p berghei blood stages  (Millipore)


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    Structured Review

    Millipore p berghei blood stages
    Generation of plasmids and transgenic P. <t>berghei</t> parasites for use in dual-luciferase assays. (A) The vector pFL 103464 RL ef1α with FL under the control of the promoter region 103464 and RL under the control of the ef1α promoter was generated and its transfection resulted in the parasite line PbFL 103464 RL ef1α . The plasmid pFL ef1α RL ef1α with FL and RL under the control of the ef1α promoter was used to obtain control parasites PbFL ef1α RL ef1α . Diamonds represent 3′UTRs, which in all cases were from the pbdhfr/ts gene. In the upper plasmid, the selection marker ( tgdhfr/ts ) is displayed but for simplicity in all other plasmid diagrams only those genes and features directly related to the experiments described are displayed. (B) Comparison of the luciferase activity (FL expression relative to RL expression) of transgenic parasites during the blood stage (BS), in oocysts (Oo), in salivary gland sporozoites (Sp) and in vitro in the liver stage (LS), 48 hours post-infection (hpi) of hepatoma cells. Standard deviation values (shown as error bars) were determined from three different measurements. Statistical analysis was performed using two-tailed unpaired t-tests (*P

    https://www.bioz.com/result/p berghei blood stages/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    p berghei blood stages - by Bioz Stars, 2020-07
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    Images

    1) Product Images from "Identification and Characterization of a Liver Stage-Specific Promoter Region of the Malaria Parasite Plasmodium"

    Article Title: Identification and Characterization of a Liver Stage-Specific Promoter Region of the Malaria Parasite Plasmodium

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0013653

    Generation of plasmids and transgenic P. berghei parasites for use in dual-luciferase assays. (A) The vector pFL 103464 RL ef1α with FL under the control of the promoter region 103464 and RL under the control of the ef1α promoter was generated and its transfection resulted in the parasite line PbFL 103464 RL ef1α . The plasmid pFL ef1α RL ef1α with FL and RL under the control of the ef1α promoter was used to obtain control parasites PbFL ef1α RL ef1α . Diamonds represent 3′UTRs, which in all cases were from the pbdhfr/ts gene. In the upper plasmid, the selection marker ( tgdhfr/ts ) is displayed but for simplicity in all other plasmid diagrams only those genes and features directly related to the experiments described are displayed. (B) Comparison of the luciferase activity (FL expression relative to RL expression) of transgenic parasites during the blood stage (BS), in oocysts (Oo), in salivary gland sporozoites (Sp) and in vitro in the liver stage (LS), 48 hours post-infection (hpi) of hepatoma cells. Standard deviation values (shown as error bars) were determined from three different measurements. Statistical analysis was performed using two-tailed unpaired t-tests (*P
    Figure Legend Snippet: Generation of plasmids and transgenic P. berghei parasites for use in dual-luciferase assays. (A) The vector pFL 103464 RL ef1α with FL under the control of the promoter region 103464 and RL under the control of the ef1α promoter was generated and its transfection resulted in the parasite line PbFL 103464 RL ef1α . The plasmid pFL ef1α RL ef1α with FL and RL under the control of the ef1α promoter was used to obtain control parasites PbFL ef1α RL ef1α . Diamonds represent 3′UTRs, which in all cases were from the pbdhfr/ts gene. In the upper plasmid, the selection marker ( tgdhfr/ts ) is displayed but for simplicity in all other plasmid diagrams only those genes and features directly related to the experiments described are displayed. (B) Comparison of the luciferase activity (FL expression relative to RL expression) of transgenic parasites during the blood stage (BS), in oocysts (Oo), in salivary gland sporozoites (Sp) and in vitro in the liver stage (LS), 48 hours post-infection (hpi) of hepatoma cells. Standard deviation values (shown as error bars) were determined from three different measurements. Statistical analysis was performed using two-tailed unpaired t-tests (*P

    Techniques Used: Transgenic Assay, Luciferase, Plasmid Preparation, Generated, Transfection, Selection, Marker, Activity Assay, Expressing, In Vitro, Infection, Standard Deviation, Two Tailed Test

