p acanthamoebae bn 9  (ATCC)


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    Structured Review

    ATCC p acanthamoebae bn 9
    Detection and analysis of P. <t>acanthamoebae</t> DNA in smear samples obtained from a hospital environment. (A) Representative results of agarose gel electrophoresis of P. acanthamoebae PCR products amplified from smear samples. (B) Alignment analysis of four P. acanthamoebae amplicons (samples 5, 8, 9, and 51) with previously reported Parachlamydiaceae sequences was performed using Genetyx-Mac software (version 10.1). Asterisks represent conserved sequences in all P. acanthamoebae PCR amplicons. Dots and unmarked positions represent base mismatches and additional bases in the alignment, respectively. P. amoebophila, Protochlamydia amoebophila; N. hartmannellae, Neochlamydia hartmannellae. (C) Construction of a phylogenetic tree for P. acanthamoebae amplicons (samples 5, 8, 9, and 51) with previously reported Parachlamydiaceae sequences was performed using the neighbor-joining method in MEGA software (version 4). Strain names are in parentheses. The accession numbers assigned by DDBJ were as follows: sample 5, uncultured Parachlamydia sp. HUHP-5, AB565471; sample 8, HUHP-8, AB565472; sample 9, HUHP-9, AB565473; sample 51, HUHP-51, AB565474.
    P Acanthamoebae Bn 9, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p acanthamoebae bn 9/product/ATCC
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    p acanthamoebae bn 9 - by Bioz Stars, 2024-04
    93/100 stars

    Images

    1) Product Images from "Impact of Free-Living Amoebae on Presence of Parachlamydia acanthamoebae in the Hospital Environment and Its Survival In Vitro without Requirement for Amoebae"

    Article Title: Impact of Free-Living Amoebae on Presence of Parachlamydia acanthamoebae in the Hospital Environment and Its Survival In Vitro without Requirement for Amoebae

    Journal: Journal of Clinical Microbiology

    doi: 10.1128/JCM.00366-10

    Detection and analysis of P. acanthamoebae DNA in smear samples obtained from a hospital environment. (A) Representative results of agarose gel electrophoresis of P. acanthamoebae PCR products amplified from smear samples. (B) Alignment analysis of four P. acanthamoebae amplicons (samples 5, 8, 9, and 51) with previously reported Parachlamydiaceae sequences was performed using Genetyx-Mac software (version 10.1). Asterisks represent conserved sequences in all P. acanthamoebae PCR amplicons. Dots and unmarked positions represent base mismatches and additional bases in the alignment, respectively. P. amoebophila, Protochlamydia amoebophila; N. hartmannellae, Neochlamydia hartmannellae. (C) Construction of a phylogenetic tree for P. acanthamoebae amplicons (samples 5, 8, 9, and 51) with previously reported Parachlamydiaceae sequences was performed using the neighbor-joining method in MEGA software (version 4). Strain names are in parentheses. The accession numbers assigned by DDBJ were as follows: sample 5, uncultured Parachlamydia sp. HUHP-5, AB565471; sample 8, HUHP-8, AB565472; sample 9, HUHP-9, AB565473; sample 51, HUHP-51, AB565474.
    Figure Legend Snippet: Detection and analysis of P. acanthamoebae DNA in smear samples obtained from a hospital environment. (A) Representative results of agarose gel electrophoresis of P. acanthamoebae PCR products amplified from smear samples. (B) Alignment analysis of four P. acanthamoebae amplicons (samples 5, 8, 9, and 51) with previously reported Parachlamydiaceae sequences was performed using Genetyx-Mac software (version 10.1). Asterisks represent conserved sequences in all P. acanthamoebae PCR amplicons. Dots and unmarked positions represent base mismatches and additional bases in the alignment, respectively. P. amoebophila, Protochlamydia amoebophila; N. hartmannellae, Neochlamydia hartmannellae. (C) Construction of a phylogenetic tree for P. acanthamoebae amplicons (samples 5, 8, 9, and 51) with previously reported Parachlamydiaceae sequences was performed using the neighbor-joining method in MEGA software (version 4). Strain names are in parentheses. The accession numbers assigned by DDBJ were as follows: sample 5, uncultured Parachlamydia sp. HUHP-5, AB565471; sample 8, HUHP-8, AB565472; sample 9, HUHP-9, AB565473; sample 51, HUHP-51, AB565474.

