p gsk3 β ser9  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc p gsk3 β ser9
    Leptin mediates β -catenin activation through the crosstalk between MTA1/WNT and PI3K/AKT pathways in HTR-8/SVneo cells. (a) HTR-8/SVneo cells were treated with exogenous leptin (0 and 200 ng/ml) for 24 h, and MTA1, WNT1, <t>p-GSK3</t> β <t>(Ser9),</t> and p-AKT (Ser473) levels were detected by Western blot. Data are shown as mean ± SD; ∗ P < 0.01 vs. leptin (0 ng/ml). (b) The knockdown efficiencies of MTA1 and WNT1 were analyzed by Western blot analysis and qRT-PCR. ∗ P < 0.05 vs. control. (c) HTR-8/SVneo cells were transfected with MTA1 siRNA or scramble siRNA (Scr) in the presence or absence of 200 ng/ml leptin for 24 h, and Western blot analysis was performed to detect the expression of MTA1, WNT1, p-GSK3 β (Ser9), and nuclear β -catenin. ∗ P < 0.01 vs. control and # P < 0.01 vs. leptin. (d) HTR-8/SVneo cells were transfected with WNT1 siRNA or scramble siRNA (Scr) in the presence or absence of 200 ng/ml leptin for 24 h, and Western blot analysis was performed to detect the expression of WNT1, p-GSK3 β (Ser9), and nuclear β -catenin. ∗ P < 0.01 vs. control and # P < 0.01 vs. leptin. (e) The knockdown efficiencies of AKT and β -catenin were analyzed by Western blot analysis and qRT-PCR. ∗ P < 0.05 vs. control. (f) HTR-8/SVneo cells were transfected with AKT siRNA or scramble siRNA (Scr) in the presence or absence of 200 ng/ml leptin for 24 h, and Western blot analysis was performed to detect the expression of AKT, p-GSK3 β (Ser9), and nuclear β -catenin. ∗ P < 0.01 vs. control and # P < 0.01 vs. leptin. All experiments were performed in triplicate.
    P Gsk3 β Ser9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p gsk3 β ser9/product/Cell Signaling Technology Inc
    Average 96 stars, based on 1 article reviews
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    p gsk3 β ser9 - by Bioz Stars, 2023-06
    96/100 stars

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    1) Product Images from "Leptin Promotes HTR-8/SVneo Cell Invasion via the Crosstalk between MTA1/WNT and PI3K/AKT Pathways"

    Article Title: Leptin Promotes HTR-8/SVneo Cell Invasion via the Crosstalk between MTA1/WNT and PI3K/AKT Pathways

    Journal: Disease Markers

    doi: 10.1155/2022/7052176

    Leptin mediates β -catenin activation through the crosstalk between MTA1/WNT and PI3K/AKT pathways in HTR-8/SVneo cells. (a) HTR-8/SVneo cells were treated with exogenous leptin (0 and 200 ng/ml) for 24 h, and MTA1, WNT1, p-GSK3 β (Ser9), and p-AKT (Ser473) levels were detected by Western blot. Data are shown as mean ± SD; ∗ P < 0.01 vs. leptin (0 ng/ml). (b) The knockdown efficiencies of MTA1 and WNT1 were analyzed by Western blot analysis and qRT-PCR. ∗ P < 0.05 vs. control. (c) HTR-8/SVneo cells were transfected with MTA1 siRNA or scramble siRNA (Scr) in the presence or absence of 200 ng/ml leptin for 24 h, and Western blot analysis was performed to detect the expression of MTA1, WNT1, p-GSK3 β (Ser9), and nuclear β -catenin. ∗ P < 0.01 vs. control and # P < 0.01 vs. leptin. (d) HTR-8/SVneo cells were transfected with WNT1 siRNA or scramble siRNA (Scr) in the presence or absence of 200 ng/ml leptin for 24 h, and Western blot analysis was performed to detect the expression of WNT1, p-GSK3 β (Ser9), and nuclear β -catenin. ∗ P < 0.01 vs. control and # P < 0.01 vs. leptin. (e) The knockdown efficiencies of AKT and β -catenin were analyzed by Western blot analysis and qRT-PCR. ∗ P < 0.05 vs. control. (f) HTR-8/SVneo cells were transfected with AKT siRNA or scramble siRNA (Scr) in the presence or absence of 200 ng/ml leptin for 24 h, and Western blot analysis was performed to detect the expression of AKT, p-GSK3 β (Ser9), and nuclear β -catenin. ∗ P < 0.01 vs. control and # P < 0.01 vs. leptin. All experiments were performed in triplicate.
    Figure Legend Snippet: Leptin mediates β -catenin activation through the crosstalk between MTA1/WNT and PI3K/AKT pathways in HTR-8/SVneo cells. (a) HTR-8/SVneo cells were treated with exogenous leptin (0 and 200 ng/ml) for 24 h, and MTA1, WNT1, p-GSK3 β (Ser9), and p-AKT (Ser473) levels were detected by Western blot. Data are shown as mean ± SD; ∗ P < 0.01 vs. leptin (0 ng/ml). (b) The knockdown efficiencies of MTA1 and WNT1 were analyzed by Western blot analysis and qRT-PCR. ∗ P < 0.05 vs. control. (c) HTR-8/SVneo cells were transfected with MTA1 siRNA or scramble siRNA (Scr) in the presence or absence of 200 ng/ml leptin for 24 h, and Western blot analysis was performed to detect the expression of MTA1, WNT1, p-GSK3 β (Ser9), and nuclear β -catenin. ∗ P < 0.01 vs. control and # P < 0.01 vs. leptin. (d) HTR-8/SVneo cells were transfected with WNT1 siRNA or scramble siRNA (Scr) in the presence or absence of 200 ng/ml leptin for 24 h, and Western blot analysis was performed to detect the expression of WNT1, p-GSK3 β (Ser9), and nuclear β -catenin. ∗ P < 0.01 vs. control and # P < 0.01 vs. leptin. (e) The knockdown efficiencies of AKT and β -catenin were analyzed by Western blot analysis and qRT-PCR. ∗ P < 0.05 vs. control. (f) HTR-8/SVneo cells were transfected with AKT siRNA or scramble siRNA (Scr) in the presence or absence of 200 ng/ml leptin for 24 h, and Western blot analysis was performed to detect the expression of AKT, p-GSK3 β (Ser9), and nuclear β -catenin. ∗ P < 0.01 vs. control and # P < 0.01 vs. leptin. All experiments were performed in triplicate.

