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(a) Representative bright-field images showing morphological changes in BV2 microglia following <t>recombinant</t> <t>OSM</t> stimulation. Compared with untreated cells, OSM-treated BV2 cells exhibited enlarged soma, shortened processes, and a more amoeboid-like morphology, consistent with microglial activation. (b) CCK8 assay detects the cell viability of each group; (c, d) The representative images and quantitative analysis of protein expression of IL-6, TNF-α, and β-actin detected by Western blot; Data were expressed as the mean±SEM, * P < 0.05, ** P < 0.01, vs. indicated groups n = 3 for each group.
Osm Recombinant Protein, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human oncostatin m/osm antibody  (Bio-Techne corporation)
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(a) Representative bright-field images showing morphological changes in BV2 microglia following <t>recombinant</t> <t>OSM</t> stimulation. Compared with untreated cells, OSM-treated BV2 cells exhibited enlarged soma, shortened processes, and a more amoeboid-like morphology, consistent with microglial activation. (b) CCK8 assay detects the cell viability of each group; (c, d) The representative images and quantitative analysis of protein expression of IL-6, TNF-α, and β-actin detected by Western blot; Data were expressed as the mean±SEM, * P < 0.05, ** P < 0.01, vs. indicated groups n = 3 for each group.
Human Oncostatin M/Osm Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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recombinant mouse osm  (Sino Biological)
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( A ) CellChat analysis of TRL subset interactions with AT2s. ( B ) Heatmap showing the interactions of IFNγ and <t>OSM</t> signaling pathways from TRL subsets to AT2s, analyzed by CellChat. ( C ) Plots showing the expression of Osm and Ifng in young and aged TRL subsets. ( D ) Images of organoids derived from AT2s of young mice co-cultured with TRL subsets from young or aged mice. ( E and F ) Quantification of colony-forming efficiency (CFE) and organoid size. ( G ) Images of organoids derived from AT2s of young mice treated with <t>recombinant</t> OSM or IFNγ. ( H and I ) Quantification of colony-forming efficiency (CFE) and organoid size.
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( A ) CellChat analysis of TRL subset interactions with AT2s. ( B ) Heatmap showing the interactions of IFNγ and <t>OSM</t> signaling pathways from TRL subsets to AT2s, analyzed by CellChat. ( C ) Plots showing the expression of Osm and Ifng in young and aged TRL subsets. ( D ) Images of organoids derived from AT2s of young mice co-cultured with TRL subsets from young or aged mice. ( E and F ) Quantification of colony-forming efficiency (CFE) and organoid size. ( G ) Images of organoids derived from AT2s of young mice treated with <t>recombinant</t> OSM or IFNγ. ( H and I ) Quantification of colony-forming efficiency (CFE) and organoid size.
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oncostatin m osm  (Novoprotein)
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( A ) CellChat analysis of TRL subset interactions with AT2s. ( B ) Heatmap showing the interactions of IFNγ and <t>OSM</t> signaling pathways from TRL subsets to AT2s, analyzed by CellChat. ( C ) Plots showing the expression of Osm and Ifng in young and aged TRL subsets. ( D ) Images of organoids derived from AT2s of young mice co-cultured with TRL subsets from young or aged mice. ( E and F ) Quantification of colony-forming efficiency (CFE) and organoid size. ( G ) Images of organoids derived from AT2s of young mice treated with <t>recombinant</t> OSM or IFNγ. ( H and I ) Quantification of colony-forming efficiency (CFE) and organoid size.
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cells  (MedChemExpress)
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( A ) CellChat analysis of TRL subset interactions with AT2s. ( B ) Heatmap showing the interactions of IFNγ and <t>OSM</t> signaling pathways from TRL subsets to AT2s, analyzed by CellChat. ( C ) Plots showing the expression of Osm and Ifng in young and aged TRL subsets. ( D ) Images of organoids derived from AT2s of young mice co-cultured with TRL subsets from young or aged mice. ( E and F ) Quantification of colony-forming efficiency (CFE) and organoid size. ( G ) Images of organoids derived from AT2s of young mice treated with <t>recombinant</t> OSM or IFNγ. ( H and I ) Quantification of colony-forming efficiency (CFE) and organoid size.
Cells, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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osm  (MedChemExpress)
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( A ) CellChat analysis of TRL subset interactions with AT2s. ( B ) Heatmap showing the interactions of IFNγ and <t>OSM</t> signaling pathways from TRL subsets to AT2s, analyzed by CellChat. ( C ) Plots showing the expression of Osm and Ifng in young and aged TRL subsets. ( D ) Images of organoids derived from AT2s of young mice co-cultured with TRL subsets from young or aged mice. ( E and F ) Quantification of colony-forming efficiency (CFE) and organoid size. ( G ) Images of organoids derived from AT2s of young mice treated with <t>recombinant</t> OSM or IFNγ. ( H and I ) Quantification of colony-forming efficiency (CFE) and organoid size.
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( A ) CellChat analysis of TRL subset interactions with AT2s. ( B ) Heatmap showing the interactions of IFNγ and <t>OSM</t> signaling pathways from TRL subsets to AT2s, analyzed by CellChat. ( C ) Plots showing the expression of Osm and Ifng in young and aged TRL subsets. ( D ) Images of organoids derived from AT2s of young mice co-cultured with TRL subsets from young or aged mice. ( E and F ) Quantification of colony-forming efficiency (CFE) and organoid size. ( G ) Images of organoids derived from AT2s of young mice treated with <t>recombinant</t> OSM or IFNγ. ( H and I ) Quantification of colony-forming efficiency (CFE) and organoid size.
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( A ) CellChat analysis of TRL subset interactions with AT2s. ( B ) Heatmap showing the interactions of IFNγ and <t>OSM</t> signaling pathways from TRL subsets to AT2s, analyzed by CellChat. ( C ) Plots showing the expression of Osm and Ifng in young and aged TRL subsets. ( D ) Images of organoids derived from AT2s of young mice co-cultured with TRL subsets from young or aged mice. ( E and F ) Quantification of colony-forming efficiency (CFE) and organoid size. ( G ) Images of organoids derived from AT2s of young mice treated with <t>recombinant</t> OSM or IFNγ. ( H and I ) Quantification of colony-forming efficiency (CFE) and organoid size.
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Image Search Results


