kv4 2 (Alomone Labs)


Structured Review

Kv4 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/kv4 2/product/Alomone Labs
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "The role of voltage-gated potassium channels in the regulation of mouse uterine contractility"
Article Title: The role of voltage-gated potassium channels in the regulation of mouse uterine contractility
Journal: Reproductive biology and endocrinology : RB&E
doi: 10.1186/1477-7827-5-41

Figure Legend Snippet: Kv channel α subunit proteins are expressed in mouse myometrium . Results obtained from Western blots demonstrating the presence of immunoreactive bands for Kv1.1, Kv1.2, Kv1.3, Kv1.4, Kv1.5, Kv1.6, and Kv4.2 α-subunits in myometrium from nonpregnant (NP) and term-pregnant (P20) mice. Kv4.3 α-subunits were detected in the NP samples, but were undetectable in the term-pregnant mouse myometrium. Apparent molecular weights of each protein species are indicated at right. Different lanes contain samples from different animals, and each lane was loaded with 20 μg protein; 4–7 animals were assayed for each Kv channel α subunit protein.
Techniques Used: Western Blot

Figure Legend Snippet: Time course of loss of 4-AP responsiveness and Kv4.3 expression in pregnant mouse myometrium . Myometrial samples were obtained from nonpregnant (NP, n = 5), 7 days pregnant (P7, n = 3), 10 days pregnant (P10, n = 3), 15 days pregnant (P15, n = 4) and term-pregnant (P20, n = 5) mice. Panel A: Results from non-cumulative concentration-response experiments are presented as mean percentages of the maximal response to 4-AP in NP tissues. Panel B: Densitometry of Kv4.3 expression in the same samples evaluated in panel (A) and typical Western blot results demonstrating expression of Kv4.3 as well as Kv1.4 and γ-actin to confirm equal protein loading. Band densities are expressed in graph as a percentage of the maximal band density on each film; asterisks denote a significant difference (P = 0.01) between average NP and term-pregnant sample band densities.
Techniques Used: Expressing, Concentration Assay, Western Blot
kv4 2 (Alomone Labs)


Structured Review

Kv4 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/kv4 2/product/Alomone Labs
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "The role of voltage-gated potassium channels in the regulation of mouse uterine contractility"
Article Title: The role of voltage-gated potassium channels in the regulation of mouse uterine contractility
Journal: Reproductive biology and endocrinology : RB&E
doi: 10.1186/1477-7827-5-41

Figure Legend Snippet: Kv channel α subunit proteins are expressed in mouse myometrium . Results obtained from Western blots demonstrating the presence of immunoreactive bands for Kv1.1, Kv1.2, Kv1.3, Kv1.4, Kv1.5, Kv1.6, and Kv4.2 α-subunits in myometrium from nonpregnant (NP) and term-pregnant (P20) mice. Kv4.3 α-subunits were detected in the NP samples, but were undetectable in the term-pregnant mouse myometrium. Apparent molecular weights of each protein species are indicated at right. Different lanes contain samples from different animals, and each lane was loaded with 20 μg protein; 4–7 animals were assayed for each Kv channel α subunit protein.
Techniques Used: Western Blot

Figure Legend Snippet: Time course of loss of 4-AP responsiveness and Kv4.3 expression in pregnant mouse myometrium . Myometrial samples were obtained from nonpregnant (NP, n = 5), 7 days pregnant (P7, n = 3), 10 days pregnant (P10, n = 3), 15 days pregnant (P15, n = 4) and term-pregnant (P20, n = 5) mice. Panel A: Results from non-cumulative concentration-response experiments are presented as mean percentages of the maximal response to 4-AP in NP tissues. Panel B: Densitometry of Kv4.3 expression in the same samples evaluated in panel (A) and typical Western blot results demonstrating expression of Kv4.3 as well as Kv1.4 and γ-actin to confirm equal protein loading. Band densities are expressed in graph as a percentage of the maximal band density on each film; asterisks denote a significant difference (P = 0.01) between average NP and term-pregnant sample band densities.
Techniques Used: Expressing, Concentration Assay, Western Blot
rabbit polyclonal (Alomone Labs)


Structured Review
Rabbit Polyclonal, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit polyclonal/product/Alomone Labs
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
anti kv4 2 (Alomone Labs)


Structured Review
Anti Kv4 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti kv4 2/product/Alomone Labs
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
rabbit polyclonal (Alomone Labs)


