Journal: bioRxiv

Article Title: Insulin and Exercise-induced Phosphoproteomics of Human Skeletal Muscle Identify REPS1 as a New Regulator of Muscle Glucose Uptake

doi: 10.1101/2023.11.10.566644

Figure Lengend Snippet: Quantified western blot analysis of p-mTOR S2448 (A), p-AKT S473 (B), p-AMPK T172 (C) and p-ACC S221 (D) in human skeletal muscle pre/post an hyperinsulinemic euglycemic clamp (2hr) and pre/post 10 minutes of high intensity cycling exercise. Paired-Samples t-test was used to analyze mean differences within each experiment. P < 0.05 = *, P < 0.01 = **, P < 0.001 = ***.

Article Snippet: REPS1 (CST #6404), p-REPS S709 (CST #12005), GAPDH (CST #2118), p-CREB S133 (CST #9198), p-AKT T308 (CST #9275), p-AKT S473 (CST #9271), AKT2 (CST #3063), pan-AKT total (CST #9272), p-ERK1/2 T202/Y204 (CST #9101), ERK1/2 (CST #4696), mTOR (CST #2972), p-mTOR S2448 (CST #2971), p-AMPK T172(CST #2531), AMPKα2 (CST #2532), ACC (CST #3676), p-P70S6K T389 (CST# 9205), P70S6K (CST #2708), p-RPS6 S235/236 (CST #2211), RPS6 (CST #2217), ACTIN (sigma A2066), p-ACC S221 (Millipore 07-303), GLUT4 (Thermo PA1-1065), FLAG (sigma, F7425).

Techniques: Western Blot