOX1 mutations abolish catalytic activity and prevent peroxynitrite formation. (A) Superoxide generation of CHO-NOXO1-NOXA1-p22 phox cells (control) or cells co-expressing NOX1 WT, NOX1 N122H or NOX1 T497A in the presence or absence of PMA (in RLU and as % of WT). (B) Immunoblot of cell lines in (A). (C) Detection of peroxynitrite with FIBA in the absence or presence of 10 μM l -NAME (in RFU). Cell lines in (A) co-expressing iNOS as indicated were stimulated with PMA. (D) Nitrate quantification in supernatants of indicated cell lines after PMA stimulation (1 h). (E) Immunoblot of cell lines in (C). A two-way ANOVA with multiple comparisons test was performed (A) or a Kruskal-Wallis test with multiple comparisons (D). (non-significant (NS) P > 0.05, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001).
    Figure Legend Snippet: VEO-IBD NOX1 mutations abolish catalytic activity and prevent peroxynitrite formation. (A) Superoxide generation of CHO-NOXO1-NOXA1-p22 phox cells (control) or cells co-expressing NOX1 WT, NOX1 N122H or NOX1 T497A in the presence or absence of PMA (in RLU and as % of WT). (B) Immunoblot of cell lines in (A). (C) Detection of peroxynitrite with FIBA in the absence or presence of 10 μM l -NAME (in RFU). Cell lines in (A) co-expressing iNOS as indicated were stimulated with PMA. (D) Nitrate quantification in supernatants of indicated cell lines after PMA stimulation (1 h). (E) Immunoblot of cell lines in (C). A two-way ANOVA with multiple comparisons test was performed (A) or a Kruskal-Wallis test with multiple comparisons (D). (non-significant (NS) P > 0.05, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001).

    Techniques Used: Activity Assay, Expressing, Western Blot

    Conserved asparagine in the HxxxHxxN motif is essential for the catalytic activity of NOX/DUOX. (A) NOX1-5 and DUOX1-2 sequence alignments with asparagine (blue) and histidines (yellow), and with threonine (blue) and GRP sequence (yellow). (B) CHO-NOXO1-NOXA1-p22 phox cell lines were transfected with NOX1 WT and mutants, followed by PMA stimulation. (C) COS7-p22 phox cells were transfected with p47 phox , p67 phox , and NOX2 WT or mutants, followed by PMA stimulation. (D) COS7-p22 phox cells were transfected with NOX4 WT or mutants, constitutive H 2 O 2 generation was measured. (E) H661-DUOXA2 cells were transfected with DUOX2 or mutants, PMA/thapsigargin stimulated H 2 O 2 generation was measured. (F) Percentage of NOX4 or DUOX2 positive cells by cell surface staining, transfections as above. A one-way ANOVA with multiple comparisons (NOX1,2) or Kruskal-Wallis test with multiple comparisons (NOX4, DUOX2) was performed (non-significant (NS) P > 0.05, *P ≤ 0.05, ****P ≤ 0.0001). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
    Figure Legend Snippet: Conserved asparagine in the HxxxHxxN motif is essential for the catalytic activity of NOX/DUOX. (A) NOX1-5 and DUOX1-2 sequence alignments with asparagine (blue) and histidines (yellow), and with threonine (blue) and GRP sequence (yellow). (B) CHO-NOXO1-NOXA1-p22 phox cell lines were transfected with NOX1 WT and mutants, followed by PMA stimulation. (C) COS7-p22 phox cells were transfected with p47 phox , p67 phox , and NOX2 WT or mutants, followed by PMA stimulation. (D) COS7-p22 phox cells were transfected with NOX4 WT or mutants, constitutive H 2 O 2 generation was measured. (E) H661-DUOXA2 cells were transfected with DUOX2 or mutants, PMA/thapsigargin stimulated H 2 O 2 generation was measured. (F) Percentage of NOX4 or DUOX2 positive cells by cell surface staining, transfections as above. A one-way ANOVA with multiple comparisons (NOX1,2) or Kruskal-Wallis test with multiple comparisons (NOX4, DUOX2) was performed (non-significant (NS) P > 0.05, *P ≤ 0.05, ****P ≤ 0.0001). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

