Journal: Antioxidants

Article Title: Dietary Curcumin Attenuates Hepatic Cellular Senescence by Suppressing the MAPK/NF-κB Signaling Pathway in Aged Mice

doi: 10.3390/antiox12061165

Figure Lengend Snippet: Curcumin ameliorates hepatic cellular senescence by regulating the p38-MAPK signaling pathway in HFHSD-fed aged mice. Immunoblots and protein expression levels in the liver tissue lysates after 15 weeks of dietary interventions: ( A ) p-ERK1/2, ERK1/2, and p-ERK/ERK ratio; ( B ) p-JNK, JNK, and p-JNK/JNK ratio; ( C ) p-p38, p38, and p-p38/p38 ratio; and ( D ) p-MK2, MK2, and p-MK2/MK2 ratio. The results are expressed as mean ± SEM (* p < 0.05).

Article Snippet: The membranes were blocked in a blocking reagent (LI-COR, Lincoln, NE, USA) at room temperature for 1 h and incubated with the primary antibodies at 4 °C overnight as follows: phosphorylated extracellular signal-regulated kinase (p-ERK) 1/2, ERK1/2, phosphorylated c-Jun N-terminal kinase (p-JNK) 1/2, JNK1/2, p-p38, p38, phosphorylated MAP kinase-activated protein kinase 2 (p-MK2), MK2, p-p65, p65, p53, cyclophilin B, histone deacetylase 1 (HDAC1), β-tubulin, and β-actin from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Western Blot, Expressing

Journal: BioMed Research International

Article Title: Banhasasim-Tang Treatment Reduces the Severity of Esophageal Mucosal Ulcer on Chronic Acid Reflux Esophagitis in Rats

doi: 10.1155/2017/7157212

Figure Lengend Snippet: Effect of BHSST on proinflammatory proteins in chronic acid reflux esophagitis rats. (a) p-p38, p-ERK1/2, and p-JNK protein expressions; (b) c-JUN and NF- κ Bp65 protein expressions; (c) COX-2, iNOS, TNF- α , and IL-6 protein expressions. Normal, normal rats; Control, chronic acid reflux esophagitis rats; BHSST, BHSST 1 g/kg body weight/day-treated chronic acid reflux esophagitis rats. Data are mean ± SEM ( n = 6). Significance: # P < 0.05, ## P < 0.01, and ### P < 0.001 versus normal rats and ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001 versus control rats.

Article Snippet: Rabbit polyclonal antibodies against nuclear factor-kappa B p65 (NF- κ Bp65; 1 : 1,000, SC-372), nuclear factor-erythroid 2-related factor 2 (Nrf2; 1 : 1,000, SC-7228), heme oxygenase-1 (HO-1; 1 : 1,000, SC-10789), superoxide dismutase (SOD; 1 : 1,000, SC-11407), glutathione peroxidase-1/2 (GPx-1/2; 1 : 1,000, SC-30147); goat polyclonal antibodies against Kelch-like ECH-associated protein 1 (Keap1; 1 : 1000, SC-15246), tumor necrosis factor- α (TNF- α ; 1 : 1,000, SC-1351), and interleukin-6 (IL-6; 1 : 1,000, SC-1266) and mouse monoclonal antibodies against phosphor-c-Jun NH 2 -terminal kinase (p-JNK; 1 : 1000, SC-6254), phosphor-extracellular signal-regulated kinase 1/2 (p-ERK1/2; 1 : 1000, SC-7383), phosphor-p38 (p-p38; 1 : 1000, SC-7973), cyclooxygenase-2 (COX-2; 1 : 1,000, SC-19999), inducible nitric oxide synthase (iNOS, 1 : 1,000, SC-7271), histone (1 : 1,000, SC-8030), and β -actin (1 : 1,000, SC-4778) were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).

Techniques: Reflux