    Techniques Used: Agarose Gel Electrophoresis, Amplification, Software

    Correlation between prevalence of P.  acanthamoebae  and that of Acanthamoeba spp. in the hospital environment
    Figure Legend Snippet: Correlation between prevalence of P. acanthamoebae and that of Acanthamoeba spp. in the hospital environment

    Techniques Used:

    Influence of floor level on the prevalences of P. acanthamoebae (A) and Acanthamoeba (B). Asterisks indicate statistical significance.
    Figure Legend Snippet: Influence of floor level on the prevalences of P. acanthamoebae (A) and Acanthamoeba (B). Asterisks indicate statistical significance.

    Techniques Used:

    Representative images of Live/Dead staining in P. acanthamoebae (A) and numbers of P. acanthamoebae infective progeny in cultures without amoebae (B). (A) The bacteria at 28 days after incubation were stained with a Live/Dead staining kit. Green, membrane integrity stable (possibly viable bacteria); red, dead bacteria. Magnification, ×400. (B) A bacterial solution of 100 μl containing approximately 107 to 108 AIU prepared with PAS was inoculated into the wells of a 24-well plate with (moist conditions) or without (dry conditions) 900 μl of PAS and incubated for up to 28 days at 15°C or 30°C in a normal atmosphere. The numbers of infective progeny as a marker of bacterial viability in the solution were determined using the AIU assay. The data represent the average AIU counts ± standard deviations. *, P < 0.05.
    Figure Legend Snippet: Representative images of Live/Dead staining in P. acanthamoebae (A) and numbers of P. acanthamoebae infective progeny in cultures without amoebae (B). (A) The bacteria at 28 days after incubation were stained with a Live/Dead staining kit. Green, membrane integrity stable (possibly viable bacteria); red, dead bacteria. Magnification, ×400. (B) A bacterial solution of 100 μl containing approximately 107 to 108 AIU prepared with PAS was inoculated into the wells of a 24-well plate with (moist conditions) or without (dry conditions) 900 μl of PAS and incubated for up to 28 days at 15°C or 30°C in a normal atmosphere. The numbers of infective progeny as a marker of bacterial viability in the solution were determined using the AIU assay. The data represent the average AIU counts ± standard deviations. *, P < 0.05.

    Techniques Used: Staining, Incubation, Marker

    p acanthamoebae bn 9  (ATCC)


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    Structured Review

    ATCC p acanthamoebae bn 9
    Detection and analysis of P. <t>acanthamoebae</t> DNA in smear samples obtained from a hospital environment. (A) Representative results of agarose gel electrophoresis of P. acanthamoebae PCR products amplified from smear samples. (B) Alignment analysis of four P. acanthamoebae amplicons (samples 5, 8, 9, and 51) with previously reported Parachlamydiaceae sequences was performed using Genetyx-Mac software (version 10.1). Asterisks represent conserved sequences in all P. acanthamoebae PCR amplicons. Dots and unmarked positions represent base mismatches and additional bases in the alignment, respectively. P. amoebophila, Protochlamydia amoebophila; N. hartmannellae, Neochlamydia hartmannellae. (C) Construction of a phylogenetic tree for P. acanthamoebae amplicons (samples 5, 8, 9, and 51) with previously reported Parachlamydiaceae sequences was performed using the neighbor-joining method in MEGA software (version 4). Strain names are in parentheses. The accession numbers assigned by DDBJ were as follows: sample 5, uncultured Parachlamydia sp. HUHP-5, AB565471; sample 8, HUHP-8, AB565472; sample 9, HUHP-9, AB565473; sample 51, HUHP-51, AB565474.
    P Acanthamoebae Bn 9, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p acanthamoebae bn 9/product/ATCC
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    p acanthamoebae bn 9 - by Bioz Stars, 2024-04
    93/100 stars

    Images

    1) Product Images from "Impact of Free-Living Amoebae on Presence of Parachlamydia acanthamoebae in the Hospital Environment and Its Survival In Vitro without Requirement for Amoebae"

    Article Title: Impact of Free-Living Amoebae on Presence of Parachlamydia acanthamoebae in the Hospital Environment and Its Survival In Vitro without Requirement for Amoebae