    Techniques Used: Activity Assay, Sequencing, Transfection, Staining

    NOX1 modelling reveals structural features involved in the oxygen reduction center. (A) Cartoon representation of a NOX1/p22 phox heterodimer model inside view (left) or eclipsed (p22 phox behind, center). On the right, eclipsed view in surface mode revealing lateral access to the distal heme (zoom), NOX1 (teal blue), p22 phox (orange). (B) Zoom, on a vertical section within NOX1, on the distal heme region in NOX1 with cavities highlighted in surface mode. An additional internal pocket at the distal heme site is revealed. The side chains of the heme-chelating histidines as well as N122 and H119, delimiting the internal pocket, are represented by sticks. The vertical section within NOX1 allows to see only TM3, TM4 and partly TM5, while the others have been eliminated from front for clarity. (C) Zoom in NOX1 structure of the N122 environment at the interface between TM3 and TM2 observed from the outside (left) or the inside of NOX1 (right). (D) Modelling the structural impact of mutations at position N122. Modification performed by the use of the mutagenesis function within Pymol software. From left to right, the N122H, N122Q, N122L and N122T mutations. For the first two, several possible rotamers have been represented simultaneously for the corresponding mutated side chain. In the case of N122L and N122T modeled mutations another rotamer of the facing T49 side chain has been considered as possible adaptation to the mutation of N122. In (C) and (D), dotted yellow line represented H-bond and dotted green line possible hydrophobic interaction. (E) CHO-NOXO1-NOXA1-p22 phox cells were transfected with NOX1 WT and mutants, followed by PMA stimulation. Immunoblots indicate equal protein expression. One-way ANOVA with repeated measures (non-significant (NS) P > 0.05, *P ≤ 0.05, ****P ≤ 0.0001). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
    Figure Legend Snippet: NOX1 modelling reveals structural features involved in the oxygen reduction center. (A) Cartoon representation of a NOX1/p22 phox heterodimer model inside view (left) or eclipsed (p22 phox behind, center). On the right, eclipsed view in surface mode revealing lateral access to the distal heme (zoom), NOX1 (teal blue), p22 phox (orange). (B) Zoom, on a vertical section within NOX1, on the distal heme region in NOX1 with cavities highlighted in surface mode. An additional internal pocket at the distal heme site is revealed. The side chains of the heme-chelating histidines as well as N122 and H119, delimiting the internal pocket, are represented by sticks. The vertical section within NOX1 allows to see only TM3, TM4 and partly TM5, while the others have been eliminated from front for clarity. (C) Zoom in NOX1 structure of the N122 environment at the interface between TM3 and TM2 observed from the outside (left) or the inside of NOX1 (right). (D) Modelling the structural impact of mutations at position N122. Modification performed by the use of the mutagenesis function within Pymol software. From left to right, the N122H, N122Q, N122L and N122T mutations. For the first two, several possible rotamers have been represented simultaneously for the corresponding mutated side chain. In the case of N122L and N122T modeled mutations another rotamer of the facing T49 side chain has been considered as possible adaptation to the mutation of N122. In (C) and (D), dotted yellow line represented H-bond and dotted green line possible hydrophobic interaction. (E) CHO-NOXO1-NOXA1-p22 phox cells were transfected with NOX1 WT and mutants, followed by PMA stimulation. Immunoblots indicate equal protein expression. One-way ANOVA with repeated measures (non-significant (NS) P > 0.05, *P ≤ 0.05, ****P ≤ 0.0001). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

    Techniques Used: Modification, Mutagenesis, Software, Transfection, Western Blot, Expressing