    Techniques Used: Activation Assay, Western Blot, Quantitative RT-PCR, Transfection, Expressing

    p gsk3 β ser9  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc p gsk3 β ser9
    Leptin mediates β -catenin activation through the crosstalk between MTA1/WNT and PI3K/AKT pathways in HTR-8/SVneo cells. (a) HTR-8/SVneo cells were treated with exogenous leptin (0 and 200 ng/ml) for 24 h, and MTA1, WNT1, <t>p-GSK3</t> β <t>(Ser9),</t> and p-AKT (Ser473) levels were detected by Western blot. Data are shown as mean ± SD; ∗ P < 0.01 vs. leptin (0 ng/ml). (b) The knockdown efficiencies of MTA1 and WNT1 were analyzed by Western blot analysis and qRT-PCR. ∗ P < 0.05 vs. control. (c) HTR-8/SVneo cells were transfected with MTA1 siRNA or scramble siRNA (Scr) in the presence or absence of 200 ng/ml leptin for 24 h, and Western blot analysis was performed to detect the expression of MTA1, WNT1, p-GSK3 β (Ser9), and nuclear β -catenin. ∗ P < 0.01 vs. control and # P < 0.01 vs. leptin. (d) HTR-8/SVneo cells were transfected with WNT1 siRNA or scramble siRNA (Scr) in the presence or absence of 200 ng/ml leptin for 24 h, and Western blot analysis was performed to detect the expression of WNT1, p-GSK3 β (Ser9), and nuclear β -catenin. ∗ P < 0.01 vs. control and # P < 0.01 vs. leptin. (e) The knockdown efficiencies of AKT and β -catenin were analyzed by Western blot analysis and qRT-PCR. ∗ P < 0.05 vs. control. (f) HTR-8/SVneo cells were transfected with AKT siRNA or scramble siRNA (Scr) in the presence or absence of 200 ng/ml leptin for 24 h, and Western blot analysis was performed to detect the expression of AKT, p-GSK3 β (Ser9), and nuclear β -catenin. ∗ P < 0.01 vs. control and # P < 0.01 vs. leptin. All experiments were performed in triplicate.
    P Gsk3 β Ser9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p gsk3 β ser9/product/Cell Signaling Technology Inc
    Average 96 stars, based on 1 article reviews
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    p gsk3 β ser9 - by Bioz Stars, 2023-06
    96/100 stars

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    1) Product Images from "Leptin Promotes HTR-8/SVneo Cell Invasion via the Crosstalk between MTA1/WNT and PI3K/AKT Pathways"

    Article Title: Leptin Promotes HTR-8/SVneo Cell Invasion via the Crosstalk between MTA1/WNT and PI3K/AKT Pathways

