Journal: Neural Regeneration Research

Article Title: Photobiomodulation inhibits the expression of chondroitin sulfate proteoglycans after spinal cord injury via the Sox9 pathway

doi: 10.4103/1673-5374.374136

Figure Lengend Snippet: PBM modulates the Smad3/Sox9 and MAPK/Sox9 pathways in inflammation-induced astrocytes. (A, B) Representative western blots of proteins related to the Smad3/Sox9 pathway, Erk/Sox9 pathway, P38/Sox9 pathway, and JNK/Sox9 pathway in astrocytes and quantification of the relative expression levels of p-Smad3, p-Erk, p-P38, p-JNK, Sox9, and versican. Data are shown as mean ± SD ( n = 3 for each group) and were analyzed by multiple repeated measures analysis of variance with least significance difference post hoc analysis. * P < 0.05, *** P < 0.001, **** P < 0.0001. Erk: Extracellular signal-regulated kinase; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; JNK: c-Jun N-terminal kinase.

Article Snippet: The membranes were soaked in 5% skimmed milk for 1 hour at room temperature and incubated overnight at 4°C with the following primary antibodies: anti-mouse glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:3000, Proteintech, Wuhan, Hubei, China, Cat# 60004-1-Ig, RRID: AB_2107436), anti-mouse chondroitin sulfate proteoglycans (CSPG) (1:1000, Sigma-Aldrich, St. Louis, MO, USA, Cat# C8035, RRID: AB_476879), anti-mouse versican (1:1000, Thermo Fisher Scientific, Cat# S351-23, RRID: AB_2735409), anti-rabbit Sox9 (1:1000, Abcam, Cambridge, UK, Cat# ab185966, RRID: AB_2728660), anti-rabbit p-P38 MAPK (P38) (1:1000, CST, Danvers, MA, USA, Cat# D3F9, RRID: AB_2139682), anti-rabbit P38 (1:1000, CST, Cat# D13E1, RRID: TSC_SD02390), anti-rabbit p-extracellular signal-regulated kinase 1/2 (Erk1/2) (1:1000, CST, Cat# D13.14.4E, RRID: AB_10694057), anti-rabbit Erk1/2 (1:1000, CST, Cat# 137F5, RRID: AB_10693607), anti-rabbit p-Smad3 (1:1000, CST, Cat# C25A9, RRID: AB_2193207), anti-rabbit Smad2/3 (1:1000, CST, Cat# D7G7, RRID: AB_10889933), anti-rabbit p-c-Jun N-terminal kinase (JNK) (1:1000, Proteintech, Cat# 80024-1-RR, RRID: AB_2882943), and anti-rabbit JNK (1:1000, Proteintech, Cat# 66210-1-Ig, RRID: AB_2881601).

Techniques: Western Blot, Expressing

Journal: Neural Regeneration Research

Article Title: Photobiomodulation inhibits the expression of chondroitin sulfate proteoglycans after spinal cord injury via the Sox9 pathway

doi: 10.4103/1673-5374.374136

Figure Lengend Snippet: The Smad3/Sox9 pathway and MAPK/Sox9 pathway are involved in photobiomodulation (PBM) modulation of versican expression in spinal cord injury (SCI) mice. (A–C) Representative western blots of proteins related to the Erk/Sox9 pathway (A), P38/Sox9 pathway (B), and Smad3/Sox9 pathway (C) at 7 dpi and quantification of the levels of p-Smad3, p-Erk, p-P38, Sox9, and versican ( n = 6 mice for each group). Data are shown as mean ± SD and were analyzed by one-way analysis of variance with least significance difference post hoc analysis. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. dpi: Day(s) post-injury; Erk: extracellular signal-regulated kinase; GAPDH: glyceraldehyde-3-phosphate dehydrogenase.

Article Snippet: The membranes were soaked in 5% skimmed milk for 1 hour at room temperature and incubated overnight at 4°C with the following primary antibodies: anti-mouse glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:3000, Proteintech, Wuhan, Hubei, China, Cat# 60004-1-Ig, RRID: AB_2107436), anti-mouse chondroitin sulfate proteoglycans (CSPG) (1:1000, Sigma-Aldrich, St. Louis, MO, USA, Cat# C8035, RRID: AB_476879), anti-mouse versican (1:1000, Thermo Fisher Scientific, Cat# S351-23, RRID: AB_2735409), anti-rabbit Sox9 (1:1000, Abcam, Cambridge, UK, Cat# ab185966, RRID: AB_2728660), anti-rabbit p-P38 MAPK (P38) (1:1000, CST, Danvers, MA, USA, Cat# D3F9, RRID: AB_2139682), anti-rabbit P38 (1:1000, CST, Cat# D13E1, RRID: TSC_SD02390), anti-rabbit p-extracellular signal-regulated kinase 1/2 (Erk1/2) (1:1000, CST, Cat# D13.14.4E, RRID: AB_10694057), anti-rabbit Erk1/2 (1:1000, CST, Cat# 137F5, RRID: AB_10693607), anti-rabbit p-Smad3 (1:1000, CST, Cat# C25A9, RRID: AB_2193207), anti-rabbit Smad2/3 (1:1000, CST, Cat# D7G7, RRID: AB_10889933), anti-rabbit p-c-Jun N-terminal kinase (JNK) (1:1000, Proteintech, Cat# 80024-1-RR, RRID: AB_2882943), and anti-rabbit JNK (1:1000, Proteintech, Cat# 66210-1-Ig, RRID: AB_2881601).