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    Mimetics p47 phox p22 phox inhibition
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    86
    Cell Signaling Technology Inc p22 phox
    PCL surgery and atherosclerosis model in WT and MerTK -/- mice. ( A ) Schematic diagram for acute atherosclerosis model combined with PCL surgery and AAV8-PCSK9 injection. ( B ) Westen blotting for MerTK expression in RCA or LCA from WT mice with PCL surgery. ( C ) Immunostaining for MerTK in the straight section of thoracic aorta and aortic arch. ( D ) Immunostaining for Caspase-3 in LCA from WT mice with PCL surgery. ( E ) Representative immunostaining for cleaved IL-1β and Caspase-3 in RCA and LCA from MerTK -/- and WT mice, respectively. ( F - J ) Immunostaining for MerTK, Caspase-3, NF-kB, TLR4, vWF, VACAM-1 and <t>p22</t> <t>phox</t> in aortic arch from MerTK -/- and WT mice. Mice (n=5-7) were injected with one dose of AAV8-PCSK9, and PCL surgery was performed 1-week later. The mice were fed HFD for 4 weeks
    P22 Phox, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Danaher Inc p22 phox
    PCL surgery and atherosclerosis model in WT and MerTK -/- mice. ( A ) Schematic diagram for acute atherosclerosis model combined with PCL surgery and AAV8-PCSK9 injection. ( B ) Westen blotting for MerTK expression in RCA or LCA from WT mice with PCL surgery. ( C ) Immunostaining for MerTK in the straight section of thoracic aorta and aortic arch. ( D ) Immunostaining for Caspase-3 in LCA from WT mice with PCL surgery. ( E ) Representative immunostaining for cleaved IL-1β and Caspase-3 in RCA and LCA from MerTK -/- and WT mice, respectively. ( F - J ) Immunostaining for MerTK, Caspase-3, NF-kB, TLR4, vWF, VACAM-1 and <t>p22</t> <t>phox</t> in aortic arch from MerTK -/- and WT mice. Mice (n=5-7) were injected with one dose of AAV8-PCSK9, and PCL surgery was performed 1-week later. The mice were fed HFD for 4 weeks
    P22 Phox, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ABclonal Biotechnology p22 phox antibody
    Activated SIRT1 inhibited NOX4 activation and promoted NOX4 ubiquitination degradation. ( A ) Endothelial cells were treated with different concentrations of ECH and SIRT1 activator resveratrol. Immunofluorescence was used to assess the co-staining of NOX4 (red) and <t>p22</t> <t>phox</t> (green). Bars represent 20 μm. The microscope’s magnifications are the same. ( B ) CO-IP was performed to assess the binding of NOX4 and p22 phox. ( C ) CO-IP was performed to assess the binding of SIRT1 and p22 phox. ( D ) CO-IP was used to measure the ubiquitination level of NOX4. ( E ) CO-IP was used to measure the acetylation level of Nrf2. ( F ) Under Control siRNA and SIRT1 siRNA treatments, immunofluorescence was used to assess the co-staining of NOX4 (red) and p22 phox (green). Bars represent 20 μm. The microscope’s magnifications are the same. ( G ) Under Control siRNA and SIRT1 siRNA treatments, CO-IP was performed to assess the binding of NOX4 and p22 phox. ( H ) Under Control siRNA and SIRT1 siRNA treatments, CO-IP was performed to assess the binding of SIRT1 and p22 phox. ( I ) Under Control siRNA and SIRT1 siRNA treatments, CO-IP was used to measure the ubiquitination level of NOX4. The data are presented as mean ± standard deviation, and all experiments were repeated independently at least three times.
    P22 Phox Antibody, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher cho noxo1 noxa1 p22 phox cells
    VEO-IBD NOX1 mutations abolish catalytic activity and prevent peroxynitrite formation. (A) Superoxide generation of <t>CHO-NOXO1-NOXA1-p22</t> phox cells (control) or cells co-expressing NOX1 WT, NOX1 N122H or NOX1 T497A in the presence or absence of PMA (in RLU and as % of WT). (B) Immunoblot of cell lines in (A). (C) Detection of peroxynitrite with FIBA in the absence or presence of 10 μM l -NAME (in RFU). Cell lines in (A) co-expressing iNOS as indicated were stimulated with PMA. (D) Nitrate quantification in supernatants of indicated cell lines after PMA stimulation (1 h). (E) Immunoblot of cell lines in (C). A two-way ANOVA with multiple comparisons test was performed (A) or a Kruskal-Wallis test with multiple comparisons (D). (non-significant (NS) P > 0.05, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001).
    Cho Noxo1 Noxa1 P22 Phox Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    PCL surgery and atherosclerosis model in WT and MerTK -/- mice. ( A ) Schematic diagram for acute atherosclerosis model combined with PCL surgery and AAV8-PCSK9 injection. ( B ) Westen blotting for MerTK expression in RCA or LCA from WT mice with PCL surgery. ( C ) Immunostaining for MerTK in the straight section of thoracic aorta and aortic arch. ( D ) Immunostaining for Caspase-3 in LCA from WT mice with PCL surgery. ( E ) Representative immunostaining for cleaved IL-1β and Caspase-3 in RCA and LCA from MerTK -/- and WT mice, respectively. ( F - J ) Immunostaining for MerTK, Caspase-3, NF-kB, TLR4, vWF, VACAM-1 and p22 phox in aortic arch from MerTK -/- and WT mice. Mice (n=5-7) were injected with one dose of AAV8-PCSK9, and PCL surgery was performed 1-week later. The mice were fed HFD for 4 weeks