    Journal: Disease Markers

    doi: 10.1155/2022/7052176

    Leptin mediates β -catenin activation through the crosstalk between MTA1/WNT and PI3K/AKT pathways in HTR-8/SVneo cells. (a) HTR-8/SVneo cells were treated with exogenous leptin (0 and 200 ng/ml) for 24 h, and MTA1, WNT1, p-GSK3 β (Ser9), and p-AKT (Ser473) levels were detected by Western blot. Data are shown as mean ± SD; ∗ P < 0.01 vs. leptin (0 ng/ml). (b) The knockdown efficiencies of MTA1 and WNT1 were analyzed by Western blot analysis and qRT-PCR. ∗ P < 0.05 vs. control. (c) HTR-8/SVneo cells were transfected with MTA1 siRNA or scramble siRNA (Scr) in the presence or absence of 200 ng/ml leptin for 24 h, and Western blot analysis was performed to detect the expression of MTA1, WNT1, p-GSK3 β (Ser9), and nuclear β -catenin. ∗ P < 0.01 vs. control and # P < 0.01 vs. leptin. (d) HTR-8/SVneo cells were transfected with WNT1 siRNA or scramble siRNA (Scr) in the presence or absence of 200 ng/ml leptin for 24 h, and Western blot analysis was performed to detect the expression of WNT1, p-GSK3 β (Ser9), and nuclear β -catenin. ∗ P < 0.01 vs. control and # P < 0.01 vs. leptin. (e) The knockdown efficiencies of AKT and β -catenin were analyzed by Western blot analysis and qRT-PCR. ∗ P < 0.05 vs. control. (f) HTR-8/SVneo cells were transfected with AKT siRNA or scramble siRNA (Scr) in the presence or absence of 200 ng/ml leptin for 24 h, and Western blot analysis was performed to detect the expression of AKT, p-GSK3 β (Ser9), and nuclear β -catenin. ∗ P < 0.01 vs. control and # P < 0.01 vs. leptin. All experiments were performed in triplicate.
    Figure Legend Snippet: Leptin mediates β -catenin activation through the crosstalk between MTA1/WNT and PI3K/AKT pathways in HTR-8/SVneo cells. (a) HTR-8/SVneo cells were treated with exogenous leptin (0 and 200 ng/ml) for 24 h, and MTA1, WNT1, p-GSK3 β (Ser9), and p-AKT (Ser473) levels were detected by Western blot. Data are shown as mean ± SD; ∗ P < 0.01 vs. leptin (0 ng/ml). (b) The knockdown efficiencies of MTA1 and WNT1 were analyzed by Western blot analysis and qRT-PCR. ∗ P < 0.05 vs. control. (c) HTR-8/SVneo cells were transfected with MTA1 siRNA or scramble siRNA (Scr) in the presence or absence of 200 ng/ml leptin for 24 h, and Western blot analysis was performed to detect the expression of MTA1, WNT1, p-GSK3 β (Ser9), and nuclear β -catenin. ∗ P < 0.01 vs. control and # P < 0.01 vs. leptin. (d) HTR-8/SVneo cells were transfected with WNT1 siRNA or scramble siRNA (Scr) in the presence or absence of 200 ng/ml leptin for 24 h, and Western blot analysis was performed to detect the expression of WNT1, p-GSK3 β (Ser9), and nuclear β -catenin. ∗ P < 0.01 vs. control and # P < 0.01 vs. leptin. (e) The knockdown efficiencies of AKT and β -catenin were analyzed by Western blot analysis and qRT-PCR. ∗ P < 0.05 vs. control. (f) HTR-8/SVneo cells were transfected with AKT siRNA or scramble siRNA (Scr) in the presence or absence of 200 ng/ml leptin for 24 h, and Western blot analysis was performed to detect the expression of AKT, p-GSK3 β (Ser9), and nuclear β -catenin. ∗ P < 0.01 vs. control and # P < 0.01 vs. leptin. All experiments were performed in triplicate.

    Techniques Used: Activation Assay, Western Blot, Quantitative RT-PCR, Transfection, Expressing

    p gsk3 β  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc p gsk3 β
    Protein expression in GA muscle samples from rats treated with n-3 PUFA or vehicle for 40 days and dexamethasone or vehicle (last 10 days). Relative optical density of total Akt (tAkt) and phosphorylated Akt (P-Akt) ((a) and (c)), total <t>GSK3</t> <t>β</t> (tGSK3 β ) and phosphorylated GSK3 β (P-GSK3 β ) ((b) and (d)), and tAkt/P-Akt and tGSK3 β /P-GSK3 β ratios ((e) and (f), resp.). Bands representing tAkt, P-Akt, tGSK3, and P-GSK3 β are illustrated in (g). α -Tubulin (55 kDa) was used as control. The numbers represent the mean ± S.E.M. CT = control group ( n = 6), DX = dexamethasone group ( n = 5), n-3 = n-3 PUFA group ( n = 6), and DX + n-3 = dexamethasone + n-3 PUFA group ( n = 6). Values represent means ± S.E.M., analyzed by Student's t -test, # P < 0.05.
    P Gsk3 β, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "The Effects of Omega-3 Fatty Acid Supplementation on Dexamethasone-Induced Muscle Atrophy"

    Article Title: The Effects of Omega-3 Fatty Acid Supplementation on Dexamethasone-Induced Muscle Atrophy

    Journal: BioMed Research International

    doi: 10.1155/2014/961438

    Protein expression in GA muscle samples from rats treated with n-3 PUFA or vehicle for 40 days and dexamethasone or vehicle (last 10 days). Relative optical density of total Akt (tAkt) and phosphorylated Akt (P-Akt) ((a) and (c)), total GSK3 β (tGSK3 β ) and phosphorylated GSK3 β (P-GSK3 β ) ((b) and (d)), and tAkt/P-Akt and tGSK3 β /P-GSK3 β ratios ((e) and (f), resp.). Bands representing tAkt, P-Akt, tGSK3, and P-GSK3 β are illustrated in (g). α -Tubulin (55 kDa) was used as control. The numbers represent the mean ± S.E.M. CT = control group ( n = 6), DX = dexamethasone group ( n = 5), n-3 = n-3 PUFA group ( n = 6), and DX + n-3 = dexamethasone + n-3 PUFA group ( n = 6). Values represent means ± S.E.M., analyzed by Student's t -test, # P < 0.05.
    Figure Legend Snippet: Protein expression in GA muscle samples from rats treated with n-3 PUFA or vehicle for 40 days and dexamethasone or vehicle (last 10 days). Relative optical density of total Akt (tAkt) and phosphorylated Akt (P-Akt) ((a) and (c)), total GSK3 β (tGSK3 β ) and phosphorylated GSK3 β (P-GSK3 β ) ((b) and (d)), and tAkt/P-Akt and tGSK3 β /P-GSK3 β ratios ((e) and (f), resp.). Bands representing tAkt, P-Akt, tGSK3, and P-GSK3 β are illustrated in (g). α -Tubulin (55 kDa) was used as control. The numbers represent the mean ± S.E.M. CT = control group ( n = 6), DX = dexamethasone group ( n = 5), n-3 = n-3 PUFA group ( n = 6), and DX + n-3 = dexamethasone + n-3 PUFA group ( n = 6). Values represent means ± S.E.M., analyzed by Student's t -test, # P < 0.05.

