p aeruginosa atcc 27853 reference strain  (ATCC)


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    Structured Review

    ATCC p aeruginosa atcc 27853 reference strain
    SEM analysis performed on P. <t>aeruginosa</t> ATCC 27853. a Untreated; b treated with AMP2041; c holes size measurement
    P Aeruginosa Atcc 27853 Reference Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Activity of AMP2041 against human and animal multidrug resistant Pseudomonas aeruginosa clinical isolates"

    Article Title: Activity of AMP2041 against human and animal multidrug resistant Pseudomonas aeruginosa clinical isolates

    Journal: Annals of Clinical Microbiology and Antimicrobials

    doi: 10.1186/s12941-017-0193-1

    SEM analysis performed on P. aeruginosa ATCC 27853. a Untreated; b treated with AMP2041; c holes size measurement
    Figure Legend Snippet: SEM analysis performed on P. aeruginosa ATCC 27853. a Untreated; b treated with AMP2041; c holes size measurement

    Techniques Used:

    2) Product Images from "Prolonged Outbreak of Infection Due to TEM-21-Producing Strains of Pseudomonas aeruginosa and Enterobacteria in a Nursing Home"

    Article Title: Prolonged Outbreak of Infection Due to TEM-21-Producing Strains of Pseudomonas aeruginosa and Enterobacteria in a Nursing Home

    Journal: Journal of Clinical Microbiology

    doi: 10.1128/JCM.43.8.4129-4138.2005

    PFGE profiles of SpeI-digested whole-cell DNA of the 24 P. aeruginosa isolates. Lane M, DNA ladder; lane control, P. aeruginosa ATCC 27853.
    Figure Legend Snippet: PFGE profiles of SpeI-digested whole-cell DNA of the 24 P. aeruginosa isolates. Lane M, DNA ladder; lane control, P. aeruginosa ATCC 27853.

    Techniques Used:

    3) Product Images from "Esculentin-1a(1-21)NH2: a frog skin-derived peptide for microbial keratitis"

    Article Title: Esculentin-1a(1-21)NH2: a frog skin-derived peptide for microbial keratitis

    Journal: Cellular and molecular life sciences : CMLS

    doi: 10.1007/s00018-014-1694-0

    Effects of NaCl on the bactericidal activity of Esc-1a(1-21)NH 2 against P. aeruginosa ATCC 27853. (a) Bacterial cells were incubated with different concentrations of Esc-1a(1-21)NH 2 in the presence of 150 mM NaCl for 20 min at 37 °C. (b) Bacterial cells were incubated with the peptide (1 μM) in the absence or in the presence of varying concentrations of NaCl at 37 °C for 20 min. The data are the mean ± standard deviation (SD) of three independent experiments and are reported as percentage of bacteria killed by the peptide compared with the corresponding control (see Materials and Methods).
    Figure Legend Snippet: Effects of NaCl on the bactericidal activity of Esc-1a(1-21)NH 2 against P. aeruginosa ATCC 27853. (a) Bacterial cells were incubated with different concentrations of Esc-1a(1-21)NH 2 in the presence of 150 mM NaCl for 20 min at 37 °C. (b) Bacterial cells were incubated with the peptide (1 μM) in the absence or in the presence of varying concentrations of NaCl at 37 °C for 20 min. The data are the mean ± standard deviation (SD) of three independent experiments and are reported as percentage of bacteria killed by the peptide compared with the corresponding control (see Materials and Methods).

    Techniques Used: Activity Assay, Incubation, Standard Deviation

    Effects of human basal tears on the bactericidal activity of Esc-1a(1-21)NH 2 against P. aeruginosa ATCC 27853 (a) and ATCC 19660 (b). Bacterial cells were incubated with the peptide in the presence of 70 % (v/v) or 50% (v/v) basal tears, as indicated, for 30, 90 and 120 min at 37 °C. Cells treated with 1 μM peptide (indicated as Esc(1-21) in the figure) in PB or with 70% (v/v) tears were included for comparison. After incubation, aliquots were withdrawn for cell counting. The data are the mean ± standard deviation (SD) of three independent experiments and are reported as percentage of bacteria killed compared with the control (see Materials and Methods). Significance was assessed using values of all groups compared with those of peptide-treated samples in the absence of tears by t -test. *-indicates significant difference, p
    Figure Legend Snippet: Effects of human basal tears on the bactericidal activity of Esc-1a(1-21)NH 2 against P. aeruginosa ATCC 27853 (a) and ATCC 19660 (b). Bacterial cells were incubated with the peptide in the presence of 70 % (v/v) or 50% (v/v) basal tears, as indicated, for 30, 90 and 120 min at 37 °C. Cells treated with 1 μM peptide (indicated as Esc(1-21) in the figure) in PB or with 70% (v/v) tears were included for comparison. After incubation, aliquots were withdrawn for cell counting. The data are the mean ± standard deviation (SD) of three independent experiments and are reported as percentage of bacteria killed compared with the control (see Materials and Methods). Significance was assessed using values of all groups compared with those of peptide-treated samples in the absence of tears by t -test. *-indicates significant difference, p

    Techniques Used: Activity Assay, Incubation, Cell Counting, Standard Deviation

    4) Product Images from "Sphingosine kills bacteria by binding to cardiolipin"

    Article Title: Sphingosine kills bacteria by binding to cardiolipin

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.RA119.012325

    Sphingosine induces a rapid increase in the permeability of P. aeruginosa . A and B, P. aeruginosa ( P.a. ) strains 762 and ATCC 27853 or S. aureus ( S.a. ) strain DH was incubated with 100 n m TO-PRO-3 iodide. Bacteria were then treated for 5 min with 1 or 10 μ m sphingosine ( SPH ) or the corresponding concentration of octylglucopyranoside ( OGP ), the solvent of sphingosine. The bacteria were analyzed by flow cytometry. A shows the quantitative analysis of the means of the fluorescence intensity obtained in the flow cytometry studies (given in arbitrary units; a.u. ), and B shows representative flow cytometry stainings from four independent experiments. Either Triton X-100 or nisin was added as a positive control for membrane permeabilization. C and D , intracellular ATP in P. aeruginosa strains 762 and ATCC 27853 or S. aureus strain DH ( C ) and the release of ATP ( D ) from the bacteria were measured with the BacTiter-Glo reagent. SPH results in a very rapid release of ATP from P. aeruginosa or S. aureus into the supernatant. OGP, the solvent of sphingosine, added at the same concentrations as in the samples with sphingosine, exerted no effect. Either Triton or nisin was added as a positive control for membrane permeabilization and ATP release into the medium. Displayed are the means ± S.D. of four independent experiments each in A , C , and D . ***, p
    Figure Legend Snippet: Sphingosine induces a rapid increase in the permeability of P. aeruginosa . A and B, P. aeruginosa ( P.a. ) strains 762 and ATCC 27853 or S. aureus ( S.a. ) strain DH was incubated with 100 n m TO-PRO-3 iodide. Bacteria were then treated for 5 min with 1 or 10 μ m sphingosine ( SPH ) or the corresponding concentration of octylglucopyranoside ( OGP ), the solvent of sphingosine. The bacteria were analyzed by flow cytometry. A shows the quantitative analysis of the means of the fluorescence intensity obtained in the flow cytometry studies (given in arbitrary units; a.u. ), and B shows representative flow cytometry stainings from four independent experiments. Either Triton X-100 or nisin was added as a positive control for membrane permeabilization. C and D , intracellular ATP in P. aeruginosa strains 762 and ATCC 27853 or S. aureus strain DH ( C ) and the release of ATP ( D ) from the bacteria were measured with the BacTiter-Glo reagent. SPH results in a very rapid release of ATP from P. aeruginosa or S. aureus into the supernatant. OGP, the solvent of sphingosine, added at the same concentrations as in the samples with sphingosine, exerted no effect. Either Triton or nisin was added as a positive control for membrane permeabilization and ATP release into the medium. Displayed are the means ± S.D. of four independent experiments each in A , C , and D . ***, p

    Techniques Used: Permeability, Incubation, Concentration Assay, Flow Cytometry, Fluorescence, Positive Control

    NH 2 group and protonation of this group are required for the bactericidal effects of sphingosine on P. aeruginosa . A , each of the 10,000 cfu of P. aeruginosa ( P.a. ) strains 762 and ATCC 27853 was incubated with 1, 5, or 10 μ m stearylamine or SPH or 0.0005, 0.0025, or 0.005% of the solvent OGP in PBS adjusted to pH 7.0. After 60 min, the bacteria were washed; aliquots were plated on trypticase soy broth plates, and cfu were counted after growth overnight. Shown are the means ± S.D. of four independent experiments each. ***, p
    Figure Legend Snippet: NH 2 group and protonation of this group are required for the bactericidal effects of sphingosine on P. aeruginosa . A , each of the 10,000 cfu of P. aeruginosa ( P.a. ) strains 762 and ATCC 27853 was incubated with 1, 5, or 10 μ m stearylamine or SPH or 0.0005, 0.0025, or 0.005% of the solvent OGP in PBS adjusted to pH 7.0. After 60 min, the bacteria were washed; aliquots were plated on trypticase soy broth plates, and cfu were counted after growth overnight. Shown are the means ± S.D. of four independent experiments each. ***, p

    Techniques Used: Incubation

    Sphingosine mediates killing of bacteria within minutes. Each of the 10,000 cfu of P. aeruginosa ( P.a. ) strains 762 and ATCC 27853 was incubated in PBS (pH 7.0) with 1 or 10 μ m SPH or the corresponding concentrations of the solvent OGP for 15 or 60 min. Samples were washed, and aliquots were plated on trypticase soy broth plates and were counted after growth overnight. Shown are the means ± S.D. of four independent experiments each. ***, p
    Figure Legend Snippet: Sphingosine mediates killing of bacteria within minutes. Each of the 10,000 cfu of P. aeruginosa ( P.a. ) strains 762 and ATCC 27853 was incubated in PBS (pH 7.0) with 1 or 10 μ m SPH or the corresponding concentrations of the solvent OGP for 15 or 60 min. Samples were washed, and aliquots were plated on trypticase soy broth plates and were counted after growth overnight. Shown are the means ± S.D. of four independent experiments each. ***, p

    Techniques Used: Incubation

    5) Product Images from "Characterization of an ADP-Ribosyltransferase Toxin (AexT) from Aeromonas salmonicida subsp. salmonicida"

    Article Title: Characterization of an ADP-Ribosyltransferase Toxin (AexT) from Aeromonas salmonicida subsp. salmonicida

    Journal: Journal of Bacteriology

    doi: 10.1128/JB.184.7.1851-1858.2002

    Expression of AexT by A. salmonicida subsp. salmonicida in low-Ca 2+ medium and serological cross-reactions with ExoS and ExoT. Bacterial cultures were grown in Ca 2+ -depleted TSB medium. Lane 1, A. salmonicida subsp. salmonicida wt strain JF2267; lane 2, A. salmonicida subsp. salmonicida aexT mutant JF2580; lane 3, P. aeruginosa strain ATCC 27853. Culture supernatants were concentrated 20-fold and analyzed on immunoblots with anti-AexT antibodies. Lane c, purified recombinant AexT-His as a control; lane st, molecular mass standard. The identity of the band at 30 kDa reacting with P. aeruginosa ATCC 27853 (lane 3) is not determined.
    Figure Legend Snippet: Expression of AexT by A. salmonicida subsp. salmonicida in low-Ca 2+ medium and serological cross-reactions with ExoS and ExoT. Bacterial cultures were grown in Ca 2+ -depleted TSB medium. Lane 1, A. salmonicida subsp. salmonicida wt strain JF2267; lane 2, A. salmonicida subsp. salmonicida aexT mutant JF2580; lane 3, P. aeruginosa strain ATCC 27853. Culture supernatants were concentrated 20-fold and analyzed on immunoblots with anti-AexT antibodies. Lane c, purified recombinant AexT-His as a control; lane st, molecular mass standard. The identity of the band at 30 kDa reacting with P. aeruginosa ATCC 27853 (lane 3) is not determined.

    Techniques Used: Expressing, Mutagenesis, Western Blot, Purification, Recombinant

    6) Product Images from "Acid Ceramidase Rescues Cystic Fibrosis Mice from Pulmonary Infections"

    Article Title: Acid Ceramidase Rescues Cystic Fibrosis Mice from Pulmonary Infections

    Journal: Infection and Immunity

    doi: 10.1128/IAI.00677-20

    Sphingosine mediates killing of P. aeruginosa . (A) Comparison of the sensitivities of P. aeruginosa strains 762, 1242, ATCC 27853, and the sphingosine-resistant P. aeruginosa ATCC 27853 strain (ATCC 27853resSPH [ATCC-res]). A total of 1,000 CFU were incubated in H/S for 60 min with increasing concentrations of sphingosine, aliquots were plated on LB agar, and CFU were counted after growth overnight. Shown are the means ± SD from 4 independent experiments. **, P
    Figure Legend Snippet: Sphingosine mediates killing of P. aeruginosa . (A) Comparison of the sensitivities of P. aeruginosa strains 762, 1242, ATCC 27853, and the sphingosine-resistant P. aeruginosa ATCC 27853 strain (ATCC 27853resSPH [ATCC-res]). A total of 1,000 CFU were incubated in H/S for 60 min with increasing concentrations of sphingosine, aliquots were plated on LB agar, and CFU were counted after growth overnight. Shown are the means ± SD from 4 independent experiments. **, P

    Techniques Used: Incubation

    Overexpression of acid ceramidase protects CF mice from pulmonary infection with P. aeruginosa . (A and B) Cystic fibrosis (CF), wild-type (WT), Asah1tg, or CF-Asah1tg mice were infected with P. aeruginosa strains 762 (A), ATCC 27853 (B), and 1242 (C) for 4 h. Pneumonia was determined by using a sickness score and measuring the number of bacteria (CFU) in the lung 4 h after infection. The overexpression of acid ceramidase protected CF mice from pulmonary infection with P. aeruginosa . Shown are the means ± SD from 6 mice each. ***, P
    Figure Legend Snippet: Overexpression of acid ceramidase protects CF mice from pulmonary infection with P. aeruginosa . (A and B) Cystic fibrosis (CF), wild-type (WT), Asah1tg, or CF-Asah1tg mice were infected with P. aeruginosa strains 762 (A), ATCC 27853 (B), and 1242 (C) for 4 h. Pneumonia was determined by using a sickness score and measuring the number of bacteria (CFU) in the lung 4 h after infection. The overexpression of acid ceramidase protected CF mice from pulmonary infection with P. aeruginosa . Shown are the means ± SD from 6 mice each. ***, P

    Techniques Used: Over Expression, Mouse Assay, Infection

    7) Product Images from "X-ray Irradiated Vaccine Confers protection against Pneumonia caused by Pseudomonas Aeruginosa"

    Article Title: X-ray Irradiated Vaccine Confers protection against Pneumonia caused by Pseudomonas Aeruginosa