    Journal: Journal of Clinical Microbiology

    doi: 10.1128/JCM.00366-10

    Detection and analysis of P. acanthamoebae DNA in smear samples obtained from a hospital environment. (A) Representative results of agarose gel electrophoresis of P. acanthamoebae PCR products amplified from smear samples. (B) Alignment analysis of four P. acanthamoebae amplicons (samples 5, 8, 9, and 51) with previously reported Parachlamydiaceae sequences was performed using Genetyx-Mac software (version 10.1). Asterisks represent conserved sequences in all P. acanthamoebae PCR amplicons. Dots and unmarked positions represent base mismatches and additional bases in the alignment, respectively. P. amoebophila, Protochlamydia amoebophila; N. hartmannellae, Neochlamydia hartmannellae. (C) Construction of a phylogenetic tree for P. acanthamoebae amplicons (samples 5, 8, 9, and 51) with previously reported Parachlamydiaceae sequences was performed using the neighbor-joining method in MEGA software (version 4). Strain names are in parentheses. The accession numbers assigned by DDBJ were as follows: sample 5, uncultured Parachlamydia sp. HUHP-5, AB565471; sample 8, HUHP-8, AB565472; sample 9, HUHP-9, AB565473; sample 51, HUHP-51, AB565474.
    Figure Legend Snippet: Detection and analysis of P. acanthamoebae DNA in smear samples obtained from a hospital environment. (A) Representative results of agarose gel electrophoresis of P. acanthamoebae PCR products amplified from smear samples. (B) Alignment analysis of four P. acanthamoebae amplicons (samples 5, 8, 9, and 51) with previously reported Parachlamydiaceae sequences was performed using Genetyx-Mac software (version 10.1). Asterisks represent conserved sequences in all P. acanthamoebae PCR amplicons. Dots and unmarked positions represent base mismatches and additional bases in the alignment, respectively. P. amoebophila, Protochlamydia amoebophila; N. hartmannellae, Neochlamydia hartmannellae. (C) Construction of a phylogenetic tree for P. acanthamoebae amplicons (samples 5, 8, 9, and 51) with previously reported Parachlamydiaceae sequences was performed using the neighbor-joining method in MEGA software (version 4). Strain names are in parentheses. The accession numbers assigned by DDBJ were as follows: sample 5, uncultured Parachlamydia sp. HUHP-5, AB565471; sample 8, HUHP-8, AB565472; sample 9, HUHP-9, AB565473; sample 51, HUHP-51, AB565474.

    Techniques Used: Agarose Gel Electrophoresis, Amplification, Software

    Correlation between prevalence of P.  acanthamoebae  and that of Acanthamoeba spp. in the hospital environment
    Figure Legend Snippet: Correlation between prevalence of P. acanthamoebae and that of Acanthamoeba spp. in the hospital environment

    Techniques Used:

    Influence of floor level on the prevalences of P. acanthamoebae (A) and Acanthamoeba (B). Asterisks indicate statistical significance.
    Figure Legend Snippet: Influence of floor level on the prevalences of P. acanthamoebae (A) and Acanthamoeba (B). Asterisks indicate statistical significance.

    Techniques Used:

    Representative images of Live/Dead staining in P. acanthamoebae (A) and numbers of P. acanthamoebae infective progeny in cultures without amoebae (B). (A) The bacteria at 28 days after incubation were stained with a Live/Dead staining kit. Green, membrane integrity stable (possibly viable bacteria); red, dead bacteria. Magnification, ×400. (B) A bacterial solution of 100 μl containing approximately 107 to 108 AIU prepared with PAS was inoculated into the wells of a 24-well plate with (moist conditions) or without (dry conditions) 900 μl of PAS and incubated for up to 28 days at 15°C or 30°C in a normal atmosphere. The numbers of infective progeny as a marker of bacterial viability in the solution were determined using the AIU assay. The data represent the average AIU counts ± standard deviations. *, P < 0.05.
    Figure Legend Snippet: Representative images of Live/Dead staining in P. acanthamoebae (A) and numbers of P. acanthamoebae infective progeny in cultures without amoebae (B). (A) The bacteria at 28 days after incubation were stained with a Live/Dead staining kit. Green, membrane integrity stable (possibly viable bacteria); red, dead bacteria. Magnification, ×400. (B) A bacterial solution of 100 μl containing approximately 107 to 108 AIU prepared with PAS was inoculated into the wells of a 24-well plate with (moist conditions) or without (dry conditions) 900 μl of PAS and incubated for up to 28 days at 15°C or 30°C in a normal atmosphere. The numbers of infective progeny as a marker of bacterial viability in the solution were determined using the AIU assay. The data represent the average AIU counts ± standard deviations. *, P < 0.05.