    Promoter-dependent GFP expression. (A) The vector pGFP 103464 with GFP under the control of the promoter region 103464 and the vector pGFP ef1α with GFP under control of the ef1α promoter were generated and their transfection resulted in the parasite lines PbGFP 103464 and PbGFP ef1α . (B) Live imaging of PbGFP ef1α and PbGFP 103464 parasites at different life cycle stages. HepG2 cells were infected with transgenic P. berghei sporozoites and analyzed at different time points after infection (hpi, hours post-infection). GFP expresion was monitored by fluorescent microscopy. DNA was stained with Hoechst 33342. Arrows indicate young liver stage parasites. (iRBC: infected red blood cell; LS: liver stage) Scale bars: 10 µm.
    Figure Legend Snippet: Promoter-dependent GFP expression. (A) The vector pGFP 103464 with GFP under the control of the promoter region 103464 and the vector pGFP ef1α with GFP under control of the ef1α promoter were generated and their transfection resulted in the parasite lines PbGFP 103464 and PbGFP ef1α . (B) Live imaging of PbGFP ef1α and PbGFP 103464 parasites at different life cycle stages. HepG2 cells were infected with transgenic P. berghei sporozoites and analyzed at different time points after infection (hpi, hours post-infection). GFP expresion was monitored by fluorescent microscopy. DNA was stained with Hoechst 33342. Arrows indicate young liver stage parasites. (iRBC: infected red blood cell; LS: liver stage) Scale bars: 10 µm.

    Techniques Used: Expressing, Plasmid Preparation, Generated, Transfection, Imaging, Infection, Transgenic Assay, Microscopy, Staining

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    Injection:

    Article Title: NPAS2 Contributes to Liver Fibrosis by Direct Transcriptional Activation of Hes1 in Hepatic Stellate Cells
    Article Snippet: .. Male mice aged 6–8 weeks were intraperitoneally injected with CCl4 (0.5 mL/kg body weight, dissolved in olive oil, 1:4; Sigma) or vehicle (olive oil) three times per week for 4 weeks to induce fibrosis and were sacrificed 48 hours after the last injection. .. The mice subjected to BDL were anesthetized with chloral hydrate followed by midline laparotomy.

    Mouse Assay:

    Article Title: The NF-κB regulator Bcl-3 modulates inflammation in contact hypersensitivity reactions in radioresistant cells
    Article Snippet: .. Mice were sensitized with application to shaved bellies of 25 μl of 100 μg/ml of oxazolone (Sigma, dissolved in 1:5 olive oil:acetone) for 2 consecutive days. .. 5 days later, mice were challenged on each side of the ear with application of 5 μl of 10 μg/ml oxazolone or solvent as a control.

    Article Title: NPAS2 Contributes to Liver Fibrosis by Direct Transcriptional Activation of Hes1 in Hepatic Stellate Cells
    Article Snippet: .. Male mice aged 6–8 weeks were intraperitoneally injected with CCl4 (0.5 mL/kg body weight, dissolved in olive oil, 1:4; Sigma) or vehicle (olive oil) three times per week for 4 weeks to induce fibrosis and were sacrificed 48 hours after the last injection. .. The mice subjected to BDL were anesthetized with chloral hydrate followed by midline laparotomy.

    Imaging:

    Article Title: Identification and Characterization of a Liver Stage-Specific Promoter Region of the Malaria Parasite Plasmodium
    Article Snippet: .. For live imaging of P. berghei blood stages, tail blood was taken from an infected mouse, mixed with room temperature cMEM (see above) containing 1 µg/ml Hoechst 33342 (Sigma) and microscopically analyzed. .. Midguts of infected A. stephensi mosquitoes were dissected for live imaging and microscopically investigated.

    Infection:

    Article Title: Identification and Characterization of a Liver Stage-Specific Promoter Region of the Malaria Parasite Plasmodium
    Article Snippet: .. For live imaging of P. berghei blood stages, tail blood was taken from an infected mouse, mixed with room temperature cMEM (see above) containing 1 µg/ml Hoechst 33342 (Sigma) and microscopically analyzed. .. Midguts of infected A. stephensi mosquitoes were dissected for live imaging and microscopically investigated.

    High Content Screening:

    Article Title: An orthotopic mouse model of hepatocellular carcinoma with underlying liver cirrhosis
    Article Snippet: .. Olive oil (Sigma-Aldrich, cat. no. O1514) Ethanol (70% (vol/vol); Pharmco, cat. no. 111000190) HCA-1 cell line (established from a C3H mouse tumor in the Steele Laboratories, Massachusetts General Hospital) ! ..

