p atrosepticum scri1043 genomic dna  (New England Biolabs)


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    NEBuilder HiFi DNA Assembly Bundle for Large Fragments
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    NEBuilder HiFi DNA Assembly Bundle for Large Fragments 20 rxns
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    New England Biolabs p atrosepticum scri1043 genomic dna
    NEBuilder HiFi DNA Assembly Bundle for Large Fragments
    NEBuilder HiFi DNA Assembly Bundle for Large Fragments 20 rxns
    https://www.bioz.com/result/p atrosepticum scri1043 genomic dna/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    p atrosepticum scri1043 genomic dna - by Bioz Stars, 2021-06
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    1) Product Images from "The plant pathogen Pectobacterium atrosepticum contains a functional formate hydrogenlyase‐2 complex"

    Article Title: The plant pathogen Pectobacterium atrosepticum contains a functional formate hydrogenlyase‐2 complex

    Journal: Molecular Microbiology

    doi: 10.1111/mmi.14370

    P. atrosepticum produces molecular hydrogen gas. A. Anaerobic hydrogen production is optimal at lower temperatures. The P. atrosepticum SCRI1043 parent strain was incubated in M9 medium supplemented with 0.8% (w/v) glucose for 168 h at the temperatures indicated before gaseous H 2 accumulation was quantified. B. A time course of H 2 accumulation. P. atrosepticum SCRI1043 was incubated in M9 medium supplemented with 0.8% (w/v) glucose at 24°C and gaseous H 2 accumulation was measured every 24 h. C. P. atrosepticum SCRI1043 was incubated in M9 medium supplemented with either 0.5% (v/v) glycerol and 0.4% (w/v) nitrate (‘Gly Nit’); 0.5% (v/v) glycerol and 0.4% (w/v) fumarate (‘Gly Fum’); 0.5% (v/v) glycerol only (Gly); or 0.8% (w/v) glucose only (‘Glc’) at 24°C for 48 h. In all cases, the levels of molecular H 2 in the culture headspace were quantified by GC and normalised to OD 600 and culture volume. Error bars represent SD ( n = 3).
    Figure Legend Snippet: P. atrosepticum produces molecular hydrogen gas. A. Anaerobic hydrogen production is optimal at lower temperatures. The P. atrosepticum SCRI1043 parent strain was incubated in M9 medium supplemented with 0.8% (w/v) glucose for 168 h at the temperatures indicated before gaseous H 2 accumulation was quantified. B. A time course of H 2 accumulation. P. atrosepticum SCRI1043 was incubated in M9 medium supplemented with 0.8% (w/v) glucose at 24°C and gaseous H 2 accumulation was measured every 24 h. C. P. atrosepticum SCRI1043 was incubated in M9 medium supplemented with either 0.5% (v/v) glycerol and 0.4% (w/v) nitrate (‘Gly Nit’); 0.5% (v/v) glycerol and 0.4% (w/v) fumarate (‘Gly Fum’); 0.5% (v/v) glycerol only (Gly); or 0.8% (w/v) glucose only (‘Glc’) at 24°C for 48 h. In all cases, the levels of molecular H 2 in the culture headspace were quantified by GC and normalised to OD 600 and culture volume. Error bars represent SD ( n = 3).

