p akt the308  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc p akt the308
    P Akt The308, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    p akt the308  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc p akt the308
    P Akt The308, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Proteintech rabbit anti p akt
    Antibody information.
    Rabbit Anti P Akt, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Anti‑epileptic mechanism of isopimaric acid from Platycladi cacumen based on network pharmacology, molecular docking and biological validation"

    Article Title: Anti‑epileptic mechanism of isopimaric acid from Platycladi cacumen based on network pharmacology, molecular docking and biological validation

    Journal: Experimental and Therapeutic Medicine

    doi: 10.3892/etm.2024.12637

    Antibody information.
    Figure Legend Snippet: Antibody information.

    Techniques Used:

    anti-p-akt  (Danaher Inc)


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    Danaher Inc anti-p-akt
    Anti P Akt, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti p akt cat no 4060  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti p akt cat no 4060
    Anti P Akt Cat No 4060, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit anti phosphorylated akt p akt  (Danaher Inc)


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    Danaher Inc rabbit anti phosphorylated akt p akt
    Expression of <t>phosphorylated-AKT</t> (p-AKT), AKT, phosphorylated-GSK3β (p-GSK3β), GSK3β, and β-catenin in the nucleus of the ipsilateral hemisphere (excluding the infarct area). (A) Western blot analysis of p-AKT, p-GSK3β, and β-catenin in the nucleus. Protein expression levels were normalized to GAPDH (total protein) or H3 (nuclear protein) ( n = 3). Expression of nuclear β-catenin, p-AKT, and p-GSK3β were higher in rats of the TMS (+) group compared with the TMS (–) group at 3, 7, 14, and 28 days. (B) Immunofluorescence of nuclear β-catenin (red, Alexa Fluor® 555) and Nestin (green)-positive cells in the TMS (+), TMS (–), and Sham groups ( n = 5). Nuclear β-catenin was upregulated in the TMS (+) group (analysis of variance [ANOVA]). Scale bars: 25 μm. (C) Ki67 (red, Alexa Fluor® 555) and Nestin (green, Alexa Fluor® 488)-positive cells in the TMS (–), TMS (H), and TMS + LY groups ( n = 5). More Ki67 + /Nestin + cells were detected in the TMS (H) group. Scale bars: 25 μm. (D) CCK8 analysis. Cell viability was decreased in the TMS (H) + LY group ( n = 5). (E) Western blot analysis of p-AKT, p-GSK3β, and β-catenin in the nucleus. GAPDH (total protein) or H3 (nuclear protein) were used as the internal control. Data are shown as mean ± SD. * P < 0.05, ** P < 0.01 (A: Student's t -test; B, D, E: Brown-Forsythe and Welch ANOVA tests followed by Dunnett's T3 multiple comparison test). D3/7/14/28 TMS (–): rats with no TMS at 3/7/14/28 days group; D3/7/14/28 TMS (+): rats with 10 Hz TMS at 3/7/14/28 days group; GAPDH: glyceric acid phosphated hydrogenase; ns: no significant; NSC: neural stem cell; SHAM: sham-operation group; TMS: transcranial magnetic stimulation; TMS (–): no TMS group; TMS (+): high-frequency TMS with 10 Hz group; TMS(H): NSCs with 10 Hz high-frequency TMS group; TMS(H) + LY: NSCs with 10 Hz high-frequency TMS and LY294002 group.
    Rabbit Anti Phosphorylated Akt P Akt, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "High-frequency repetitive transcranial magnetic stimulation promotes neural stem cell proliferation after ischemic stroke"

    Article Title: High-frequency repetitive transcranial magnetic stimulation promotes neural stem cell proliferation after ischemic stroke

