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tmr labeled shuttle pip carrier 2  (Echelon Biosciences)


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    Echelon Biosciences tmr labeled shuttle pip carrier 2
    Tmr Labeled Shuttle Pip Carrier 2, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tmr labeled shuttle pip carrier 2/product/Echelon Biosciences
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tmr labeled shuttle pip carrier 2 - by Bioz Stars, 2024-10
    90/100 stars

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    tmr labeled shuttle pip carrier 2  (Echelon Biosciences)
    90
    Echelon Biosciences tmr labeled shuttle pip carrier 2
    Tmr Labeled Shuttle Pip Carrier 2, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tmr labeled shuttle pip carrier 2/product/Echelon Biosciences
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tmr labeled shuttle pip carrier 2 - by Bioz Stars, 2024-10
    90/100 stars
    		  Buy from Supplier
    shuttle pip kits  (Echelon Biosciences)
    90
    Echelon Biosciences shuttle pip kits
    PI(3,5)P2 is accumulated in the mitochondria <t>in</t> <t>Ptpmt1-depleted</t> cells, and overloading of PI(3,5)P2 results in defective mitochondrial dynamics. (A) 4-OHT-treated Ptpmt1+/+/ER-Cre+ and Ptpmt1flox/flox/ER-Cre+ MEFs were transfected with Mito-DsRed2 and then immunostained with anti-PI(3,5)P2 antibody. PI(3,5)P2 was visualized using Alexa Fluor 488-conjugated anti-mouse secondary antibody. Green fluorescence intensity in Mito-DsRed2-positive areas in Ptpmt1flox/flox/ER-Cre+ cells (n = 137) was quantified and normalized against that in the Ptpmt1+/+/ER-Cre+ control (n = 131) using laser scanning cytometric analyses. Results shown are means ± standard deviations of three independent experiments. (B) PI(3,5)P2 (di-C16) (1 μM) was delivered (shuttled) into Ptpmt1+/+/ER-Cre+ MEFs using Shuttle <t>PIP</t> kits following the protocol provided by the manufacturer (Echelon Biosciences, Inc.). Carrier 2 was used to deliver PI(3,5)P2. Cells were stained with Mitotracker Red 4 h later. The percentage of the cells with fragmented mitochondria was determined. Representative results of one experiment (average of three different fields, and 200 cells were counted for each field) are shown. Experiments were repeated using two different cell clones, and similar results were obtained.
    Shuttle Pip Kits, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/shuttle pip kits/product/Echelon Biosciences
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    shuttle pip kits - by Bioz Stars, 2024-10
    90/100 stars
    		  Buy from Supplier

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    PI(3,5)P2 is accumulated in the mitochondria in Ptpmt1-depleted cells, and overloading of PI(3,5)P2 results in defective mitochondrial dynamics. (A) 4-OHT-treated Ptpmt1+/+/ER-Cre+ and Ptpmt1flox/flox/ER-Cre+ MEFs were transfected with Mito-DsRed2 and then immunostained with anti-PI(3,5)P2 antibody. PI(3,5)P2 was visualized using Alexa Fluor 488-conjugated anti-mouse secondary antibody. Green fluorescence intensity in Mito-DsRed2-positive areas in Ptpmt1flox/flox/ER-Cre+ cells (n = 137) was quantified and normalized against that in the Ptpmt1+/+/ER-Cre+ control (n = 131) using laser scanning cytometric analyses. Results shown are means ± standard deviations of three independent experiments. (B) PI(3,5)P2 (di-C16) (1 μM) was delivered (shuttled) into Ptpmt1+/+/ER-Cre+ MEFs using Shuttle PIP kits following the protocol provided by the manufacturer (Echelon Biosciences, Inc.). Carrier 2 was used to deliver PI(3,5)P2. Cells were stained with Mitotracker Red 4 h later. The percentage of the cells with fragmented mitochondria was determined. Representative results of one experiment (average of three different fields, and 200 cells were counted for each field) are shown. Experiments were repeated using two different cell clones, and similar results were obtained.

    Journal: Molecular and Cellular Biology

    Article Title: A Critical Role of Mitochondrial Phosphatase Ptpmt1 in Embryogenesis Reveals a Mitochondrial Metabolic Stress-Induced Differentiation Checkpoint in Embryonic Stem Cells

    doi: 10.1128/MCB.05629-11

    Figure Lengend Snippet: PI(3,5)P2 is accumulated in the mitochondria in Ptpmt1-depleted cells, and overloading of PI(3,5)P2 results in defective mitochondrial dynamics. (A) 4-OHT-treated Ptpmt1+/+/ER-Cre+ and Ptpmt1flox/flox/ER-Cre+ MEFs were transfected with Mito-DsRed2 and then immunostained with anti-PI(3,5)P2 antibody. PI(3,5)P2 was visualized using Alexa Fluor 488-conjugated anti-mouse secondary antibody. Green fluorescence intensity in Mito-DsRed2-positive areas in Ptpmt1flox/flox/ER-Cre+ cells (n = 137) was quantified and normalized against that in the Ptpmt1+/+/ER-Cre+ control (n = 131) using laser scanning cytometric analyses. Results shown are means ± standard deviations of three independent experiments. (B) PI(3,5)P2 (di-C16) (1 μM) was delivered (shuttled) into Ptpmt1+/+/ER-Cre+ MEFs using Shuttle PIP kits following the protocol provided by the manufacturer (Echelon Biosciences, Inc.). Carrier 2 was used to deliver PI(3,5)P2. Cells were stained with Mitotracker Red 4 h later. The percentage of the cells with fragmented mitochondria was determined. Representative results of one experiment (average of three different fields, and 200 cells were counted for each field) are shown. Experiments were repeated using two different cell clones, and similar results were obtained.

    Article Snippet: Results shown are means ± standard deviations of three independent experiments. (B) PI(3,5)P 2 (di-C 16 ) (1 μM) was delivered (shuttled) into Ptpmt1 +/+ / ER-Cre + MEFs using Shuttle PIP kits following the protocol provided by the manufacturer (Echelon Biosciences, Inc.).

    Techniques: Transfection, Fluorescence, Staining, Clone Assay