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Anti P190rhogap Monoclonal Antibody, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti p190rhogap monoclonal antibody - by Bioz Stars, 2024-04
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Becton Dickinson anti p190rhogap monoclonal antibody
Anti P190rhogap Monoclonal Antibody, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti p190rhogap monoclonal antibody - by Bioz Stars, 2024-04
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Becton Dickinson p190rhogap immunoprecipitations
P190rhogap Immunoprecipitations, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Becton Dickinson p190rhogap immunoprecipitations
P190rhogap Immunoprecipitations, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/p190rhogap immunoprecipitations/product/Becton Dickinson
Average 86 stars, based on 1 article reviews
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Becton Dickinson p190rhogap
P190rhogap, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/p190rhogap/product/Becton Dickinson
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p190rhogap - by Bioz Stars, 2024-04
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Structured Review

Becton Dickinson p190rhogap
P190rhogap, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/p190rhogap/product/Becton Dickinson
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Becton Dickinson mouse monoclonal anti p190rhogap
Mouse Monoclonal Anti P190rhogap, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse monoclonal anti p190rhogap - by Bioz Stars, 2024-04
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Becton Dickinson anti p190rhogap
Suppression of Rho signaling by FAK is crucial for podosome rosette assembly. (A) The levels of active (GTP-bound) Rho family proteins including RhoA, Rac1, and Cdc42 in the cells were measured. The results shown are representative of three experiments. (B) v-Src–transformed MEFs expressing shRNA to luciferase (shLuc) were treated with TAT-RhoV14 at various concentrations for 12 h. v-Src–transformed MEFs expressing shRNA to FAK (shFAK) were treated with TAT-C3 at various concentrations for 12 h. The formation of podosome rosettes was measured and expressed as a percentage relative to that of control cells expressing shLuc. *, P < 0.005 (compared with the shFAK cells without TAT-C3 treatment); # , P < 0.005 (compared with the shLuc cells without TAT-RhoV14 treatment). (C) Src-transformed 3T3 cells were stained for F-actin, <t>p190RhoGAP</t> (p190A), and FAK. The z stack images were obtained and reconstituted by confocal microscopy. The XY and XZ sections of the boxed area containing a podosome rosette are shown. (D) v-Src–transformed MEFs and those expressing shRNAs specific to FAK, p190RhoGAP (shp190A), or luciferase were analyzed for the expression of FAK and p190RhoGAP by immunoblotting. The level of active RhoA was measured. The formation of podosome rosettes was measured and expressed as a percentage relative to that of the control cells expressing shLuc. *, P < 0.005. (B and D) Values (means ± SD) are from three independent experiments. (E) The effect of p130Cas knockdown on Rho activity was analyzed. The level of active RhoA was measured, quantified, and expressed as fold relative to the control cells. The results shown are the representative of three experiments.
Anti P190rhogap, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti p190rhogap/product/Becton Dickinson
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
anti p190rhogap - by Bioz Stars, 2024-04
86/100 stars

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1) Product Images from "FAK is required for the assembly of podosome rosettes"