Structured Review
Rabbit Polyclonal, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit polyclonal/product/Alomone Labs
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
rabbit polyclonal (Alomone Labs)


Structured Review
Rabbit Polyclonal, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit polyclonal/product/Alomone Labs
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
anti kv4 2 (Alomone Labs)


Structured Review
Anti Kv4 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti kv4 2/product/Alomone Labs
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
rabbit α kv4 2 (Alomone Labs)


Structured Review

Rabbit α Kv4 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit α kv4 2/product/Alomone Labs
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Physiological Characterization of Vestibular Efferent Brainstem Neurons Using a Transgenic Mouse Model"
Article Title: Physiological Characterization of Vestibular Efferent Brainstem Neurons Using a Transgenic Mouse Model
Journal: PLoS ONE
doi: 10.1371/journal.pone.0098277

Figure Legend Snippet: Primary antibodies used.
Techniques Used:

Figure Legend Snippet: Adult mouse immunofluorescently labeled against ChAT (red) and Kv4.2 or Kv4.3 α-subunits (green) using shock-frozen tissue. The ChAT labeling overlaps with the Kv4.3 labeling in the mouse VE neurons (A–C). Likewise, both the ChAT-positive LOC efferent cells (arrows) and the principal cells of the lateral superior olive (arrowheads) show Kv4.3 expression in the mouse (D–F). In a similar fashion, both the VE neurons (G–I) and the LOC efferent cells (arrows) and the principal cells of the lateral superior olive (arrowheads) (J–L) are immunolabeled against Kv4.2, which indicate that the Kv4 channels are hetero-meric in these auditory brainstem nuclei. Scale bars: 100 µm.
Techniques Used: Labeling, Expressing, Immunolabeling
rat k v 4 2 (Alomone Labs)


Structured Review
Rat K V 4 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rat k v 4 2/product/Alomone Labs
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Physiological Characterization of Vestibular Efferent Brainstem Neurons Using a Transgenic Mouse Model"
Article Title: Physiological Characterization of Vestibular Efferent Brainstem Neurons Using a Transgenic Mouse Model
Journal: PLoS ONE
doi: 10.1371/journal.pone.0098277
Figure Legend Snippet: Primary antibodies used.
Techniques Used:
apc 023 (Alomone Labs)


Structured Review
Apc 023, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/apc 023/product/Alomone Labs
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
kv4 2 (Alomone Labs)


Structured Review

Kv4 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/kv4 2/product/Alomone Labs
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Physiological Characterization of Vestibular Efferent Brainstem Neurons Using a Transgenic Mouse Model"
Article Title: Physiological Characterization of Vestibular Efferent Brainstem Neurons Using a Transgenic Mouse Model
Journal: PLoS ONE
doi: 10.1371/journal.pone.0098277

Figure Legend Snippet: Primary antibodies used.
Techniques Used:

Figure Legend Snippet: Confocal laser scanning microscopy images of adult rat immunofluorescently labeled against ChAT (red) and Kv4.3 (green). The ChAT labeled VE (A–C) and the LOC (D–E) neurons are double labeled with Kv4.3, indicating that they are expressing the protein of the Kv4.3 α-subunit. Arrows indicate double labeled efferent neurons and arrowheads point to cells labeled against Kv4.3, but lacking ChAT immunolabeling, most probably corresponding to principal neurons in the lateral superior olive (D–E). Scale bars: (A–C) 100 µm, (D–F) 50 µm.
Techniques Used: Confocal Laser Scanning Microscopy, Labeling, Expressing, Immunolabeling

Figure Legend Snippet: Adult mouse immunofluorescently labeled against ChAT (red) and Kv4.2 or Kv4.3 α-subunits (green) using shock-frozen tissue. The ChAT labeling overlaps with the Kv4.3 labeling in the mouse VE neurons (A–C). Likewise, both the ChAT-positive LOC efferent cells (arrows) and the principal cells of the lateral superior olive (arrowheads) show Kv4.3 expression in the mouse (D–F). In a similar fashion, both the VE neurons (G–I) and the LOC efferent cells (arrows) and the principal cells of the lateral superior olive (arrowheads) (J–L) are immunolabeled against Kv4.2, which indicate that the Kv4 channels are hetero-meric in these auditory brainstem nuclei. Scale bars: 100 µm.
Techniques Used: Labeling, Expressing, Immunolabeling