    Journal: Neural Regeneration Research

    doi: 10.4103/1673-5374.389303

    Expression of phosphorylated-AKT (p-AKT), AKT, phosphorylated-GSK3β (p-GSK3β), GSK3β, and β-catenin in the nucleus of the ipsilateral hemisphere (excluding the infarct area). (A) Western blot analysis of p-AKT, p-GSK3β, and β-catenin in the nucleus. Protein expression levels were normalized to GAPDH (total protein) or H3 (nuclear protein) ( n = 3). Expression of nuclear β-catenin, p-AKT, and p-GSK3β were higher in rats of the TMS (+) group compared with the TMS (–) group at 3, 7, 14, and 28 days. (B) Immunofluorescence of nuclear β-catenin (red, Alexa Fluor® 555) and Nestin (green)-positive cells in the TMS (+), TMS (–), and Sham groups ( n = 5). Nuclear β-catenin was upregulated in the TMS (+) group (analysis of variance [ANOVA]). Scale bars: 25 μm. (C) Ki67 (red, Alexa Fluor® 555) and Nestin (green, Alexa Fluor® 488)-positive cells in the TMS (–), TMS (H), and TMS + LY groups ( n = 5). More Ki67 + /Nestin + cells were detected in the TMS (H) group. Scale bars: 25 μm. (D) CCK8 analysis. Cell viability was decreased in the TMS (H) + LY group ( n = 5). (E) Western blot analysis of p-AKT, p-GSK3β, and β-catenin in the nucleus. GAPDH (total protein) or H3 (nuclear protein) were used as the internal control. Data are shown as mean ± SD. * P < 0.05, ** P < 0.01 (A: Student's t -test; B, D, E: Brown-Forsythe and Welch ANOVA tests followed by Dunnett's T3 multiple comparison test). D3/7/14/28 TMS (–): rats with no TMS at 3/7/14/28 days group; D3/7/14/28 TMS (+): rats with 10 Hz TMS at 3/7/14/28 days group; GAPDH: glyceric acid phosphated hydrogenase; ns: no significant; NSC: neural stem cell; SHAM: sham-operation group; TMS: transcranial magnetic stimulation; TMS (–): no TMS group; TMS (+): high-frequency TMS with 10 Hz group; TMS(H): NSCs with 10 Hz high-frequency TMS group; TMS(H) + LY: NSCs with 10 Hz high-frequency TMS and LY294002 group.
    Figure Legend Snippet: Expression of phosphorylated-AKT (p-AKT), AKT, phosphorylated-GSK3β (p-GSK3β), GSK3β, and β-catenin in the nucleus of the ipsilateral hemisphere (excluding the infarct area). (A) Western blot analysis of p-AKT, p-GSK3β, and β-catenin in the nucleus. Protein expression levels were normalized to GAPDH (total protein) or H3 (nuclear protein) ( n = 3). Expression of nuclear β-catenin, p-AKT, and p-GSK3β were higher in rats of the TMS (+) group compared with the TMS (–) group at 3, 7, 14, and 28 days. (B) Immunofluorescence of nuclear β-catenin (red, Alexa Fluor® 555) and Nestin (green)-positive cells in the TMS (+), TMS (–), and Sham groups ( n = 5). Nuclear β-catenin was upregulated in the TMS (+) group (analysis of variance [ANOVA]). Scale bars: 25 μm. (C) Ki67 (red, Alexa Fluor® 555) and Nestin (green, Alexa Fluor® 488)-positive cells in the TMS (–), TMS (H), and TMS + LY groups ( n = 5). More Ki67 + /Nestin + cells were detected in the TMS (H) group. Scale bars: 25 μm. (D) CCK8 analysis. Cell viability was decreased in the TMS (H) + LY group ( n = 5). (E) Western blot analysis of p-AKT, p-GSK3β, and β-catenin in the nucleus. GAPDH (total protein) or H3 (nuclear protein) were used as the internal control. Data are shown as mean ± SD. * P < 0.05, ** P < 0.01 (A: Student's t -test; B, D, E: Brown-Forsythe and Welch ANOVA tests followed by Dunnett's T3 multiple comparison test). D3/7/14/28 TMS (–): rats with no TMS at 3/7/14/28 days group; D3/7/14/28 TMS (+): rats with 10 Hz TMS at 3/7/14/28 days group; GAPDH: glyceric acid phosphated hydrogenase; ns: no significant; NSC: neural stem cell; SHAM: sham-operation group; TMS: transcranial magnetic stimulation; TMS (–): no TMS group; TMS (+): high-frequency TMS with 10 Hz group; TMS(H): NSCs with 10 Hz high-frequency TMS group; TMS(H) + LY: NSCs with 10 Hz high-frequency TMS and LY294002 group.

