p ampk thr 172  (Cell Signaling Technology Inc)


Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc p ampk thr 172
    P Ampk Thr 172, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    p thr 172 ampk  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc p thr 172 ampk
    P Thr 172 Ampk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    p ampk thr 172  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc p ampk thr 172
    List of primary antibodies used for immunoblotting.
    P Ampk Thr 172, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Insulin, dibutyryl-cAMP, and glucose modulate expression of patatin-like domain containing protein 7 in cultured human myotubes"

    Article Title: Insulin, dibutyryl-cAMP, and glucose modulate expression of patatin-like domain containing protein 7 in cultured human myotubes

    Journal: Frontiers in Endocrinology

    doi: 10.3389/fendo.2023.1139303

    List of primary antibodies used for immunoblotting.
    Figure Legend Snippet: List of primary antibodies used for immunoblotting.

    Techniques Used: Western Blot

    thr 172 p ampk rrid ab 2169396  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc thr 172 p ampk rrid ab 2169396
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    p 172 thr adenosine monophosphate protein kinase ampk  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc p 172 thr adenosine monophosphate protein kinase ampk
    P 172 Thr Adenosine Monophosphate Protein Kinase Ampk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    p ampk thr 172  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc p ampk thr 172
    AMPK activation is not required for metformin inhibition of prostate cancer cells. Prostate cancer cells were treated with increasing concentrations of AICAR, an AMPK activator, Compound C, an AMPK inhibitor or a combination of AMPK inhibitor and metformin, respectively. LNCaP cells were treated with the AMPK activator AICAR. After 96 h AR levels were quantified by western blot and normalized to GAPDH. Activation of AMPK was verified by detection of <t>phosphorylated</t> <t>AMPK</t> by western blot <t>(P-AMPK/AMPK</t> ratio) (A) . Prostate cancer cells were treated with increasing concentrations of the AMPK inhibitor compound-C (0–10 μM) and cell numbers (B) and AR protein levels (C) in LNCaP and DuCaP cells were determined after 96 h. Inhibition of AMPK was verified by detection of phosporylated AMPK (P-AMPK/AMPK ratio) (C) ). Representative fluoroscan images of LNCaP and DuCaP cell western blots are shown in (D) . Addition of 1 mM of metformin to the AMPK inhibitor compound-C resulted in enhanced inhibition of cell proliferation in LNCaP and PC3 cells (E) and further reduction of AR protein levels in LNCaP cells (F) . AR levels and P-AMPK/AMPK ratios in Figure were quantified by western blot densitometry. The histograms show the data of at least three independent experiments, the fluoroscan image shows representative western blots. Statistical significant differences are indicated as *, p < 0.05; **, p <0.01 and ***, p < 0.001.
    P Ampk Thr 172, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Metformin anti-tumor effect via disruption of the MID1 translational regulator complex and AR downregulation in prostate cancer cells"

    Article Title: Metformin anti-tumor effect via disruption of the MID1 translational regulator complex and AR downregulation in prostate cancer cells