    Techniques Used: Staining, Incubation, Marker

    p acanthamoebae bn 9  (ATCC)


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    ATCC p acanthamoebae bn 9
    P Acanthamoebae Bn 9, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p acanthamoebae bn 9/product/ATCC
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    p acanthamoebae bn 9 - by Bioz Stars, 2024-04
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    p acanthamoebae bn 9  (ATCC)


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  • 93

    Structured Review

    ATCC p acanthamoebae bn 9
    P Acanthamoebae Bn 9, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p acanthamoebae bn 9/product/ATCC
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    p acanthamoebae bn 9 - by Bioz Stars, 2024-04
    93/100 stars

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    ATCC p acanthamoebae bn 9
    Detection and analysis of P. <t>acanthamoebae</t> DNA in smear samples obtained from a hospital environment. (A) Representative results of agarose gel electrophoresis of P. acanthamoebae PCR products amplified from smear samples. (B) Alignment analysis of four P. acanthamoebae amplicons (samples 5, 8, 9, and 51) with previously reported Parachlamydiaceae sequences was performed using Genetyx-Mac software (version 10.1). Asterisks represent conserved sequences in all P. acanthamoebae PCR amplicons. Dots and unmarked positions represent base mismatches and additional bases in the alignment, respectively. P. amoebophila, Protochlamydia amoebophila; N. hartmannellae, Neochlamydia hartmannellae. (C) Construction of a phylogenetic tree for P. acanthamoebae amplicons (samples 5, 8, 9, and 51) with previously reported Parachlamydiaceae sequences was performed using the neighbor-joining method in MEGA software (version 4). Strain names are in parentheses. The accession numbers assigned by DDBJ were as follows: sample 5, uncultured Parachlamydia sp. HUHP-5, AB565471; sample 8, HUHP-8, AB565472; sample 9, HUHP-9, AB565473; sample 51, HUHP-51, AB565474.
    P Acanthamoebae Bn 9, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p acanthamoebae bn 9/product/ATCC
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    p acanthamoebae bn 9 - by Bioz Stars, 2024-04
    93/100 stars
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    Detection and analysis of P. acanthamoebae DNA in smear samples obtained from a hospital environment. (A) Representative results of agarose gel electrophoresis of P. acanthamoebae PCR products amplified from smear samples. (B) Alignment analysis of four P. acanthamoebae amplicons (samples 5, 8, 9, and 51) with previously reported Parachlamydiaceae sequences was performed using Genetyx-Mac software (version 10.1). Asterisks represent conserved sequences in all P. acanthamoebae PCR amplicons. Dots and unmarked positions represent base mismatches and additional bases in the alignment, respectively. P. amoebophila, Protochlamydia amoebophila; N. hartmannellae, Neochlamydia hartmannellae. (C) Construction of a phylogenetic tree for P. acanthamoebae amplicons (samples 5, 8, 9, and 51) with previously reported Parachlamydiaceae sequences was performed using the neighbor-joining method in MEGA software (version 4). Strain names are in parentheses. The accession numbers assigned by DDBJ were as follows: sample 5, uncultured Parachlamydia sp. HUHP-5, AB565471; sample 8, HUHP-8, AB565472; sample 9, HUHP-9, AB565473; sample 51, HUHP-51, AB565474.

    Journal: Journal of Clinical Microbiology

    Article Title: Impact of Free-Living Amoebae on Presence of Parachlamydia acanthamoebae in the Hospital Environment and Its Survival In Vitro without Requirement for Amoebae