    Techniques Used: Incubation

    Hydrogen gas is produced by the activity of a selenium‐free formate dehydrogenase. A. Addition of exogenous formate increases H 2 production. P. atrosepticum parental strain SCRI1043 and mutants PH001 (Δ hyfG ) and PH002 (Δ hybC ) were incubated in low‐salt (5 g l –1 ) LB (LSLB) rich medium supplemented with 0.2% or 0.4% (w/v) formate at 24°C for 48 h. B. The formate dehydrogenase encoded within the gene cluster is responsible for FHL‐2 activity. Strains SCRI1043, PH004 (Δ fdhF ), PH005 (Δ hybC Δ fdhF ) were incubated in M9 medium supplemented with 0.8% (w/v) glucose at 24°C for 48 h. C. Alternative formate dehydrogenase homologues do not have a major role in H 2 production. Strains SCRI1043, PH002 (Δ hybC ), PH019 (Δ hybC Δ ECA1964 ), PH028 (Δ hybC Δ ECA1507 ) and PH005 (Δ hybC Δ fdhF ) were incubated in M9 medium supplemented with 0.8% (w/v) glucose at 24°C for 48 h. D. Complementation of the mutant phenotype in trans . Strains PH002 (Δ hybC ) and PH005 (Δ hybC Δ fdhF ) were separately transformed with plasmids encoding either FdhF, ECA1964 or ECA1507 under the control of constitutive promoters. In all cases, the levels of molecular H 2 in the culture headspace were quantified by GC and normalised to OD 600 and culture volume. Error bars represent SD ( n = 3). In panel (D) a one‐tailed t ‐test was used to determine statistical significance (* P
    Figure Legend Snippet: Hydrogen gas is produced by the activity of a selenium‐free formate dehydrogenase. A. Addition of exogenous formate increases H 2 production. P. atrosepticum parental strain SCRI1043 and mutants PH001 (Δ hyfG ) and PH002 (Δ hybC ) were incubated in low‐salt (5 g l –1 ) LB (LSLB) rich medium supplemented with 0.2% or 0.4% (w/v) formate at 24°C for 48 h. B. The formate dehydrogenase encoded within the gene cluster is responsible for FHL‐2 activity. Strains SCRI1043, PH004 (Δ fdhF ), PH005 (Δ hybC Δ fdhF ) were incubated in M9 medium supplemented with 0.8% (w/v) glucose at 24°C for 48 h. C. Alternative formate dehydrogenase homologues do not have a major role in H 2 production. Strains SCRI1043, PH002 (Δ hybC ), PH019 (Δ hybC Δ ECA1964 ), PH028 (Δ hybC Δ ECA1507 ) and PH005 (Δ hybC Δ fdhF ) were incubated in M9 medium supplemented with 0.8% (w/v) glucose at 24°C for 48 h. D. Complementation of the mutant phenotype in trans . Strains PH002 (Δ hybC ) and PH005 (Δ hybC Δ fdhF ) were separately transformed with plasmids encoding either FdhF, ECA1964 or ECA1507 under the control of constitutive promoters. In all cases, the levels of molecular H 2 in the culture headspace were quantified by GC and normalised to OD 600 and culture volume. Error bars represent SD ( n = 3). In panel (D) a one‐tailed t ‐test was used to determine statistical significance (* P

    Techniques Used: Produced, Activity Assay, Incubation, Mutagenesis, Transformation Assay, One-tailed Test

    Hydrogen gas is produced by the activity of [NiFe]‐Hydrogenase‐4. A. Hyd‐4 is responsible for fermentative H 2 production. P. atrosepticum parental strain SCRI1043 and mutants PH001 (Δ hyfG ), PH002 (Δ hybC ) and PH003 (Δ hybC Δ hyfG ) were incubated in M9 medium supplemented with 0.8% (w/v) glucose at 24°C for 48 h. B. Complementation of the mutant phenotype in trans . Strains PH001 (Δ hyfG ), PH002 (Δ hybC ) and PH003 (Δ hybC Δ hyfG ) were separately transformed with plasmids encoding either HyfG or HybC under the control of constitutive promoters. Levels of molecular H 2 in the culture headspace were quantified by GC and normalised to OD 600 and culture volume. Error bars represent SD ( n = 3).
    Figure Legend Snippet: Hydrogen gas is produced by the activity of [NiFe]‐Hydrogenase‐4. A. Hyd‐4 is responsible for fermentative H 2 production. P. atrosepticum parental strain SCRI1043 and mutants PH001 (Δ hyfG ), PH002 (Δ hybC ) and PH003 (Δ hybC Δ hyfG ) were incubated in M9 medium supplemented with 0.8% (w/v) glucose at 24°C for 48 h. B. Complementation of the mutant phenotype in trans . Strains PH001 (Δ hyfG ), PH002 (Δ hybC ) and PH003 (Δ hybC Δ hyfG ) were separately transformed with plasmids encoding either HyfG or HybC under the control of constitutive promoters. Levels of molecular H 2 in the culture headspace were quantified by GC and normalised to OD 600 and culture volume. Error bars represent SD ( n = 3).