    Journal: Theranostics

    Article Title: Disturbed flow impairs MerTK-mediated efferocytosis in aortic endothelial cells during atherosclerosis

    doi: 10.7150/thno.93036

    Figure Lengend Snippet: PCL surgery and atherosclerosis model in WT and MerTK -/- mice. ( A ) Schematic diagram for acute atherosclerosis model combined with PCL surgery and AAV8-PCSK9 injection. ( B ) Westen blotting for MerTK expression in RCA or LCA from WT mice with PCL surgery. ( C ) Immunostaining for MerTK in the straight section of thoracic aorta and aortic arch. ( D ) Immunostaining for Caspase-3 in LCA from WT mice with PCL surgery. ( E ) Representative immunostaining for cleaved IL-1β and Caspase-3 in RCA and LCA from MerTK -/- and WT mice, respectively. ( F - J ) Immunostaining for MerTK, Caspase-3, NF-kB, TLR4, vWF, VACAM-1 and p22 phox in aortic arch from MerTK -/- and WT mice. Mice (n=5-7) were injected with one dose of AAV8-PCSK9, and PCL surgery was performed 1-week later. The mice were fed HFD for 4 weeks

    Article Snippet: The information of antibodies is shown as follows: cleaved IL-1β (Cell Signaling), Caspase-3 (Santa Cruz), Phospho-p70s6k (Cell Signaling), Phospho-EIF2α (Cell Signaling), EIF3A (Cell Signaling), vWF (Abcam), VCAM-1 (Cell Signaling) and p22 phox (Cell Signaling).

    Techniques: Injection, Expressing, Immunostaining

    Activated SIRT1 inhibited NOX4 activation and promoted NOX4 ubiquitination degradation. ( A ) Endothelial cells were treated with different concentrations of ECH and SIRT1 activator resveratrol. Immunofluorescence was used to assess the co-staining of NOX4 (red) and p22 phox (green). Bars represent 20 μm. The microscope’s magnifications are the same. ( B ) CO-IP was performed to assess the binding of NOX4 and p22 phox. ( C ) CO-IP was performed to assess the binding of SIRT1 and p22 phox. ( D ) CO-IP was used to measure the ubiquitination level of NOX4. ( E ) CO-IP was used to measure the acetylation level of Nrf2. ( F ) Under Control siRNA and SIRT1 siRNA treatments, immunofluorescence was used to assess the co-staining of NOX4 (red) and p22 phox (green). Bars represent 20 μm. The microscope’s magnifications are the same. ( G ) Under Control siRNA and SIRT1 siRNA treatments, CO-IP was performed to assess the binding of NOX4 and p22 phox. ( H ) Under Control siRNA and SIRT1 siRNA treatments, CO-IP was performed to assess the binding of SIRT1 and p22 phox. ( I ) Under Control siRNA and SIRT1 siRNA treatments, CO-IP was used to measure the ubiquitination level of NOX4. The data are presented as mean ± standard deviation, and all experiments were repeated independently at least three times.

