p falciparum  (Roche)


Bioz Verified Symbol Roche is a verified supplier
Bioz Manufacturer Symbol Roche manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Roche p falciparum
    P Falciparum, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p falciparum/product/Roche
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    p falciparum - by Bioz Stars, 2021-05
    86/100 stars

    Images

    Related Articles

    In Vitro:

    Article Title: Probing the Antimalarial Mechanism of Artemisinin and OZ277 (Arterolane) with Nonperoxidic Isosteres and Nitroxyl Radicals ▿
    Article Snippet: .. The chloroquine-sensitive NF54 isolate and the chloroquine- and pyrimethamine-resistant K1 isolate of P. falciparum , provided by F. Hoffmann-La Roche Ltd. (Basel, Switzerland), were used for the in vitro assays. ..

    Article Title: Pharmacokinetic-Pharmacodynamic Analysis of Spiroindolone Analogs and KAE609 in a Murine Malaria Model
    Article Snippet: .. In vitro antimalarial activity was measured using the [3 H]hypoxanthine incorporation assay ( ) with strain NF54 of P. falciparum (obtained from Hoffmann-LaRoche Ltd.). .. The compounds were dissolved in dimethyl sulfoxide (DMSO) at a concentration of 10 mM, diluted in hypoxanthine-free culture medium, and titrated in duplicate over a 64-fold range in 96-well plates.

    Expressing:

    Article Title: Selective inhibition of apicoplast tryptophanyl-tRNA synthetase causes delayed death in Plasmodium falciparum
    Article Snippet: .. Immunoblotting To verify the expression of TrpRSapi _pGlux and TrpRScyt _pGlux in P. falciparum , erythrocytes infected with trophozoite stage parasites (8–10% parasitemia) were lysed with 0.01% saponin and complete™, EDTA-free Protease Inhibitor (Roche) resuspended in PBS (1x) for 10 minutes at 4 °C. ..

    Article Title: A new double-antibody sandwich ELISA targeting Plasmodium falciparum aldolase to evaluate anti-malarial drug sensitivity
    Article Snippet: .. The recombinant P. falciparum aldolases P41/5 (S359T) and P41/7 (K365N) were obtained from lambdaGT11 phage expression libraries constructed with genomic DNA of the K1 isolate of P. falciparum and were kindly provided by H. Döbeli, (F. Hoffmann-La Roche Ltd, Basel, Switzerland) [ , ]. .. Generation of hybridoma cell lines producing anti-Plasmodium falciparum aldolase antibodies Anti-P. falciparum aldolase antibodies were identified by immunoprecipitation with Protein A-sepharose (GE Healthcare) in the soluble fraction of an infected culture.

    Article Title: An Inhibitory Antibody Blocks Interactions between Components of the Malarial Invasion Machinery
    Article Snippet: To introduce the tag, sequence encoding 459KRIKLNDND467 in construct pST2A-sgPfa1 was modified to encode the HA tag by Quikchange site-directed mutagenesis (Stratagene), resulting in construct pST2A-sgA1/DIII-HA. .. To produce a construct for episomal expression of the full-length HA-tagged protein (PfAMA1-DIII-HA) in P. falciparum under control of the authentic ama1 promoter, sequence spanning the HA tag was excised using Stu I and Age I (Roche) and cloned into pHAM-sgPfa1/HA restricted with the same enzymes, giving rise to plasmid pHAM-sgA1/DIII-HA. .. For expression of various mutants of PfAMA1-DIII-HA, appropriate mutations were introduced into pST2A-sgPfa1 by Quikchange site-directed mutagenesis, and sequence encompassing these mutations sub-cloned by restriction with Pst I and Nco I into pHAM-sgA1/DIII-HA.

    Infection:

    Article Title: Selective inhibition of apicoplast tryptophanyl-tRNA synthetase causes delayed death in Plasmodium falciparum
    Article Snippet: .. Immunoblotting To verify the expression of TrpRSapi _pGlux and TrpRScyt _pGlux in P. falciparum , erythrocytes infected with trophozoite stage parasites (8–10% parasitemia) were lysed with 0.01% saponin and complete™, EDTA-free Protease Inhibitor (Roche) resuspended in PBS (1x) for 10 minutes at 4 °C. ..

    Article Title: Baseline Ex Vivo and Molecular Responses of Plasmodium falciparum Isolates to Piperaquine before Implementation of Dihydroartemisinin-Piperaquine in Senegal
    Article Snippet: Thin blood smears were stained using a RAL kit (Réactifs RAL, Paris, France) based on eosin and methylene blue and were examined to determine the infection by P. falciparum and parasitemia. .. The diagnosis of monospecies infection by P. falciparum was confirmed by real-time PCR (LightCycler 2.0; Roche Group, Basel, Switzerland), as previously described ( ). ..