    Techniques Used: Produced, Activity Assay, Incubation, Mutagenesis, Transformation Assay

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    Article Snippet: The universal primers used to amplify the test-oligonucleotide pool also served as the flanking homology regions for the NEBuilder® assembly. .. Two μL of the NEBuilder® assembly product were transformed into 60 μL chemically competent E. coli (transformation efficiency > 5 × 108 cfu/μg) (Takara, Mountain View, CA, United States) and plated on six standard 100 mm LB-agar plates containing 100 μg/ml ampicillin. .. After overnight incubation, all colonies were dislodged from the plates by adding 2 ml LB-broth containing 100 μg/ml ampicillin and agitation using 10–20 ColiRollersTM glass beads (EMD Millipore, Billerica, MA, United States).

    Clone Assay:

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    Plasmid Preparation:

    Article Title: Mosaic Ends Tagmentation (METa) assembly for extremely efficient construction of functional metagenomic libraries
    Article Snippet: .. NEBuilder HiFi assembly was performed as before, with triplicate reactions containing 40 ng of inserts and 20 ng of pZE21-ME linear vector, and parallel triplicate sham reactions containing milliQ water in place of inserts. .. Blunt ligation reactions were prepared to follow established functional metagenomic library cloning protocols ( , ).

    Amplification:

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    Polymerase Chain Reaction:

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    New England Biolabs p atrosepticum scri1043 genomic dna
    P. <t>atrosepticum</t> produces molecular hydrogen gas. A. Anaerobic hydrogen production is optimal at lower temperatures. The P. atrosepticum SCRI1043 parent strain was incubated in M9 medium supplemented with 0.8% (w/v) glucose for 168 h at the temperatures indicated before gaseous H 2 accumulation was quantified. B. A time course of H 2 accumulation. P. atrosepticum SCRI1043 was incubated in M9 medium supplemented with 0.8% (w/v) glucose at 24°C and gaseous H 2 accumulation was measured every 24 h. C. P. atrosepticum SCRI1043 was incubated in M9 medium supplemented with either 0.5% (v/v) glycerol and 0.4% (w/v) nitrate (‘Gly Nit’); 0.5% (v/v) glycerol and 0.4% (w/v) fumarate (‘Gly Fum’); 0.5% (v/v) glycerol only (Gly); or 0.8% (w/v) glucose only (‘Glc’) at 24°C for 48 h. In all cases, the levels of molecular H 2 in the culture headspace were quantified by GC and normalised to OD 600 and culture volume. Error bars represent SD ( n = 3).
    P Atrosepticum Scri1043 Genomic Dna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    P. atrosepticum produces molecular hydrogen gas. A. Anaerobic hydrogen production is optimal at lower temperatures. The P. atrosepticum SCRI1043 parent strain was incubated in M9 medium supplemented with 0.8% (w/v) glucose for 168 h at the temperatures indicated before gaseous H 2 accumulation was quantified. B. A time course of H 2 accumulation. P. atrosepticum SCRI1043 was incubated in M9 medium supplemented with 0.8% (w/v) glucose at 24°C and gaseous H 2 accumulation was measured every 24 h. C. P. atrosepticum SCRI1043 was incubated in M9 medium supplemented with either 0.5% (v/v) glycerol and 0.4% (w/v) nitrate (‘Gly Nit’); 0.5% (v/v) glycerol and 0.4% (w/v) fumarate (‘Gly Fum’); 0.5% (v/v) glycerol only (Gly); or 0.8% (w/v) glucose only (‘Glc’) at 24°C for 48 h. In all cases, the levels of molecular H 2 in the culture headspace were quantified by GC and normalised to OD 600 and culture volume. Error bars represent SD ( n = 3).