    doi: 10.1128/JCM.00366-10

    Figure Lengend Snippet: Detection and analysis of P. acanthamoebae DNA in smear samples obtained from a hospital environment. (A) Representative results of agarose gel electrophoresis of P. acanthamoebae PCR products amplified from smear samples. (B) Alignment analysis of four P. acanthamoebae amplicons (samples 5, 8, 9, and 51) with previously reported Parachlamydiaceae sequences was performed using Genetyx-Mac software (version 10.1). Asterisks represent conserved sequences in all P. acanthamoebae PCR amplicons. Dots and unmarked positions represent base mismatches and additional bases in the alignment, respectively. P. amoebophila, Protochlamydia amoebophila; N. hartmannellae, Neochlamydia hartmannellae. (C) Construction of a phylogenetic tree for P. acanthamoebae amplicons (samples 5, 8, 9, and 51) with previously reported Parachlamydiaceae sequences was performed using the neighbor-joining method in MEGA software (version 4). Strain names are in parentheses. The accession numbers assigned by DDBJ were as follows: sample 5, uncultured Parachlamydia sp. HUHP-5, AB565471; sample 8, HUHP-8, AB565472; sample 9, HUHP-9, AB565473; sample 51, HUHP-51, AB565474.

    Article Snippet: P. acanthamoebae Bn 9 (ATCC VR-1476) was purchased from the American Type Culture Collection.

    Techniques: Agarose Gel Electrophoresis, Amplification, Software

    Correlation between prevalence of P.  acanthamoebae  and that of Acanthamoeba spp. in the hospital environment

    Journal: Journal of Clinical Microbiology

    Article Title: Impact of Free-Living Amoebae on Presence of Parachlamydia acanthamoebae in the Hospital Environment and Its Survival In Vitro without Requirement for Amoebae

    doi: 10.1128/JCM.00366-10

    Figure Lengend Snippet: Correlation between prevalence of P. acanthamoebae and that of Acanthamoeba spp. in the hospital environment

    Article Snippet: P. acanthamoebae Bn 9 (ATCC VR-1476) was purchased from the American Type Culture Collection.

    Techniques:

    Influence of floor level on the prevalences of P. acanthamoebae (A) and Acanthamoeba (B). Asterisks indicate statistical significance.

    Journal: Journal of Clinical Microbiology

    Article Title: Impact of Free-Living Amoebae on Presence of Parachlamydia acanthamoebae in the Hospital Environment and Its Survival In Vitro without Requirement for Amoebae

    doi: 10.1128/JCM.00366-10

    Figure Lengend Snippet: Influence of floor level on the prevalences of P. acanthamoebae (A) and Acanthamoeba (B). Asterisks indicate statistical significance.

    Article Snippet: P. acanthamoebae Bn 9 (ATCC VR-1476) was purchased from the American Type Culture Collection.

    Techniques:

    Representative images of Live/Dead staining in P. acanthamoebae (A) and numbers of P. acanthamoebae infective progeny in cultures without amoebae (B). (A) The bacteria at 28 days after incubation were stained with a Live/Dead staining kit. Green, membrane integrity stable (possibly viable bacteria); red, dead bacteria. Magnification, ×400. (B) A bacterial solution of 100 μl containing approximately 107 to 108 AIU prepared with PAS was inoculated into the wells of a 24-well plate with (moist conditions) or without (dry conditions) 900 μl of PAS and incubated for up to 28 days at 15°C or 30°C in a normal atmosphere. The numbers of infective progeny as a marker of bacterial viability in the solution were determined using the AIU assay. The data represent the average AIU counts ± standard deviations. *, P < 0.05.

    Journal: Journal of Clinical Microbiology

    Article Title: Impact of Free-Living Amoebae on Presence of Parachlamydia acanthamoebae in the Hospital Environment and Its Survival In Vitro without Requirement for Amoebae

    doi: 10.1128/JCM.00366-10

    Figure Lengend Snippet: Representative images of Live/Dead staining in P. acanthamoebae (A) and numbers of P. acanthamoebae infective progeny in cultures without amoebae (B). (A) The bacteria at 28 days after incubation were stained with a Live/Dead staining kit. Green, membrane integrity stable (possibly viable bacteria); red, dead bacteria. Magnification, ×400. (B) A bacterial solution of 100 μl containing approximately 107 to 108 AIU prepared with PAS was inoculated into the wells of a 24-well plate with (moist conditions) or without (dry conditions) 900 μl of PAS and incubated for up to 28 days at 15°C or 30°C in a normal atmosphere. The numbers of infective progeny as a marker of bacterial viability in the solution were determined using the AIU assay. The data represent the average AIU counts ± standard deviations. *, P < 0.05.

    Article Snippet: P. acanthamoebae Bn 9 (ATCC VR-1476) was purchased from the American Type Culture Collection.

    Techniques: Staining, Incubation, Marker