    Techniques Used: Expressing, Western Blot, Immunofluorescence, Comparison

    rTMS activates AKT through the Ca 2+ /P2 calcium channel/CaM pathway. (A) Calcium imaging of NSCs by Fluo-4 AM labeling. Cells in the TMS (+) group showed stronger signal. Scale bars: 25 μm. (B, C) Levels of Ca 2+ influx by fluorescence intensity (B; n = 5) and flow cytometry (C; n = 4). (D) Western blot analysis of p-AKT, p-GSK3β, and β-catenin in the nucleus. GAPDH (total protein) or H3 (nuclear protein) were used as the internal control. Expression of nuclear β-catenin, p-GSK3β, and p-AKT were higher in NSCs of the TMS (H) group compared with other groups. Data are shown as mean ± SD. * P < 0.05, ** P < 0.01 (B: Student's t -test; D: Brown-Forsythe and Welch analysis of variance tests followed by Dunnett's T3 multiple comparison test). 2-APB: 2-Aminoethyl diphenylborinate; CPZ: chlorpromazine; CTRL: NSCs with no OGD group; NSC: neural stem cell; OGD: NSCs with OGD group; p-AKT: phosphorylated-AKT; p-GSK3β: phosphorylated-glycogen synthase kinase-3β; TMS(H): NSCs with 10 Hz high-frequency TMS group; U7: inhibitor U73122.
    Figure Legend Snippet: rTMS activates AKT through the Ca 2+ /P2 calcium channel/CaM pathway. (A) Calcium imaging of NSCs by Fluo-4 AM labeling. Cells in the TMS (+) group showed stronger signal. Scale bars: 25 μm. (B, C) Levels of Ca 2+ influx by fluorescence intensity (B; n = 5) and flow cytometry (C; n = 4). (D) Western blot analysis of p-AKT, p-GSK3β, and β-catenin in the nucleus. GAPDH (total protein) or H3 (nuclear protein) were used as the internal control. Expression of nuclear β-catenin, p-GSK3β, and p-AKT were higher in NSCs of the TMS (H) group compared with other groups. Data are shown as mean ± SD. * P < 0.05, ** P < 0.01 (B: Student's t -test; D: Brown-Forsythe and Welch analysis of variance tests followed by Dunnett's T3 multiple comparison test). 2-APB: 2-Aminoethyl diphenylborinate; CPZ: chlorpromazine; CTRL: NSCs with no OGD group; NSC: neural stem cell; OGD: NSCs with OGD group; p-AKT: phosphorylated-AKT; p-GSK3β: phosphorylated-glycogen synthase kinase-3β; TMS(H): NSCs with 10 Hz high-frequency TMS group; U7: inhibitor U73122.

    Techniques Used: Imaging, Labeling, Fluorescence, Flow Cytometry, Western Blot, Expressing, Comparison

    rabbit anti phosphorylated akt p akt  (Danaher Inc)


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    Danaher Inc rabbit anti phosphorylated akt p akt
    Rabbit Anti Phosphorylated Akt P Akt, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti phospho protein kinase b p akt antibody  (Danaher Inc)


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    Danaher Inc anti phospho protein kinase b p akt antibody
    Anti Phospho Protein Kinase B P Akt Antibody, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti phospho protein kinase b p akt antibody  (Danaher Inc)


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    Danaher Inc anti phospho protein kinase b p akt antibody
    Anti Phospho Protein Kinase B P Akt Antibody, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    α sma col ⅰ p smad3 smad3 p pan akt pan akt p pi3k p85 pi3k p85 p mtor mtor lc3ⅰ ⅱ sqstm1 p62 tubulin gapdh  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc α sma col ⅰ p smad3 smad3 p pan akt pan akt p pi3k p85 pi3k p85 p mtor mtor lc3ⅰ ⅱ sqstm1 p62 tubulin gapdh
    Specific primary antibodies in Western blot.
    α Sma Col ⅰ P Smad3 Smad3 P Pan Akt Pan Akt P Pi3k P85 Pi3k P85 P Mtor Mtor Lc3ⅰ ⅱ Sqstm1 P62 Tubulin Gapdh, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    α sma col ⅰ p smad3 smad3 p pan akt pan akt p pi3k p85 pi3k p85 p mtor mtor lc3ⅰ ⅱ sqstm1 p62 tubulin gapdh - by Bioz Stars, 2024-07
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    1) Product Images from "Remdesivir alleviates skin fibrosis by suppressing TGF-β1 signaling pathway"

    Article Title: Remdesivir alleviates skin fibrosis by suppressing TGF-β1 signaling pathway

    Journal: PLOS ONE

    doi: 10.1371/journal.pone.0305927

    Specific primary antibodies in Western blot.
    Figure Legend Snippet: Specific primary antibodies in Western blot.

