Journal: Journal of Thoracic Disease

Article Title: Bufalin inhibits epithelial-mesenchymal transition and increases radiosensitivity of non-small cell lung cancer via inhibition of the Src signaling

doi: 10.21037/jtd-22-1859

Figure Lengend Snippet: Bufalin inhibits EMT and abrogates radiation-induced EMT. (A) Western blot analysis for protein levels of E-cadherin, N-cadherin, vimentin, Slug, and Snail after treatment of A549 cells with increasing concentrations of bufalin. (B) Western blots to assess protein levels of E-cadherin, N-cadherin, vimentin, Slug, and Snail after treatment of A549 cells with DMSO, bufalin, radiation, and combined treatment (50 nM bufalin + radiation 4 Gy). (C) Cells were treated with radiation (4 Gy) or radiation plus bufalin; the changes of vimentin and E-cadherin in A549 cells were visualized by immunofluorescence microscopy. Scale bar, 20 µm. (D) Western blot analysis for protein levels of p-Y416 Src, Src (32G6), p-STAT3, and STAT3 after treatment of A549 cells with increasing concentrations of bufalin. (E) Representative immunofluorescence micrographs of A549 cells stained with antibodies against p-Y416 Src and p-STAT3. Scale bar, 20 µm. Ctrl, control; EMT, epithelial-mesenchymal transition; DMSO, dimethyl sulfoxide.

Article Snippet: The antibodies used in this study were purchased from Cell Signaling Technology (CST; Danvers, MA, USA), including anti-Src (#2123), anti-p-Y416-Src (#2102), anti-STAT3 (#12640), anti-p-STAT3 (#9145), anti-E-cadherin (#3195), anti-snail (#3879), anti-snail2 (#9585), anti-vimentin (#5741), anti-N-cadherin (#13116), anti-Vinculin (#4650), anti-GAPDH (#5174), and anti-β-actin (#3700).

Techniques: Western Blot, Immunofluorescence, Microscopy, Staining

Journal: PLoS ONE

Article Title: Areca Nut Components Affect COX-2, Cyclin B1/cdc25C and Keratin Expression, PGE2 Production in Keratinocyte Is Related to Reactive Oxygen Species, CYP1A1, Src, EGFR and Ras Signaling

doi: 10.1371/journal.pone.0101959

Figure Lengend Snippet: ( A ) AN extract (ANE) stimulated EGFR (Tyr845) phorphorylation within 60 min of exposure as analyzed by Pathscan p-EGFR ELISA. *denotes significant difference when compared with solvent control. (n = 10). ( B ) Pretreatment by PD153035 (1 and 5 µM, an EGFR antagonist) markedly attenuated the ANE-induced PGE 2 production in GK. ( C ) Effect of PD153035 on ANE-induced COX-2 expression in GK. One representative PCR result was shown. *denotes significant difference when compared with solvent control. #denotes significant difference when compared with ANE (800 µg/ml)-treated group.

Article Snippet: Pathscan p-Src (Y416) and p-EGFR (Tyr845) ELISA kits were from Cell Signaling (Cell Signaling Technology Inc., MA, USA).

Techniques: Enzyme-linked Immunosorbent Assay, Expressing

Journal: PLoS ONE

Article Title: Areca Nut Components Affect COX-2, Cyclin B1/cdc25C and Keratin Expression, PGE2 Production in Keratinocyte Is Related to Reactive Oxygen Species, CYP1A1, Src, EGFR and Ras Signaling

doi: 10.1371/journal.pone.0101959

Figure Lengend Snippet: ( A ) GK were exposed to ANE (800 µg/ml) for different time points. Cell lysates were collected and used for analysis of Src phosphorylation (p-Src) by pathscan p-ELISA. Results were expressed as absorbance (OD450) (n = 9). *denotes significant difference when compared with control. ( B ) Inhibition of ANE-induced Src phosphorylation in GK by pp2, ( C ) pp2 inhibited the ANE-induced COX-2 mRNA and protein expression in GK. One representative PCR and western blotting result was shown. ( D ) pp2 prevented the ANE-induced PGE 2 production in GK (n = 5). ( E ) ANE induced the activation of Ras in GK. GK were exposed to ANE (800 µg/ml) for different time points (30–150 secs). Cell lysates were collected and used for analysis of Ras activation using Ras activation assay kit (Pierce). GTPγS-activated was the positive control. ( F ) Manumycin A (1 and 5 µM) effectively prevented the ANE-induced COX-2 expression of GK, ( G ) Manumycin A also attenuated the ANE-induced PGE2 production of GK. *denotes significant difference when compared with solvent control. #denotes statistical significant difference when compared with ANE (800 µg/ml) solely-treated group (P<0.05)

Article Snippet: Pathscan p-Src (Y416) and p-EGFR (Tyr845) ELISA kits were from Cell Signaling (Cell Signaling Technology Inc., MA, USA).

Techniques: Enzyme-linked Immunosorbent Assay, Inhibition, Expressing, Western Blot, Activation Assay, Positive Control