phosphatidylethanolamine pe beads  (Echelon Biosciences)


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    Structured Review

    Echelon Biosciences phosphatidylethanolamine pe beads
    AM strongly binds to membrane PA and partly signals via PA. ( a ) Protein-lipid overlay assay using a membrane lipid strip. The lower right spot is a negative control (Blank). TG, triglyceride; DAG, diacylglycerol; PA, phosphatidic acid; PS, phosphatidylserine; PE, <t>phosphatidylethanolamine;</t> PC, phosphatidylcholine; PG, phosphatidylglycerol; PI, phosphatidylinositol; PI(4)P, phosphatidylinositol (4)-phosphate; PI(4,5)P 2 , phosphatidylinositol (4,5)-diphosphate; PI(3,4,5)P 3 , phosphatidylinositol (3,4,5)-triphosphate; SM, sphingomyelin. ( b ) Lipid-protein pull-down assay using lipid coated beads. The beads coated either with PA, PI(4)P, or PE were incubated with recombinant murine AM protein, pelleted by centrifugation, and subjected to a western blot using anti-mouse AM antibody. ctl, beads without lipid; rmAM, recombinant murine AM protein without beads (as a positive control). ( c ) Changes in mRNA expression in AM-stimulated CFBs with PA synthesis inhibitors. *p
    Phosphatidylethanolamine Pe Beads, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphatidylethanolamine pe beads/product/Echelon Biosciences
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phosphatidylethanolamine pe beads - by Bioz Stars, 2022-05
    93/100 stars

    Images

    1) Product Images from "Hepatokine α1-Microglobulin Signaling Exacerbates Inflammation and Disturbs Fibrotic Repair in Mouse Myocardial Infarction"

    Article Title: Hepatokine α1-Microglobulin Signaling Exacerbates Inflammation and Disturbs Fibrotic Repair in Mouse Myocardial Infarction

    Journal: Scientific Reports

    doi: 10.1038/s41598-018-35194-w

    AM strongly binds to membrane PA and partly signals via PA. ( a ) Protein-lipid overlay assay using a membrane lipid strip. The lower right spot is a negative control (Blank). TG, triglyceride; DAG, diacylglycerol; PA, phosphatidic acid; PS, phosphatidylserine; PE, phosphatidylethanolamine; PC, phosphatidylcholine; PG, phosphatidylglycerol; PI, phosphatidylinositol; PI(4)P, phosphatidylinositol (4)-phosphate; PI(4,5)P 2 , phosphatidylinositol (4,5)-diphosphate; PI(3,4,5)P 3 , phosphatidylinositol (3,4,5)-triphosphate; SM, sphingomyelin. ( b ) Lipid-protein pull-down assay using lipid coated beads. The beads coated either with PA, PI(4)P, or PE were incubated with recombinant murine AM protein, pelleted by centrifugation, and subjected to a western blot using anti-mouse AM antibody. ctl, beads without lipid; rmAM, recombinant murine AM protein without beads (as a positive control). ( c ) Changes in mRNA expression in AM-stimulated CFBs with PA synthesis inhibitors. *p
    Figure Legend Snippet: AM strongly binds to membrane PA and partly signals via PA. ( a ) Protein-lipid overlay assay using a membrane lipid strip. The lower right spot is a negative control (Blank). TG, triglyceride; DAG, diacylglycerol; PA, phosphatidic acid; PS, phosphatidylserine; PE, phosphatidylethanolamine; PC, phosphatidylcholine; PG, phosphatidylglycerol; PI, phosphatidylinositol; PI(4)P, phosphatidylinositol (4)-phosphate; PI(4,5)P 2 , phosphatidylinositol (4,5)-diphosphate; PI(3,4,5)P 3 , phosphatidylinositol (3,4,5)-triphosphate; SM, sphingomyelin. ( b ) Lipid-protein pull-down assay using lipid coated beads. The beads coated either with PA, PI(4)P, or PE were incubated with recombinant murine AM protein, pelleted by centrifugation, and subjected to a western blot using anti-mouse AM antibody. ctl, beads without lipid; rmAM, recombinant murine AM protein without beads (as a positive control). ( c ) Changes in mRNA expression in AM-stimulated CFBs with PA synthesis inhibitors. *p

    Techniques Used: Protein-lipid Overlay Assay (PLOA), Stripping Membranes, Negative Control, Pull Down Assay, Incubation, Recombinant, Centrifugation, Western Blot, Positive Control, Expressing