    Techniques Used: Western Blot

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    Cell Signaling Technology Inc p akt the308
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    Expression of <t>phosphorylated-AKT</t> (p-AKT), AKT, phosphorylated-GSK3β (p-GSK3β), GSK3β, and β-catenin in the nucleus of the ipsilateral hemisphere (excluding the infarct area). (A) Western blot analysis of p-AKT, p-GSK3β, and β-catenin in the nucleus. Protein expression levels were normalized to GAPDH (total protein) or H3 (nuclear protein) ( n = 3). Expression of nuclear β-catenin, p-AKT, and p-GSK3β were higher in rats of the TMS (+) group compared with the TMS (–) group at 3, 7, 14, and 28 days. (B) Immunofluorescence of nuclear β-catenin (red, Alexa Fluor® 555) and Nestin (green)-positive cells in the TMS (+), TMS (–), and Sham groups ( n = 5). Nuclear β-catenin was upregulated in the TMS (+) group (analysis of variance [ANOVA]). Scale bars: 25 μm. (C) Ki67 (red, Alexa Fluor® 555) and Nestin (green, Alexa Fluor® 488)-positive cells in the TMS (–), TMS (H), and TMS + LY groups ( n = 5). More Ki67 + /Nestin + cells were detected in the TMS (H) group. Scale bars: 25 μm. (D) CCK8 analysis. Cell viability was decreased in the TMS (H) + LY group ( n = 5). (E) Western blot analysis of p-AKT, p-GSK3β, and β-catenin in the nucleus. GAPDH (total protein) or H3 (nuclear protein) were used as the internal control. Data are shown as mean ± SD. * P < 0.05, ** P < 0.01 (A: Student's t -test; B, D, E: Brown-Forsythe and Welch ANOVA tests followed by Dunnett's T3 multiple comparison test). D3/7/14/28 TMS (–): rats with no TMS at 3/7/14/28 days group; D3/7/14/28 TMS (+): rats with 10 Hz TMS at 3/7/14/28 days group; GAPDH: glyceric acid phosphated hydrogenase; ns: no significant; NSC: neural stem cell; SHAM: sham-operation group; TMS: transcranial magnetic stimulation; TMS (–): no TMS group; TMS (+): high-frequency TMS with 10 Hz group; TMS(H): NSCs with 10 Hz high-frequency TMS group; TMS(H) + LY: NSCs with 10 Hz high-frequency TMS and LY294002 group.
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    Anti Phospho Protein Kinase B P Akt Antibody, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc α sma col ⅰ p smad3 smad3 p pan akt pan akt p pi3k p85 pi3k p85 p mtor mtor lc3ⅰ ⅱ sqstm1 p62 tubulin gapdh
    Specific primary antibodies in Western blot.
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    Antibody information.

    Journal: Experimental and Therapeutic Medicine

    Article Title: Anti‑epileptic mechanism of isopimaric acid from Platycladi cacumen based on network pharmacology, molecular docking and biological validation

    doi: 10.3892/etm.2024.12637

    Figure Lengend Snippet: Antibody information.

    Article Snippet: Rabbit anti-p-AKT , Proteintech Group, Inc. , 80455-1-RR , 1:5,000.

    Techniques:

    Expression of phosphorylated-AKT (p-AKT), AKT, phosphorylated-GSK3β (p-GSK3β), GSK3β, and β-catenin in the nucleus of the ipsilateral hemisphere (excluding the infarct area). (A) Western blot analysis of p-AKT, p-GSK3β, and β-catenin in the nucleus. Protein expression levels were normalized to GAPDH (total protein) or H3 (nuclear protein) ( n = 3). Expression of nuclear β-catenin, p-AKT, and p-GSK3β were higher in rats of the TMS (+) group compared with the TMS (–) group at 3, 7, 14, and 28 days. (B) Immunofluorescence of nuclear β-catenin (red, Alexa Fluor® 555) and Nestin (green)-positive cells in the TMS (+), TMS (–), and Sham groups ( n = 5). Nuclear β-catenin was upregulated in the TMS (+) group (analysis of variance [ANOVA]). Scale bars: 25 μm. (C) Ki67 (red, Alexa Fluor® 555) and Nestin (green, Alexa Fluor® 488)-positive cells in the TMS (–), TMS (H), and TMS + LY groups ( n = 5). More Ki67 + /Nestin + cells were detected in the TMS (H) group. Scale bars: 25 μm. (D) CCK8 analysis. Cell viability was decreased in the TMS (H) + LY group ( n = 5). (E) Western blot analysis of p-AKT, p-GSK3β, and β-catenin in the nucleus. GAPDH (total protein) or H3 (nuclear protein) were used as the internal control. Data are shown as mean ± SD. * P < 0.05, ** P < 0.01 (A: Student's t -test; B, D, E: Brown-Forsythe and Welch ANOVA tests followed by Dunnett's T3 multiple comparison test). D3/7/14/28 TMS (–): rats with no TMS at 3/7/14/28 days group; D3/7/14/28 TMS (+): rats with 10 Hz TMS at 3/7/14/28 days group; GAPDH: glyceric acid phosphated hydrogenase; ns: no significant; NSC: neural stem cell; SHAM: sham-operation group; TMS: transcranial magnetic stimulation; TMS (–): no TMS group; TMS (+): high-frequency TMS with 10 Hz group; TMS(H): NSCs with 10 Hz high-frequency TMS group; TMS(H) + LY: NSCs with 10 Hz high-frequency TMS and LY294002 group.