    Journal: Antioxidants

    Article Title: Sirtuin1 Mediates the Protective Effects of Echinacoside against Sepsis-Induced Acute Lung Injury via Regulating the NOX4-Nrf2 Axis

    doi: 10.3390/antiox12111925

    Figure Lengend Snippet: Activated SIRT1 inhibited NOX4 activation and promoted NOX4 ubiquitination degradation. ( A ) Endothelial cells were treated with different concentrations of ECH and SIRT1 activator resveratrol. Immunofluorescence was used to assess the co-staining of NOX4 (red) and p22 phox (green). Bars represent 20 μm. The microscope’s magnifications are the same. ( B ) CO-IP was performed to assess the binding of NOX4 and p22 phox. ( C ) CO-IP was performed to assess the binding of SIRT1 and p22 phox. ( D ) CO-IP was used to measure the ubiquitination level of NOX4. ( E ) CO-IP was used to measure the acetylation level of Nrf2. ( F ) Under Control siRNA and SIRT1 siRNA treatments, immunofluorescence was used to assess the co-staining of NOX4 (red) and p22 phox (green). Bars represent 20 μm. The microscope’s magnifications are the same. ( G ) Under Control siRNA and SIRT1 siRNA treatments, CO-IP was performed to assess the binding of NOX4 and p22 phox. ( H ) Under Control siRNA and SIRT1 siRNA treatments, CO-IP was performed to assess the binding of SIRT1 and p22 phox. ( I ) Under Control siRNA and SIRT1 siRNA treatments, CO-IP was used to measure the ubiquitination level of NOX4. The data are presented as mean ± standard deviation, and all experiments were repeated independently at least three times.

    Article Snippet: The membranes were then incubated with specific primary antibodies overnight at 4 °C, including β-actin antibody (1:5000, Cat# 66009-1-Ig, Proteintech, China), VCAM-1 antibody (1:1000, Cat# bs-0920R, Bioss, Boston, MA, USA), ICAM-1 antibody (1:1000, Cat# 10020-1-AP, Proteintech, China), SIRT1 polyclonal antibody (1:1000, CAT# 13161-1-AP, Proteintech, China), HO-1 antibody (1:2000, Cat# ab189491, Abcam, Cambridge, UK), NQO-1 antibody (1:2000, CAT# ab80588, Abcam, UK), NOX4 antibody (1:1000, Cat# 14347-1-AP, Proteintech, China), p65 antibody (1:1000, Cat# 8242T, CST, Danvers, MA, USA), Pho-p65 antibody (1:1000, Cat# 3033T, CST, USA), IκB α antibody (1:1000, Cat# ab32518, Abcam, UK), Pho-IκB α antibody (1:10,000, Cat# ab133462, Abcam, UK), Pho-JNK antibody (1:1000, Cat# 9251S, CST, USA), JNK antibody (1:1000, Cat# 9252T, CST, USA), ERK1/2 antibody (1:1000, Cat# 4695T, CST, USA), Pho-ERK1/2 antibody (1:1000, Cat# 4370T, CST, USA), Pho-p38 antibody (1:1000, Cat# 28796-1-AP, Proteintech, China), p38 antibody (1:1000, Cat# 14064-1-AP, Proteintech, China), Nrf2 antibody (1:1000, Cat# 16396-1-AP, Proteintech, China), Ubquination antibody (1:500, Cat# A162, Abclonal, Wuhan, China) and p22 phox antibody (1:1000, Cat# A10694, ABclonal, China).

    Techniques: Activation Assay, Immunofluorescence, Staining, Co-Immunoprecipitation Assay, Binding Assay, Standard Deviation

    VEO-IBD NOX1 mutations abolish catalytic activity and prevent peroxynitrite formation. (A) Superoxide generation of CHO-NOXO1-NOXA1-p22 phox cells (control) or cells co-expressing NOX1 WT, NOX1 N122H or NOX1 T497A in the presence or absence of PMA (in RLU and as % of WT). (B) Immunoblot of cell lines in (A). (C) Detection of peroxynitrite with FIBA in the absence or presence of 10 μM l -NAME (in RFU). Cell lines in (A) co-expressing iNOS as indicated were stimulated with PMA. (D) Nitrate quantification in supernatants of indicated cell lines after PMA stimulation (1 h). (E) Immunoblot of cell lines in (C). A two-way ANOVA with multiple comparisons test was performed (A) or a Kruskal-Wallis test with multiple comparisons (D). (non-significant (NS) P > 0.05, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001).