    Journal: Molecular Microbiology

    Article Title: The plant pathogen Pectobacterium atrosepticum contains a functional formate hydrogenlyase‐2 complex

    doi: 10.1111/mmi.14370

    Figure Lengend Snippet: P. atrosepticum produces molecular hydrogen gas. A. Anaerobic hydrogen production is optimal at lower temperatures. The P. atrosepticum SCRI1043 parent strain was incubated in M9 medium supplemented with 0.8% (w/v) glucose for 168 h at the temperatures indicated before gaseous H 2 accumulation was quantified. B. A time course of H 2 accumulation. P. atrosepticum SCRI1043 was incubated in M9 medium supplemented with 0.8% (w/v) glucose at 24°C and gaseous H 2 accumulation was measured every 24 h. C. P. atrosepticum SCRI1043 was incubated in M9 medium supplemented with either 0.5% (v/v) glycerol and 0.4% (w/v) nitrate (‘Gly Nit’); 0.5% (v/v) glycerol and 0.4% (w/v) fumarate (‘Gly Fum’); 0.5% (v/v) glycerol only (Gly); or 0.8% (w/v) glucose only (‘Glc’) at 24°C for 48 h. In all cases, the levels of molecular H 2 in the culture headspace were quantified by GC and normalised to OD 600 and culture volume. Error bars represent SD ( n = 3).

    Article Snippet: Plasmids and complementation All plasmids were cloned using Gibson assembly (HiFi Assembly, NEB) using DNA amplified from P. atrosepticum SCRI1043 genomic DNA (Table ).

    Techniques: Incubation

    Hydrogen gas is produced by the activity of a selenium‐free formate dehydrogenase. A. Addition of exogenous formate increases H 2 production. P. atrosepticum parental strain SCRI1043 and mutants PH001 (Δ hyfG ) and PH002 (Δ hybC ) were incubated in low‐salt (5 g l –1 ) LB (LSLB) rich medium supplemented with 0.2% or 0.4% (w/v) formate at 24°C for 48 h. B. The formate dehydrogenase encoded within the gene cluster is responsible for FHL‐2 activity. Strains SCRI1043, PH004 (Δ fdhF ), PH005 (Δ hybC Δ fdhF ) were incubated in M9 medium supplemented with 0.8% (w/v) glucose at 24°C for 48 h. C. Alternative formate dehydrogenase homologues do not have a major role in H 2 production. Strains SCRI1043, PH002 (Δ hybC ), PH019 (Δ hybC Δ ECA1964 ), PH028 (Δ hybC Δ ECA1507 ) and PH005 (Δ hybC Δ fdhF ) were incubated in M9 medium supplemented with 0.8% (w/v) glucose at 24°C for 48 h. D. Complementation of the mutant phenotype in trans . Strains PH002 (Δ hybC ) and PH005 (Δ hybC Δ fdhF ) were separately transformed with plasmids encoding either FdhF, ECA1964 or ECA1507 under the control of constitutive promoters. In all cases, the levels of molecular H 2 in the culture headspace were quantified by GC and normalised to OD 600 and culture volume. Error bars represent SD ( n = 3). In panel (D) a one‐tailed t ‐test was used to determine statistical significance (* P