Article Title: FAK is required for the assembly of podosome rosettes

Journal: The Journal of Cell Biology

doi: 10.1083/jcb.201103016

Suppression of Rho signaling by FAK is crucial for podosome rosette assembly. (A) The levels of active (GTP-bound) Rho family proteins including RhoA, Rac1, and Cdc42 in the cells were measured. The results shown are representative of three experiments. (B) v-Src–transformed MEFs expressing shRNA to luciferase (shLuc) were treated with TAT-RhoV14 at various concentrations for 12 h. v-Src–transformed MEFs expressing shRNA to FAK (shFAK) were treated with TAT-C3 at various concentrations for 12 h. The formation of podosome rosettes was measured and expressed as a percentage relative to that of control cells expressing shLuc. *, P < 0.005 (compared with the shFAK cells without TAT-C3 treatment); # , P < 0.005 (compared with the shLuc cells without TAT-RhoV14 treatment). (C) Src-transformed 3T3 cells were stained for F-actin, p190RhoGAP (p190A), and FAK. The z stack images were obtained and reconstituted by confocal microscopy. The XY and XZ sections of the boxed area containing a podosome rosette are shown. (D) v-Src–transformed MEFs and those expressing shRNAs specific to FAK, p190RhoGAP (shp190A), or luciferase were analyzed for the expression of FAK and p190RhoGAP by immunoblotting. The level of active RhoA was measured. The formation of podosome rosettes was measured and expressed as a percentage relative to that of the control cells expressing shLuc. *, P < 0.005. (B and D) Values (means ± SD) are from three independent experiments. (E) The effect of p130Cas knockdown on Rho activity was analyzed. The level of active RhoA was measured, quantified, and expressed as fold relative to the control cells. The results shown are the representative of three experiments.
Figure Legend Snippet: Suppression of Rho signaling by FAK is crucial for podosome rosette assembly. (A) The levels of active (GTP-bound) Rho family proteins including RhoA, Rac1, and Cdc42 in the cells were measured. The results shown are representative of three experiments. (B) v-Src–transformed MEFs expressing shRNA to luciferase (shLuc) were treated with TAT-RhoV14 at various concentrations for 12 h. v-Src–transformed MEFs expressing shRNA to FAK (shFAK) were treated with TAT-C3 at various concentrations for 12 h. The formation of podosome rosettes was measured and expressed as a percentage relative to that of control cells expressing shLuc. *, P < 0.005 (compared with the shFAK cells without TAT-C3 treatment); # , P < 0.005 (compared with the shLuc cells without TAT-RhoV14 treatment). (C) Src-transformed 3T3 cells were stained for F-actin, p190RhoGAP (p190A), and FAK. The z stack images were obtained and reconstituted by confocal microscopy. The XY and XZ sections of the boxed area containing a podosome rosette are shown. (D) v-Src–transformed MEFs and those expressing shRNAs specific to FAK, p190RhoGAP (shp190A), or luciferase were analyzed for the expression of FAK and p190RhoGAP by immunoblotting. The level of active RhoA was measured. The formation of podosome rosettes was measured and expressed as a percentage relative to that of the control cells expressing shLuc. *, P < 0.005. (B and D) Values (means ± SD) are from three independent experiments. (E) The effect of p130Cas knockdown on Rho activity was analyzed. The level of active RhoA was measured, quantified, and expressed as fold relative to the control cells. The results shown are the representative of three experiments.

Techniques Used: Transformation Assay, Expressing, shRNA, Luciferase, Staining, Confocal Microscopy, Western Blot, Activity Assay


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Becton Dickinson p190rhogap
P190rhogap, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson anti p190rhogap ab
    Anti P190rhogap Ab, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti p190rhogap ab/product/Becton Dickinson
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti p190rhogap ab - by Bioz Stars, 2024-04
    86/100 stars
      Buy from Supplier

    86
    Becton Dickinson anti p190rhogap monoclonal antibody
    Anti P190rhogap Monoclonal Antibody, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti p190rhogap monoclonal antibody/product/Becton Dickinson
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti p190rhogap monoclonal antibody - by Bioz Stars, 2024-04
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      Buy from Supplier

    86
    Becton Dickinson p190rhogap immunoprecipitations
    P190rhogap Immunoprecipitations, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p190rhogap immunoprecipitations/product/Becton Dickinson
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    p190rhogap immunoprecipitations - by Bioz Stars, 2024-04
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    P190rhogap, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p190rhogap/product/Becton Dickinson
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    Becton Dickinson mouse monoclonal anti p190rhogap
    Mouse Monoclonal Anti P190rhogap, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal anti p190rhogap/product/Becton Dickinson
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    Becton Dickinson anti p190rhogap
    Suppression of Rho signaling by FAK is crucial for podosome rosette assembly. (A) The levels of active (GTP-bound) Rho family proteins including RhoA, Rac1, and Cdc42 in the cells were measured. The results shown are representative of three experiments. (B) v-Src–transformed MEFs expressing shRNA to luciferase (shLuc) were treated with TAT-RhoV14 at various concentrations for 12 h. v-Src–transformed MEFs expressing shRNA to FAK (shFAK) were treated with TAT-C3 at various concentrations for 12 h. The formation of podosome rosettes was measured and expressed as a percentage relative to that of control cells expressing shLuc. *, P < 0.005 (compared with the shFAK cells without TAT-C3 treatment); # , P < 0.005 (compared with the shLuc cells without TAT-RhoV14 treatment). (C) Src-transformed 3T3 cells were stained for F-actin, <t>p190RhoGAP</t> (p190A), and FAK. The z stack images were obtained and reconstituted by confocal microscopy. The XY and XZ sections of the boxed area containing a podosome rosette are shown. (D) v-Src–transformed MEFs and those expressing shRNAs specific to FAK, p190RhoGAP (shp190A), or luciferase were analyzed for the expression of FAK and p190RhoGAP by immunoblotting. The level of active RhoA was measured. The formation of podosome rosettes was measured and expressed as a percentage relative to that of the control cells expressing shLuc. *, P < 0.005. (B and D) Values (means ± SD) are from three independent experiments. (E) The effect of p130Cas knockdown on Rho activity was analyzed. The level of active RhoA was measured, quantified, and expressed as fold relative to the control cells. The results shown are the representative of three experiments.
    Anti P190rhogap, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti p190rhogap/product/Becton Dickinson
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti p190rhogap - by Bioz Stars, 2024-04
    86/100 stars
      Buy from Supplier