    Journal: Neural Regeneration Research

    Article Title: High-frequency repetitive transcranial magnetic stimulation promotes neural stem cell proliferation after ischemic stroke

    doi: 10.4103/1673-5374.389303

    Figure Lengend Snippet: Expression of phosphorylated-AKT (p-AKT), AKT, phosphorylated-GSK3β (p-GSK3β), GSK3β, and β-catenin in the nucleus of the ipsilateral hemisphere (excluding the infarct area). (A) Western blot analysis of p-AKT, p-GSK3β, and β-catenin in the nucleus. Protein expression levels were normalized to GAPDH (total protein) or H3 (nuclear protein) ( n = 3). Expression of nuclear β-catenin, p-AKT, and p-GSK3β were higher in rats of the TMS (+) group compared with the TMS (–) group at 3, 7, 14, and 28 days. (B) Immunofluorescence of nuclear β-catenin (red, Alexa Fluor® 555) and Nestin (green)-positive cells in the TMS (+), TMS (–), and Sham groups ( n = 5). Nuclear β-catenin was upregulated in the TMS (+) group (analysis of variance [ANOVA]). Scale bars: 25 μm. (C) Ki67 (red, Alexa Fluor® 555) and Nestin (green, Alexa Fluor® 488)-positive cells in the TMS (–), TMS (H), and TMS + LY groups ( n = 5). More Ki67 + /Nestin + cells were detected in the TMS (H) group. Scale bars: 25 μm. (D) CCK8 analysis. Cell viability was decreased in the TMS (H) + LY group ( n = 5). (E) Western blot analysis of p-AKT, p-GSK3β, and β-catenin in the nucleus. GAPDH (total protein) or H3 (nuclear protein) were used as the internal control. Data are shown as mean ± SD. * P < 0.05, ** P < 0.01 (A: Student's t -test; B, D, E: Brown-Forsythe and Welch ANOVA tests followed by Dunnett's T3 multiple comparison test). D3/7/14/28 TMS (–): rats with no TMS at 3/7/14/28 days group; D3/7/14/28 TMS (+): rats with 10 Hz TMS at 3/7/14/28 days group; GAPDH: glyceric acid phosphated hydrogenase; ns: no significant; NSC: neural stem cell; SHAM: sham-operation group; TMS: transcranial magnetic stimulation; TMS (–): no TMS group; TMS (+): high-frequency TMS with 10 Hz group; TMS(H): NSCs with 10 Hz high-frequency TMS group; TMS(H) + LY: NSCs with 10 Hz high-frequency TMS and LY294002 group.

    Article Snippet: They were then incubated overnight at 4°C with primary antibodies against: rabbit anti-β-catenin (Abcam, Cat# ab32572, RRID: AB_2629234), rabbit anti-H3 (Abcam, Cat# ab1791, RRID: AB_11184913), rabbit anti-phosphorylated-GSK3β (p-GSK3β) (phospho Ser9, Abcam, Cat# ab93926, RRID: AB_2928098), rabbit anti-GSK3β (Abcam, Cat# ab280376, RRID: AB_2928097), rabbit anti-AKT (Abcam, Cat# ab188099, RRID: AB_1662415), rabbit anti-phosphorylated-AKT (p-AKT) (phospho S473, Abcam, Cat# ab81283, RRID: AB_1662951), and rabbit anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (Abcam, Cat# ab181602, RRID: AB_1726478); all at a dilution of 1:500.