    Journal: Scientific Reports

    doi: 10.1038/srep18823

    Evaluation of the vaccine induced protection against P. aeruginosa pneumonia in vivo . Immunized C57BL/6 mice received escalating doses of 10 8 , 5 × 10 8 , 10 9 , and 5 × 10 9 CFUs irradiated ATCC27853 cells intra-nasally every week. 1 × 10 7 spleen lymphocytes or 300 μl serum were isolated from the immunized mice and transferred to normal mice 12 h before and after challenge, while controls received normal saline. Kaplan–Meier curves were plotted for mice of the above groups which were challenged by 5 × 10 6 CFUs the parental strain ATCC 27853 ( A ), 5 × 10 6 CFUs homologous serotype PAO-1( B ) and 1 × 10 7 CFUs heterologous serotype PAO-6 ( C ), and monitored the seven days survival rates. Irradiated vaccine protected mice against intranasal challenge by virulent P. aeruginosa , serum transferred mice showed powerful anti-infectious effect against homologous strain (ATCC 27853 and PAO-1) while T lymphocytes adopter mice survived longer when challenged by the homologous and heterologous serotype strains (ATCC 27853, PAO-1, and PAO-6) than the controls. Results represent three independent experiments (n = 10, *p
    Figure Legend Snippet: Evaluation of the vaccine induced protection against P. aeruginosa pneumonia in vivo . Immunized C57BL/6 mice received escalating doses of 10 8 , 5 × 10 8 , 10 9 , and 5 × 10 9 CFUs irradiated ATCC27853 cells intra-nasally every week. 1 × 10 7 spleen lymphocytes or 300 μl serum were isolated from the immunized mice and transferred to normal mice 12 h before and after challenge, while controls received normal saline. Kaplan–Meier curves were plotted for mice of the above groups which were challenged by 5 × 10 6 CFUs the parental strain ATCC 27853 ( A ), 5 × 10 6 CFUs homologous serotype PAO-1( B ) and 1 × 10 7 CFUs heterologous serotype PAO-6 ( C ), and monitored the seven days survival rates. Irradiated vaccine protected mice against intranasal challenge by virulent P. aeruginosa , serum transferred mice showed powerful anti-infectious effect against homologous strain (ATCC 27853 and PAO-1) while T lymphocytes adopter mice survived longer when challenged by the homologous and heterologous serotype strains (ATCC 27853, PAO-1, and PAO-6) than the controls. Results represent three independent experiments (n = 10, *p

    Techniques Used: In Vivo, Mouse Assay, Irradiation, Isolation

    Irradiation inhibits P. aeruginosa reproductive viability but does not impair metabolic activity. Increased exposure time to X-ray irradiation or heat (65 °C) resulted in decreased reproductive viability ( A,B ) and metabolic activity ( C,D ) of ATCC 27853 cells. Bacterial viability was determined by counting CFUs on agar plates, and metabolic activity was measured by monitoring the OD using Alamar Blue colorimetric assay. X ray irradiation diminished ATCC 27853 reproductive viability but does not impair its metabolic activity. The arrow denotes the irradiation dose, which could reduce the reproductive viability to 0% while retained 63.33% ± 4.49% metabolic activity compared with the live cells ( C ). Results represent three independent experiments and are expressed as mean ± SD (n = 5).
    Figure Legend Snippet: Irradiation inhibits P. aeruginosa reproductive viability but does not impair metabolic activity. Increased exposure time to X-ray irradiation or heat (65 °C) resulted in decreased reproductive viability ( A,B ) and metabolic activity ( C,D ) of ATCC 27853 cells. Bacterial viability was determined by counting CFUs on agar plates, and metabolic activity was measured by monitoring the OD using Alamar Blue colorimetric assay. X ray irradiation diminished ATCC 27853 reproductive viability but does not impair its metabolic activity. The arrow denotes the irradiation dose, which could reduce the reproductive viability to 0% while retained 63.33% ± 4.49% metabolic activity compared with the live cells ( C ). Results represent three independent experiments and are expressed as mean ± SD (n = 5).

    Techniques Used: Irradiation, Activity Assay, Colorimetric Assay

    8) Product Images from "Virulence factors in multidrug (MDR) and Pan-drug resistant (XDR) Pseudomonas aeruginosa: a cross-sectional study of isolates recovered from ocular infections in a high-incidence setting in southern India"

    Article Title: Virulence factors in multidrug (MDR) and Pan-drug resistant (XDR) Pseudomonas aeruginosa: a cross-sectional study of isolates recovered from ocular infections in a high-incidence setting in southern India

    Journal: Journal of Ophthalmic Inflammation and Infection

    doi: 10.1186/s12348-021-00268-w

    Heat map representing virulence factors of ocular clinical isolates of P. aeruginosa . The Virulence factors for 46 clinical isolates were tested by different methods specified earlier. Heat map was constructed to compare the S-PA and MDR-PA isolates. S1-S23 denotes drug susceptible strains, R1-R23 denotes MDR-PA strains. Blue represents a lower level of expression and yellow represents a higher level of expression
    Figure Legend Snippet: Heat map representing virulence factors of ocular clinical isolates of P. aeruginosa . The Virulence factors for 46 clinical isolates were tested by different methods specified earlier. Heat map was constructed to compare the S-PA and MDR-PA isolates. S1-S23 denotes drug susceptible strains, R1-R23 denotes MDR-PA strains. Blue represents a lower level of expression and yellow represents a higher level of expression

    Techniques Used: Construct, Expressing

    Clinical isolates of P. aeruginosa were plated on MHA at indicated time points. Bacterial load and viability were enumerated by plate count method. Student’s test was used for the statistical analysis and data are represented as the mean colony forming units (CFU ± SD) from three sets of independent experiments. * p
    Figure Legend Snippet: Clinical isolates of P. aeruginosa were plated on MHA at indicated time points. Bacterial load and viability were enumerated by plate count method. Student’s test was used for the statistical analysis and data are represented as the mean colony forming units (CFU ± SD) from three sets of independent experiments. * p

    Techniques Used:

    9) Product Images from "Acid Ceramidase Rescues Cystic Fibrosis Mice from Pulmonary Infections"

    Article Title: Acid Ceramidase Rescues Cystic Fibrosis Mice from Pulmonary Infections

    Journal: Infection and Immunity

    doi: 10.1128/IAI.00677-20

    Sphingosine mediates killing of P. aeruginosa . (A) Comparison of the sensitivities of P. aeruginosa strains 762, 1242, ATCC 27853, and the sphingosine-resistant P. aeruginosa ATCC 27853 strain (ATCC 27853resSPH [ATCC-res]). A total of 1,000 CFU were incubated in H/S for 60 min with increasing concentrations of sphingosine, aliquots were plated on LB agar, and CFU were counted after growth overnight. Shown are the means ± SD from 4 independent experiments. **, P
    Figure Legend Snippet: Sphingosine mediates killing of P. aeruginosa . (A) Comparison of the sensitivities of P. aeruginosa strains 762, 1242, ATCC 27853, and the sphingosine-resistant P. aeruginosa ATCC 27853 strain (ATCC 27853resSPH [ATCC-res]). A total of 1,000 CFU were incubated in H/S for 60 min with increasing concentrations of sphingosine, aliquots were plated on LB agar, and CFU were counted after growth overnight. Shown are the means ± SD from 4 independent experiments. **, P

    Techniques Used: Incubation

    Overexpression of acid ceramidase protects CF mice from pulmonary infection with P. aeruginosa . (A and B) Cystic fibrosis (CF), wild-type (WT), Asah1tg, or CF-Asah1tg mice were infected with P. aeruginosa strains 762 (A), ATCC 27853 (B), and 1242 (C) for 4 h. Pneumonia was determined by using a sickness score and measuring the number of bacteria (CFU) in the lung 4 h after infection. The overexpression of acid ceramidase protected CF mice from pulmonary infection with P. aeruginosa . Shown are the means ± SD from 6 mice each. ***, P
    Figure Legend Snippet: Overexpression of acid ceramidase protects CF mice from pulmonary infection with P. aeruginosa . (A and B) Cystic fibrosis (CF), wild-type (WT), Asah1tg, or CF-Asah1tg mice were infected with P. aeruginosa strains 762 (A), ATCC 27853 (B), and 1242 (C) for 4 h. Pneumonia was determined by using a sickness score and measuring the number of bacteria (CFU) in the lung 4 h after infection. The overexpression of acid ceramidase protected CF mice from pulmonary infection with P. aeruginosa . Shown are the means ± SD from 6 mice each. ***, P

    Techniques Used: Over Expression, Mouse Assay, Infection

    10) Product Images from "In Vitro Activity of Ceftolozane-Tazobactam against Multidrug-Resistant Nonfermenting Gram-Negative Bacilli Isolated from Patients with Cystic Fibrosis"

    Article Title: In Vitro Activity of Ceftolozane-Tazobactam against Multidrug-Resistant Nonfermenting Gram-Negative Bacilli Isolated from Patients with Cystic Fibrosis

    Journal: Antimicrobial Agents and Chemotherapy

    doi: 10.1128/AAC.02688-16

    Time-kill curves for P. aeruginosa ATCC 27853 exposed to antimicrobial agents at 4× MIC (A) or 8× MIC (B). Antibiotics were added to the medium after 2 h of incubation. □, growth control; ▲, ceftolozane alone; △, ceftolozane-tazobactam; ●, ceftazidime; ■, piperacillin-tazobactam; ◆, meropenem.
    Figure Legend Snippet: Time-kill curves for P. aeruginosa ATCC 27853 exposed to antimicrobial agents at 4× MIC (A) or 8× MIC (B). Antibiotics were added to the medium after 2 h of incubation. □, growth control; ▲, ceftolozane alone; △, ceftolozane-tazobactam; ●, ceftazidime; ■, piperacillin-tazobactam; ◆, meropenem.

    Techniques Used: Incubation

    11) Product Images from "Acid Ceramidase Rescues Cystic Fibrosis Mice from Pulmonary Infections"

    Article Title: Acid Ceramidase Rescues Cystic Fibrosis Mice from Pulmonary Infections

    Journal: Infection and Immunity

    doi: 10.1128/IAI.00677-20

    Sphingosine mediates killing of P. aeruginosa . (A) Comparison of the sensitivities of P. aeruginosa strains 762, 1242, ATCC 27853, and the sphingosine-resistant P. aeruginosa ATCC 27853 strain (ATCC 27853resSPH [ATCC-res]). A total of 1,000 CFU were incubated in H/S for 60 min with increasing concentrations of sphingosine, aliquots were plated on LB agar, and CFU were counted after growth overnight. Shown are the means ± SD from 4 independent experiments. **, P
    Figure Legend Snippet: Sphingosine mediates killing of P. aeruginosa . (A) Comparison of the sensitivities of P. aeruginosa strains 762, 1242, ATCC 27853, and the sphingosine-resistant P. aeruginosa ATCC 27853 strain (ATCC 27853resSPH [ATCC-res]). A total of 1,000 CFU were incubated in H/S for 60 min with increasing concentrations of sphingosine, aliquots were plated on LB agar, and CFU were counted after growth overnight. Shown are the means ± SD from 4 independent experiments. **, P

    Techniques Used: Incubation

    Overexpression of acid ceramidase protects CF mice from pulmonary infection with P. aeruginosa . (A and B) Cystic fibrosis (CF), wild-type (WT), Asah1tg, or CF-Asah1tg mice were infected with P. aeruginosa strains 762 (A), ATCC 27853 (B), and 1242 (C) for 4 h. Pneumonia was determined by using a sickness score and measuring the number of bacteria (CFU) in the lung 4 h after infection. The overexpression of acid ceramidase protected CF mice from pulmonary infection with P. aeruginosa . Shown are the means ± SD from 6 mice each. ***, P
    Figure Legend Snippet: Overexpression of acid ceramidase protects CF mice from pulmonary infection with P. aeruginosa . (A and B) Cystic fibrosis (CF), wild-type (WT), Asah1tg, or CF-Asah1tg mice were infected with P. aeruginosa strains 762 (A), ATCC 27853 (B), and 1242 (C) for 4 h. Pneumonia was determined by using a sickness score and measuring the number of bacteria (CFU) in the lung 4 h after infection. The overexpression of acid ceramidase protected CF mice from pulmonary infection with P. aeruginosa . Shown are the means ± SD from 6 mice each. ***, P

    Techniques Used: Over Expression, Mouse Assay, Infection

    12) Product Images from "Activity of AMP2041 against human and animal multidrug resistant Pseudomonas aeruginosa clinical isolates"

    Article Title: Activity of AMP2041 against human and animal multidrug resistant Pseudomonas aeruginosa clinical isolates

    Journal: Annals of Clinical Microbiology and Antimicrobials

    doi: 10.1186/s12941-017-0193-1

    SEM analysis performed on P. aeruginosa ATCC 27853. a Untreated; b treated with AMP2041; c holes size measurement
    Figure Legend Snippet: SEM analysis performed on P. aeruginosa ATCC 27853. a Untreated; b treated with AMP2041; c holes size measurement

    Techniques Used:

    13) Product Images from "In Vitro and In Vivo Assessment of the Efficacy of Bromoageliferin, an Alkaloid Isolated from the Sponge Agelas dilatata, against Pseudomonas aeruginosa"

    Article Title: In Vitro and In Vivo Assessment of the Efficacy of Bromoageliferin, an Alkaloid Isolated from the Sponge Agelas dilatata, against Pseudomonas aeruginosa

    Journal: Marine Drugs

    doi: 10.3390/md18060326

    Quantification of biofilm formation after 24 h by P. aeruginosa strains PAO1 and ATCC 27853 in the presence of different concentrations of bromoageliferin ( 2 ).
    Figure Legend Snippet: Quantification of biofilm formation after 24 h by P. aeruginosa strains PAO1 and ATCC 27853 in the presence of different concentrations of bromoageliferin ( 2 ).

    Techniques Used:

    Survival of G. mellonella larvae ( n = 15 per group) following infection with P. aeruginosa strain ATCC 27853 untreated (PBS) and treated with bromoageliferin ( 2 ) (2 mg/kg).
    Figure Legend Snippet: Survival of G. mellonella larvae ( n = 15 per group) following infection with P. aeruginosa strain ATCC 27853 untreated (PBS) and treated with bromoageliferin ( 2 ) (2 mg/kg).

    Techniques Used: Infection

    Influence of the presence of bromine atoms in A and B pyrrol rings in the antibacterial activity of compounds 1 – 3 against P. aeruginosa ATCC 27853 strain.
    Figure Legend Snippet: Influence of the presence of bromine atoms in A and B pyrrol rings in the antibacterial activity of compounds 1 – 3 against P. aeruginosa ATCC 27853 strain.