    Techniques Used: Expressing

    p gsk3 β  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc p gsk3 β
    Antibody details for western immunoblotting.
    P Gsk3 β, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 96 stars, based on 1 article reviews
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    p gsk3 β - by Bioz Stars, 2023-06
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    1) Product Images from "Preserved Left Ventricular Function despite Myocardial Fibrosis and Myopathy in the Dystrophin-Deficient D2.B10-Dmd mdx /J Mouse"

    Article Title: Preserved Left Ventricular Function despite Myocardial Fibrosis and Myopathy in the Dystrophin-Deficient D2.B10-Dmd mdx /J Mouse

    Journal: Oxidative Medicine and Cellular Longevity

    doi: 10.1155/2022/5362115

    Antibody details for western immunoblotting.
    Figure Legend Snippet: Antibody details for western immunoblotting.

    Techniques Used: Western Blot, Molecular Weight

    p gsk3 β  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
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    Cell Signaling Technology Inc p gsk3 β
    P Gsk3 β, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p gsk3 β/product/Cell Signaling Technology Inc
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    p gsk3 β - by Bioz Stars, 2023-06
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    p gsk3 β  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc p gsk3 β
    Wogonin improves the IL-10 production in MSCs via <t>GSK3</t> <t>β</t> /AKT pathway. MSCs were stimulated by Wogonin at a different dose (12.5, 25, and 50 μ M) in the presence of LPS. After 24 h stimulation, western blot was performed. The cell extract probed with antibodies as indicated, and GAPDH as loading control. Relative protein expression was calculated, respectively. (a) The representatives of the protein expression of HIF-1 α , IL-10 as well as GSK3 β , AKT, and Stat3 and their phosphorylated forms (p-GSK3 β , p-AKT, and p-Stat3). The effects of Wogonin on the expression of HIF-1 α and IL-10. (b) The effects of Wogonin on GSK3 β . (c) AKT (d) and Stat3 (e) signaling pathways. All the western blotting experiments were repeated three times. Values are means ± SEM. The statistical significance of difference between two means was calculated with two-way ANOVA followed by multiple comparisons with t test and Bonferroni correction, ∗ P < 0.05.
    P Gsk3 β, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 96 stars, based on 1 article reviews
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    p gsk3 β - by Bioz Stars, 2023-06
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    1) Product Images from "Wogonin Strengthens the Therapeutic Effects of Mesenchymal Stem Cells in DSS-Induced Colitis via Promoting IL-10 Production"

    Article Title: Wogonin Strengthens the Therapeutic Effects of Mesenchymal Stem Cells in DSS-Induced Colitis via Promoting IL-10 Production

    Journal: Oxidative Medicine and Cellular Longevity

    doi: 10.1155/2021/5527935

    Wogonin improves the IL-10 production in MSCs via GSK3 β /AKT pathway. MSCs were stimulated by Wogonin at a different dose (12.5, 25, and 50 μ M) in the presence of LPS. After 24 h stimulation, western blot was performed. The cell extract probed with antibodies as indicated, and GAPDH as loading control. Relative protein expression was calculated, respectively. (a) The representatives of the protein expression of HIF-1 α , IL-10 as well as GSK3 β , AKT, and Stat3 and their phosphorylated forms (p-GSK3 β , p-AKT, and p-Stat3). The effects of Wogonin on the expression of HIF-1 α and IL-10. (b) The effects of Wogonin on GSK3 β . (c) AKT (d) and Stat3 (e) signaling pathways. All the western blotting experiments were repeated three times. Values are means ± SEM. The statistical significance of difference between two means was calculated with two-way ANOVA followed by multiple comparisons with t test and Bonferroni correction, ∗ P < 0.05.
    Figure Legend Snippet: Wogonin improves the IL-10 production in MSCs via GSK3 β /AKT pathway. MSCs were stimulated by Wogonin at a different dose (12.5, 25, and 50 μ M) in the presence of LPS. After 24 h stimulation, western blot was performed. The cell extract probed with antibodies as indicated, and GAPDH as loading control. Relative protein expression was calculated, respectively. (a) The representatives of the protein expression of HIF-1 α , IL-10 as well as GSK3 β , AKT, and Stat3 and their phosphorylated forms (p-GSK3 β , p-AKT, and p-Stat3). The effects of Wogonin on the expression of HIF-1 α and IL-10. (b) The effects of Wogonin on GSK3 β . (c) AKT (d) and Stat3 (e) signaling pathways. All the western blotting experiments were repeated three times. Values are means ± SEM. The statistical significance of difference between two means was calculated with two-way ANOVA followed by multiple comparisons with t test and Bonferroni correction, ∗ P < 0.05.