    Journal: Molecular Microbiology

    Article Title: The plant pathogen Pectobacterium atrosepticum contains a functional formate hydrogenlyase‐2 complex

    doi: 10.1111/mmi.14370

    Figure Lengend Snippet: Hydrogen gas is produced by the activity of a selenium‐free formate dehydrogenase. A. Addition of exogenous formate increases H 2 production. P. atrosepticum parental strain SCRI1043 and mutants PH001 (Δ hyfG ) and PH002 (Δ hybC ) were incubated in low‐salt (5 g l –1 ) LB (LSLB) rich medium supplemented with 0.2% or 0.4% (w/v) formate at 24°C for 48 h. B. The formate dehydrogenase encoded within the gene cluster is responsible for FHL‐2 activity. Strains SCRI1043, PH004 (Δ fdhF ), PH005 (Δ hybC Δ fdhF ) were incubated in M9 medium supplemented with 0.8% (w/v) glucose at 24°C for 48 h. C. Alternative formate dehydrogenase homologues do not have a major role in H 2 production. Strains SCRI1043, PH002 (Δ hybC ), PH019 (Δ hybC Δ ECA1964 ), PH028 (Δ hybC Δ ECA1507 ) and PH005 (Δ hybC Δ fdhF ) were incubated in M9 medium supplemented with 0.8% (w/v) glucose at 24°C for 48 h. D. Complementation of the mutant phenotype in trans . Strains PH002 (Δ hybC ) and PH005 (Δ hybC Δ fdhF ) were separately transformed with plasmids encoding either FdhF, ECA1964 or ECA1507 under the control of constitutive promoters. In all cases, the levels of molecular H 2 in the culture headspace were quantified by GC and normalised to OD 600 and culture volume. Error bars represent SD ( n = 3). In panel (D) a one‐tailed t ‐test was used to determine statistical significance (* P

    Article Snippet: Plasmids and complementation All plasmids were cloned using Gibson assembly (HiFi Assembly, NEB) using DNA amplified from P. atrosepticum SCRI1043 genomic DNA (Table ).

    Techniques: Produced, Activity Assay, Incubation, Mutagenesis, Transformation Assay, One-tailed Test

    Hydrogen gas is produced by the activity of [NiFe]‐Hydrogenase‐4. A. Hyd‐4 is responsible for fermentative H 2 production. P. atrosepticum parental strain SCRI1043 and mutants PH001 (Δ hyfG ), PH002 (Δ hybC ) and PH003 (Δ hybC Δ hyfG ) were incubated in M9 medium supplemented with 0.8% (w/v) glucose at 24°C for 48 h. B. Complementation of the mutant phenotype in trans . Strains PH001 (Δ hyfG ), PH002 (Δ hybC ) and PH003 (Δ hybC Δ hyfG ) were separately transformed with plasmids encoding either HyfG or HybC under the control of constitutive promoters. Levels of molecular H 2 in the culture headspace were quantified by GC and normalised to OD 600 and culture volume. Error bars represent SD ( n = 3).

    Journal: Molecular Microbiology

    Article Title: The plant pathogen Pectobacterium atrosepticum contains a functional formate hydrogenlyase‐2 complex

    doi: 10.1111/mmi.14370

    Figure Lengend Snippet: Hydrogen gas is produced by the activity of [NiFe]‐Hydrogenase‐4. A. Hyd‐4 is responsible for fermentative H 2 production. P. atrosepticum parental strain SCRI1043 and mutants PH001 (Δ hyfG ), PH002 (Δ hybC ) and PH003 (Δ hybC Δ hyfG ) were incubated in M9 medium supplemented with 0.8% (w/v) glucose at 24°C for 48 h. B. Complementation of the mutant phenotype in trans . Strains PH001 (Δ hyfG ), PH002 (Δ hybC ) and PH003 (Δ hybC Δ hyfG ) were separately transformed with plasmids encoding either HyfG or HybC under the control of constitutive promoters. Levels of molecular H 2 in the culture headspace were quantified by GC and normalised to OD 600 and culture volume. Error bars represent SD ( n = 3).

    Article Snippet: Plasmids and complementation All plasmids were cloned using Gibson assembly (HiFi Assembly, NEB) using DNA amplified from P. atrosepticum SCRI1043 genomic DNA (Table ).

    Techniques: Produced, Activity Assay, Incubation, Mutagenesis, Transformation Assay