    Techniques Used: Activity Assay

    14) Product Images from "Evaluation of Pharmacodynamic Interactions Between Telavancin and Aztreonam or Piperacillin/Tazobactam Against Pseudomonas aeruginosa, Escherichia coli and Methicillin-Resistant Staphylococcus aureus"

    Article Title: Evaluation of Pharmacodynamic Interactions Between Telavancin and Aztreonam or Piperacillin/Tazobactam Against Pseudomonas aeruginosa, Escherichia coli and Methicillin-Resistant Staphylococcus aureus

    Journal: Infectious Diseases and Therapy

    doi: 10.1007/s40121-016-0121-2

    In vitro activity of telavancin alone and in combination of aztreonam or piperacillin/tazobactam in a P. aeruginosa ATCC 27853. Black circle growth control, white circle telavancin, white square aztreonam, white inverted triangle piperacillin/tazobactam, black triangle telavancin + aztreonam, black inverted triangle telavancin + piperacillin/tazobactam
    Figure Legend Snippet: In vitro activity of telavancin alone and in combination of aztreonam or piperacillin/tazobactam in a P. aeruginosa ATCC 27853. Black circle growth control, white circle telavancin, white square aztreonam, white inverted triangle piperacillin/tazobactam, black triangle telavancin + aztreonam, black inverted triangle telavancin + piperacillin/tazobactam

    Techniques Used: In Vitro, Activity Assay

    15) Product Images from "Overexpression of BIT33_RS14560 Enhances the Biofilm Formation and Virulence of Acinetobacter baumannii"

    Article Title: Overexpression of BIT33_RS14560 Enhances the Biofilm Formation and Virulence of Acinetobacter baumannii

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2022.867770

    Kaplan–Meier curve analysis of the survival of G. mellonella after infection. PBS is the negative control group, ATCC 19606 is the wild-type strain, 19,606-p-RS14560 is the overexpression strain, 19,606-p is the empty plasmid control group, and A. baumannii 5075 is the positive control group. PBS, phosphate buffered saline. p
    Figure Legend Snippet: Kaplan–Meier curve analysis of the survival of G. mellonella after infection. PBS is the negative control group, ATCC 19606 is the wild-type strain, 19,606-p-RS14560 is the overexpression strain, 19,606-p is the empty plasmid control group, and A. baumannii 5075 is the positive control group. PBS, phosphate buffered saline. p

    Techniques Used: Infection, Negative Control, Over Expression, Plasmid Preparation, Positive Control

    The volcano plot of differentially expressed genes identified by RNA-Seq. The x -axis represents the log 2 (FC), and the y -axis represents –log 10 ( p-adjust ) calculated by the Student’s t -test. The red dots represent the upregulated genes with statistical significance ( p-adjust
    Figure Legend Snippet: The volcano plot of differentially expressed genes identified by RNA-Seq. The x -axis represents the log 2 (FC), and the y -axis represents –log 10 ( p-adjust ) calculated by the Student’s t -test. The red dots represent the upregulated genes with statistical significance ( p-adjust

    Techniques Used: RNA Sequencing Assay

    Relative expression of BIT33_RS14560 by qRT-PCR. The x -axis represents the A. baumannii strains, and the y -axis represents the relative mRNA levels of BIT33_RS14560 (2 –△CT ). p
    Figure Legend Snippet: Relative expression of BIT33_RS14560 by qRT-PCR. The x -axis represents the A. baumannii strains, and the y -axis represents the relative mRNA levels of BIT33_RS14560 (2 –△CT ). p

    Techniques Used: Expressing, Quantitative RT-PCR

    The dynamic changes of biofilm biomass produced by the nine A. baumannii strains at different time points. ATCC 19606, XAb53, and XAb50 are wild-type strains. 19,606-p-RS14560, XAb53-p-telR-RS14560, and XAb50-p-telR-RS14560 are BIT33_RS14560 -overexpressed strains. 19,606-p, XAb53-p-telR, and XAb50-p-telR are empty plasmid control strains. The overexpression of BIT33_RS14560 exhibits enhancing eff ects, but with various time patterns, on the biofilm formation of strains in the experimental groups. Values are means ± SD of over three independent experiments. (A) Wild-type and recombinant strains of ATCC 19606; (B) Wild-type and recombinant strains of XAb53; and (C) Wild-type and recombinant strains of XAb50.
    Figure Legend Snippet: The dynamic changes of biofilm biomass produced by the nine A. baumannii strains at different time points. ATCC 19606, XAb53, and XAb50 are wild-type strains. 19,606-p-RS14560, XAb53-p-telR-RS14560, and XAb50-p-telR-RS14560 are BIT33_RS14560 -overexpressed strains. 19,606-p, XAb53-p-telR, and XAb50-p-telR are empty plasmid control strains. The overexpression of BIT33_RS14560 exhibits enhancing eff ects, but with various time patterns, on the biofilm formation of strains in the experimental groups. Values are means ± SD of over three independent experiments. (A) Wild-type and recombinant strains of ATCC 19606; (B) Wild-type and recombinant strains of XAb53; and (C) Wild-type and recombinant strains of XAb50.

    Techniques Used: Produced, Plasmid Preparation, Over Expression, Recombinant

    Around 1% agarose gel electrophoresis of PCR products indicates a successful construction of p-telR-RS14560 vector. The AmpR fragment in the p-RS14560 plasmid is replaced by telR cassette amplified from the pMo130telR vector. Lane 1: PCR products of telR cassette; lane 2: PCR products of p-RS14560 plasmid as negative control, lane 3: PCR products of positive clone, and lane 4: DNA marker 5,000 bp.
    Figure Legend Snippet: Around 1% agarose gel electrophoresis of PCR products indicates a successful construction of p-telR-RS14560 vector. The AmpR fragment in the p-RS14560 plasmid is replaced by telR cassette amplified from the pMo130telR vector. Lane 1: PCR products of telR cassette; lane 2: PCR products of p-RS14560 plasmid as negative control, lane 3: PCR products of positive clone, and lane 4: DNA marker 5,000 bp.

    Techniques Used: Agarose Gel Electrophoresis, Polymerase Chain Reaction, Plasmid Preparation, Amplification, Negative Control, Marker

    Heatmap of differentially expressed genes. 19,606-p-RS14,560_1 and 19,606-p-RS14,560_2 represent the treated groups while 19,606-p_1 and 19,606-p_2 represent the control groups. FC, fold change.
    Figure Legend Snippet: Heatmap of differentially expressed genes. 19,606-p-RS14,560_1 and 19,606-p-RS14,560_2 represent the treated groups while 19,606-p_1 and 19,606-p_2 represent the control groups. FC, fold change.

    Techniques Used:

    16) Product Images from "Negatively Charged Lipids as a Potential Target for New Amphiphilic Aminoglycoside Antibiotics"

    Article Title: Negatively Charged Lipids as a Potential Target for New Amphiphilic Aminoglycoside Antibiotics

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M115.665364

    A , phase-contrast images of P. aeruginosa ATCC 27853 non-treated ( left ) and treated ( right ) with 3′,6-dinonyl neamine at its minimum inhibitory concentration for 7 h at 37 °C. Black scale bar , 4 μm. B , outer (▴) and inner
    Figure Legend Snippet: A , phase-contrast images of P. aeruginosa ATCC 27853 non-treated ( left ) and treated ( right ) with 3′,6-dinonyl neamine at its minimum inhibitory concentration for 7 h at 37 °C. Black scale bar , 4 μm. B , outer (▴) and inner

    Techniques Used: Concentration Assay

    17) Product Images from "A quorum sensing-regulated type VI secretion system containing multiple nonredundant VgrG proteins is required for interbacterial competition in Chromobacterium violaceum"

    Article Title: A quorum sensing-regulated type VI secretion system containing multiple nonredundant VgrG proteins is required for interbacterial competition in Chromobacterium violaceum

    Journal: bioRxiv

    doi: 10.1101/2022.04.29.490089

    C. violaceum depends on CviR but not on CviI for the assembly of a functional T6SS. (A) Effects of cell density, CviR and CviI on Hcp production and secretion. Western blot of Hcp in cellular and secreted fractions of WT, Δ cviR , and Δ cviI strains at different points of the growth curve: ML, mid-log phase; LL, late log phase; ES, early stationary; LL, late stationary. (B) Fluorescence microscopy showing T6SS sheath assembly through the formation of VipA_sfGFP foci. Scale = 3 µm. The graph shows the average number of foci per cell. (C) Recovery of P. aeruginosa ATCC 27853 after 4 hours in coculture with the indicated C. violaceum strains. Bars indicate the mean and SD. Statistical analyses were performed using the unpaired Student’s t test, and asterisks indicate variations in relation to the WT. ***p
    Figure Legend Snippet: C. violaceum depends on CviR but not on CviI for the assembly of a functional T6SS. (A) Effects of cell density, CviR and CviI on Hcp production and secretion. Western blot of Hcp in cellular and secreted fractions of WT, Δ cviR , and Δ cviI strains at different points of the growth curve: ML, mid-log phase; LL, late log phase; ES, early stationary; LL, late stationary. (B) Fluorescence microscopy showing T6SS sheath assembly through the formation of VipA_sfGFP foci. Scale = 3 µm. The graph shows the average number of foci per cell. (C) Recovery of P. aeruginosa ATCC 27853 after 4 hours in coculture with the indicated C. violaceum strains. Bars indicate the mean and SD. Statistical analyses were performed using the unpaired Student’s t test, and asterisks indicate variations in relation to the WT. ***p

    Techniques Used: Functional Assay, Western Blot, Fluorescence, Microscopy

    VgrG3 is the most important VgrG for C. violaceum antibacterial activity. A quantitative antibacterial assay was performed by counting the CFU of P. aeruginosa ATCC 27853 without (LB) or after coculture with C. violaceum . Competitions were performed using (A) the individual vgrG mutants, (B) the complemented Δ vgrG 3( vgrG3 ) strain, (C) the sequential vgrG mutants, and (D) the sextuple Δ vgrG1-6 mutant harboring each single vgrG gene. Each point represents biological replicates of distinct assays. Bars show the mean and SD. Statistical analyses were performed using the unpaired Student’s t test, and asterisks indicate variations in relation to the WT. *p
    Figure Legend Snippet: VgrG3 is the most important VgrG for C. violaceum antibacterial activity. A quantitative antibacterial assay was performed by counting the CFU of P. aeruginosa ATCC 27853 without (LB) or after coculture with C. violaceum . Competitions were performed using (A) the individual vgrG mutants, (B) the complemented Δ vgrG 3( vgrG3 ) strain, (C) the sequential vgrG mutants, and (D) the sextuple Δ vgrG1-6 mutant harboring each single vgrG gene. Each point represents biological replicates of distinct assays. Bars show the mean and SD. Statistical analyses were performed using the unpaired Student’s t test, and asterisks indicate variations in relation to the WT. *p

    Techniques Used: Activity Assay, Mutagenesis

    The T6SS contributes to interbacterial competition but not to virulence in C. violaceum . (A) C. violaceum outcompetes many Gram-negative bacteria via the T6SS. Qualitative growth competition assay of C. violaceum WT or T6SS mutants against the indicated bacteria (details in Table S3). Predominantly purple spots (due to violacein production by C. violaceum ) indicate the cocultures in which C. violaceum was able to outcompete the target bacteria. The C. violaceum T6SS showed a major role in competition against E. coli (Ec), P. aeruginosa ATCC 27853 (Pa) and S. maltophilia (Sma). (B) C. violaceum employs its T6SS to kill P. aeruginosa . A quantitative antibacterial assay was performed by counting the CFU of P. aeruginosa ATCC 27853 without (LB) or after coculture with C. violaceum WT, T6SS mutants (Δ hcp and Δ vipA ) or complemented strains. Data are from at least three independent biological assays. Bars show the mean and SD. Statistical analyses were performed using the unpaired Student’s t test, and asterisks indicate variations in relation to the WT. *p
    Figure Legend Snippet: The T6SS contributes to interbacterial competition but not to virulence in C. violaceum . (A) C. violaceum outcompetes many Gram-negative bacteria via the T6SS. Qualitative growth competition assay of C. violaceum WT or T6SS mutants against the indicated bacteria (details in Table S3). Predominantly purple spots (due to violacein production by C. violaceum ) indicate the cocultures in which C. violaceum was able to outcompete the target bacteria. The C. violaceum T6SS showed a major role in competition against E. coli (Ec), P. aeruginosa ATCC 27853 (Pa) and S. maltophilia (Sma). (B) C. violaceum employs its T6SS to kill P. aeruginosa . A quantitative antibacterial assay was performed by counting the CFU of P. aeruginosa ATCC 27853 without (LB) or after coculture with C. violaceum WT, T6SS mutants (Δ hcp and Δ vipA ) or complemented strains. Data are from at least three independent biological assays. Bars show the mean and SD. Statistical analyses were performed using the unpaired Student’s t test, and asterisks indicate variations in relation to the WT. *p

    Techniques Used: Competitive Binding Assay

    VgrG3 interacts directly with other VgrGs  in vivo . (A) Confirmation of VgrG3-HA expression. Western blot with a monoclonal anti-HA antibody revealed VgrG3-HA expression (predicted molecular weight of 110 kDa) in the Δ vgrG 3 strain harboring a vector expressing the construct  vgrG 3-HA. (B) VgrG3-HA functionally complements the Δ vgrG 3 mutant strain. Interbacterial competition of the  C. violaceum  indicated strains against  P. aeruginosa  ATCC 27853. Competitive fitness was rescued in the Δ vgrG 3( vgrG 3-HA) strain. Bars indicate the mean and SD. Statistical analyses were performed using the unpaired Student’s t test, and asterisks indicate variations in relation to the WT. *p
    Figure Legend Snippet: VgrG3 interacts directly with other VgrGs in vivo . (A) Confirmation of VgrG3-HA expression. Western blot with a monoclonal anti-HA antibody revealed VgrG3-HA expression (predicted molecular weight of 110 kDa) in the Δ vgrG 3 strain harboring a vector expressing the construct vgrG 3-HA. (B) VgrG3-HA functionally complements the Δ vgrG 3 mutant strain. Interbacterial competition of the C. violaceum indicated strains against P. aeruginosa ATCC 27853. Competitive fitness was rescued in the Δ vgrG 3( vgrG 3-HA) strain. Bars indicate the mean and SD. Statistical analyses were performed using the unpaired Student’s t test, and asterisks indicate variations in relation to the WT. *p

    Techniques Used: In Vivo, Expressing, Western Blot, Molecular Weight, Plasmid Preparation, Construct, Mutagenesis

    18) Product Images from "Acid Ceramidase Rescues Cystic Fibrosis Mice from Pulmonary Infections"

    Article Title: Acid Ceramidase Rescues Cystic Fibrosis Mice from Pulmonary Infections

    Journal: Infection and Immunity

    doi: 10.1128/IAI.00677-20

    Sphingosine mediates killing of P. aeruginosa . (A) Comparison of the sensitivities of P. aeruginosa strains 762, 1242, ATCC 27853, and the sphingosine-resistant P. aeruginosa ATCC 27853 strain (ATCC 27853resSPH [ATCC-res]). A total of 1,000 CFU were incubated in H/S for 60 min with increasing concentrations of sphingosine, aliquots were plated on LB agar, and CFU were counted after growth overnight. Shown are the means ± SD from 4 independent experiments. **, P
    Figure Legend Snippet: Sphingosine mediates killing of P. aeruginosa . (A) Comparison of the sensitivities of P. aeruginosa strains 762, 1242, ATCC 27853, and the sphingosine-resistant P. aeruginosa ATCC 27853 strain (ATCC 27853resSPH [ATCC-res]). A total of 1,000 CFU were incubated in H/S for 60 min with increasing concentrations of sphingosine, aliquots were plated on LB agar, and CFU were counted after growth overnight. Shown are the means ± SD from 4 independent experiments. **, P

    Techniques Used: Incubation

    Overexpression of acid ceramidase protects CF mice from pulmonary infection with P. aeruginosa . (A and B) Cystic fibrosis (CF), wild-type (WT), Asah1tg, or CF-Asah1tg mice were infected with P. aeruginosa strains 762 (A), ATCC 27853 (B), and 1242 (C) for 4 h. Pneumonia was determined by using a sickness score and measuring the number of bacteria (CFU) in the lung 4 h after infection. The overexpression of acid ceramidase protected CF mice from pulmonary infection with P. aeruginosa . Shown are the means ± SD from 6 mice each. ***, P
    Figure Legend Snippet: Overexpression of acid ceramidase protects CF mice from pulmonary infection with P. aeruginosa . (A and B) Cystic fibrosis (CF), wild-type (WT), Asah1tg, or CF-Asah1tg mice were infected with P. aeruginosa strains 762 (A), ATCC 27853 (B), and 1242 (C) for 4 h. Pneumonia was determined by using a sickness score and measuring the number of bacteria (CFU) in the lung 4 h after infection. The overexpression of acid ceramidase protected CF mice from pulmonary infection with P. aeruginosa . Shown are the means ± SD from 6 mice each. ***, P

    Techniques Used: Over Expression, Mouse Assay, Infection

    19) Product Images from "Multicenter Evaluation of Colistin Broth Disk Elution and Colistin Agar Test: a Report from the Clinical and Laboratory Standards Institute"

    Article Title: Multicenter Evaluation of Colistin Broth Disk Elution and Colistin Agar Test: a Report from the Clinical and Laboratory Standards Institute

    Journal: Journal of Clinical Microbiology

    doi: 10.1128/JCM.01269-19

    Colistin MIC distribution for quality control indicator strains P. aeruginosa ATCC 27853 (left panel) and E. coli CDC FDA AR no. 0349 (right panel) by the CBDE, CAT-1, CAT-10, and rBMD methods. The lowest concentrations tested were 0.4 μg/ml for CBDE, 0.5 μg/ml for CAT-1 and CAT-10, and 0.25 μg/ml for BMD.
    Figure Legend Snippet: Colistin MIC distribution for quality control indicator strains P. aeruginosa ATCC 27853 (left panel) and E. coli CDC FDA AR no. 0349 (right panel) by the CBDE, CAT-1, CAT-10, and rBMD methods. The lowest concentrations tested were 0.4 μg/ml for CBDE, 0.5 μg/ml for CAT-1 and CAT-10, and 0.25 μg/ml for BMD.