    Journal: BMC Cancer

    doi: 10.1186/1471-2407-14-52

    AMPK activation is not required for metformin inhibition of prostate cancer cells. Prostate cancer cells were treated with increasing concentrations of AICAR, an AMPK activator, Compound C, an AMPK inhibitor or a combination of AMPK inhibitor and metformin, respectively. LNCaP cells were treated with the AMPK activator AICAR. After 96 h AR levels were quantified by western blot and normalized to GAPDH. Activation of AMPK was verified by detection of phosphorylated AMPK by western blot (P-AMPK/AMPK ratio) (A) . Prostate cancer cells were treated with increasing concentrations of the AMPK inhibitor compound-C (0–10 μM) and cell numbers (B) and AR protein levels (C) in LNCaP and DuCaP cells were determined after 96 h. Inhibition of AMPK was verified by detection of phosporylated AMPK (P-AMPK/AMPK ratio) (C) ). Representative fluoroscan images of LNCaP and DuCaP cell western blots are shown in (D) . Addition of 1 mM of metformin to the AMPK inhibitor compound-C resulted in enhanced inhibition of cell proliferation in LNCaP and PC3 cells (E) and further reduction of AR protein levels in LNCaP cells (F) . AR levels and P-AMPK/AMPK ratios in Figure were quantified by western blot densitometry. The histograms show the data of at least three independent experiments, the fluoroscan image shows representative western blots. Statistical significant differences are indicated as *, p < 0.05; **, p <0.01 and ***, p < 0.001.
    Figure Legend Snippet: AMPK activation is not required for metformin inhibition of prostate cancer cells. Prostate cancer cells were treated with increasing concentrations of AICAR, an AMPK activator, Compound C, an AMPK inhibitor or a combination of AMPK inhibitor and metformin, respectively. LNCaP cells were treated with the AMPK activator AICAR. After 96 h AR levels were quantified by western blot and normalized to GAPDH. Activation of AMPK was verified by detection of phosphorylated AMPK by western blot (P-AMPK/AMPK ratio) (A) . Prostate cancer cells were treated with increasing concentrations of the AMPK inhibitor compound-C (0–10 μM) and cell numbers (B) and AR protein levels (C) in LNCaP and DuCaP cells were determined after 96 h. Inhibition of AMPK was verified by detection of phosporylated AMPK (P-AMPK/AMPK ratio) (C) ). Representative fluoroscan images of LNCaP and DuCaP cell western blots are shown in (D) . Addition of 1 mM of metformin to the AMPK inhibitor compound-C resulted in enhanced inhibition of cell proliferation in LNCaP and PC3 cells (E) and further reduction of AR protein levels in LNCaP cells (F) . AR levels and P-AMPK/AMPK ratios in Figure were quantified by western blot densitometry. The histograms show the data of at least three independent experiments, the fluoroscan image shows representative western blots. Statistical significant differences are indicated as *, p < 0.05; **, p <0.01 and ***, p < 0.001.

    Techniques Used: Activation Assay, Inhibition, Western Blot

    p ampk thr 172  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc p ampk thr 172
    Protein-protein interactions and phosphorylation of signaling proteins in longissimus dorsi muscle of neonatal pigs
    P Ampk Thr 172, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Differential regulation of mTORC1 activation by leucine and β-hydroxy-β-methylbutyrate in skeletal muscle of neonatal pigs"

    Article Title: Differential regulation of mTORC1 activation by leucine and β-hydroxy-β-methylbutyrate in skeletal muscle of neonatal pigs

    Journal: Journal of Applied Physiology

    doi: 10.1152/japplphysiol.00332.2019

    Protein-protein interactions and phosphorylation of signaling proteins in longissimus dorsi muscle of neonatal pigs
    Figure Legend Snippet: Protein-protein interactions and phosphorylation of signaling proteins in longissimus dorsi muscle of neonatal pigs

    Techniques Used:

    p ampk thr 172  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc p ampk thr 172
    Protein-protein interactions and phosphorylation of signaling proteins in longissimus dorsi muscle of neonatal pigs
    P Ampk Thr 172, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p ampk thr 172/product/Cell Signaling Technology Inc
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    1) Product Images from "Differential regulation of mTORC1 activation by leucine and β-hydroxy-β-methylbutyrate in skeletal muscle of neonatal pigs"

    Article Title: Differential regulation of mTORC1 activation by leucine and β-hydroxy-β-methylbutyrate in skeletal muscle of neonatal pigs

    Journal: Journal of Applied Physiology

    doi: 10.1152/japplphysiol.00332.2019

    Protein-protein interactions and phosphorylation of signaling proteins in longissimus dorsi muscle of neonatal pigs
    Figure Legend Snippet: Protein-protein interactions and phosphorylation of signaling proteins in longissimus dorsi muscle of neonatal pigs

    Techniques Used:

    rabbit anti p ampk thr 172  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc rabbit anti p ampk thr 172
    Rabbit Anti P Ampk Thr 172, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    p ampk thr 172  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc p ampk thr 172
    P Ampk Thr 172, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p ampk thr 172/product/Cell Signaling Technology Inc
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