otoferlin cdna fragments  (Thermo Fisher)


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    Structured Review

    Thermo Fisher otoferlin cdna fragments
    <t>Otoferlin</t> dual‐ AAV ‐ TS ‐transduced Otof −/− organs of Corti express full‐length otoferlin mRNA Schematic representation of otoferlin <t>cDNA</t> from otoferlin dual‐AAV‐transduced, wild‐type, and Otof −/− organs of Corti, displaying binding sites of primers used in PCRs to assess dual‐AAV reassembly. Otoferlin PCR amplicons from organ of Corti cDNA. A 1,753‐bp‐long amplicon (*), also present in non‐injected wild‐type controls (WTB6, WTCD1B6F1), indicates successful reassembly of the split otoferlin expression cassette in otoferlin dual‐AAV‐TS‐transduced CD1B6F1‐ Otof −/− organs of Corti (injected ear). In Otof −/− samples, three shorter products were amplified (a, b and c). Sanger sequencing confirmed correct dual‐AAV split‐site assembly (dashed line) as well as the presence of an artificial AccIII restriction site introduced in the dual‐AAV‐TS otoferlin cDNA, which is absent in the wild‐type (WT) and Otof −/− cDNA (a–c). Amplicons a‐c from Otof −/− organs of Corti all lack exons 14–15, while bands “b” (1,480 bp) and “c” (1,679 bp) still contain intron 20–21 (b) or intron 23–24 (c), respectively. Western blotting on cell lysates of WT and Otof −/− CD1B6F1 organs of Corti. Two bands of ˜210–230 kDa, corresponding to full‐length otoferlin, were detected in WT but absent in Otof −/− ears. (**) refers to an unspecific band detected in both samples. GAPDH was used as loading control. Data information: CDS: coding sequence, Ex: exon, TS: trans‐splicing, Hyb: hybrid, control ear: non‐treated ears, non‐injected ear: contralateral non‐injected Otof −/− ears. Source data are available online for this figure.
    Otoferlin Cdna Fragments, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/otoferlin cdna fragments/product/Thermo Fisher
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    otoferlin cdna fragments - by Bioz Stars, 2020-08
    92/100 stars

    Images

    1) Product Images from "A dual‐AAV approach restores fast exocytosis and partially rescues auditory function in deaf otoferlin knock‐out mice"

    Article Title: A dual‐AAV approach restores fast exocytosis and partially rescues auditory function in deaf otoferlin knock‐out mice

    Journal: EMBO Molecular Medicine

    doi: 10.15252/emmm.201809396

    Otoferlin dual‐ AAV ‐ TS ‐transduced Otof −/− organs of Corti express full‐length otoferlin mRNA Schematic representation of otoferlin cDNA from otoferlin dual‐AAV‐transduced, wild‐type, and Otof −/− organs of Corti, displaying binding sites of primers used in PCRs to assess dual‐AAV reassembly. Otoferlin PCR amplicons from organ of Corti cDNA. A 1,753‐bp‐long amplicon (*), also present in non‐injected wild‐type controls (WTB6, WTCD1B6F1), indicates successful reassembly of the split otoferlin expression cassette in otoferlin dual‐AAV‐TS‐transduced CD1B6F1‐ Otof −/− organs of Corti (injected ear). In Otof −/− samples, three shorter products were amplified (a, b and c). Sanger sequencing confirmed correct dual‐AAV split‐site assembly (dashed line) as well as the presence of an artificial AccIII restriction site introduced in the dual‐AAV‐TS otoferlin cDNA, which is absent in the wild‐type (WT) and Otof −/− cDNA (a–c). Amplicons a‐c from Otof −/− organs of Corti all lack exons 14–15, while bands “b” (1,480 bp) and “c” (1,679 bp) still contain intron 20–21 (b) or intron 23–24 (c), respectively. Western blotting on cell lysates of WT and Otof −/− CD1B6F1 organs of Corti. Two bands of ˜210–230 kDa, corresponding to full‐length otoferlin, were detected in WT but absent in Otof −/− ears. (**) refers to an unspecific band detected in both samples. GAPDH was used as loading control. Data information: CDS: coding sequence, Ex: exon, TS: trans‐splicing, Hyb: hybrid, control ear: non‐treated ears, non‐injected ear: contralateral non‐injected Otof −/− ears. Source data are available online for this figure.
    Figure Legend Snippet: Otoferlin dual‐ AAV ‐ TS ‐transduced Otof −/− organs of Corti express full‐length otoferlin mRNA Schematic representation of otoferlin cDNA from otoferlin dual‐AAV‐transduced, wild‐type, and Otof −/− organs of Corti, displaying binding sites of primers used in PCRs to assess dual‐AAV reassembly. Otoferlin PCR amplicons from organ of Corti cDNA. A 1,753‐bp‐long amplicon (*), also present in non‐injected wild‐type controls (WTB6, WTCD1B6F1), indicates successful reassembly of the split otoferlin expression cassette in otoferlin dual‐AAV‐TS‐transduced CD1B6F1‐ Otof −/− organs of Corti (injected ear). In Otof −/− samples, three shorter products were amplified (a, b and c). Sanger sequencing confirmed correct dual‐AAV split‐site assembly (dashed line) as well as the presence of an artificial AccIII restriction site introduced in the dual‐AAV‐TS otoferlin cDNA, which is absent in the wild‐type (WT) and Otof −/− cDNA (a–c). Amplicons a‐c from Otof −/− organs of Corti all lack exons 14–15, while bands “b” (1,480 bp) and “c” (1,679 bp) still contain intron 20–21 (b) or intron 23–24 (c), respectively. Western blotting on cell lysates of WT and Otof −/− CD1B6F1 organs of Corti. Two bands of ˜210–230 kDa, corresponding to full‐length otoferlin, were detected in WT but absent in Otof −/− ears. (**) refers to an unspecific band detected in both samples. GAPDH was used as loading control. Data information: CDS: coding sequence, Ex: exon, TS: trans‐splicing, Hyb: hybrid, control ear: non‐treated ears, non‐injected ear: contralateral non‐injected Otof −/− ears. Source data are available online for this figure.