    Techniques Used:

    20) Product Images from "The Semi-Synthetic Peptide Lin-SB056-1 in Combination with EDTA Exerts Strong Antimicrobial and Antibiofilm Activity against Pseudomonas aeruginosa in Conditions Mimicking Cystic Fibrosis Sputum"

    Article Title: The Semi-Synthetic Peptide Lin-SB056-1 in Combination with EDTA Exerts Strong Antimicrobial and Antibiofilm Activity against Pseudomonas aeruginosa in Conditions Mimicking Cystic Fibrosis Sputum

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms18091994

    Visualization of a BLS under light microscopy at: ( a ) 400×; and ( b ) 1000× magnification, after 72 h of incubation; ( c ) Kinetics of lin-SB056-1 activity, used alone and in combination with EDTA, on the formation of BLSs of P. aeruginosa ATCC 27853 in ASM 80%. Data are reported as mean ± standard error of the mean of three independent experiments. * p
    Figure Legend Snippet: Visualization of a BLS under light microscopy at: ( a ) 400×; and ( b ) 1000× magnification, after 72 h of incubation; ( c ) Kinetics of lin-SB056-1 activity, used alone and in combination with EDTA, on the formation of BLSs of P. aeruginosa ATCC 27853 in ASM 80%. Data are reported as mean ± standard error of the mean of three independent experiments. * p

    Techniques Used: Light Microscopy, Incubation, Activity Assay

    Time kill curves of lin-SB056-1 at its bactericidal concentrations against: ( a ) P. aeruginosa ATCC 27853; and ( b ) P. aeruginosa PaM01 in 10 mM Sodium Phosphate Buffer (SPB) at pH 7.4 supplemented with 1% Tryptone Soya Broth (TSB). The concentrations of lin-SB056-1 (reported in parenthesis) are expressed in μg/mL. Control (CTRL) represents bacteria incubated in the absence of the peptide. Bactericidal activity was defined as a reduction in the numbers of viable bacteria of ≥3-log colony forming units (CFU)/mL at any incubation time tested. The dotted lines represent three-log reduction in CFU number, corresponding to the definition of bactericidal activity. Data are reported as mean ± standard error of the mean of three independent experiments.
    Figure Legend Snippet: Time kill curves of lin-SB056-1 at its bactericidal concentrations against: ( a ) P. aeruginosa ATCC 27853; and ( b ) P. aeruginosa PaM01 in 10 mM Sodium Phosphate Buffer (SPB) at pH 7.4 supplemented with 1% Tryptone Soya Broth (TSB). The concentrations of lin-SB056-1 (reported in parenthesis) are expressed in μg/mL. Control (CTRL) represents bacteria incubated in the absence of the peptide. Bactericidal activity was defined as a reduction in the numbers of viable bacteria of ≥3-log colony forming units (CFU)/mL at any incubation time tested. The dotted lines represent three-log reduction in CFU number, corresponding to the definition of bactericidal activity. Data are reported as mean ± standard error of the mean of three independent experiments.

    Techniques Used: Incubation, Activity Assay

    Activity of lin-SB056-1, used alone and in combination with EDTA, against preformed biofilms of P. aeruginosa : ( a ) ATCC 27853; and ( b ) PaM01 strain. The antibiofilm activity of the peptide, EDTA and peptide-EDTA combination was evaluated by crystal violet staining after 24 h of incubation. The concentrations of lin-SB056-1 and EDTA (reported in parenthesis) are expressed in μg/mL and mM, respectively. Control (CTRL) represents bacteria incubated in the absence of antimicrobial agents; comb: peptide/EDTA combination. Data are reported as mean ± standard error of the mean of three independent experiments. ** p
    Figure Legend Snippet: Activity of lin-SB056-1, used alone and in combination with EDTA, against preformed biofilms of P. aeruginosa : ( a ) ATCC 27853; and ( b ) PaM01 strain. The antibiofilm activity of the peptide, EDTA and peptide-EDTA combination was evaluated by crystal violet staining after 24 h of incubation. The concentrations of lin-SB056-1 and EDTA (reported in parenthesis) are expressed in μg/mL and mM, respectively. Control (CTRL) represents bacteria incubated in the absence of antimicrobial agents; comb: peptide/EDTA combination. Data are reported as mean ± standard error of the mean of three independent experiments. ** p

    Techniques Used: Activity Assay, Staining, Incubation

    Bactericidal activity of lin-SB056-1 used alone and in combination with EDTA against: ( a ) P. aeruginosa ATCC 27853; and ( b ) P. aeruginosa PaM01 strain in ASM 80% after 1.5 h of incubation. The concentrations of lin-SB056-1 and EDTA (reported in parenthesis) are expressed in μg/mL and mM, respectively. Control (CTRL) represents bacteria incubated in the absence of antimicrobial agents; comb: peptide/EDTA combination. Data are reported as mean ± standard error of the mean of three independent experiments. *** p
    Figure Legend Snippet: Bactericidal activity of lin-SB056-1 used alone and in combination with EDTA against: ( a ) P. aeruginosa ATCC 27853; and ( b ) P. aeruginosa PaM01 strain in ASM 80% after 1.5 h of incubation. The concentrations of lin-SB056-1 and EDTA (reported in parenthesis) are expressed in μg/mL and mM, respectively. Control (CTRL) represents bacteria incubated in the absence of antimicrobial agents; comb: peptide/EDTA combination. Data are reported as mean ± standard error of the mean of three independent experiments. *** p

    Techniques Used: Activity Assay, Incubation

    21) Product Images from "Oxidative responses and defense mechanism of hyperpigmented P. aeruginosa as characterized by proteomics and metabolomics"

    Article Title: Oxidative responses and defense mechanism of hyperpigmented P. aeruginosa as characterized by proteomics and metabolomics

    Journal: EXCLI Journal

    doi: 10.17179/excli2018-1238

    Absorption spectrum of dark-brown containing supernatant of Pseudomonas aeruginosa strain HP (straight line) and non dark-brown containing supernatant of ATCC 27853 (dot line). This measurement displayed a slightly increase in absorption from 700 nm to 430 nm and exponentially increases from 400 nm to 350 nm. The major absorption peak at 357 nm and minor peak (shoulder) at 400 nm were observed in P. aeruginosa HP supernatant, whereas the highest peak of P. aeruginosa ATCC 27853 supernatant was 380 nm with two shoulders at 320 and 400 nm (A). Different adsorption spectra was clearly detected when pigments were extracted and compared to pyocyanin (B).
    Figure Legend Snippet: Absorption spectrum of dark-brown containing supernatant of Pseudomonas aeruginosa strain HP (straight line) and non dark-brown containing supernatant of ATCC 27853 (dot line). This measurement displayed a slightly increase in absorption from 700 nm to 430 nm and exponentially increases from 400 nm to 350 nm. The major absorption peak at 357 nm and minor peak (shoulder) at 400 nm were observed in P. aeruginosa HP supernatant, whereas the highest peak of P. aeruginosa ATCC 27853 supernatant was 380 nm with two shoulders at 320 and 400 nm (A). Different adsorption spectra was clearly detected when pigments were extracted and compared to pyocyanin (B).

    Techniques Used: Adsorption

    2D-PAGE image or the master maps representing protein profiles of P. aeruginosa ATCC 27853 (panels A and C), high pigment produced strain P. aeruginosa HP (panels B and D) in the absence of paraquat (panels A and B) and present of 0.8 mM paraquat (panels C and D) which was separated under pH ranges of 3-10. Numbers of protein spot denoted as identified protein are represented in Table 1.
    Figure Legend Snippet: 2D-PAGE image or the master maps representing protein profiles of P. aeruginosa ATCC 27853 (panels A and C), high pigment produced strain P. aeruginosa HP (panels B and D) in the absence of paraquat (panels A and B) and present of 0.8 mM paraquat (panels C and D) which was separated under pH ranges of 3-10. Numbers of protein spot denoted as identified protein are represented in Table 1.

    Techniques Used: Polyacrylamide Gel Electrophoresis, Produced

    Free radical scavenging activity of extracted dark-brown pigment was determined using NBT assay. The dark-brown pigment extracted from P. aeruginosa shows the anti-oxidant activity effect which exerts its IC 50 at 0.26 mg/ml (B) compared to pigment extracted from ATCC 27853 reference strain which exerts its IC 50 at 0.67 mg/ml (A).
    Figure Legend Snippet: Free radical scavenging activity of extracted dark-brown pigment was determined using NBT assay. The dark-brown pigment extracted from P. aeruginosa shows the anti-oxidant activity effect which exerts its IC 50 at 0.26 mg/ml (B) compared to pigment extracted from ATCC 27853 reference strain which exerts its IC 50 at 0.67 mg/ml (A).

    Techniques Used: Activity Assay

    The comparison of pigment production in LB medium without any supplement from 24 h aerobic cultures of P. aeruginosa ATCC 27853 and P. aeruginosa high pigment clinically isolated strain HP. Brown zone was only observed around the colony of P. aeruginosa HP. Biochemical tests indicated varieties of pigment production based on the substrates utilized. Dark-brown pigment was observed on King P, King F, cetrimide and urea cultured medium but was not observed on glucose containing medium.
    Figure Legend Snippet: The comparison of pigment production in LB medium without any supplement from 24 h aerobic cultures of P. aeruginosa ATCC 27853 and P. aeruginosa high pigment clinically isolated strain HP. Brown zone was only observed around the colony of P. aeruginosa HP. Biochemical tests indicated varieties of pigment production based on the substrates utilized. Dark-brown pigment was observed on King P, King F, cetrimide and urea cultured medium but was not observed on glucose containing medium.

    Techniques Used: Isolation, Cell Culture

    22) Product Images from "Pseudomonas aeruginosa Pyocyanin Induces Neutrophil Death via Mitochondrial Reactive Oxygen Species and Mitochondrial Acid Sphingomyelinase"

    Article Title: Pseudomonas aeruginosa Pyocyanin Induces Neutrophil Death via Mitochondrial Reactive Oxygen Species and Mitochondrial Acid Sphingomyelinase

    Journal: Antioxidants & Redox Signaling

    doi: 10.1089/ars.2014.5979

    Acid sphingomyelinase expression is required for cell death and the release of cytochrome c from mitochondria upon infection with Pseudomonas aeruginosa. (A) HL-60 neutrophils or freshly isolated wild-type and Asm-deficient peritoneal neutrophils were infected for 8 h with P. aeruginosa strain ATCC 27853. Apoptosis was determined by staining with FITC-annexin V. Displayed are the means±SD, n =4; * p
    Figure Legend Snippet: Acid sphingomyelinase expression is required for cell death and the release of cytochrome c from mitochondria upon infection with Pseudomonas aeruginosa. (A) HL-60 neutrophils or freshly isolated wild-type and Asm-deficient peritoneal neutrophils were infected for 8 h with P. aeruginosa strain ATCC 27853. Apoptosis was determined by staining with FITC-annexin V. Displayed are the means±SD, n =4; * p

    Techniques Used: Expressing, Infection, Isolation, Staining

    23) Product Images from "Evaluation of Antimicrobial Activity of Triphala Constituents and Nanoformulation"

    Article Title: Evaluation of Antimicrobial Activity of Triphala Constituents and Nanoformulation

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    doi: 10.1155/2020/6976973

    Microdilution broth assay of TRIT 4–TRIT 8 against  P. aeruginosa  ATCC 27853.
    Figure Legend Snippet: Microdilution broth assay of TRIT 4–TRIT 8 against P. aeruginosa ATCC 27853.

    Techniques Used:

    Disc diffusion test of TRIT 1–TRIT 8 against  P. aeruginosa  ATCC 27853; TRIT 9 was the total Triphala hydroalcoholic extract.
    Figure Legend Snippet: Disc diffusion test of TRIT 1–TRIT 8 against P. aeruginosa ATCC 27853; TRIT 9 was the total Triphala hydroalcoholic extract.