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  • 85
    Millipore p berghei anka sporozoites
    Trem2 determines KC functional polarization. mRNA quantification of Arg1 ( A ), Il6 ( B ), Ilb1 ( C ), Tnf ( D ), and Cd68 ( E ) in cultured, sort-purified KCs of Trem2 −/− mice relative to C57BL/6, after a 40-h exposure to P. <t>berghei</t> <t>ANKA</t> sporozoites.
    P Berghei Anka Sporozoites, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p berghei anka sporozoites/product/Millipore
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    p berghei anka sporozoites - by Bioz Stars, 2020-07
    85/100 stars
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    85
    Millipore p berghei parasite lysates
    P. <t>berghei</t> induces membranous vesico-tubular structures in the RBC cytsosol. TEM reveals different membranous structures in reticulocytes: The endoplasmatic reticulum, which is characteristic for premature RBCs is indicated by white arrows in uninfected reticulocytes (A+B) and in infected reticulocytes (C+D). The parasite is indicated by ‘P’. Enlargement of the regions boxed in panels A, C and E are shown in panels B, D and F, respectively. Infected RBCs also contain condensed membranous structures within the RBC cytosol (E–I, white arrowheads). Electron tomography performed on an entire infected reticulocyte reveals the 3D architecture of the extra-parasitic membranous structure: G, H and I represent the orthoslices (xy, zy and xz, respectively) of the parasite-induced structure rendered in J. Scale bars: A, C, E 1 mm; B, D, F 200 nm and G–J 100 nm.
    P Berghei Parasite Lysates, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p berghei parasite lysates/product/Millipore
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    p berghei parasite lysates - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

    Image Search Results


    Trem2 determines KC functional polarization. mRNA quantification of Arg1 ( A ), Il6 ( B ), Ilb1 ( C ), Tnf ( D ), and Cd68 ( E ) in cultured, sort-purified KCs of Trem2 −/− mice relative to C57BL/6, after a 40-h exposure to P. berghei ANKA sporozoites.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: TREM2 governs Kupffer cell activation and explains belr1 genetic resistance to malaria liver stage infection

    doi: 10.1073/pnas.1306873110

    Figure Lengend Snippet: Trem2 determines KC functional polarization. mRNA quantification of Arg1 ( A ), Il6 ( B ), Ilb1 ( C ), Tnf ( D ), and Cd68 ( E ) in cultured, sort-purified KCs of Trem2 −/− mice relative to C57BL/6, after a 40-h exposure to P. berghei ANKA sporozoites.

    Article Snippet: KCs in direct contact with hepatocytes were seeded at a 3:1 ratio and infected with 4 × 104 P. berghei ANKA sporozoites after 12 h. In filter-separated culture, KCs were seeded in the upper chamber of a Transwell culture system (Millipore).

    Techniques: Functional Assay, Cell Culture, Purification, Mouse Assay

    P. berghei induces membranous vesico-tubular structures in the RBC cytsosol. TEM reveals different membranous structures in reticulocytes: The endoplasmatic reticulum, which is characteristic for premature RBCs is indicated by white arrows in uninfected reticulocytes (A+B) and in infected reticulocytes (C+D). The parasite is indicated by ‘P’. Enlargement of the regions boxed in panels A, C and E are shown in panels B, D and F, respectively. Infected RBCs also contain condensed membranous structures within the RBC cytosol (E–I, white arrowheads). Electron tomography performed on an entire infected reticulocyte reveals the 3D architecture of the extra-parasitic membranous structure: G, H and I represent the orthoslices (xy, zy and xz, respectively) of the parasite-induced structure rendered in J. Scale bars: A, C, E 1 mm; B, D, F 200 nm and G–J 100 nm.

    Journal: PLoS ONE

    Article Title: The Exported Protein PbCP1 Localises to Cleft-Like Structures in the Rodent Malaria Parasite Plasmodium berghei

    doi: 10.1371/journal.pone.0061482

    Figure Lengend Snippet: P. berghei induces membranous vesico-tubular structures in the RBC cytsosol. TEM reveals different membranous structures in reticulocytes: The endoplasmatic reticulum, which is characteristic for premature RBCs is indicated by white arrows in uninfected reticulocytes (A+B) and in infected reticulocytes (C+D). The parasite is indicated by ‘P’. Enlargement of the regions boxed in panels A, C and E are shown in panels B, D and F, respectively. Infected RBCs also contain condensed membranous structures within the RBC cytosol (E–I, white arrowheads). Electron tomography performed on an entire infected reticulocyte reveals the 3D architecture of the extra-parasitic membranous structure: G, H and I represent the orthoslices (xy, zy and xz, respectively) of the parasite-induced structure rendered in J. Scale bars: A, C, E 1 mm; B, D, F 200 nm and G–J 100 nm.

    Article Snippet: For immunoblots, P. berghei parasite lysates were separated on a 10% SDS-PAGE gel and transferred onto PVDF membrane (Millipore) using SDS transfer buffer with methanol and a wet transfer blotting device (Bio-Rad).

    Techniques: Transmission Electron Microscopy, Infection