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    Suppression of Rho signaling by FAK is crucial for podosome rosette assembly. (A) The levels of active (GTP-bound) Rho family proteins including RhoA, Rac1, and Cdc42 in the cells were measured. The results shown are representative of three experiments. (B) v-Src–transformed MEFs expressing shRNA to luciferase (shLuc) were treated with TAT-RhoV14 at various concentrations for 12 h. v-Src–transformed MEFs expressing shRNA to FAK (shFAK) were treated with TAT-C3 at various concentrations for 12 h. The formation of podosome rosettes was measured and expressed as a percentage relative to that of control cells expressing shLuc. *, P < 0.005 (compared with the shFAK cells without TAT-C3 treatment); # , P < 0.005 (compared with the shLuc cells without TAT-RhoV14 treatment). (C) Src-transformed 3T3 cells were stained for F-actin, p190RhoGAP (p190A), and FAK. The z stack images were obtained and reconstituted by confocal microscopy. The XY and XZ sections of the boxed area containing a podosome rosette are shown. (D) v-Src–transformed MEFs and those expressing shRNAs specific to FAK, p190RhoGAP (shp190A), or luciferase were analyzed for the expression of FAK and p190RhoGAP by immunoblotting. The level of active RhoA was measured. The formation of podosome rosettes was measured and expressed as a percentage relative to that of the control cells expressing shLuc. *, P < 0.005. (B and D) Values (means ± SD) are from three independent experiments. (E) The effect of p130Cas knockdown on Rho activity was analyzed. The level of active RhoA was measured, quantified, and expressed as fold relative to the control cells. The results shown are the representative of three experiments.

    Journal: The Journal of Cell Biology

    Article Title: FAK is required for the assembly of podosome rosettes

    doi: 10.1083/jcb.201103016

    Figure Lengend Snippet: Suppression of Rho signaling by FAK is crucial for podosome rosette assembly. (A) The levels of active (GTP-bound) Rho family proteins including RhoA, Rac1, and Cdc42 in the cells were measured. The results shown are representative of three experiments. (B) v-Src–transformed MEFs expressing shRNA to luciferase (shLuc) were treated with TAT-RhoV14 at various concentrations for 12 h. v-Src–transformed MEFs expressing shRNA to FAK (shFAK) were treated with TAT-C3 at various concentrations for 12 h. The formation of podosome rosettes was measured and expressed as a percentage relative to that of control cells expressing shLuc. *, P < 0.005 (compared with the shFAK cells without TAT-C3 treatment); # , P < 0.005 (compared with the shLuc cells without TAT-RhoV14 treatment). (C) Src-transformed 3T3 cells were stained for F-actin, p190RhoGAP (p190A), and FAK. The z stack images were obtained and reconstituted by confocal microscopy. The XY and XZ sections of the boxed area containing a podosome rosette are shown. (D) v-Src–transformed MEFs and those expressing shRNAs specific to FAK, p190RhoGAP (shp190A), or luciferase were analyzed for the expression of FAK and p190RhoGAP by immunoblotting. The level of active RhoA was measured. The formation of podosome rosettes was measured and expressed as a percentage relative to that of the control cells expressing shLuc. *, P < 0.005. (B and D) Values (means ± SD) are from three independent experiments. (E) The effect of p130Cas knockdown on Rho activity was analyzed. The level of active RhoA was measured, quantified, and expressed as fold relative to the control cells. The results shown are the representative of three experiments.

    Article Snippet: Monoclonal anti-FAK (clone 77), anti-p130Cas, anti-PYK2, antipaxillin, anti-p190RhoGAP, antiphosphotyrosine (PY20), anti-Rac1, and anti-RhoA antibodies and Matrigel were purchased from BD.

    Techniques: Transformation Assay, Expressing, shRNA, Luciferase, Staining, Confocal Microscopy, Western Blot, Activity Assay