    Techniques Used: Diffusion-based Assay

    24) Product Images from "Pseudomonas aeruginosa Pyocyanin Induces Neutrophil Death via Mitochondrial Reactive Oxygen Species and Mitochondrial Acid Sphingomyelinase"

    Article Title: Pseudomonas aeruginosa Pyocyanin Induces Neutrophil Death via Mitochondrial Reactive Oxygen Species and Mitochondrial Acid Sphingomyelinase

    Journal: Antioxidants & Redox Signaling

    doi: 10.1089/ars.2014.5979

    Acid sphingomyelinase expression is required for cell death and the release of cytochrome c from mitochondria upon infection with Pseudomonas aeruginosa. (A) HL-60 neutrophils or freshly isolated wild-type and Asm-deficient peritoneal neutrophils were infected for 8 h with P. aeruginosa strain ATCC 27853. Apoptosis was determined by staining with FITC-annexin V. Displayed are the means±SD, n =4; * p
    Figure Legend Snippet: Acid sphingomyelinase expression is required for cell death and the release of cytochrome c from mitochondria upon infection with Pseudomonas aeruginosa. (A) HL-60 neutrophils or freshly isolated wild-type and Asm-deficient peritoneal neutrophils were infected for 8 h with P. aeruginosa strain ATCC 27853. Apoptosis was determined by staining with FITC-annexin V. Displayed are the means±SD, n =4; * p

    Techniques Used: Expressing, Infection, Isolation, Staining

    25) Product Images from "Pseudomonas aeruginosa Expresses a Lethal Virulence Determinant, the PA-I Lectin/Adhesin, in the Intestinal Tract of a Stressed Host"

    Article Title: Pseudomonas aeruginosa Expresses a Lethal Virulence Determinant, the PA-I Lectin/Adhesin, in the Intestinal Tract of a Stressed Host

    Journal: Annals of Surgery

    doi: 10.1097/01.sla.0000094551.88143.f8

    FIGURE 1 . Time and dose response curves for the effect of P. aeruginosa on T-84 monolayer transepithelial electrical resistance (TEER). ATCC 27853 ( P. aeruginosa ) was apically inoculated onto cells and TEER determined at the specified intervals. Cumulative mannitol flux across monolayers is also displayed at the specified time interval. * P
    Figure Legend Snippet: FIGURE 1 . Time and dose response curves for the effect of P. aeruginosa on T-84 monolayer transepithelial electrical resistance (TEER). ATCC 27853 ( P. aeruginosa ) was apically inoculated onto cells and TEER determined at the specified intervals. Cumulative mannitol flux across monolayers is also displayed at the specified time interval. * P

    Techniques Used:

    26) Product Images from "Invasion of Human Epithelial Cells by Pseudomonas aeruginosa Involves Src-Like Tyrosine Kinases p60Src and p59Fyn"

    Article Title: Invasion of Human Epithelial Cells by Pseudomonas aeruginosa Involves Src-Like Tyrosine Kinases p60Src and p59Fyn

    Journal: Infection and Immunity

    doi: 10.1128/IAI.69.1.281-287.2001

    Inhibition of P. aeruginosa LPS binding to CFTR prevents activation of p60Src and p59Fyn. Prevention of P. aeruginosa binding to CFTR by incubation with a CFTR peptide encompassing amino acids 103 to 117 blocks activation of Src-like tyrosine kinases (A) and invasion (B) in Chang cells upon infection with the indicated strains. (A) Chang epithelial cells were infected with P. aeruginosa 696 or ATCC 27583 for the indicated times (in minutes) and lysed; then p60Src or p59Fyn was immunoprecipitated and subjected to kinase assays. Chang cells were labeled as above with [ 3 H]choline chloride, the supernatants of the immobilized immunoprecipitates were counted, and the samples were normalized accordingly. P. aeruginosa 696 or ATCC 27853 was incubated with the CFTR peptide 10 min prior to the infection assay. Activities of p60Src and p59Fyn were determined by phosphorylation of enolase as above. An aliquot of the immunoprecipitates was blotted with anti-Src or anti-Fyn, displaying similar amounts of protein in all lanes. Control experiments were performed as above. (B) Chang epithelial cells were infected for 30 min as above with CFTR peptide-treated or untreated P. aeruginosa 696 or ATCC 27583, and invasion was determined by crystal violet staining. Results shown are means ± SD from three independent experiments. Significance was determined by t test, and significant differences are indicated by asterisks.
    Figure Legend Snippet: Inhibition of P. aeruginosa LPS binding to CFTR prevents activation of p60Src and p59Fyn. Prevention of P. aeruginosa binding to CFTR by incubation with a CFTR peptide encompassing amino acids 103 to 117 blocks activation of Src-like tyrosine kinases (A) and invasion (B) in Chang cells upon infection with the indicated strains. (A) Chang epithelial cells were infected with P. aeruginosa 696 or ATCC 27583 for the indicated times (in minutes) and lysed; then p60Src or p59Fyn was immunoprecipitated and subjected to kinase assays. Chang cells were labeled as above with [ 3 H]choline chloride, the supernatants of the immobilized immunoprecipitates were counted, and the samples were normalized accordingly. P. aeruginosa 696 or ATCC 27853 was incubated with the CFTR peptide 10 min prior to the infection assay. Activities of p60Src and p59Fyn were determined by phosphorylation of enolase as above. An aliquot of the immunoprecipitates was blotted with anti-Src or anti-Fyn, displaying similar amounts of protein in all lanes. Control experiments were performed as above. (B) Chang epithelial cells were infected for 30 min as above with CFTR peptide-treated or untreated P. aeruginosa 696 or ATCC 27583, and invasion was determined by crystal violet staining. Results shown are means ± SD from three independent experiments. Significance was determined by t test, and significant differences are indicated by asterisks.

    Techniques Used: Inhibition, Binding Assay, Activation Assay, Incubation, Infection, Immunoprecipitation, Labeling, Staining

    Infection of Chang epithelial cells with P. aeruginosa activates p60Src and p59Fyn. Infection of Chang or WI-38 epithelial cells with P. aeruginosa 696 or ATCC 27853 for the indicated times (in minutes) results in rapid activation of p60Src or p59Fyn. Control immunoprecipitates (up) from infected cells with irrelevant goat sera did not show phosphorylation of the substrate enolase. Likewise, immunoprecipitates (Ipt.) obtained from P. aeruginosa 696 or ATCC 27853 only did not reveal any cross-reactivity. Chang epithelial cells (A) or WI-38 (B) cells were infected with P. aeruginosa 696 or ATCC 27853 for the indicated times in minutes, lysed, and subjected to immunoprecipitation with goat polyclonal anti-Src or anti-Fyn antibodies. Cells were labeled with [ 3 H]choline chloride prior to infection, permitting us to use the supernatants of the agarose-immobilized immunoprecipitates for normalization of the samples prior to the kinase assay. The kinase reaction was initiated by resuspending the immunoprecipitates in kinase buffer supplemented with 10 μg of enolase/ml and 10 μCi of [ 32 P]γ-ATP. The samples were separated by SDS–10% PAGE and analyzed by autoradiography. The activities of p60Src and p59Fyn were determined by phosphorylation of the substrate enolase (P-enolase). Aliquots of the immunoprecipitates were blotted with anti-Fyn or anti-Src, followed by HRP-coupled protein L and enhanced chemiluminescence development, demonstrating similar amounts of Src or Fyn in each experiment. Control experiments were performed by incubation of bacterial lysates from P. aeruginosa 696 or ATCC 27853 with anti-Src or anti-Fyn antisera and reveal that the bacteria do not contain a cross-reacting kinase. Further, lysates from infected cells were subjected to precipitation with an irrelevant antiserum, demonstrating the specificity of the kinase assay.
    Figure Legend Snippet: Infection of Chang epithelial cells with P. aeruginosa activates p60Src and p59Fyn. Infection of Chang or WI-38 epithelial cells with P. aeruginosa 696 or ATCC 27853 for the indicated times (in minutes) results in rapid activation of p60Src or p59Fyn. Control immunoprecipitates (up) from infected cells with irrelevant goat sera did not show phosphorylation of the substrate enolase. Likewise, immunoprecipitates (Ipt.) obtained from P. aeruginosa 696 or ATCC 27853 only did not reveal any cross-reactivity. Chang epithelial cells (A) or WI-38 (B) cells were infected with P. aeruginosa 696 or ATCC 27853 for the indicated times in minutes, lysed, and subjected to immunoprecipitation with goat polyclonal anti-Src or anti-Fyn antibodies. Cells were labeled with [ 3 H]choline chloride prior to infection, permitting us to use the supernatants of the agarose-immobilized immunoprecipitates for normalization of the samples prior to the kinase assay. The kinase reaction was initiated by resuspending the immunoprecipitates in kinase buffer supplemented with 10 μg of enolase/ml and 10 μCi of [ 32 P]γ-ATP. The samples were separated by SDS–10% PAGE and analyzed by autoradiography. The activities of p60Src and p59Fyn were determined by phosphorylation of the substrate enolase (P-enolase). Aliquots of the immunoprecipitates were blotted with anti-Fyn or anti-Src, followed by HRP-coupled protein L and enhanced chemiluminescence development, demonstrating similar amounts of Src or Fyn in each experiment. Control experiments were performed by incubation of bacterial lysates from P. aeruginosa 696 or ATCC 27853 with anti-Src or anti-Fyn antisera and reveal that the bacteria do not contain a cross-reacting kinase. Further, lysates from infected cells were subjected to precipitation with an irrelevant antiserum, demonstrating the specificity of the kinase assay.

    Techniques Used: Infection, Activation Assay, Immunoprecipitation, Labeling, Kinase Assay, Polyacrylamide Gel Electrophoresis, Autoradiography, Incubation

    Internalization of P. aeruginosa into human epithelial cells induces cellular tyrosine phosphorylation. Chang conjunctiva epithelial cells were infected with the invasive P. aeruginosa strain ATCC 27853; then the cells were lysed, and proteins were separated by SDS–10% PAGE and analyzed for tyrosine phosphorylation by Western blotting using the monoclonal anti-phosphotyrosine antibody 4G10. Results reveal a rapid and marked tyrosine phosphorylation of several cellular proteins, in particular those with molecular sizes of 140, 95, 70, 58, and 42 kDa, as soon as 10 min after infection with P. aeruginosa ATCC 27853. Samples were normalized by in vivo labeling of mammalian cells prior to infection and counting aliquots of the lysates.
    Figure Legend Snippet: Internalization of P. aeruginosa into human epithelial cells induces cellular tyrosine phosphorylation. Chang conjunctiva epithelial cells were infected with the invasive P. aeruginosa strain ATCC 27853; then the cells were lysed, and proteins were separated by SDS–10% PAGE and analyzed for tyrosine phosphorylation by Western blotting using the monoclonal anti-phosphotyrosine antibody 4G10. Results reveal a rapid and marked tyrosine phosphorylation of several cellular proteins, in particular those with molecular sizes of 140, 95, 70, 58, and 42 kDa, as soon as 10 min after infection with P. aeruginosa ATCC 27853. Samples were normalized by in vivo labeling of mammalian cells prior to infection and counting aliquots of the lysates.

    Techniques Used: Infection, Polyacrylamide Gel Electrophoresis, Western Blot, In Vivo, Labeling

    27) Product Images from "Antipseudomonal and Immunomodulatory Properties of Esc Peptides: Promising Features for Treatment of Chronic Infectious Diseases and Inflammation"

    Article Title: Antipseudomonal and Immunomodulatory Properties of Esc Peptides: Promising Features for Treatment of Chronic Infectious Diseases and Inflammation

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms22020557

    Effect of Esc(1-21) and its diastereomer Esc(1-21)-1c on Pseudomonas aeruginosa ATCC 27853 infected A549 cells. About 1 × 10 5 cells were seeded in 24-well plates. Upon reaching confluence, they were infected with P. aeruginosa for 2 h and then antibiotic treatment was performed to remove nonadherent extracellular bacteria. Afterwards, infected cells were left untreated or were treated for 1 h with each peptide at different concentrations. ( A ) The percentage of bacteria/cell was calculated with respect to untreated infected samples. All data are the mean from four independent experiments ± standard errors of the means (SEM). ( B ) Light microscopy images of A549 cells at ×10 magnification. Peptide-untreated uninfected cells are control samples (Ctrl).
    Figure Legend Snippet: Effect of Esc(1-21) and its diastereomer Esc(1-21)-1c on Pseudomonas aeruginosa ATCC 27853 infected A549 cells. About 1 × 10 5 cells were seeded in 24-well plates. Upon reaching confluence, they were infected with P. aeruginosa for 2 h and then antibiotic treatment was performed to remove nonadherent extracellular bacteria. Afterwards, infected cells were left untreated or were treated for 1 h with each peptide at different concentrations. ( A ) The percentage of bacteria/cell was calculated with respect to untreated infected samples. All data are the mean from four independent experiments ± standard errors of the means (SEM). ( B ) Light microscopy images of A549 cells at ×10 magnification. Peptide-untreated uninfected cells are control samples (Ctrl).

    Techniques Used: Infection, Light Microscopy

    28) Product Images from "Molecular drug-organiser: Synthesis, characterization and biological evaluation of penicillin V and/or nalidixic acid calixarene-based podands"

    Article Title: Molecular drug-organiser: Synthesis, characterization and biological evaluation of penicillin V and/or nalidixic acid calixarene-based podands

    Journal: Bioorganic & Medicinal Chemistry

    doi: 10.1016/j.bmc.2011.10.031

    Photographs of Petri dishes. Disk/compounds: A = 3 10.0 × 10 −3 g/mL PV/AN– B = 5 5.0 × 10 −3 g/mL AN/AN– C = 4 3.25 × 10 −3 g/mL OH/OH– D = 6 2.53 × 10 −3 g/mL PV/PV. Images: I S. aureus ATCC 25923, II S. aureus ATCC 29213, III E. faecalis ATCC 29212, IV E. coli ATCC 25922, V P. aeruginosa ATCC 27853.
    Figure Legend Snippet: Photographs of Petri dishes. Disk/compounds: A = 3 10.0 × 10 −3 g/mL PV/AN– B = 5 5.0 × 10 −3 g/mL AN/AN– C = 4 3.25 × 10 −3 g/mL OH/OH– D = 6 2.53 × 10 −3 g/mL PV/PV. Images: I S. aureus ATCC 25923, II S. aureus ATCC 29213, III E. faecalis ATCC 29212, IV E. coli ATCC 25922, V P. aeruginosa ATCC 27853.

    Techniques Used:

    Photographs of Petri dishes. Disk/compound: E = NA (1.88 mg/mL) + PVK (2.83 mg/mL)– F = NA (1.88 mg/mL)– G = PVK (2.83 mg/mL). Images: I S. aureus ATCC 25923, II S. aureus ATCC 29213, III E. faecalis ATCC 29212, IV E. coli ATCC 25922, V P. aeruginosa ATCC 27853.
    Figure Legend Snippet: Photographs of Petri dishes. Disk/compound: E = NA (1.88 mg/mL) + PVK (2.83 mg/mL)– F = NA (1.88 mg/mL)– G = PVK (2.83 mg/mL). Images: I S. aureus ATCC 25923, II S. aureus ATCC 29213, III E. faecalis ATCC 29212, IV E. coli ATCC 25922, V P. aeruginosa ATCC 27853.

    Techniques Used:

    Photographs of Petri dishes. Disk/compounds: A = 3 10.0 10 −3 g/mL PV/AN–– I = 3 10.0 × 10 −3 g/mL PV/AN fresh solution– J = PVK 1.25 × 10 −3 g/mL– H = 6 5.8 × 10 −3 g/mL PV/PV. Images: I S. aureus ATCC 25923, II S. aureus ATCC 29213, III E. faecalis ATCC 29212, IV E. coli ATCC 25922, V P. aeruginosa ATCC 27853.
    Figure Legend Snippet: Photographs of Petri dishes. Disk/compounds: A = 3 10.0 10 −3 g/mL PV/AN–– I = 3 10.0 × 10 −3 g/mL PV/AN fresh solution– J = PVK 1.25 × 10 −3 g/mL– H = 6 5.8 × 10 −3 g/mL PV/PV. Images: I S. aureus ATCC 25923, II S. aureus ATCC 29213, III E. faecalis ATCC 29212, IV E. coli ATCC 25922, V P. aeruginosa ATCC 27853.