    Techniques Used: Binding Assay, Polymerase Chain Reaction, Amplification, Injection, Expressing, Sequencing, Western Blot

    Related Articles

    Amplification:

    Article Title: A dual‐AAV approach restores fast exocytosis and partially rescues auditory function in deaf otoferlin knock‐out mice
    Article Snippet: .. Otoferlin cDNA fragments spanning the split‐site of the full‐length otoferlin expression cassette were amplified from the cochlear cDNA using DreamTaq Polymerase (#EP0702, Thermo Fisher Scientific). ..

    Expressing:

    Article Title: A dual‐AAV approach restores fast exocytosis and partially rescues auditory function in deaf otoferlin knock‐out mice
    Article Snippet: .. Otoferlin cDNA fragments spanning the split‐site of the full‐length otoferlin expression cassette were amplified from the cochlear cDNA using DreamTaq Polymerase (#EP0702, Thermo Fisher Scientific). ..

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    Thermo Fisher otoferlin cdna fragments
    <t>Otoferlin</t> dual‐ AAV ‐ TS ‐transduced Otof −/− organs of Corti express full‐length otoferlin mRNA Schematic representation of otoferlin <t>cDNA</t> from otoferlin dual‐AAV‐transduced, wild‐type, and Otof −/− organs of Corti, displaying binding sites of primers used in PCRs to assess dual‐AAV reassembly. Otoferlin PCR amplicons from organ of Corti cDNA. A 1,753‐bp‐long amplicon (*), also present in non‐injected wild‐type controls (WTB6, WTCD1B6F1), indicates successful reassembly of the split otoferlin expression cassette in otoferlin dual‐AAV‐TS‐transduced CD1B6F1‐ Otof −/− organs of Corti (injected ear). In Otof −/− samples, three shorter products were amplified (a, b and c). Sanger sequencing confirmed correct dual‐AAV split‐site assembly (dashed line) as well as the presence of an artificial AccIII restriction site introduced in the dual‐AAV‐TS otoferlin cDNA, which is absent in the wild‐type (WT) and Otof −/− cDNA (a–c). Amplicons a‐c from Otof −/− organs of Corti all lack exons 14–15, while bands “b” (1,480 bp) and “c” (1,679 bp) still contain intron 20–21 (b) or intron 23–24 (c), respectively. Western blotting on cell lysates of WT and Otof −/− CD1B6F1 organs of Corti. Two bands of ˜210–230 kDa, corresponding to full‐length otoferlin, were detected in WT but absent in Otof −/− ears. (**) refers to an unspecific band detected in both samples. GAPDH was used as loading control. Data information: CDS: coding sequence, Ex: exon, TS: trans‐splicing, Hyb: hybrid, control ear: non‐treated ears, non‐injected ear: contralateral non‐injected Otof −/− ears. Source data are available online for this figure.
    Otoferlin Cdna Fragments, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/otoferlin cdna fragments/product/Thermo Fisher
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    otoferlin cdna fragments - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    Image Search Results