    Techniques Used: Western Blot, Expressing

    p gsk3 β  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc p gsk3 β
    Primers used for real-time quantitative polymerase chain reaction.
    P Gsk3 β, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    1) Product Images from "Resveratrol Alleviates Skeletal Muscle Insulin Resistance by Downregulating Long Noncoding RNA"

    Article Title: Resveratrol Alleviates Skeletal Muscle Insulin Resistance by Downregulating Long Noncoding RNA

    Journal: International Journal of Endocrinology

    doi: 10.1155/2022/2539519

    Primers used for real-time quantitative polymerase chain reaction.
    Figure Legend Snippet: Primers used for real-time quantitative polymerase chain reaction.

    Techniques Used:

    Effects of resveratrol on the insulin signaling pathway in control, HFD, and HFD + RSV groups. (a) Bands of western blot; (b) AKT; (c) p-AKT; (d) GSK3 β ; (e) p-GSK3 β ; (f) SOCS1. Data are expressed as the mean ± SD ( n = 6). ∗ P < 0.05 versus CON; # P < 0.05 versus HFD.
    Figure Legend Snippet: Effects of resveratrol on the insulin signaling pathway in control, HFD, and HFD + RSV groups. (a) Bands of western blot; (b) AKT; (c) p-AKT; (d) GSK3 β ; (e) p-GSK3 β ; (f) SOCS1. Data are expressed as the mean ± SD ( n = 6). ∗ P < 0.05 versus CON; # P < 0.05 versus HFD.

    Techniques Used: Western Blot

    Resveratrol improved skeletal muscle insulin resistance by downregulating the lncRNA NONMUT044897.2 in vitro . Validation of lncRNAs NONMMUT044897.2 (a), NONMMUT139818.1 (b), NONMMUT00000181045(c), NONMMUT071570.2 (d), and NONMMUT06516.2 (e) by RT-qPCR. Expression of miR-7051-5p mRNA (f) and NONMMUT044897.2 mRNA (g) after shRNA transfection into C2C12 cells in different groups. (h) Glucose concentrations in the culture medium after shRNA transfection into C2C12 cells in different groups. (i) Protein bands of insulin signaling pathway-related molecules. Densitometric analysis of (j) AKT, (k) p-AKT, (l) GSK3 β , (m) p-GSK3 β , (n) GLUT4, and (o) SOCS1. Data are presented as the mean ± SD ( n = 6). ∗ P < 0.05 versus CON, # P < 0.05 versus PA, and δ P < 0.05 versus PA + shRNA-NONMMUT044897.2.
    Figure Legend Snippet: Resveratrol improved skeletal muscle insulin resistance by downregulating the lncRNA NONMUT044897.2 in vitro . Validation of lncRNAs NONMMUT044897.2 (a), NONMMUT139818.1 (b), NONMMUT00000181045(c), NONMMUT071570.2 (d), and NONMMUT06516.2 (e) by RT-qPCR. Expression of miR-7051-5p mRNA (f) and NONMMUT044897.2 mRNA (g) after shRNA transfection into C2C12 cells in different groups. (h) Glucose concentrations in the culture medium after shRNA transfection into C2C12 cells in different groups. (i) Protein bands of insulin signaling pathway-related molecules. Densitometric analysis of (j) AKT, (k) p-AKT, (l) GSK3 β , (m) p-GSK3 β , (n) GLUT4, and (o) SOCS1. Data are presented as the mean ± SD ( n = 6). ∗ P < 0.05 versus CON, # P < 0.05 versus PA, and δ P < 0.05 versus PA + shRNA-NONMMUT044897.2.

    Techniques Used: In Vitro, Quantitative RT-PCR, Expressing, shRNA, Transfection

    p gsk3 β ser9  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc p gsk3 β ser9
    Hinokitiol inhibited <t>GSK3</t> β -mediated autophagy flux. (a, b) Venn diagram and PPI network diagram of hubgene. (c) Molecular docking: Hinokitiol potentially binding to the molecular of GSK3 β . (d) Western blotting analysis: Hinokitiol inhibited GSK3 β as demonstrated. Quantitative analysis of each bands was represented in numerical form under images, respectively.
    P Gsk3 β Ser9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Hinokitiol Protects Cardiomyocyte from Oxidative Damage by Inhibiting GSK3 β -Mediated Autophagy"

    Article Title: Hinokitiol Protects Cardiomyocyte from Oxidative Damage by Inhibiting GSK3 β -Mediated Autophagy

    Journal: Oxidative Medicine and Cellular Longevity

    doi: 10.1155/2022/2700000

    Hinokitiol inhibited GSK3 β -mediated autophagy flux. (a, b) Venn diagram and PPI network diagram of hubgene. (c) Molecular docking: Hinokitiol potentially binding to the molecular of GSK3 β . (d) Western blotting analysis: Hinokitiol inhibited GSK3 β as demonstrated. Quantitative analysis of each bands was represented in numerical form under images, respectively.
    Figure Legend Snippet: Hinokitiol inhibited GSK3 β -mediated autophagy flux. (a, b) Venn diagram and PPI network diagram of hubgene. (c) Molecular docking: Hinokitiol potentially binding to the molecular of GSK3 β . (d) Western blotting analysis: Hinokitiol inhibited GSK3 β as demonstrated. Quantitative analysis of each bands was represented in numerical form under images, respectively.