    Protease Inhibitor:

    Article Title: Selective inhibition of apicoplast tryptophanyl-tRNA synthetase causes delayed death in Plasmodium falciparum
    Article Snippet: .. Immunoblotting To verify the expression of TrpRSapi _pGlux and TrpRScyt _pGlux in P. falciparum , erythrocytes infected with trophozoite stage parasites (8–10% parasitemia) were lysed with 0.01% saponin and complete™, EDTA-free Protease Inhibitor (Roche) resuspended in PBS (1x) for 10 minutes at 4 °C. ..

    Software:

    Article Title: Evaluation of FRET real-time PCR assay for rapid detection and differentiation of Plasmodium species in returning travellers and migrants
    Article Snippet: According to these definitions, we found that the melt curve means Tms (standard deviation) in clinical isolates were 60.5°C (0.6°C) for P. falciparum DNA amplicons and 64.6°C (1.8°C) for non-P. falciparum sp DNA amplicons. .. These results were closely related to melting temperature of 60.0 and 65.0°C for P. falciparum and non-P. falciparum sp respectively, that were estimated during primer and probe design using Oligo 4.0 software (LightCycler Probe Design Software, Roche). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Pfhrp2 and pfhrp3 polymorphisms in Plasmodium falciparum isolates from Dakar, Senegal: impact on rapid malaria diagnostic tests
    Article Snippet: .. To confirm the diagnosis obtained by the RDTs and the blood smear examination, P. falciparum and P. vivax detection was performed by real-time PCR using a LightCycler® (Roche, Meylan, France). .. The following oligonucleotide primers and probes, designed with Primer Express software v2.0 (Applied Biosystems), were used: forward 5’-TTTATGTATTGGTATAATTCGG-3’, reverse-5’- GGCAAATAACTTTATCATAGAATTGAC-3’ and probe-5’-FAM-TACACTACCAACACATGGGGCTACAAGAGGT-BBQ-3’ for the P. falciparum aquaglyceroporin gene (AJ413249); forward-5’-GTGGCCGCCTTTTTGCT-3’, reverse-5’-CCTCCCTGAAACAAGTCATCG-3’ and probe-5’-HEX-CATCTACGTGGACAACGGGCTCAACA-BHQ1-3’ for the P. vivax enoyl-acyl carrier protein reductase gene (AY423076) (Eurogentec, Angers, France).

    Article Title: Baseline Ex Vivo and Molecular Responses of Plasmodium falciparum Isolates to Piperaquine before Implementation of Dihydroartemisinin-Piperaquine in Senegal
    Article Snippet: Thin blood smears were stained using a RAL kit (Réactifs RAL, Paris, France) based on eosin and methylene blue and were examined to determine the infection by P. falciparum and parasitemia. .. The diagnosis of monospecies infection by P. falciparum was confirmed by real-time PCR (LightCycler 2.0; Roche Group, Basel, Switzerland), as previously described ( ). ..

    Recombinant:

    Article Title: A new double-antibody sandwich ELISA targeting Plasmodium falciparum aldolase to evaluate anti-malarial drug sensitivity
    Article Snippet: .. The recombinant P. falciparum aldolases P41/5 (S359T) and P41/7 (K365N) were obtained from lambdaGT11 phage expression libraries constructed with genomic DNA of the K1 isolate of P. falciparum and were kindly provided by H. Döbeli, (F. Hoffmann-La Roche Ltd, Basel, Switzerland) [ , ]. .. Generation of hybridoma cell lines producing anti-Plasmodium falciparum aldolase antibodies Anti-P. falciparum aldolase antibodies were identified by immunoprecipitation with Protein A-sepharose (GE Healthcare) in the soluble fraction of an infected culture.

    Construct:

    Article Title: A new double-antibody sandwich ELISA targeting Plasmodium falciparum aldolase to evaluate anti-malarial drug sensitivity
    Article Snippet: .. The recombinant P. falciparum aldolases P41/5 (S359T) and P41/7 (K365N) were obtained from lambdaGT11 phage expression libraries constructed with genomic DNA of the K1 isolate of P. falciparum and were kindly provided by H. Döbeli, (F. Hoffmann-La Roche Ltd, Basel, Switzerland) [ , ]. .. Generation of hybridoma cell lines producing anti-Plasmodium falciparum aldolase antibodies Anti-P. falciparum aldolase antibodies were identified by immunoprecipitation with Protein A-sepharose (GE Healthcare) in the soluble fraction of an infected culture.

