Article Title: AUTEN-67, an autophagy-enhancing drug candidate with potent antiaging and neuroprotective effects
Figure Lengend Snippet: The role of MTMR14 in autophagy control. Formation of the autophagosome membrane depends on the infiltration of phosphatidylinositol-3 phosphate (PtdIns3P) into the growing membrane structure. PtdIns3P is converted from the substrate PtdIns by the class
Article Snippet: The biochemical assay consisted of purified MTMR14 protein (OriGene Technologies, TP300809) (37 ng/reaction), 100 µM phosphatidylinositol 3-phosphate (Echelon Biosciences, P-3008a) in 25 mM Tris-HCl, pH 6 buffer (Molar Chemicals, 09350-101-190) containing 2 mM DTT (Sigma, 17-1318-01) in 10-µl total volume.