    Techniques Used: Binding Assay, Western Blot

    Hinokitiol increased p21 expression by inhibited GSK3 β . (a) GSVA enrichment of GSE5406. (b) SVM-RFE algorithms in GSE5406 to predict the key genes responsible for heart failure after MI. (c) Partial least square discriminant analysis (PCA) for GSE5406 datasets to identify the vital genes responsible for heart failure after MI. (d) Volcano plot of fold changes of genes with human failing LV myocardium comparison with human nonfailing LV myocardium after MI. p values were calculated using the Wald test. (e) Western blotting analysis: Hinokitiol increase p21 expression as demonstrated. Quantitative analysis of each bands was represented in numerical form under images, respectively.
    Figure Legend Snippet: Hinokitiol increased p21 expression by inhibited GSK3 β . (a) GSVA enrichment of GSE5406. (b) SVM-RFE algorithms in GSE5406 to predict the key genes responsible for heart failure after MI. (c) Partial least square discriminant analysis (PCA) for GSE5406 datasets to identify the vital genes responsible for heart failure after MI. (d) Volcano plot of fold changes of genes with human failing LV myocardium comparison with human nonfailing LV myocardium after MI. p values were calculated using the Wald test. (e) Western blotting analysis: Hinokitiol increase p21 expression as demonstrated. Quantitative analysis of each bands was represented in numerical form under images, respectively.

    Techniques Used: Expressing, Western Blot

    Hinokitiol inhibited GSK3 β -mediated autophagy flux. Enriched Gene Ontology (GO) of Hinokitiol.
    Figure Legend Snippet: Hinokitiol inhibited GSK3 β -mediated autophagy flux. Enriched Gene Ontology (GO) of Hinokitiol.

    Techniques Used:

    p gsk3 β  (Cell Signaling Technology Inc)


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    IGF-1 expression and signaling in the hippocampus of exercise-trained 3xTg-AD mice. (a) Representative immunoblots for IGF-1R and MAPK. (b) IGF-1 concentration in hippocampal homogenate measured by ELISA. (c) p-IGF-1R normalized to total IGF-1R. (d) p-MAPK normalized to MAPK. (e) Representative immunoblots for Akt and <t>GSK3</t> <t>β</t> . (f) p-Akt normalized to Akt. (g) p-GSK3 β normalized to total GSK3 β . Data presented as mean ± SE. Tissues assayed from the nonexercised group (Tg), the aerobic-trained group (Tg+AT), or the resistance-trained group (Tg+RT) ( n = 10/group). Differences determined by one-way ANOVA. ∗ p < 0.05 and ∗∗ p < 0.01.
    P Gsk3 β, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Hippocampal Growth Factor and Myokine Cathepsin B Expression following Aerobic and Resistance Training in 3xTg-AD Mice"

    Article Title: Hippocampal Growth Factor and Myokine Cathepsin B Expression following Aerobic and Resistance Training in 3xTg-AD Mice

    Journal: International Journal of Chronic Diseases

    doi: 10.1155/2020/5919501

    IGF-1 expression and signaling in the hippocampus of exercise-trained 3xTg-AD mice. (a) Representative immunoblots for IGF-1R and MAPK. (b) IGF-1 concentration in hippocampal homogenate measured by ELISA. (c) p-IGF-1R normalized to total IGF-1R. (d) p-MAPK normalized to MAPK. (e) Representative immunoblots for Akt and GSK3 β . (f) p-Akt normalized to Akt. (g) p-GSK3 β normalized to total GSK3 β . Data presented as mean ± SE. Tissues assayed from the nonexercised group (Tg), the aerobic-trained group (Tg+AT), or the resistance-trained group (Tg+RT) ( n = 10/group). Differences determined by one-way ANOVA. ∗ p < 0.05 and ∗∗ p < 0.01.
    Figure Legend Snippet: IGF-1 expression and signaling in the hippocampus of exercise-trained 3xTg-AD mice. (a) Representative immunoblots for IGF-1R and MAPK. (b) IGF-1 concentration in hippocampal homogenate measured by ELISA. (c) p-IGF-1R normalized to total IGF-1R. (d) p-MAPK normalized to MAPK. (e) Representative immunoblots for Akt and GSK3 β . (f) p-Akt normalized to Akt. (g) p-GSK3 β normalized to total GSK3 β . Data presented as mean ± SE. Tissues assayed from the nonexercised group (Tg), the aerobic-trained group (Tg+AT), or the resistance-trained group (Tg+RT) ( n = 10/group). Differences determined by one-way ANOVA. ∗ p < 0.05 and ∗∗ p < 0.01.

