p eb2 s222 (Covalab Inc)
Structured Review

P Eb2 S222, supplied by Covalab Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/p eb2 s222/product/Covalab Inc
Average 86 stars, based on 1 article reviews
Images
1) Product Images from "Excitatory Cortical Neurons from CDKL5 Deficiency Disorder Patient-Derived Organoids Show Early Hyperexcitability Not Identified in Neurogenin2 Induced Neurons"
Article Title: Excitatory Cortical Neurons from CDKL5 Deficiency Disorder Patient-Derived Organoids Show Early Hyperexcitability Not Identified in Neurogenin2 Induced Neurons
Journal: bioRxiv
doi: 10.1101/2024.11.11.622878

Figure Legend Snippet: Induced Neurons Model Molecular Consequences of CDKL5 Variants but Display NoTranscriptional, Morphological or Functional Changes. A) Normalized log fold gene expression for iN samples. B) Representative western blots for EB2 and phosphorylation of EB2 S221 with TUJ1/Beta-3-tubulin as a loading control. C) Paired t-test of phosphorylation of EB2 fold change relative to EB2 between isogenic control and CDD patient-derived neurons (N=3 for 2 donors). D) Pearson correlation coefficients for gene expression for the 5000 most variable genes between all samples from iNs. E) Volcano plot of differentially expressed genes between pooled isogenic control and CDD patient-derived samples (N=6 per genotype). F) Representative images of iN neurites immunolabeled with TUJ1 and MAP2 in both patient and isogenic control lines. A paired t-test was performed for changes in neurite length normalized to the cell body area (μm/μm 2 ) for G) MAP2 immunofluorescence (N=3 batches). H) Synchrony index and I) weighted mean firing rate for INs during the first 30 days of maturation post-plating (N=4 differentiation batches, 2 donors). Error bars represent the standard error of the mean.
Techniques Used: Functional Assay, Expressing, Western Blot, Control, Derivative Assay, Immunolabeling, Immunofluorescence

Figure Legend Snippet: No Altered mTOR Signaling in iNs. A) Representative Western blots for phosphorylation of different mTOR-signaling associated proteins. pEB2 and EB2 as shown in for reference. B) Quantification of normalized protein abundance for each tested protein (N=3). Each shape represents a differentiation batch.
Techniques Used: Western Blot

Figure Legend Snippet: Cortical ACTX Neurons with CDKL5 Variants Have Reduced pEB2 and Decreased Expression of CDKL5 . A) Representative western blot of EB2 and pEB2 from ACTX neurons. B) Fold change quantification of western blots for phosphorylation of EB2 relative to EB2 across 3 donors (N = 4 donors). C) Pearson correlations across ACTX neuronal differentiations. D) Differentially expressed genes between pooled isogenic controls and CDD proband-derived neurons (N=7 sample per genotype across 3 donors). E) log2 fold change of CDKL5 and HS3ST1 in ACTX neurons of patient cell lines relative to isogenic controls (N=3, paired t-test). EIF2A is the house-keeping gene.
Techniques Used: Expressing, Western Blot, Derivative Assay