    Techniques Used:

    29) Product Images from "The Semi-Synthetic Peptide Lin-SB056-1 in Combination with EDTA Exerts Strong Antimicrobial and Antibiofilm Activity against Pseudomonas aeruginosa in Conditions Mimicking Cystic Fibrosis Sputum"

    Article Title: The Semi-Synthetic Peptide Lin-SB056-1 in Combination with EDTA Exerts Strong Antimicrobial and Antibiofilm Activity against Pseudomonas aeruginosa in Conditions Mimicking Cystic Fibrosis Sputum

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms18091994

    Visualization of a BLS under light microscopy at: ( a ) 400×; and ( b ) 1000× magnification, after 72 h of incubation; ( c ) Kinetics of lin-SB056-1 activity, used alone and in combination with EDTA, on the formation of BLSs of P. aeruginosa ATCC 27853 in ASM 80%. Data are reported as mean ± standard error of the mean of three independent experiments. * p
    Figure Legend Snippet: Visualization of a BLS under light microscopy at: ( a ) 400×; and ( b ) 1000× magnification, after 72 h of incubation; ( c ) Kinetics of lin-SB056-1 activity, used alone and in combination with EDTA, on the formation of BLSs of P. aeruginosa ATCC 27853 in ASM 80%. Data are reported as mean ± standard error of the mean of three independent experiments. * p

    Techniques Used: Light Microscopy, Incubation, Activity Assay

    Time kill curves of lin-SB056-1 at its bactericidal concentrations against: ( a ) P. aeruginosa ATCC 27853; and ( b ) P. aeruginosa PaM01 in 10 mM Sodium Phosphate Buffer (SPB) at pH 7.4 supplemented with 1% Tryptone Soya Broth (TSB). The concentrations of lin-SB056-1 (reported in parenthesis) are expressed in μg/mL. Control (CTRL) represents bacteria incubated in the absence of the peptide. Bactericidal activity was defined as a reduction in the numbers of viable bacteria of ≥3-log colony forming units (CFU)/mL at any incubation time tested. The dotted lines represent three-log reduction in CFU number, corresponding to the definition of bactericidal activity. Data are reported as mean ± standard error of the mean of three independent experiments.
    Figure Legend Snippet: Time kill curves of lin-SB056-1 at its bactericidal concentrations against: ( a ) P. aeruginosa ATCC 27853; and ( b ) P. aeruginosa PaM01 in 10 mM Sodium Phosphate Buffer (SPB) at pH 7.4 supplemented with 1% Tryptone Soya Broth (TSB). The concentrations of lin-SB056-1 (reported in parenthesis) are expressed in μg/mL. Control (CTRL) represents bacteria incubated in the absence of the peptide. Bactericidal activity was defined as a reduction in the numbers of viable bacteria of ≥3-log colony forming units (CFU)/mL at any incubation time tested. The dotted lines represent three-log reduction in CFU number, corresponding to the definition of bactericidal activity. Data are reported as mean ± standard error of the mean of three independent experiments.

    Techniques Used: Incubation, Activity Assay

    Activity of lin-SB056-1, used alone and in combination with EDTA, against preformed biofilms of P. aeruginosa : ( a ) ATCC 27853; and ( b ) PaM01 strain. The antibiofilm activity of the peptide, EDTA and peptide-EDTA combination was evaluated by crystal violet staining after 24 h of incubation. The concentrations of lin-SB056-1 and EDTA (reported in parenthesis) are expressed in μg/mL and mM, respectively. Control (CTRL) represents bacteria incubated in the absence of antimicrobial agents; comb: peptide/EDTA combination. Data are reported as mean ± standard error of the mean of three independent experiments. ** p
    Figure Legend Snippet: Activity of lin-SB056-1, used alone and in combination with EDTA, against preformed biofilms of P. aeruginosa : ( a ) ATCC 27853; and ( b ) PaM01 strain. The antibiofilm activity of the peptide, EDTA and peptide-EDTA combination was evaluated by crystal violet staining after 24 h of incubation. The concentrations of lin-SB056-1 and EDTA (reported in parenthesis) are expressed in μg/mL and mM, respectively. Control (CTRL) represents bacteria incubated in the absence of antimicrobial agents; comb: peptide/EDTA combination. Data are reported as mean ± standard error of the mean of three independent experiments. ** p

    Techniques Used: Activity Assay, Staining, Incubation

    Bactericidal activity of lin-SB056-1 used alone and in combination with EDTA against: ( a ) P. aeruginosa ATCC 27853; and ( b ) P. aeruginosa PaM01 strain in ASM 80% after 1.5 h of incubation. The concentrations of lin-SB056-1 and EDTA (reported in parenthesis) are expressed in μg/mL and mM, respectively. Control (CTRL) represents bacteria incubated in the absence of antimicrobial agents; comb: peptide/EDTA combination. Data are reported as mean ± standard error of the mean of three independent experiments. *** p
    Figure Legend Snippet: Bactericidal activity of lin-SB056-1 used alone and in combination with EDTA against: ( a ) P. aeruginosa ATCC 27853; and ( b ) P. aeruginosa PaM01 strain in ASM 80% after 1.5 h of incubation. The concentrations of lin-SB056-1 and EDTA (reported in parenthesis) are expressed in μg/mL and mM, respectively. Control (CTRL) represents bacteria incubated in the absence of antimicrobial agents; comb: peptide/EDTA combination. Data are reported as mean ± standard error of the mean of three independent experiments. *** p

    Techniques Used: Activity Assay, Incubation

    30) Product Images from "Hypoxia Modulates Infection of Epithelial Cells by Pseudomonas aeruginosa"

    Article Title: Hypoxia Modulates Infection of Epithelial Cells by Pseudomonas aeruginosa

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0056491

    Bacterial internalization is decreased in DMOG treated cells. To mimic hypoxia, A549 cells were pre-treated with 1 mM of the pan-hydroxylase inhibitor DMOG or its' solvent DMSO for 24 h and throughout following infection with the P. aeruginosa reference strain ATCC 27853 (A), a CF pathogen B. cenocepacia , J2315 (B) and two clinical P. aeruginosa isolates from CF patients, S7587, mucoid and non-mucoid P. aeruginosa (C and D). Data represent mean ± SEM of 3 independent experiments throughout (* p
    Figure Legend Snippet: Bacterial internalization is decreased in DMOG treated cells. To mimic hypoxia, A549 cells were pre-treated with 1 mM of the pan-hydroxylase inhibitor DMOG or its' solvent DMSO for 24 h and throughout following infection with the P. aeruginosa reference strain ATCC 27853 (A), a CF pathogen B. cenocepacia , J2315 (B) and two clinical P. aeruginosa isolates from CF patients, S7587, mucoid and non-mucoid P. aeruginosa (C and D). Data represent mean ± SEM of 3 independent experiments throughout (* p

    Techniques Used: Infection

    31) Product Images from "Diverse Pathway to Obtain Antibacterial and Antifungal Agents Based on Silica Particles Functionalized by Amino and Phenyl Groups with Cu(II) Ion Complexes"

    Article Title: Diverse Pathway to Obtain Antibacterial and Antifungal Agents Based on Silica Particles Functionalized by Amino and Phenyl Groups with Cu(II) Ion Complexes

    Journal: ACS Omega

    doi: 10.1021/acsomega.0c01335

    Dependence of the SI for P. aeruginosa ATCC 27853 cells on the concentration of the bifunctional silica particle suspensions (purple − control, red − 60 min, green − 120 min).
    Figure Legend Snippet: Dependence of the SI for P. aeruginosa ATCC 27853 cells on the concentration of the bifunctional silica particle suspensions (purple − control, red − 60 min, green − 120 min).

    Techniques Used: Concentration Assay

    32) Product Images from "Prolonged Outbreak of Infection Due to TEM-21-Producing Strains of Pseudomonas aeruginosa and Enterobacteria in a Nursing Home"

    Article Title: Prolonged Outbreak of Infection Due to TEM-21-Producing Strains of Pseudomonas aeruginosa and Enterobacteria in a Nursing Home

    Journal: Journal of Clinical Microbiology

    doi: 10.1128/JCM.43.8.4129-4138.2005

    PFGE profiles of SpeI-digested whole-cell DNA of the 24 P. aeruginosa isolates. Lane M, DNA ladder; lane control, P. aeruginosa ATCC 27853.
    Figure Legend Snippet: PFGE profiles of SpeI-digested whole-cell DNA of the 24 P. aeruginosa isolates. Lane M, DNA ladder; lane control, P. aeruginosa ATCC 27853.

    Techniques Used:

    33) Product Images from "Characterization of an ADP-Ribosyltransferase Toxin (AexT) from Aeromonas salmonicida subsp. salmonicida"

    Article Title: Characterization of an ADP-Ribosyltransferase Toxin (AexT) from Aeromonas salmonicida subsp. salmonicida

    Journal: Journal of Bacteriology

    doi: 10.1128/JB.184.7.1851-1858.2002

    Expression of AexT by A. salmonicida subsp. salmonicida in low-Ca 2+ medium and serological cross-reactions with ExoS and ExoT. Bacterial cultures were grown in Ca 2+ -depleted TSB medium. Lane 1, A. salmonicida subsp. salmonicida wt strain JF2267; lane 2, A. salmonicida subsp. salmonicida aexT mutant JF2580; lane 3, P. aeruginosa strain ATCC 27853. Culture supernatants were concentrated 20-fold and analyzed on immunoblots with anti-AexT antibodies. Lane c, purified recombinant AexT-His as a control; lane st, molecular mass standard. The identity of the band at 30 kDa reacting with P. aeruginosa ATCC 27853 (lane 3) is not determined.
    Figure Legend Snippet: Expression of AexT by A. salmonicida subsp. salmonicida in low-Ca 2+ medium and serological cross-reactions with ExoS and ExoT. Bacterial cultures were grown in Ca 2+ -depleted TSB medium. Lane 1, A. salmonicida subsp. salmonicida wt strain JF2267; lane 2, A. salmonicida subsp. salmonicida aexT mutant JF2580; lane 3, P. aeruginosa strain ATCC 27853. Culture supernatants were concentrated 20-fold and analyzed on immunoblots with anti-AexT antibodies. Lane c, purified recombinant AexT-His as a control; lane st, molecular mass standard. The identity of the band at 30 kDa reacting with P. aeruginosa ATCC 27853 (lane 3) is not determined.

    Techniques Used: Expressing, Mutagenesis, Western Blot, Purification, Recombinant

    34) Product Images from "Interaction of Drug- and Granulocyte-Mediated Killing of Pseudomonas aeruginosa in a Murine Pneumonia Model"

    Article Title: Interaction of Drug- and Granulocyte-Mediated Killing of Pseudomonas aeruginosa in a Murine Pneumonia Model

    Journal: The Journal of Infectious Diseases

    doi: 10.1093/infdis/jiu237

    Colony counts of Pseudomonas aeruginosa ATCC 27853 in the lungs of mice. Cohorts were euthanized at baseline (2 hours after challenge), at hour 26, and at hour 50. The difference between the data for 26 h and 50 h reflects the influence of granulocytes
    Figure Legend Snippet: Colony counts of Pseudomonas aeruginosa ATCC 27853 in the lungs of mice. Cohorts were euthanized at baseline (2 hours after challenge), at hour 26, and at hour 50. The difference between the data for 26 h and 50 h reflects the influence of granulocytes

    Techniques Used: Mouse Assay

    35) Product Images from "In Vitro and In Vivo Assessment of the Efficacy of Bromoageliferin, an Alkaloid Isolated from the Sponge Agelas dilatata, against Pseudomonas aeruginosa"

    Article Title: In Vitro and In Vivo Assessment of the Efficacy of Bromoageliferin, an Alkaloid Isolated from the Sponge Agelas dilatata, against Pseudomonas aeruginosa

    Journal: Marine Drugs

    doi: 10.3390/md18060326

    Quantification of biofilm formation after 24 h by P. aeruginosa strains PAO1 and ATCC 27853 in the presence of different concentrations of bromoageliferin ( 2 ).
    Figure Legend Snippet: Quantification of biofilm formation after 24 h by P. aeruginosa strains PAO1 and ATCC 27853 in the presence of different concentrations of bromoageliferin ( 2 ).

    Techniques Used:

    Survival of G. mellonella larvae ( n = 15 per group) following infection with P. aeruginosa strain ATCC 27853 untreated (PBS) and treated with bromoageliferin ( 2 ) (2 mg/kg).
    Figure Legend Snippet: Survival of G. mellonella larvae ( n = 15 per group) following infection with P. aeruginosa strain ATCC 27853 untreated (PBS) and treated with bromoageliferin ( 2 ) (2 mg/kg).

    Techniques Used: Infection

    Influence of the presence of bromine atoms in A and B pyrrol rings in the antibacterial activity of compounds 1 – 3 against P. aeruginosa ATCC 27853 strain.
    Figure Legend Snippet: Influence of the presence of bromine atoms in A and B pyrrol rings in the antibacterial activity of compounds 1 – 3 against P. aeruginosa ATCC 27853 strain.

    Techniques Used: Activity Assay

    36) Product Images from "Chimeric bacteriocin S5-PmnH engineered by domain swapping efficiently controls Pseudomonas aeruginosa infection in murine keratitis and lung models"

    Article Title: Chimeric bacteriocin S5-PmnH engineered by domain swapping efficiently controls Pseudomonas aeruginosa infection in murine keratitis and lung models

    Journal: Scientific Reports

    doi: 10.1038/s41598-022-09865-8

    Scatterplot of terminal lung burden following IN infection with P. aeruginosa ATCC 27853. The data from the culture burdens were analyzed using appropriate non-parametric statistical models (Kruskal–Wallis using Conover-Inman to make all pairwise comparisons between groups) with StatsDirect (v. 3.3.3). The geometric mean burden of each treatment is indicated by the horizontal bar. *P ≤ 0.05, **P ≤ 0.0005, ***P ≤ 0.0001, compared to vehicle control. LOD limit of detection.
    Figure Legend Snippet: Scatterplot of terminal lung burden following IN infection with P. aeruginosa ATCC 27853. The data from the culture burdens were analyzed using appropriate non-parametric statistical models (Kruskal–Wallis using Conover-Inman to make all pairwise comparisons between groups) with StatsDirect (v. 3.3.3). The geometric mean burden of each treatment is indicated by the horizontal bar. *P ≤ 0.05, **P ≤ 0.0005, ***P ≤ 0.0001, compared to vehicle control. LOD limit of detection.

    Techniques Used: Infection

    37) Product Images from "Synthesis and biological evaluation of new cephalosporin derivatives containing cyclic disulfide moieties"

    Article Title: Synthesis and biological evaluation of new cephalosporin derivatives containing cyclic disulfide moieties

    Journal: bioRxiv

    doi: 10.1101/2021.11.03.467114

    Assessment of biofilm biomass by crystal violet staining of 4 Gram-negative strains:  E. coli  ATCC 25922,  E. coli  K12 MG1622, PAO1,  P. aeruginosa  ATCC 27853. Strains were incubated in eight different media. Error bars represent standard deviation of two independent experiments in triplicate.
    Figure Legend Snippet: Assessment of biofilm biomass by crystal violet staining of 4 Gram-negative strains: E. coli ATCC 25922, E. coli K12 MG1622, PAO1, P. aeruginosa ATCC 27853. Strains were incubated in eight different media. Error bars represent standard deviation of two independent experiments in triplicate.

    Techniques Used: Staining, Incubation, Standard Deviation

    38) Product Images from "The Semi-Synthetic Peptide Lin-SB056-1 in Combination with EDTA Exerts Strong Antimicrobial and Antibiofilm Activity against Pseudomonas aeruginosa in Conditions Mimicking Cystic Fibrosis Sputum"

    Article Title: The Semi-Synthetic Peptide Lin-SB056-1 in Combination with EDTA Exerts Strong Antimicrobial and Antibiofilm Activity against Pseudomonas aeruginosa in Conditions Mimicking Cystic Fibrosis Sputum

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms18091994

    Visualization of a BLS under light microscopy at: ( a ) 400×; and ( b ) 1000× magnification, after 72 h of incubation; ( c ) Kinetics of lin-SB056-1 activity, used alone and in combination with EDTA, on the formation of BLSs of P. aeruginosa ATCC 27853 in ASM 80%. Data are reported as mean ± standard error of the mean of three independent experiments. * p
    Figure Legend Snippet: Visualization of a BLS under light microscopy at: ( a ) 400×; and ( b ) 1000× magnification, after 72 h of incubation; ( c ) Kinetics of lin-SB056-1 activity, used alone and in combination with EDTA, on the formation of BLSs of P. aeruginosa ATCC 27853 in ASM 80%. Data are reported as mean ± standard error of the mean of three independent experiments. * p

    Techniques Used: Light Microscopy, Incubation, Activity Assay

    Time kill curves of lin-SB056-1 at its bactericidal concentrations against: ( a ) P. aeruginosa ATCC 27853; and ( b ) P. aeruginosa PaM01 in 10 mM Sodium Phosphate Buffer (SPB) at pH 7.4 supplemented with 1% Tryptone Soya Broth (TSB). The concentrations of lin-SB056-1 (reported in parenthesis) are expressed in μg/mL. Control (CTRL) represents bacteria incubated in the absence of the peptide. Bactericidal activity was defined as a reduction in the numbers of viable bacteria of ≥3-log colony forming units (CFU)/mL at any incubation time tested. The dotted lines represent three-log reduction in CFU number, corresponding to the definition of bactericidal activity. Data are reported as mean ± standard error of the mean of three independent experiments.
    Figure Legend Snippet: Time kill curves of lin-SB056-1 at its bactericidal concentrations against: ( a ) P. aeruginosa ATCC 27853; and ( b ) P. aeruginosa PaM01 in 10 mM Sodium Phosphate Buffer (SPB) at pH 7.4 supplemented with 1% Tryptone Soya Broth (TSB). The concentrations of lin-SB056-1 (reported in parenthesis) are expressed in μg/mL. Control (CTRL) represents bacteria incubated in the absence of the peptide. Bactericidal activity was defined as a reduction in the numbers of viable bacteria of ≥3-log colony forming units (CFU)/mL at any incubation time tested. The dotted lines represent three-log reduction in CFU number, corresponding to the definition of bactericidal activity. Data are reported as mean ± standard error of the mean of three independent experiments.