    Techniques Used: Expressing, Western Blot, Concentration Assay, Enzyme-linked Immunosorbent Assay

    p gsk3 β ser9  (Cell Signaling Technology Inc)


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    Leptin mediates β -catenin activation through the crosstalk between MTA1/WNT and PI3K/AKT pathways in HTR-8/SVneo cells. (a) HTR-8/SVneo cells were treated with exogenous leptin (0 and 200 ng/ml) for 24 h, and MTA1, WNT1, <t>p-GSK3</t> β <t>(Ser9),</t> and p-AKT (Ser473) levels were detected by Western blot. Data are shown as mean ± SD; ∗ P < 0.01 vs. leptin (0 ng/ml). (b) The knockdown efficiencies of MTA1 and WNT1 were analyzed by Western blot analysis and qRT-PCR. ∗ P < 0.05 vs. control. (c) HTR-8/SVneo cells were transfected with MTA1 siRNA or scramble siRNA (Scr) in the presence or absence of 200 ng/ml leptin for 24 h, and Western blot analysis was performed to detect the expression of MTA1, WNT1, p-GSK3 β (Ser9), and nuclear β -catenin. ∗ P < 0.01 vs. control and # P < 0.01 vs. leptin. (d) HTR-8/SVneo cells were transfected with WNT1 siRNA or scramble siRNA (Scr) in the presence or absence of 200 ng/ml leptin for 24 h, and Western blot analysis was performed to detect the expression of WNT1, p-GSK3 β (Ser9), and nuclear β -catenin. ∗ P < 0.01 vs. control and # P < 0.01 vs. leptin. (e) The knockdown efficiencies of AKT and β -catenin were analyzed by Western blot analysis and qRT-PCR. ∗ P < 0.05 vs. control. (f) HTR-8/SVneo cells were transfected with AKT siRNA or scramble siRNA (Scr) in the presence or absence of 200 ng/ml leptin for 24 h, and Western blot analysis was performed to detect the expression of AKT, p-GSK3 β (Ser9), and nuclear β -catenin. ∗ P < 0.01 vs. control and # P < 0.01 vs. leptin. All experiments were performed in triplicate.
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    Protein expression in GA muscle samples from rats treated with n-3 PUFA or vehicle for 40 days and dexamethasone or vehicle (last 10 days). Relative optical density of total Akt (tAkt) and phosphorylated Akt (P-Akt) ((a) and (c)), total <t>GSK3</t> <t>β</t> (tGSK3 β ) and phosphorylated GSK3 β (P-GSK3 β ) ((b) and (d)), and tAkt/P-Akt and tGSK3 β /P-GSK3 β ratios ((e) and (f), resp.). Bands representing tAkt, P-Akt, tGSK3, and P-GSK3 β are illustrated in (g). α -Tubulin (55 kDa) was used as control. The numbers represent the mean ± S.E.M. CT = control group ( n = 6), DX = dexamethasone group ( n = 5), n-3 = n-3 PUFA group ( n = 6), and DX + n-3 = dexamethasone + n-3 PUFA group ( n = 6). Values represent means ± S.E.M., analyzed by Student's t -test, # P < 0.05.
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    Leptin mediates β -catenin activation through the crosstalk between MTA1/WNT and PI3K/AKT pathways in HTR-8/SVneo cells. (a) HTR-8/SVneo cells were treated with exogenous leptin (0 and 200 ng/ml) for 24 h, and MTA1, WNT1, p-GSK3 β (Ser9), and p-AKT (Ser473) levels were detected by Western blot. Data are shown as mean ± SD; ∗ P < 0.01 vs. leptin (0 ng/ml). (b) The knockdown efficiencies of MTA1 and WNT1 were analyzed by Western blot analysis and qRT-PCR. ∗ P < 0.05 vs. control. (c) HTR-8/SVneo cells were transfected with MTA1 siRNA or scramble siRNA (Scr) in the presence or absence of 200 ng/ml leptin for 24 h, and Western blot analysis was performed to detect the expression of MTA1, WNT1, p-GSK3 β (Ser9), and nuclear β -catenin. ∗ P < 0.01 vs. control and # P < 0.01 vs. leptin. (d) HTR-8/SVneo cells were transfected with WNT1 siRNA or scramble siRNA (Scr) in the presence or absence of 200 ng/ml leptin for 24 h, and Western blot analysis was performed to detect the expression of WNT1, p-GSK3 β (Ser9), and nuclear β -catenin. ∗ P < 0.01 vs. control and # P < 0.01 vs. leptin. (e) The knockdown efficiencies of AKT and β -catenin were analyzed by Western blot analysis and qRT-PCR. ∗ P < 0.05 vs. control. (f) HTR-8/SVneo cells were transfected with AKT siRNA or scramble siRNA (Scr) in the presence or absence of 200 ng/ml leptin for 24 h, and Western blot analysis was performed to detect the expression of AKT, p-GSK3 β (Ser9), and nuclear β -catenin. ∗ P < 0.01 vs. control and # P < 0.01 vs. leptin. All experiments were performed in triplicate.

