lbpa standard  (Echelon Biosciences)


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    Structured Review

    Echelon Biosciences lbpa standard
    A: HeLa cells were labeled with [32P]Pi for 24 h, chased for 14 h, and then treated or not with 0.7 mM HPCD (as in Fig. 1). Lipids were extracted, separated by 2D-TLC, and visualized by autoradiography. The position of <t>LBPA</t> (arrow) was identified <t>by</t> <t>comigration</t> with pure synthetic LBPA as standard. Right: LBPA quantified in 2D-TLCs at different time after HPCD treatment and expressed as a percentage of total 32P-phospholipids (PL). B: Cells labeled as in A were treated or not with 0.7 mM HPCD for 1 h. The lipids were extracted from medium and separated by 2D-TLC. Right shows the quantification of LBPA and total phospholipids in medium. Data are expressed as a percentage of total cellular LBPA or phospholipids, extracted, and quantified in the same experiments.
    Lbpa Standard, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lbpa standard/product/Echelon Biosciences
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lbpa standard - by Bioz Stars, 2023-02
    91/100 stars

    Images

    1) Product Images from "Cyclodextrin triggers MCOLN1-dependent endo-lysosome secretion in Niemann-Pick type C cells"

    Article Title: Cyclodextrin triggers MCOLN1-dependent endo-lysosome secretion in Niemann-Pick type C cells

    Journal: Journal of Lipid Research

    doi: 10.1194/jlr.M089979

    A: HeLa cells were labeled with [32P]Pi for 24 h, chased for 14 h, and then treated or not with 0.7 mM HPCD (as in Fig. 1). Lipids were extracted, separated by 2D-TLC, and visualized by autoradiography. The position of LBPA (arrow) was identified by comigration with pure synthetic LBPA as standard. Right: LBPA quantified in 2D-TLCs at different time after HPCD treatment and expressed as a percentage of total 32P-phospholipids (PL). B: Cells labeled as in A were treated or not with 0.7 mM HPCD for 1 h. The lipids were extracted from medium and separated by 2D-TLC. Right shows the quantification of LBPA and total phospholipids in medium. Data are expressed as a percentage of total cellular LBPA or phospholipids, extracted, and quantified in the same experiments.
    Figure Legend Snippet: A: HeLa cells were labeled with [32P]Pi for 24 h, chased for 14 h, and then treated or not with 0.7 mM HPCD (as in Fig. 1). Lipids were extracted, separated by 2D-TLC, and visualized by autoradiography. The position of LBPA (arrow) was identified by comigration with pure synthetic LBPA as standard. Right: LBPA quantified in 2D-TLCs at different time after HPCD treatment and expressed as a percentage of total 32P-phospholipids (PL). B: Cells labeled as in A were treated or not with 0.7 mM HPCD for 1 h. The lipids were extracted from medium and separated by 2D-TLC. Right shows the quantification of LBPA and total phospholipids in medium. Data are expressed as a percentage of total cellular LBPA or phospholipids, extracted, and quantified in the same experiments.

    Techniques Used: Labeling, Autoradiography

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    Echelon Biosciences lbpa standard
    A: HeLa cells were labeled with [32P]Pi for 24 h, chased for 14 h, and then treated or not with 0.7 mM HPCD (as in Fig. 1). Lipids were extracted, separated by 2D-TLC, and visualized by autoradiography. The position of <t>LBPA</t> (arrow) was identified <t>by</t> <t>comigration</t> with pure synthetic LBPA as standard. Right: LBPA quantified in 2D-TLCs at different time after HPCD treatment and expressed as a percentage of total 32P-phospholipids (PL). B: Cells labeled as in A were treated or not with 0.7 mM HPCD for 1 h. The lipids were extracted from medium and separated by 2D-TLC. Right shows the quantification of LBPA and total phospholipids in medium. Data are expressed as a percentage of total cellular LBPA or phospholipids, extracted, and quantified in the same experiments.
    Lbpa Standard, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lbpa standard/product/Echelon Biosciences
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lbpa standard - by Bioz Stars, 2023-02
    91/100 stars
      Buy from Supplier

    Image Search Results


    A: HeLa cells were labeled with [32P]Pi for 24 h, chased for 14 h, and then treated or not with 0.7 mM HPCD (as in Fig. 1). Lipids were extracted, separated by 2D-TLC, and visualized by autoradiography. The position of LBPA (arrow) was identified by comigration with pure synthetic LBPA as standard. Right: LBPA quantified in 2D-TLCs at different time after HPCD treatment and expressed as a percentage of total 32P-phospholipids (PL). B: Cells labeled as in A were treated or not with 0.7 mM HPCD for 1 h. The lipids were extracted from medium and separated by 2D-TLC. Right shows the quantification of LBPA and total phospholipids in medium. Data are expressed as a percentage of total cellular LBPA or phospholipids, extracted, and quantified in the same experiments.

    Journal: Journal of Lipid Research

    Article Title: Cyclodextrin triggers MCOLN1-dependent endo-lysosome secretion in Niemann-Pick type C cells

    doi: 10.1194/jlr.M089979

    Figure Lengend Snippet: A: HeLa cells were labeled with [32P]Pi for 24 h, chased for 14 h, and then treated or not with 0.7 mM HPCD (as in Fig. 1). Lipids were extracted, separated by 2D-TLC, and visualized by autoradiography. The position of LBPA (arrow) was identified by comigration with pure synthetic LBPA as standard. Right: LBPA quantified in 2D-TLCs at different time after HPCD treatment and expressed as a percentage of total 32P-phospholipids (PL). B: Cells labeled as in A were treated or not with 0.7 mM HPCD for 1 h. The lipids were extracted from medium and separated by 2D-TLC. Right shows the quantification of LBPA and total phospholipids in medium. Data are expressed as a percentage of total cellular LBPA or phospholipids, extracted, and quantified in the same experiments.

    Article Snippet: Lysobisphosphatidic acid (LBPA) was identified by comigration with 18:1/18:1 LBPA standard (Echelon Inc, Salt Lake City).

    Techniques: Labeling, Autoradiography