    Techniques Used: Incubation, Activity Assay

    Activity of lin-SB056-1, used alone and in combination with EDTA, against preformed biofilms of P. aeruginosa : ( a ) ATCC 27853; and ( b ) PaM01 strain. The antibiofilm activity of the peptide, EDTA and peptide-EDTA combination was evaluated by crystal violet staining after 24 h of incubation. The concentrations of lin-SB056-1 and EDTA (reported in parenthesis) are expressed in μg/mL and mM, respectively. Control (CTRL) represents bacteria incubated in the absence of antimicrobial agents; comb: peptide/EDTA combination. Data are reported as mean ± standard error of the mean of three independent experiments. ** p
    Figure Legend Snippet: Activity of lin-SB056-1, used alone and in combination with EDTA, against preformed biofilms of P. aeruginosa : ( a ) ATCC 27853; and ( b ) PaM01 strain. The antibiofilm activity of the peptide, EDTA and peptide-EDTA combination was evaluated by crystal violet staining after 24 h of incubation. The concentrations of lin-SB056-1 and EDTA (reported in parenthesis) are expressed in μg/mL and mM, respectively. Control (CTRL) represents bacteria incubated in the absence of antimicrobial agents; comb: peptide/EDTA combination. Data are reported as mean ± standard error of the mean of three independent experiments. ** p

    Techniques Used: Activity Assay, Staining, Incubation

    Bactericidal activity of lin-SB056-1 used alone and in combination with EDTA against: ( a ) P. aeruginosa ATCC 27853; and ( b ) P. aeruginosa PaM01 strain in ASM 80% after 1.5 h of incubation. The concentrations of lin-SB056-1 and EDTA (reported in parenthesis) are expressed in μg/mL and mM, respectively. Control (CTRL) represents bacteria incubated in the absence of antimicrobial agents; comb: peptide/EDTA combination. Data are reported as mean ± standard error of the mean of three independent experiments. *** p
    Figure Legend Snippet: Bactericidal activity of lin-SB056-1 used alone and in combination with EDTA against: ( a ) P. aeruginosa ATCC 27853; and ( b ) P. aeruginosa PaM01 strain in ASM 80% after 1.5 h of incubation. The concentrations of lin-SB056-1 and EDTA (reported in parenthesis) are expressed in μg/mL and mM, respectively. Control (CTRL) represents bacteria incubated in the absence of antimicrobial agents; comb: peptide/EDTA combination. Data are reported as mean ± standard error of the mean of three independent experiments. *** p

    Techniques Used: Activity Assay, Incubation

    39) Product Images from "Oxidative responses and defense mechanism of hyperpigmented P. aeruginosa as characterized by proteomics and metabolomics"

    Article Title: Oxidative responses and defense mechanism of hyperpigmented P. aeruginosa as characterized by proteomics and metabolomics

    Journal: EXCLI Journal

    doi: 10.17179/excli2018-1238

    Absorption spectrum of dark-brown containing supernatant of Pseudomonas aeruginosa strain HP (straight line) and non dark-brown containing supernatant of ATCC 27853 (dot line). This measurement displayed a slightly increase in absorption from 700 nm to 430 nm and exponentially increases from 400 nm to 350 nm. The major absorption peak at 357 nm and minor peak (shoulder) at 400 nm were observed in P. aeruginosa HP supernatant, whereas the highest peak of P. aeruginosa ATCC 27853 supernatant was 380 nm with two shoulders at 320 and 400 nm (A). Different adsorption spectra was clearly detected when pigments were extracted and compared to pyocyanin (B).
    Figure Legend Snippet: Absorption spectrum of dark-brown containing supernatant of Pseudomonas aeruginosa strain HP (straight line) and non dark-brown containing supernatant of ATCC 27853 (dot line). This measurement displayed a slightly increase in absorption from 700 nm to 430 nm and exponentially increases from 400 nm to 350 nm. The major absorption peak at 357 nm and minor peak (shoulder) at 400 nm were observed in P. aeruginosa HP supernatant, whereas the highest peak of P. aeruginosa ATCC 27853 supernatant was 380 nm with two shoulders at 320 and 400 nm (A). Different adsorption spectra was clearly detected when pigments were extracted and compared to pyocyanin (B).

    Techniques Used: Adsorption

    2D-PAGE image or the master maps representing protein profiles of P. aeruginosa ATCC 27853 (panels A and C), high pigment produced strain P. aeruginosa HP (panels B and D) in the absence of paraquat (panels A and B) and present of 0.8 mM paraquat (panels C and D) which was separated under pH ranges of 3-10. Numbers of protein spot denoted as identified protein are represented in Table 1.
    Figure Legend Snippet: 2D-PAGE image or the master maps representing protein profiles of P. aeruginosa ATCC 27853 (panels A and C), high pigment produced strain P. aeruginosa HP (panels B and D) in the absence of paraquat (panels A and B) and present of 0.8 mM paraquat (panels C and D) which was separated under pH ranges of 3-10. Numbers of protein spot denoted as identified protein are represented in Table 1.

    Techniques Used: Polyacrylamide Gel Electrophoresis, Produced

    Free radical scavenging activity of extracted dark-brown pigment was determined using NBT assay. The dark-brown pigment extracted from P. aeruginosa shows the anti-oxidant activity effect which exerts its IC 50 at 0.26 mg/ml (B) compared to pigment extracted from ATCC 27853 reference strain which exerts its IC 50 at 0.67 mg/ml (A).
    Figure Legend Snippet: Free radical scavenging activity of extracted dark-brown pigment was determined using NBT assay. The dark-brown pigment extracted from P. aeruginosa shows the anti-oxidant activity effect which exerts its IC 50 at 0.26 mg/ml (B) compared to pigment extracted from ATCC 27853 reference strain which exerts its IC 50 at 0.67 mg/ml (A).

    Techniques Used: Activity Assay

    The comparison of pigment production in LB medium without any supplement from 24 h aerobic cultures of P. aeruginosa ATCC 27853 and P. aeruginosa high pigment clinically isolated strain HP. Brown zone was only observed around the colony of P. aeruginosa HP. Biochemical tests indicated varieties of pigment production based on the substrates utilized. Dark-brown pigment was observed on King P, King F, cetrimide and urea cultured medium but was not observed on glucose containing medium.
    Figure Legend Snippet: The comparison of pigment production in LB medium without any supplement from 24 h aerobic cultures of P. aeruginosa ATCC 27853 and P. aeruginosa high pigment clinically isolated strain HP. Brown zone was only observed around the colony of P. aeruginosa HP. Biochemical tests indicated varieties of pigment production based on the substrates utilized. Dark-brown pigment was observed on King P, King F, cetrimide and urea cultured medium but was not observed on glucose containing medium.

    Techniques Used: Isolation, Cell Culture

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    ATCC p aeruginosa strains 762
    Sphingosine induces a rapid increase in the permeability of P. <t>aeruginosa</t> . A and B, P. aeruginosa ( P.a. ) strains 762 and ATCC 27853 or S. aureus ( S.a. ) strain DH was incubated with 100 n m TO-PRO-3 iodide. Bacteria were then treated for 5 min with 1 or 10 μ m sphingosine ( SPH ) or the corresponding concentration of octylglucopyranoside ( OGP ), the solvent of sphingosine. The bacteria were analyzed by flow cytometry. A shows the quantitative analysis of the means of the fluorescence intensity obtained in the flow cytometry studies (given in arbitrary units; a.u. ), and B shows representative flow cytometry stainings from four independent experiments. Either Triton X-100 or nisin was added as a positive control for membrane permeabilization. C and D , intracellular ATP in P. aeruginosa strains 762 and ATCC 27853 or S. aureus strain DH ( C ) and the release of ATP ( D ) from the bacteria were measured with the BacTiter-Glo reagent. SPH results in a very rapid release of ATP from P. aeruginosa or S. aureus into the supernatant. OGP, the solvent of sphingosine, added at the same concentrations as in the samples with sphingosine, exerted no effect. Either Triton or nisin was added as a positive control for membrane permeabilization and ATP release into the medium. Displayed are the means ± S.D. of four independent experiments each in A , C , and D . ***, p
    P Aeruginosa Strains 762, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC p aeruginosa strains
    Measurements of pyocyanin in P. <t>aeruginosa</t> ATCC 27853 and PAO1 cultured in LB media
    P Aeruginosa Strains, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Sphingosine induces a rapid increase in the permeability of P. aeruginosa . A and B, P. aeruginosa ( P.a. ) strains 762 and ATCC 27853 or S. aureus ( S.a. ) strain DH was incubated with 100 n m TO-PRO-3 iodide. Bacteria were then treated for 5 min with 1 or 10 μ m sphingosine ( SPH ) or the corresponding concentration of octylglucopyranoside ( OGP ), the solvent of sphingosine. The bacteria were analyzed by flow cytometry. A shows the quantitative analysis of the means of the fluorescence intensity obtained in the flow cytometry studies (given in arbitrary units; a.u. ), and B shows representative flow cytometry stainings from four independent experiments. Either Triton X-100 or nisin was added as a positive control for membrane permeabilization. C and D , intracellular ATP in P. aeruginosa strains 762 and ATCC 27853 or S. aureus strain DH ( C ) and the release of ATP ( D ) from the bacteria were measured with the BacTiter-Glo reagent. SPH results in a very rapid release of ATP from P. aeruginosa or S. aureus into the supernatant. OGP, the solvent of sphingosine, added at the same concentrations as in the samples with sphingosine, exerted no effect. Either Triton or nisin was added as a positive control for membrane permeabilization and ATP release into the medium. Displayed are the means ± S.D. of four independent experiments each in A , C , and D . ***, p

    Journal: The Journal of Biological Chemistry

    Article Title: Sphingosine kills bacteria by binding to cardiolipin

    doi: 10.1074/jbc.RA119.012325

    Figure Lengend Snippet: Sphingosine induces a rapid increase in the permeability of P. aeruginosa . A and B, P. aeruginosa ( P.a. ) strains 762 and ATCC 27853 or S. aureus ( S.a. ) strain DH was incubated with 100 n m TO-PRO-3 iodide. Bacteria were then treated for 5 min with 1 or 10 μ m sphingosine ( SPH ) or the corresponding concentration of octylglucopyranoside ( OGP ), the solvent of sphingosine. The bacteria were analyzed by flow cytometry. A shows the quantitative analysis of the means of the fluorescence intensity obtained in the flow cytometry studies (given in arbitrary units; a.u. ), and B shows representative flow cytometry stainings from four independent experiments. Either Triton X-100 or nisin was added as a positive control for membrane permeabilization. C and D , intracellular ATP in P. aeruginosa strains 762 and ATCC 27853 or S. aureus strain DH ( C ) and the release of ATP ( D ) from the bacteria were measured with the BacTiter-Glo reagent. SPH results in a very rapid release of ATP from P. aeruginosa or S. aureus into the supernatant. OGP, the solvent of sphingosine, added at the same concentrations as in the samples with sphingosine, exerted no effect. Either Triton or nisin was added as a positive control for membrane permeabilization and ATP release into the medium. Displayed are the means ± S.D. of four independent experiments each in A , C , and D . ***, p

    Article Snippet: Bacterial killing assays Each of the 10,000 cfu P. aeruginosa strains 762, ATCC 27853, PAO-1, or of the two cardiolipin synthase (Δcls)-deficient PAO-1 mutants or the S. aureus strain DH or 5000 cfu E. coli strains BW25113 or JW1241-5 that were grown to the early logarithmic phase, as described above, was incubated with 1, 5, or 10 μm stearylamine or sphingosine or with the corresponding concentrations of the solvent octylglucopyranoside in H/S or phosphate-buffered saline (PBS) for the indicated times.

    Techniques: Permeability, Incubation, Concentration Assay, Flow Cytometry, Fluorescence, Positive Control

    NH 2 group and protonation of this group are required for the bactericidal effects of sphingosine on P. aeruginosa . A , each of the 10,000 cfu of P. aeruginosa ( P.a. ) strains 762 and ATCC 27853 was incubated with 1, 5, or 10 μ m stearylamine or SPH or 0.0005, 0.0025, or 0.005% of the solvent OGP in PBS adjusted to pH 7.0. After 60 min, the bacteria were washed; aliquots were plated on trypticase soy broth plates, and cfu were counted after growth overnight. Shown are the means ± S.D. of four independent experiments each. ***, p

    Journal: The Journal of Biological Chemistry

    Article Title: Sphingosine kills bacteria by binding to cardiolipin

    doi: 10.1074/jbc.RA119.012325

    Figure Lengend Snippet: NH 2 group and protonation of this group are required for the bactericidal effects of sphingosine on P. aeruginosa . A , each of the 10,000 cfu of P. aeruginosa ( P.a. ) strains 762 and ATCC 27853 was incubated with 1, 5, or 10 μ m stearylamine or SPH or 0.0005, 0.0025, or 0.005% of the solvent OGP in PBS adjusted to pH 7.0. After 60 min, the bacteria were washed; aliquots were plated on trypticase soy broth plates, and cfu were counted after growth overnight. Shown are the means ± S.D. of four independent experiments each. ***, p

    Article Snippet: Bacterial killing assays Each of the 10,000 cfu P. aeruginosa strains 762, ATCC 27853, PAO-1, or of the two cardiolipin synthase (Δcls)-deficient PAO-1 mutants or the S. aureus strain DH or 5000 cfu E. coli strains BW25113 or JW1241-5 that were grown to the early logarithmic phase, as described above, was incubated with 1, 5, or 10 μm stearylamine or sphingosine or with the corresponding concentrations of the solvent octylglucopyranoside in H/S or phosphate-buffered saline (PBS) for the indicated times.