    Article Title: An Inhibitory Antibody Blocks Interactions between Components of the Malarial Invasion Machinery
    Article Snippet: To introduce the tag, sequence encoding 459KRIKLNDND467 in construct pST2A-sgPfa1 was modified to encode the HA tag by Quikchange site-directed mutagenesis (Stratagene), resulting in construct pST2A-sgA1/DIII-HA. .. To produce a construct for episomal expression of the full-length HA-tagged protein (PfAMA1-DIII-HA) in P. falciparum under control of the authentic ama1 promoter, sequence spanning the HA tag was excised using Stu I and Age I (Roche) and cloned into pHAM-sgPfa1/HA restricted with the same enzymes, giving rise to plasmid pHAM-sgA1/DIII-HA. .. For expression of various mutants of PfAMA1-DIII-HA, appropriate mutations were introduced into pST2A-sgPfa1 by Quikchange site-directed mutagenesis, and sequence encompassing these mutations sub-cloned by restriction with Pst I and Nco I into pHAM-sgA1/DIII-HA.

    Sequencing:

    Article Title: An Inhibitory Antibody Blocks Interactions between Components of the Malarial Invasion Machinery
    Article Snippet: To introduce the tag, sequence encoding 459KRIKLNDND467 in construct pST2A-sgPfa1 was modified to encode the HA tag by Quikchange site-directed mutagenesis (Stratagene), resulting in construct pST2A-sgA1/DIII-HA. .. To produce a construct for episomal expression of the full-length HA-tagged protein (PfAMA1-DIII-HA) in P. falciparum under control of the authentic ama1 promoter, sequence spanning the HA tag was excised using Stu I and Age I (Roche) and cloned into pHAM-sgPfa1/HA restricted with the same enzymes, giving rise to plasmid pHAM-sgA1/DIII-HA. .. For expression of various mutants of PfAMA1-DIII-HA, appropriate mutations were introduced into pST2A-sgPfa1 by Quikchange site-directed mutagenesis, and sequence encompassing these mutations sub-cloned by restriction with Pst I and Nco I into pHAM-sgA1/DIII-HA.

    Clone Assay:

    Article Title: An Inhibitory Antibody Blocks Interactions between Components of the Malarial Invasion Machinery
    Article Snippet: To introduce the tag, sequence encoding 459KRIKLNDND467 in construct pST2A-sgPfa1 was modified to encode the HA tag by Quikchange site-directed mutagenesis (Stratagene), resulting in construct pST2A-sgA1/DIII-HA. .. To produce a construct for episomal expression of the full-length HA-tagged protein (PfAMA1-DIII-HA) in P. falciparum under control of the authentic ama1 promoter, sequence spanning the HA tag was excised using Stu I and Age I (Roche) and cloned into pHAM-sgPfa1/HA restricted with the same enzymes, giving rise to plasmid pHAM-sgA1/DIII-HA. .. For expression of various mutants of PfAMA1-DIII-HA, appropriate mutations were introduced into pST2A-sgPfa1 by Quikchange site-directed mutagenesis, and sequence encompassing these mutations sub-cloned by restriction with Pst I and Nco I into pHAM-sgA1/DIII-HA.

    Plasmid Preparation:

    Article Title: An Inhibitory Antibody Blocks Interactions between Components of the Malarial Invasion Machinery
    Article Snippet: To introduce the tag, sequence encoding 459KRIKLNDND467 in construct pST2A-sgPfa1 was modified to encode the HA tag by Quikchange site-directed mutagenesis (Stratagene), resulting in construct pST2A-sgA1/DIII-HA. .. To produce a construct for episomal expression of the full-length HA-tagged protein (PfAMA1-DIII-HA) in P. falciparum under control of the authentic ama1 promoter, sequence spanning the HA tag was excised using Stu I and Age I (Roche) and cloned into pHAM-sgPfa1/HA restricted with the same enzymes, giving rise to plasmid pHAM-sgA1/DIII-HA. .. For expression of various mutants of PfAMA1-DIII-HA, appropriate mutations were introduced into pST2A-sgPfa1 by Quikchange site-directed mutagenesis, and sequence encompassing these mutations sub-cloned by restriction with Pst I and Nco I into pHAM-sgA1/DIII-HA.

    Activity Assay:

    Article Title: Pharmacokinetic-Pharmacodynamic Analysis of Spiroindolone Analogs and KAE609 in a Murine Malaria Model
    Article Snippet: .. In vitro antimalarial activity was measured using the [3 H]hypoxanthine incorporation assay ( ) with strain NF54 of P. falciparum (obtained from Hoffmann-LaRoche Ltd.). .. The compounds were dissolved in dimethyl sulfoxide (DMSO) at a concentration of 10 mM, diluted in hypoxanthine-free culture medium, and titrated in duplicate over a 64-fold range in 96-well plates.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86
    Roche p falciparum
    P Falciparum, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p falciparum/product/Roche
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    p falciparum - by Bioz Stars, 2021-05
    86/100 stars
      Buy from Supplier

    86
    Roche 3d7 strain p falciparum merozoites
    3d7 Strain P Falciparum Merozoites, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/3d7 strain p falciparum merozoites/product/Roche
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    3d7 strain p falciparum merozoites - by Bioz Stars, 2021-05
    86/100 stars
      Buy from Supplier

    Image Search Results