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  • 93
    Echelon Biosciences phosphatidylethanolamine pe beads
    AM strongly binds to membrane PA and partly signals via PA. ( a ) Protein-lipid overlay assay using a membrane lipid strip. The lower right spot is a negative control (Blank). TG, triglyceride; DAG, diacylglycerol; PA, phosphatidic acid; PS, phosphatidylserine; PE, <t>phosphatidylethanolamine;</t> PC, phosphatidylcholine; PG, phosphatidylglycerol; PI, phosphatidylinositol; PI(4)P, phosphatidylinositol (4)-phosphate; PI(4,5)P 2 , phosphatidylinositol (4,5)-diphosphate; PI(3,4,5)P 3 , phosphatidylinositol (3,4,5)-triphosphate; SM, sphingomyelin. ( b ) Lipid-protein pull-down assay using lipid coated beads. The beads coated either with PA, PI(4)P, or PE were incubated with recombinant murine AM protein, pelleted by centrifugation, and subjected to a western blot using anti-mouse AM antibody. ctl, beads without lipid; rmAM, recombinant murine AM protein without beads (as a positive control). ( c ) Changes in mRNA expression in AM-stimulated CFBs with PA synthesis inhibitors. *p
    Phosphatidylethanolamine Pe Beads, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphatidylethanolamine pe beads/product/Echelon Biosciences
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phosphatidylethanolamine pe beads - by Bioz Stars, 2022-05
    93/100 stars
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    AM strongly binds to membrane PA and partly signals via PA. ( a ) Protein-lipid overlay assay using a membrane lipid strip. The lower right spot is a negative control (Blank). TG, triglyceride; DAG, diacylglycerol; PA, phosphatidic acid; PS, phosphatidylserine; PE, phosphatidylethanolamine; PC, phosphatidylcholine; PG, phosphatidylglycerol; PI, phosphatidylinositol; PI(4)P, phosphatidylinositol (4)-phosphate; PI(4,5)P 2 , phosphatidylinositol (4,5)-diphosphate; PI(3,4,5)P 3 , phosphatidylinositol (3,4,5)-triphosphate; SM, sphingomyelin. ( b ) Lipid-protein pull-down assay using lipid coated beads. The beads coated either with PA, PI(4)P, or PE were incubated with recombinant murine AM protein, pelleted by centrifugation, and subjected to a western blot using anti-mouse AM antibody. ctl, beads without lipid; rmAM, recombinant murine AM protein without beads (as a positive control). ( c ) Changes in mRNA expression in AM-stimulated CFBs with PA synthesis inhibitors. *p

    Journal: Scientific Reports

    Article Title: Hepatokine α1-Microglobulin Signaling Exacerbates Inflammation and Disturbs Fibrotic Repair in Mouse Myocardial Infarction

    doi: 10.1038/s41598-018-35194-w

    Figure Lengend Snippet: AM strongly binds to membrane PA and partly signals via PA. ( a ) Protein-lipid overlay assay using a membrane lipid strip. The lower right spot is a negative control (Blank). TG, triglyceride; DAG, diacylglycerol; PA, phosphatidic acid; PS, phosphatidylserine; PE, phosphatidylethanolamine; PC, phosphatidylcholine; PG, phosphatidylglycerol; PI, phosphatidylinositol; PI(4)P, phosphatidylinositol (4)-phosphate; PI(4,5)P 2 , phosphatidylinositol (4,5)-diphosphate; PI(3,4,5)P 3 , phosphatidylinositol (3,4,5)-triphosphate; SM, sphingomyelin. ( b ) Lipid-protein pull-down assay using lipid coated beads. The beads coated either with PA, PI(4)P, or PE were incubated with recombinant murine AM protein, pelleted by centrifugation, and subjected to a western blot using anti-mouse AM antibody. ctl, beads without lipid; rmAM, recombinant murine AM protein without beads (as a positive control). ( c ) Changes in mRNA expression in AM-stimulated CFBs with PA synthesis inhibitors. *p

    Article Snippet: Lipid-protein pull-down assayLipid-protein pull-down assay was performed with lipid coated beads (P-B000, P-B0PA, P-B004a, P-B0PE; Echelon Biosciences) as the manufacturer’s instructions.

    Techniques: Protein-lipid Overlay Assay (PLOA), Stripping Membranes, Negative Control, Pull Down Assay, Incubation, Recombinant, Centrifugation, Western Blot, Positive Control, Expressing