    Journal: Redox Biology

    Article Title: VEO-IBD NOX1 variant highlights a structural region essential for NOX/DUOX catalytic activity

    doi: 10.1016/j.redox.2023.102905

    Figure Lengend Snippet: VEO-IBD NOX1 mutations abolish catalytic activity and prevent peroxynitrite formation. (A) Superoxide generation of CHO-NOXO1-NOXA1-p22 phox cells (control) or cells co-expressing NOX1 WT, NOX1 N122H or NOX1 T497A in the presence or absence of PMA (in RLU and as % of WT). (B) Immunoblot of cell lines in (A). (C) Detection of peroxynitrite with FIBA in the absence or presence of 10 μM l -NAME (in RFU). Cell lines in (A) co-expressing iNOS as indicated were stimulated with PMA. (D) Nitrate quantification in supernatants of indicated cell lines after PMA stimulation (1 h). (E) Immunoblot of cell lines in (C). A two-way ANOVA with multiple comparisons test was performed (A) or a Kruskal-Wallis test with multiple comparisons (D). (non-significant (NS) P > 0.05, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001).

    Article Snippet: CHO-NOXO1-NOXA1-p22 phox cells and COS7-p22 phox cells [ ] were transfected with Lipofectamine 3000 (Invitrogen), H661-DUOXA2 cells [ ] with Fugene (Promega).

    Techniques: Activity Assay, Expressing, Western Blot

    Conserved asparagine in the HxxxHxxN motif is essential for the catalytic activity of NOX/DUOX. (A) NOX1-5 and DUOX1-2 sequence alignments with asparagine (blue) and histidines (yellow), and with threonine (blue) and GRP sequence (yellow). (B) CHO-NOXO1-NOXA1-p22 phox cell lines were transfected with NOX1 WT and mutants, followed by PMA stimulation. (C) COS7-p22 phox cells were transfected with p47 phox , p67 phox , and NOX2 WT or mutants, followed by PMA stimulation. (D) COS7-p22 phox cells were transfected with NOX4 WT or mutants, constitutive H 2 O 2 generation was measured. (E) H661-DUOXA2 cells were transfected with DUOX2 or mutants, PMA/thapsigargin stimulated H 2 O 2 generation was measured. (F) Percentage of NOX4 or DUOX2 positive cells by cell surface staining, transfections as above. A one-way ANOVA with multiple comparisons (NOX1,2) or Kruskal-Wallis test with multiple comparisons (NOX4, DUOX2) was performed (non-significant (NS) P > 0.05, *P ≤ 0.05, ****P ≤ 0.0001). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

    Journal: Redox Biology

    Article Title: VEO-IBD NOX1 variant highlights a structural region essential for NOX/DUOX catalytic activity

    doi: 10.1016/j.redox.2023.102905

    Figure Lengend Snippet: Conserved asparagine in the HxxxHxxN motif is essential for the catalytic activity of NOX/DUOX. (A) NOX1-5 and DUOX1-2 sequence alignments with asparagine (blue) and histidines (yellow), and with threonine (blue) and GRP sequence (yellow). (B) CHO-NOXO1-NOXA1-p22 phox cell lines were transfected with NOX1 WT and mutants, followed by PMA stimulation. (C) COS7-p22 phox cells were transfected with p47 phox , p67 phox , and NOX2 WT or mutants, followed by PMA stimulation. (D) COS7-p22 phox cells were transfected with NOX4 WT or mutants, constitutive H 2 O 2 generation was measured. (E) H661-DUOXA2 cells were transfected with DUOX2 or mutants, PMA/thapsigargin stimulated H 2 O 2 generation was measured. (F) Percentage of NOX4 or DUOX2 positive cells by cell surface staining, transfections as above. A one-way ANOVA with multiple comparisons (NOX1,2) or Kruskal-Wallis test with multiple comparisons (NOX4, DUOX2) was performed (non-significant (NS) P > 0.05, *P ≤ 0.05, ****P ≤ 0.0001). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

    Article Snippet: CHO-NOXO1-NOXA1-p22 phox cells and COS7-p22 phox cells [ ] were transfected with Lipofectamine 3000 (Invitrogen), H661-DUOXA2 cells [ ] with Fugene (Promega).