    Journal: Disease Markers

    Article Title: Leptin Promotes HTR-8/SVneo Cell Invasion via the Crosstalk between MTA1/WNT and PI3K/AKT Pathways

    doi: 10.1155/2022/7052176

    Figure Lengend Snippet: Leptin mediates β -catenin activation through the crosstalk between MTA1/WNT and PI3K/AKT pathways in HTR-8/SVneo cells. (a) HTR-8/SVneo cells were treated with exogenous leptin (0 and 200 ng/ml) for 24 h, and MTA1, WNT1, p-GSK3 β (Ser9), and p-AKT (Ser473) levels were detected by Western blot. Data are shown as mean ± SD; ∗ P < 0.01 vs. leptin (0 ng/ml). (b) The knockdown efficiencies of MTA1 and WNT1 were analyzed by Western blot analysis and qRT-PCR. ∗ P < 0.05 vs. control. (c) HTR-8/SVneo cells were transfected with MTA1 siRNA or scramble siRNA (Scr) in the presence or absence of 200 ng/ml leptin for 24 h, and Western blot analysis was performed to detect the expression of MTA1, WNT1, p-GSK3 β (Ser9), and nuclear β -catenin. ∗ P < 0.01 vs. control and # P < 0.01 vs. leptin. (d) HTR-8/SVneo cells were transfected with WNT1 siRNA or scramble siRNA (Scr) in the presence or absence of 200 ng/ml leptin for 24 h, and Western blot analysis was performed to detect the expression of WNT1, p-GSK3 β (Ser9), and nuclear β -catenin. ∗ P < 0.01 vs. control and # P < 0.01 vs. leptin. (e) The knockdown efficiencies of AKT and β -catenin were analyzed by Western blot analysis and qRT-PCR. ∗ P < 0.05 vs. control. (f) HTR-8/SVneo cells were transfected with AKT siRNA or scramble siRNA (Scr) in the presence or absence of 200 ng/ml leptin for 24 h, and Western blot analysis was performed to detect the expression of AKT, p-GSK3 β (Ser9), and nuclear β -catenin. ∗ P < 0.01 vs. control and # P < 0.01 vs. leptin. All experiments were performed in triplicate.

    Article Snippet: The following primary antibodies were used: MMP9 (ab76003), MTA1 (ab71153), WNT1 (ab15251), AKT (ab179463), GSK3 β (ab32391), β -catenin (ab32572), histone 3 (ab1791), β -actin (ab6276) (Abcam, USA); p-AKT (Ser473) (#4060), p-GSK3 β (Ser9) (#9322) (Cell Signaling Technology, USA).

    Techniques: Activation Assay, Western Blot, Quantitative RT-PCR, Transfection, Expressing

    Protein expression in GA muscle samples from rats treated with n-3 PUFA or vehicle for 40 days and dexamethasone or vehicle (last 10 days). Relative optical density of total Akt (tAkt) and phosphorylated Akt (P-Akt) ((a) and (c)), total GSK3 β (tGSK3 β ) and phosphorylated GSK3 β (P-GSK3 β ) ((b) and (d)), and tAkt/P-Akt and tGSK3 β /P-GSK3 β ratios ((e) and (f), resp.). Bands representing tAkt, P-Akt, tGSK3, and P-GSK3 β are illustrated in (g). α -Tubulin (55 kDa) was used as control. The numbers represent the mean ± S.E.M. CT = control group ( n = 6), DX = dexamethasone group ( n = 5), n-3 = n-3 PUFA group ( n = 6), and DX + n-3 = dexamethasone + n-3 PUFA group ( n = 6). Values represent means ± S.E.M., analyzed by Student's t -test, # P < 0.05.

    Journal: BioMed Research International

    Article Title: The Effects of Omega-3 Fatty Acid Supplementation on Dexamethasone-Induced Muscle Atrophy

    doi: 10.1155/2014/961438

    Figure Lengend Snippet: Protein expression in GA muscle samples from rats treated with n-3 PUFA or vehicle for 40 days and dexamethasone or vehicle (last 10 days). Relative optical density of total Akt (tAkt) and phosphorylated Akt (P-Akt) ((a) and (c)), total GSK3 β (tGSK3 β ) and phosphorylated GSK3 β (P-GSK3 β ) ((b) and (d)), and tAkt/P-Akt and tGSK3 β /P-GSK3 β ratios ((e) and (f), resp.). Bands representing tAkt, P-Akt, tGSK3, and P-GSK3 β are illustrated in (g). α -Tubulin (55 kDa) was used as control. The numbers represent the mean ± S.E.M. CT = control group ( n = 6), DX = dexamethasone group ( n = 5), n-3 = n-3 PUFA group ( n = 6), and DX + n-3 = dexamethasone + n-3 PUFA group ( n = 6). Values represent means ± S.E.M., analyzed by Student's t -test, # P < 0.05.

    Article Snippet: The primary antibodies used were Akt (1 : 2,000, Cell Signaling #4691), P-Akt (1 : 2,000, Cell Signaling #4060, Ser473); mTOR (1 : 500, Cell Signaling #2972), P-mTOR (1 : 500, Cell Signaling #2971, Ser2448), GSK3 β (1 : 2,000, Cell Signaling #9315), P-GSK3 β (1 : 2,000, Cell Signaling #9322, Ser9), FoXO3a (1 : 500, Cell Signaling #2497), P-FoXO3a (1 : 500, Cell Signaling #9466, Ser253), and α -tubulin (1 : 30,000, Hybridoma bank) in 5% BSA/TBS-T.

    Techniques: Expressing