    Otoferlin dual‐ AAV ‐ TS ‐transduced Otof −/− organs of Corti express full‐length otoferlin mRNA Schematic representation of otoferlin cDNA from otoferlin dual‐AAV‐transduced, wild‐type, and Otof −/− organs of Corti, displaying binding sites of primers used in PCRs to assess dual‐AAV reassembly. Otoferlin PCR amplicons from organ of Corti cDNA. A 1,753‐bp‐long amplicon (*), also present in non‐injected wild‐type controls (WTB6, WTCD1B6F1), indicates successful reassembly of the split otoferlin expression cassette in otoferlin dual‐AAV‐TS‐transduced CD1B6F1‐ Otof −/− organs of Corti (injected ear). In Otof −/− samples, three shorter products were amplified (a, b and c). Sanger sequencing confirmed correct dual‐AAV split‐site assembly (dashed line) as well as the presence of an artificial AccIII restriction site introduced in the dual‐AAV‐TS otoferlin cDNA, which is absent in the wild‐type (WT) and Otof −/− cDNA (a–c). Amplicons a‐c from Otof −/− organs of Corti all lack exons 14–15, while bands “b” (1,480 bp) and “c” (1,679 bp) still contain intron 20–21 (b) or intron 23–24 (c), respectively. Western blotting on cell lysates of WT and Otof −/− CD1B6F1 organs of Corti. Two bands of ˜210–230 kDa, corresponding to full‐length otoferlin, were detected in WT but absent in Otof −/− ears. (**) refers to an unspecific band detected in both samples. GAPDH was used as loading control. Data information: CDS: coding sequence, Ex: exon, TS: trans‐splicing, Hyb: hybrid, control ear: non‐treated ears, non‐injected ear: contralateral non‐injected Otof −/− ears. Source data are available online for this figure.

    Journal: EMBO Molecular Medicine

    Article Title: A dual‐AAV approach restores fast exocytosis and partially rescues auditory function in deaf otoferlin knock‐out mice

    doi: 10.15252/emmm.201809396

    Figure Lengend Snippet: Otoferlin dual‐ AAV ‐ TS ‐transduced Otof −/− organs of Corti express full‐length otoferlin mRNA Schematic representation of otoferlin cDNA from otoferlin dual‐AAV‐transduced, wild‐type, and Otof −/− organs of Corti, displaying binding sites of primers used in PCRs to assess dual‐AAV reassembly. Otoferlin PCR amplicons from organ of Corti cDNA. A 1,753‐bp‐long amplicon (*), also present in non‐injected wild‐type controls (WTB6, WTCD1B6F1), indicates successful reassembly of the split otoferlin expression cassette in otoferlin dual‐AAV‐TS‐transduced CD1B6F1‐ Otof −/− organs of Corti (injected ear). In Otof −/− samples, three shorter products were amplified (a, b and c). Sanger sequencing confirmed correct dual‐AAV split‐site assembly (dashed line) as well as the presence of an artificial AccIII restriction site introduced in the dual‐AAV‐TS otoferlin cDNA, which is absent in the wild‐type (WT) and Otof −/− cDNA (a–c). Amplicons a‐c from Otof −/− organs of Corti all lack exons 14–15, while bands “b” (1,480 bp) and “c” (1,679 bp) still contain intron 20–21 (b) or intron 23–24 (c), respectively. Western blotting on cell lysates of WT and Otof −/− CD1B6F1 organs of Corti. Two bands of ˜210–230 kDa, corresponding to full‐length otoferlin, were detected in WT but absent in Otof −/− ears. (**) refers to an unspecific band detected in both samples. GAPDH was used as loading control. Data information: CDS: coding sequence, Ex: exon, TS: trans‐splicing, Hyb: hybrid, control ear: non‐treated ears, non‐injected ear: contralateral non‐injected Otof −/− ears. Source data are available online for this figure.

    Article Snippet: Otoferlin cDNA fragments spanning the split‐site of the full‐length otoferlin expression cassette were amplified from the cochlear cDNA using DreamTaq Polymerase (#EP0702, Thermo Fisher Scientific).

    Techniques: Binding Assay, Polymerase Chain Reaction, Amplification, Injection, Expressing, Sequencing, Western Blot