    Techniques: Expressing, Western Blot, Immunofluorescence, Comparison

    rTMS activates AKT through the Ca 2+ /P2 calcium channel/CaM pathway. (A) Calcium imaging of NSCs by Fluo-4 AM labeling. Cells in the TMS (+) group showed stronger signal. Scale bars: 25 μm. (B, C) Levels of Ca 2+ influx by fluorescence intensity (B; n = 5) and flow cytometry (C; n = 4). (D) Western blot analysis of p-AKT, p-GSK3β, and β-catenin in the nucleus. GAPDH (total protein) or H3 (nuclear protein) were used as the internal control. Expression of nuclear β-catenin, p-GSK3β, and p-AKT were higher in NSCs of the TMS (H) group compared with other groups. Data are shown as mean ± SD. * P < 0.05, ** P < 0.01 (B: Student's t -test; D: Brown-Forsythe and Welch analysis of variance tests followed by Dunnett's T3 multiple comparison test). 2-APB: 2-Aminoethyl diphenylborinate; CPZ: chlorpromazine; CTRL: NSCs with no OGD group; NSC: neural stem cell; OGD: NSCs with OGD group; p-AKT: phosphorylated-AKT; p-GSK3β: phosphorylated-glycogen synthase kinase-3β; TMS(H): NSCs with 10 Hz high-frequency TMS group; U7: inhibitor U73122.

    Journal: Neural Regeneration Research

    Article Title: High-frequency repetitive transcranial magnetic stimulation promotes neural stem cell proliferation after ischemic stroke

    doi: 10.4103/1673-5374.389303

    Figure Lengend Snippet: rTMS activates AKT through the Ca 2+ /P2 calcium channel/CaM pathway. (A) Calcium imaging of NSCs by Fluo-4 AM labeling. Cells in the TMS (+) group showed stronger signal. Scale bars: 25 μm. (B, C) Levels of Ca 2+ influx by fluorescence intensity (B; n = 5) and flow cytometry (C; n = 4). (D) Western blot analysis of p-AKT, p-GSK3β, and β-catenin in the nucleus. GAPDH (total protein) or H3 (nuclear protein) were used as the internal control. Expression of nuclear β-catenin, p-GSK3β, and p-AKT were higher in NSCs of the TMS (H) group compared with other groups. Data are shown as mean ± SD. * P < 0.05, ** P < 0.01 (B: Student's t -test; D: Brown-Forsythe and Welch analysis of variance tests followed by Dunnett's T3 multiple comparison test). 2-APB: 2-Aminoethyl diphenylborinate; CPZ: chlorpromazine; CTRL: NSCs with no OGD group; NSC: neural stem cell; OGD: NSCs with OGD group; p-AKT: phosphorylated-AKT; p-GSK3β: phosphorylated-glycogen synthase kinase-3β; TMS(H): NSCs with 10 Hz high-frequency TMS group; U7: inhibitor U73122.

    Article Snippet: They were then incubated overnight at 4°C with primary antibodies against: rabbit anti-β-catenin (Abcam, Cat# ab32572, RRID: AB_2629234), rabbit anti-H3 (Abcam, Cat# ab1791, RRID: AB_11184913), rabbit anti-phosphorylated-GSK3β (p-GSK3β) (phospho Ser9, Abcam, Cat# ab93926, RRID: AB_2928098), rabbit anti-GSK3β (Abcam, Cat# ab280376, RRID: AB_2928097), rabbit anti-AKT (Abcam, Cat# ab188099, RRID: AB_1662415), rabbit anti-phosphorylated-AKT (p-AKT) (phospho S473, Abcam, Cat# ab81283, RRID: AB_1662951), and rabbit anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (Abcam, Cat# ab181602, RRID: AB_1726478); all at a dilution of 1:500.

    Techniques: Imaging, Labeling, Fluorescence, Flow Cytometry, Western Blot, Expressing, Comparison

    Specific primary antibodies in Western blot.

    Journal: PLOS ONE

    Article Title: Remdesivir alleviates skin fibrosis by suppressing TGF-β1 signaling pathway

    doi: 10.1371/journal.pone.0305927

    Figure Lengend Snippet: Specific primary antibodies in Western blot.

    Article Snippet: α‐SMA Col-Ⅰ p‐Smad3 Smad3 p-pan-AKT pan-AKT p-PI3K p85 PI3K p85 p-mTOR mTOR LC3Ⅰ/Ⅱ SQSTM1/p62 Tubulin GAPDH , Affinity Cell Signaling Technology Cell Signaling Technology Cell Signaling Technology Affinity Affinity Affinity Affinity Cell Signaling Technology Cell Signaling Technology Cell Signaling Technology Affinity Affinity Affinity , United States United States United States United States United States United States United States United States United States United States United States United States United States United States.

    Techniques: Western Blot