Techniques: Expressing, Western Blot

Journal: International Journal of Molecular Medicine

Article Title: Exosomes derived from human dermal fibroblasts protect against UVB-induced skin photoaging

doi: 10.3892/ijmm.2023.5323

Figure Lengend Snippet: Antibodies used for western blot analysis.

Article Snippet: Anti-p-p38 (Thr 180 /Tyr 182 ) , 4511s , Cell Signaling Technology, Inc. , 1:1,000.

Techniques: Western Blot

Journal: Oncology Reports

Article Title: Licochalcone A induces cell cycle arrest and apoptosis via suppressing MAPK signaling pathway and the expression of FBXO5 in lung squamous cell cancer

doi: 10.3892/or.2023.8651

Figure Lengend Snippet: Network pharmacology analysis of the therapeutic target of LCA against LSCC. (A) Venn diagram revealed 17 overlapping genes for LCA target genes and LSCC-related genes. (B) The bubble chart illustrated enriched Kyoto Encyclopedia of Genes and Genomes pathways related to the therapeutic target. (C) Protein-protein interaction analysis revealed interaction of the 17 overlapping genes. (D-F) Western blot analysis results revealed the protein expression of p38 MAPK, p-p38 MAPK, ERK1/2 and p-ERK1/2 after treatment with LCA (0, 10, 20 and 40 µM) in LSCC cells. The experiments were repeated at least three times (n=3). **P<0.01 vs. control. LCA, licochalcone A; LSCC, lung squamous cell carcinoma; p-, phosphorylated.

Article Snippet: CDK2 (1:1,000; cat. no. ab32147; Abcam), CDK4 (1:1,000; cat. no. ab108357; Abcam), cyclin D1 (1:5,000; cat. no. ab134175; Abcam), cyclin E (1:1,000; cat. no. WL01072; Wanleibio Co., Ltd.), cleaved caspase 3 (1:1,000; cat. no. AF7022; Affinity Biosciences, Ltd.), cleaved poly(ADP-ribose)polymerase-1 (PARP1) (1:5,000; cat. no. ab32064; Abcam), ERK (1:1,000; cat. no. ab184699; Abcam), phosphorylated (p-)ERK (1:1,000; cat. no. ab201015; Abcam), p38 MAPK (1:1,000; cat. no. 8690; Cell Signaling Technology, Inc.), p-p38 MAPK (1:1,000; cat. no. 4511; Cell Signaling Technology, Inc.), FBXO5 (1:5,000; cat. no. ab184950; Abcam), GAPDH (1:5,000; cat. no. 60004-1-IG; Proteintech Group, Inc.), HRP-goat anti-mouse (1:5,000; cat. no. EM35110-01; Beijing Emarbio Science & Technology Co., Ltd.) and HRP-goat anti-rabbit antibody (1:5,000; cat. no. EM35111-01; Beijing Emarbio Science & Technology Co., Ltd.).

Techniques: Western Blot, Expressing

Journal: Oncology Reports

Article Title: Licochalcone A induces cell cycle arrest and apoptosis via suppressing MAPK signaling pathway and the expression of FBXO5 in lung squamous cell cancer

doi: 10.3892/or.2023.8651

Figure Lengend Snippet: LCA suppresses lung squamous cell carcinoma xenograft tumor growth in vivo (n=5). (A) Representative images of xenograft tumor models and tumor tissues. (B) The wet weight of the mice xenograft tumors. (C) The tumor volumes of the mice xenograft tumors. (D) The body weight of the xenograft tumor mice. (E) The organ weight of the xenograft tumor mice. (F) The hematoxylin and eosin staining representative pictures of vital organs of the xenograft tumors mice (scale bar, 200 µm). (G and H) The levels of FBXO5, p-p38 MAPK and p-ERK1/2 in tumor tissue determined by western blot analysis. The experiments were repeated at least three times (n=3). *P<0.05 and **P<0.01 vs. control. LCA, licochalcone A; FBXO5, F-box protein 5; CDDP, cisplatin; p-, phosphorylated. The body weight of the xenograft tumor mice.

Article Snippet: CDK2 (1:1,000; cat. no. ab32147; Abcam), CDK4 (1:1,000; cat. no. ab108357; Abcam), cyclin D1 (1:5,000; cat. no. ab134175; Abcam), cyclin E (1:1,000; cat. no. WL01072; Wanleibio Co., Ltd.), cleaved caspase 3 (1:1,000; cat. no. AF7022; Affinity Biosciences, Ltd.), cleaved poly(ADP-ribose)polymerase-1 (PARP1) (1:5,000; cat. no. ab32064; Abcam), ERK (1:1,000; cat. no. ab184699; Abcam), phosphorylated (p-)ERK (1:1,000; cat. no. ab201015; Abcam), p38 MAPK (1:1,000; cat. no. 8690; Cell Signaling Technology, Inc.), p-p38 MAPK (1:1,000; cat. no. 4511; Cell Signaling Technology, Inc.), FBXO5 (1:5,000; cat. no. ab184950; Abcam), GAPDH (1:5,000; cat. no. 60004-1-IG; Proteintech Group, Inc.), HRP-goat anti-mouse (1:5,000; cat. no. EM35110-01; Beijing Emarbio Science & Technology Co., Ltd.) and HRP-goat anti-rabbit antibody (1:5,000; cat. no. EM35111-01; Beijing Emarbio Science & Technology Co., Ltd.).

Techniques: In Vivo, Staining, Western Blot