(a) Representative bright-field images showing morphological changes in BV2 microglia following recombinant OSM stimulation. Compared with untreated cells, OSM-treated BV2 cells exhibited enlarged soma, shortened processes, and a more amoeboid-like morphology, consistent with microglial activation. (b) CCK8 assay detects the cell viability of each group; (c, d) The representative images and quantitative analysis of protein expression of IL-6, TNF-α, and β-actin detected by Western blot; Data were expressed as the mean±SEM, * P < 0.05, ** P < 0.01, vs. indicated groups n = 3 for each group.

Journal: Frontiers in Neurology

Article Title: Single-cell RNA sequencing reveals microglial proliferative bias and neuroinflammatory communication reprogramming following traumatic brain injury

doi: 10.3389/fneur.2026.1789863

Figure Lengend Snippet: (a) Representative bright-field images showing morphological changes in BV2 microglia following recombinant OSM stimulation. Compared with untreated cells, OSM-treated BV2 cells exhibited enlarged soma, shortened processes, and a more amoeboid-like morphology, consistent with microglial activation. (b) CCK8 assay detects the cell viability of each group; (c, d) The representative images and quantitative analysis of protein expression of IL-6, TNF-α, and β-actin detected by Western blot; Data were expressed as the mean±SEM, * P < 0.05, ** P < 0.01, vs. indicated groups n = 3 for each group.

Article Snippet: When cultures reached 70%−80% confluence, cells were switched to low serum (1% FBS) for 24 h; followed by stimulation with 1, 5, and 10 ug/ml of LPS (Servicebio, GC205009 ) and 25, 50, and 100 ng/ml of OSM recombinant protein (MedChemExpress, HY-P7274) for 24 h, prior to downstream experiments.

Techniques: Recombinant, Activation Assay, CCK-8 Assay, Expressing, Western Blot

( A ) CellChat analysis of TRL subset interactions with AT2s. ( B ) Heatmap showing the interactions of IFNγ and OSM signaling pathways from TRL subsets to AT2s, analyzed by CellChat. ( C ) Plots showing the expression of Osm and Ifng in young and aged TRL subsets. ( D ) Images of organoids derived from AT2s of young mice co-cultured with TRL subsets from young or aged mice. ( E and F ) Quantification of colony-forming efficiency (CFE) and organoid size. ( G ) Images of organoids derived from AT2s of young mice treated with recombinant OSM or IFNγ. ( H and I ) Quantification of colony-forming efficiency (CFE) and organoid size.

Journal: bioRxiv

Article Title: Dysregulated tissue-resident lymphocytes drive senile emphysema by impairing alveolar regeneration

doi: 10.64898/2026.04.22.720146

Figure Lengend Snippet: ( A ) CellChat analysis of TRL subset interactions with AT2s. ( B ) Heatmap showing the interactions of IFNγ and OSM signaling pathways from TRL subsets to AT2s, analyzed by CellChat. ( C ) Plots showing the expression of Osm and Ifng in young and aged TRL subsets. ( D ) Images of organoids derived from AT2s of young mice co-cultured with TRL subsets from young or aged mice. ( E and F ) Quantification of colony-forming efficiency (CFE) and organoid size. ( G ) Images of organoids derived from AT2s of young mice treated with recombinant OSM or IFNγ. ( H and I ) Quantification of colony-forming efficiency (CFE) and organoid size.

Article Snippet: Where applicable, 1 ng/mL recombinant mouse IFNγ (Cat#BE0055-1MG; BioXcell) and 20 ng/mL recombinant mouse OSM (Cat#50112-M08H; SinoBiological) were added to the medium.

Techniques: Protein-Protein interactions, Expressing, Derivative Assay, Cell Culture, Recombinant