    Techniques: Activity Assay, Sequencing, Transfection, Staining

    NOX1 modelling reveals structural features involved in the oxygen reduction center. (A) Cartoon representation of a NOX1/p22 phox heterodimer model inside view (left) or eclipsed (p22 phox behind, center). On the right, eclipsed view in surface mode revealing lateral access to the distal heme (zoom), NOX1 (teal blue), p22 phox (orange). (B) Zoom, on a vertical section within NOX1, on the distal heme region in NOX1 with cavities highlighted in surface mode. An additional internal pocket at the distal heme site is revealed. The side chains of the heme-chelating histidines as well as N122 and H119, delimiting the internal pocket, are represented by sticks. The vertical section within NOX1 allows to see only TM3, TM4 and partly TM5, while the others have been eliminated from front for clarity. (C) Zoom in NOX1 structure of the N122 environment at the interface between TM3 and TM2 observed from the outside (left) or the inside of NOX1 (right). (D) Modelling the structural impact of mutations at position N122. Modification performed by the use of the mutagenesis function within Pymol software. From left to right, the N122H, N122Q, N122L and N122T mutations. For the first two, several possible rotamers have been represented simultaneously for the corresponding mutated side chain. In the case of N122L and N122T modeled mutations another rotamer of the facing T49 side chain has been considered as possible adaptation to the mutation of N122. In (C) and (D), dotted yellow line represented H-bond and dotted green line possible hydrophobic interaction. (E) CHO-NOXO1-NOXA1-p22 phox cells were transfected with NOX1 WT and mutants, followed by PMA stimulation. Immunoblots indicate equal protein expression. One-way ANOVA with repeated measures (non-significant (NS) P > 0.05, *P ≤ 0.05, ****P ≤ 0.0001). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

    Journal: Redox Biology

    Article Title: VEO-IBD NOX1 variant highlights a structural region essential for NOX/DUOX catalytic activity

    doi: 10.1016/j.redox.2023.102905

    Figure Lengend Snippet: NOX1 modelling reveals structural features involved in the oxygen reduction center. (A) Cartoon representation of a NOX1/p22 phox heterodimer model inside view (left) or eclipsed (p22 phox behind, center). On the right, eclipsed view in surface mode revealing lateral access to the distal heme (zoom), NOX1 (teal blue), p22 phox (orange). (B) Zoom, on a vertical section within NOX1, on the distal heme region in NOX1 with cavities highlighted in surface mode. An additional internal pocket at the distal heme site is revealed. The side chains of the heme-chelating histidines as well as N122 and H119, delimiting the internal pocket, are represented by sticks. The vertical section within NOX1 allows to see only TM3, TM4 and partly TM5, while the others have been eliminated from front for clarity. (C) Zoom in NOX1 structure of the N122 environment at the interface between TM3 and TM2 observed from the outside (left) or the inside of NOX1 (right). (D) Modelling the structural impact of mutations at position N122. Modification performed by the use of the mutagenesis function within Pymol software. From left to right, the N122H, N122Q, N122L and N122T mutations. For the first two, several possible rotamers have been represented simultaneously for the corresponding mutated side chain. In the case of N122L and N122T modeled mutations another rotamer of the facing T49 side chain has been considered as possible adaptation to the mutation of N122. In (C) and (D), dotted yellow line represented H-bond and dotted green line possible hydrophobic interaction. (E) CHO-NOXO1-NOXA1-p22 phox cells were transfected with NOX1 WT and mutants, followed by PMA stimulation. Immunoblots indicate equal protein expression. One-way ANOVA with repeated measures (non-significant (NS) P > 0.05, *P ≤ 0.05, ****P ≤ 0.0001). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

    Article Snippet: CHO-NOXO1-NOXA1-p22 phox cells and COS7-p22 phox cells [ ] were transfected with Lipofectamine 3000 (Invitrogen), H661-DUOXA2 cells [ ] with Fugene (Promega).

    Techniques: Modification, Mutagenesis, Software, Transfection, Western Blot, Expressing