    Techniques: Incubation

    Sphingosine mediates killing of bacteria within minutes. Each of the 10,000 cfu of P. aeruginosa ( P.a. ) strains 762 and ATCC 27853 was incubated in PBS (pH 7.0) with 1 or 10 μ m SPH or the corresponding concentrations of the solvent OGP for 15 or 60 min. Samples were washed, and aliquots were plated on trypticase soy broth plates and were counted after growth overnight. Shown are the means ± S.D. of four independent experiments each. ***, p

    Journal: The Journal of Biological Chemistry

    Article Title: Sphingosine kills bacteria by binding to cardiolipin

    doi: 10.1074/jbc.RA119.012325

    Figure Lengend Snippet: Sphingosine mediates killing of bacteria within minutes. Each of the 10,000 cfu of P. aeruginosa ( P.a. ) strains 762 and ATCC 27853 was incubated in PBS (pH 7.0) with 1 or 10 μ m SPH or the corresponding concentrations of the solvent OGP for 15 or 60 min. Samples were washed, and aliquots were plated on trypticase soy broth plates and were counted after growth overnight. Shown are the means ± S.D. of four independent experiments each. ***, p

    Article Snippet: Bacterial killing assays Each of the 10,000 cfu P. aeruginosa strains 762, ATCC 27853, PAO-1, or of the two cardiolipin synthase (Δcls)-deficient PAO-1 mutants or the S. aureus strain DH or 5000 cfu E. coli strains BW25113 or JW1241-5 that were grown to the early logarithmic phase, as described above, was incubated with 1, 5, or 10 μm stearylamine or sphingosine or with the corresponding concentrations of the solvent octylglucopyranoside in H/S or phosphate-buffered saline (PBS) for the indicated times.

    Techniques: Incubation

    Sphingosine abrogates the metabolic activity of P. aeruginosa and S. aureus . P. aeruginosa ( P.a. ) strains 762 and American Type Culture Collection (ATCC) 27853 or S. aureus ( S.a. ) strain DH was treated with 1 or 10 μ m SPH or OGP, the solvent of sphingosine, at the corresponding concentrations. Samples were then incubated with the oxidized, nonfluorescent form of C 12 -resazurin, and the metabolic activity was determined with flow cytometry by measuring the generation of fluorescent C 12 -resorufin. The studies showed that sphingosine results in a very rapid decrease in bacterial metabolic activity. Either Triton or nisin was added as a positive control for membrane permeabilization and disruption of bacterial metabolism. Fluorescence intensity is given in arbitrary units ( a.u. ). Displayed are the means ± S.D. of four independent experiments each. ***, p

    Journal: The Journal of Biological Chemistry

    Article Title: Sphingosine kills bacteria by binding to cardiolipin

    doi: 10.1074/jbc.RA119.012325

    Figure Lengend Snippet: Sphingosine abrogates the metabolic activity of P. aeruginosa and S. aureus . P. aeruginosa ( P.a. ) strains 762 and American Type Culture Collection (ATCC) 27853 or S. aureus ( S.a. ) strain DH was treated with 1 or 10 μ m SPH or OGP, the solvent of sphingosine, at the corresponding concentrations. Samples were then incubated with the oxidized, nonfluorescent form of C 12 -resazurin, and the metabolic activity was determined with flow cytometry by measuring the generation of fluorescent C 12 -resorufin. The studies showed that sphingosine results in a very rapid decrease in bacterial metabolic activity. Either Triton or nisin was added as a positive control for membrane permeabilization and disruption of bacterial metabolism. Fluorescence intensity is given in arbitrary units ( a.u. ). Displayed are the means ± S.D. of four independent experiments each. ***, p

    Article Snippet: Bacterial killing assays Each of the 10,000 cfu P. aeruginosa strains 762, ATCC 27853, PAO-1, or of the two cardiolipin synthase (Δcls)-deficient PAO-1 mutants or the S. aureus strain DH or 5000 cfu E. coli strains BW25113 or JW1241-5 that were grown to the early logarithmic phase, as described above, was incubated with 1, 5, or 10 μm stearylamine or sphingosine or with the corresponding concentrations of the solvent octylglucopyranoside in H/S or phosphate-buffered saline (PBS) for the indicated times.

    Techniques: Activity Assay, Incubation, Flow Cytometry, Positive Control, Fluorescence

    Time-kill analysis of ( A ) P. aeruginosa and ( B ) E. coli .

    Journal: Molecules

    Article Title: Effects of Essential Oils of Elettaria cardamomum Grown in India and Guatemala on Gram-Negative Bacteria and Gastrointestinal Disorders

    doi: 10.3390/molecules26092546

    Figure Lengend Snippet: Time-kill analysis of ( A ) P. aeruginosa and ( B ) E. coli .

    Article Snippet: Antibacterial Activity The antibacterial effect of the EC-I and EC-G oils was tested against two bacterial strains, namely P. aeruginosa (ATCC 27853) and E. coli (ATCC 35218).

    Techniques:

    SEM analysis performed on P. aeruginosa ATCC 27853. a Untreated; b treated with AMP2041; c holes size measurement

    Journal: Annals of Clinical Microbiology and Antimicrobials

    Article Title: Activity of AMP2041 against human and animal multidrug resistant Pseudomonas aeruginosa clinical isolates

    doi: 10.1186/s12941-017-0193-1

    Figure Lengend Snippet: SEM analysis performed on P. aeruginosa ATCC 27853. a Untreated; b treated with AMP2041; c holes size measurement

    Article Snippet: Scanning electron microscopy analysis The test was performed on the P. aeruginosa ATCC 27853 reference strain.

    Techniques:

    Measurements of pyocyanin in P. aeruginosa ATCC 27853 and PAO1 cultured in LB media

    Journal: BMC Genomics

    Article Title: Comparative genome and transcriptome analysis reveals distinctive surface characteristics and unique physiological potentials of Pseudomonas aeruginosa ATCC 27853

    doi: 10.1186/s12864-017-3842-z

    Figure Lengend Snippet: Measurements of pyocyanin in P. aeruginosa ATCC 27853 and PAO1 cultured in LB media

    Article Snippet: Phylogenetic relationship of the ATCC 27853 with other P. aeruginosa strains based on SNPs from all complete genomes Since the 16S rRNA genes in the different strains of the P. aeruginosa species exhibit high similarity ( > 99%, data not shown) with low discriminating capability, single nucleotide polymorphisms (SNPs) were used to construct the phylogenetic relationship between ATCC 27853 and published strains.

    Techniques: Cell Culture

    Differential expression of the genes involved in the type III and type VI secretion systems and their regulators in P. aeruginosa ATCC 27853 and PAO1. Gene locus tags in PAO1 are shown. Values following gene locus tags of regulators indicate Log2 gene expression changes in PAO1 relative to that in ATCC 27853 ( red color indicates higher expression in PAO1 than in ATCC27853, green color indicates higher expression level in ATCC 27853 than in PAO1). The full list of genes displaying differential expression in the two strains and their values are provided in supplementary Additional file 1 : Table S3

    Journal: BMC Genomics

    Article Title: Comparative genome and transcriptome analysis reveals distinctive surface characteristics and unique physiological potentials of Pseudomonas aeruginosa ATCC 27853

    doi: 10.1186/s12864-017-3842-z

    Figure Lengend Snippet: Differential expression of the genes involved in the type III and type VI secretion systems and their regulators in P. aeruginosa ATCC 27853 and PAO1. Gene locus tags in PAO1 are shown. Values following gene locus tags of regulators indicate Log2 gene expression changes in PAO1 relative to that in ATCC 27853 ( red color indicates higher expression in PAO1 than in ATCC27853, green color indicates higher expression level in ATCC 27853 than in PAO1). The full list of genes displaying differential expression in the two strains and their values are provided in supplementary Additional file 1 : Table S3

    Article Snippet: Phylogenetic relationship of the ATCC 27853 with other P. aeruginosa strains based on SNPs from all complete genomes Since the 16S rRNA genes in the different strains of the P. aeruginosa species exhibit high similarity ( > 99%, data not shown) with low discriminating capability, single nucleotide polymorphisms (SNPs) were used to construct the phylogenetic relationship between ATCC 27853 and published strains.

    Techniques: Expressing

    Colony morphology of P. aeruginosa ATCC 27853 and P. aeruginosa PAO1 cultured at 25 °C on LB agar plates supplemented with Congo Red

    Journal: BMC Genomics

    Article Title: Comparative genome and transcriptome analysis reveals distinctive surface characteristics and unique physiological potentials of Pseudomonas aeruginosa ATCC 27853

    doi: 10.1186/s12864-017-3842-z

    Figure Lengend Snippet: Colony morphology of P. aeruginosa ATCC 27853 and P. aeruginosa PAO1 cultured at 25 °C on LB agar plates supplemented with Congo Red

    Article Snippet: Phylogenetic relationship of the ATCC 27853 with other P. aeruginosa strains based on SNPs from all complete genomes Since the 16S rRNA genes in the different strains of the P. aeruginosa species exhibit high similarity ( > 99%, data not shown) with low discriminating capability, single nucleotide polymorphisms (SNPs) were used to construct the phylogenetic relationship between ATCC 27853 and published strains.

    Techniques: Cell Culture

    The genome wide transcriptomic profile of P. aeruginosa ATCC 27853 and PAO1. Green dots represent genes with higher relative expression level in PAO1 and red dots indicate genes with higher relative expression levels in ATCC 27853. The blue dashed lines represent log 2 -fold changes in expression. Selective genes and operons with distinctive expression patterns in the two strains are indicated

    Journal: BMC Genomics

    Article Title: Comparative genome and transcriptome analysis reveals distinctive surface characteristics and unique physiological potentials of Pseudomonas aeruginosa ATCC 27853

    doi: 10.1186/s12864-017-3842-z

    Figure Lengend Snippet: The genome wide transcriptomic profile of P. aeruginosa ATCC 27853 and PAO1. Green dots represent genes with higher relative expression level in PAO1 and red dots indicate genes with higher relative expression levels in ATCC 27853. The blue dashed lines represent log 2 -fold changes in expression. Selective genes and operons with distinctive expression patterns in the two strains are indicated

    Article Snippet: Phylogenetic relationship of the ATCC 27853 with other P. aeruginosa strains based on SNPs from all complete genomes Since the 16S rRNA genes in the different strains of the P. aeruginosa species exhibit high similarity ( > 99%, data not shown) with low discriminating capability, single nucleotide polymorphisms (SNPs) were used to construct the phylogenetic relationship between ATCC 27853 and published strains.

    Techniques: Genome Wide, Expressing

    Phylogenetic relationships of the currently available 59 complete genomes of Pseudomonas aeruginosa constructed based on the SNPs identified using Harvest with 100 bootstrap and maximum likelihood (ML) criterion in MEGA software. P. aeruginosa ATCC 27853 is highlighted in blue and italic style. The denotation of the strain is listed in the Additional file 1 : Table S1

    Journal: BMC Genomics

    Article Title: Comparative genome and transcriptome analysis reveals distinctive surface characteristics and unique physiological potentials of Pseudomonas aeruginosa ATCC 27853

    doi: 10.1186/s12864-017-3842-z

    Figure Lengend Snippet: Phylogenetic relationships of the currently available 59 complete genomes of Pseudomonas aeruginosa constructed based on the SNPs identified using Harvest with 100 bootstrap and maximum likelihood (ML) criterion in MEGA software. P. aeruginosa ATCC 27853 is highlighted in blue and italic style. The denotation of the strain is listed in the Additional file 1 : Table S1

    Article Snippet: Phylogenetic relationship of the ATCC 27853 with other P. aeruginosa strains based on SNPs from all complete genomes Since the 16S rRNA genes in the different strains of the P. aeruginosa species exhibit high similarity ( > 99%, data not shown) with low discriminating capability, single nucleotide polymorphisms (SNPs) were used to construct the phylogenetic relationship between ATCC 27853 and published strains.

    Techniques: Construct, Software

    Venn diagram showing the number of shared and exclusive genes among four P. aeruginosa strains: P. aeruginosa ATCC 27853, P. aeruginosa PAO1, P. aeruginosa PA14, P. aeruginosa LESB58. The number of unique genes, those shared among two, three and all four strains of ATCC 27853, PAO1, PA14 and LESB58 strains based on the COG gene annotations are shown

    Journal: BMC Genomics

    Article Title: Comparative genome and transcriptome analysis reveals distinctive surface characteristics and unique physiological potentials of Pseudomonas aeruginosa ATCC 27853

    doi: 10.1186/s12864-017-3842-z

    Figure Lengend Snippet: Venn diagram showing the number of shared and exclusive genes among four P. aeruginosa strains: P. aeruginosa ATCC 27853, P. aeruginosa PAO1, P. aeruginosa PA14, P. aeruginosa LESB58. The number of unique genes, those shared among two, three and all four strains of ATCC 27853, PAO1, PA14 and LESB58 strains based on the COG gene annotations are shown

    Article Snippet: Phylogenetic relationship of the ATCC 27853 with other P. aeruginosa strains based on SNPs from all complete genomes Since the 16S rRNA genes in the different strains of the P. aeruginosa species exhibit high similarity ( > 99%, data not shown) with low discriminating capability, single nucleotide polymorphisms (SNPs) were used to construct the phylogenetic relationship between ATCC 27853 and published strains.

    Techniques:

    Comparison of the gene cluster of phenazine biosynthesis (Phz1) and its flanking regions in three strains of P. aeruginosa : ATCC 27853, PAO1 and PA14. Genomic location of the prophage 2 upstream of phz1 gene cluster in ATCC 27853 is highlighted

    Journal: BMC Genomics

    Article Title: Comparative genome and transcriptome analysis reveals distinctive surface characteristics and unique physiological potentials of Pseudomonas aeruginosa ATCC 27853

    doi: 10.1186/s12864-017-3842-z

    Figure Lengend Snippet: Comparison of the gene cluster of phenazine biosynthesis (Phz1) and its flanking regions in three strains of P. aeruginosa : ATCC 27853, PAO1 and PA14. Genomic location of the prophage 2 upstream of phz1 gene cluster in ATCC 27853 is highlighted

    Article Snippet: Phylogenetic relationship of the ATCC 27853 with other P. aeruginosa strains based on SNPs from all complete genomes Since the 16S rRNA genes in the different strains of the P. aeruginosa species exhibit high similarity ( > 99%, data not shown) with low discriminating capability, single nucleotide polymorphisms (SNPs) were used to construct the phylogenetic relationship between ATCC 27853 and published strains.

    Techniques:

    Circular genome map of P. aeruginosa ATCC 27853 showing the Genomic Islands (GIs) predicted by IslandViewer and prophages. From the outside: circles 1 and 2 (clockwise and counterclockwise) genes on the + and - strands, respectively; circles 3, prophages; 4, Genomic Islands; 5, PAO1 genes; 6, GC content; 7, GC skew. The scale in mbp is indicated on the innermost of the map

    Journal: BMC Genomics

    Article Title: Comparative genome and transcriptome analysis reveals distinctive surface characteristics and unique physiological potentials of Pseudomonas aeruginosa ATCC 27853

    doi: 10.1186/s12864-017-3842-z

    Figure Lengend Snippet: Circular genome map of P. aeruginosa ATCC 27853 showing the Genomic Islands (GIs) predicted by IslandViewer and prophages. From the outside: circles 1 and 2 (clockwise and counterclockwise) genes on the + and - strands, respectively; circles 3, prophages; 4, Genomic Islands; 5, PAO1 genes; 6, GC content; 7, GC skew. The scale in mbp is indicated on the innermost of the map

    Article Snippet: Phylogenetic relationship of the ATCC 27853 with other P. aeruginosa strains based on SNPs from all complete genomes Since the 16S rRNA genes in the different strains of the P. aeruginosa species exhibit high similarity ( > 99%, data not shown) with low discriminating capability, single nucleotide polymorphisms (SNPs) were used to construct the phylogenetic relationship between ATCC 27853 and published strains.

    Techniques: