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OriginLab originpro 9 0
3D multi-modal imaging and quantification. A. Representative maximum intensity projection of 60 µm deep stack of WKY and SHR ventricles showing from the left: capillaries labelled with BSA-FITC gel, negative image of cells traced with WGA-Alexa Fluor 594, collagen deposition imaged by second-harmonic generation, and merge of the three different channels (vessels in green, cells in red, collagen in blue). Maximum intensity projections were created using ImageJ 2.0.0-rc-71/1.52p ( https://fiji.sc ). B. Heat Map summarizing vascular, cellular and collagen parameters in all the experimental classes. Five different ventricular regions are shown for each experimental class. All the parameters have been normalized on the same scale. RV is right ventricle (above) and LV is left ventricle (below). Heat Maps were created using OriginPro 9.0 ( https://www.originlab.com ).
Originpro 9 0, supplied by OriginLab, used in various techniques. Bioz Stars score: 97/100, based on 384 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/originpro 9 0/product/OriginLab
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Images

1) Product Images from "3D imaging and morphometry of the heart capillary system in spontaneously hypertensive rats and normotensive controls"

Article Title: 3D imaging and morphometry of the heart capillary system in spontaneously hypertensive rats and normotensive controls

Journal: bioRxiv

doi: 10.1101/2020.08.05.237487

3D multi-modal imaging and quantification. A. Representative maximum intensity projection of 60 µm deep stack of WKY and SHR ventricles showing from the left: capillaries labelled with BSA-FITC gel, negative image of cells traced with WGA-Alexa Fluor 594, collagen deposition imaged by second-harmonic generation, and merge of the three different channels (vessels in green, cells in red, collagen in blue). Maximum intensity projections were created using ImageJ 2.0.0-rc-71/1.52p ( https://fiji.sc ). B. Heat Map summarizing vascular, cellular and collagen parameters in all the experimental classes. Five different ventricular regions are shown for each experimental class. All the parameters have been normalized on the same scale. RV is right ventricle (above) and LV is left ventricle (below). Heat Maps were created using OriginPro 9.0 ( https://www.originlab.com ).
Figure Legend Snippet: 3D multi-modal imaging and quantification. A. Representative maximum intensity projection of 60 µm deep stack of WKY and SHR ventricles showing from the left: capillaries labelled with BSA-FITC gel, negative image of cells traced with WGA-Alexa Fluor 594, collagen deposition imaged by second-harmonic generation, and merge of the three different channels (vessels in green, cells in red, collagen in blue). Maximum intensity projections were created using ImageJ 2.0.0-rc-71/1.52p ( https://fiji.sc ). B. Heat Map summarizing vascular, cellular and collagen parameters in all the experimental classes. Five different ventricular regions are shown for each experimental class. All the parameters have been normalized on the same scale. RV is right ventricle (above) and LV is left ventricle (below). Heat Maps were created using OriginPro 9.0 ( https://www.originlab.com ).

Techniques Used: Imaging, Whole Genome Amplification

Vascular disorganization correlate with myocardial disarray. Correlation analysis between vascular morphometries and tissue cytoarchitecture in WKY and SHR ventricles. A. angular dispersion and cellular disarray, B. vasculature density and cellular disarray, C. angular dispersion and collagen percentage, and D vasculature density and collagen content (%). Plots were created using OriginPro 9.0 ( https://www.originlab.com ).
Figure Legend Snippet: Vascular disorganization correlate with myocardial disarray. Correlation analysis between vascular morphometries and tissue cytoarchitecture in WKY and SHR ventricles. A. angular dispersion and cellular disarray, B. vasculature density and cellular disarray, C. angular dispersion and collagen percentage, and D vasculature density and collagen content (%). Plots were created using OriginPro 9.0 ( https://www.originlab.com ).

Techniques Used:

3D imaging of the coronary microcirculation of clarified hearts. A. Diagram showing the main steps of the BSA-FITC/CLARITY protocol. Heart is rapidly isolated, cannulated through the proximal aorta and perfused with fixative solution. Coronaries are stained with BSA-FITC gel and the organ is subjected to modified CLARITY protocol. B. Section of a right ventricle after fixative solution perfusion in PBS (left) and after TDE clearing (right). C. Representative 3D reconstruction of a stack of 450 × 450 × 300 µm 3  acquired with a custom-made Two Photon Fluorescence Microscope. 3D reconstruction was created using ImageJ 2.0.0-rc-71/1.52p ( https://fiji.sc ). D. Validation of the sample preparation protocol: morphometric parameters obtained in acute experiment of ex-vivo hearts (Ex-Vivo) and in BSA-FITC cleared samples (CLARITY). Student T-test applied, p-value = 0.30; 0.31; 0.39. Plots were created using OriginPro 9.0 ( https://www.originlab.com ).
Figure Legend Snippet: 3D imaging of the coronary microcirculation of clarified hearts. A. Diagram showing the main steps of the BSA-FITC/CLARITY protocol. Heart is rapidly isolated, cannulated through the proximal aorta and perfused with fixative solution. Coronaries are stained with BSA-FITC gel and the organ is subjected to modified CLARITY protocol. B. Section of a right ventricle after fixative solution perfusion in PBS (left) and after TDE clearing (right). C. Representative 3D reconstruction of a stack of 450 × 450 × 300 µm 3 acquired with a custom-made Two Photon Fluorescence Microscope. 3D reconstruction was created using ImageJ 2.0.0-rc-71/1.52p ( https://fiji.sc ). D. Validation of the sample preparation protocol: morphometric parameters obtained in acute experiment of ex-vivo hearts (Ex-Vivo) and in BSA-FITC cleared samples (CLARITY). Student T-test applied, p-value = 0.30; 0.31; 0.39. Plots were created using OriginPro 9.0 ( https://www.originlab.com ).

Techniques Used: Imaging, Isolation, Staining, Modification, Fluorescence, Microscopy, Sample Prep, Ex Vivo

2) Product Images from "Decursin Alleviates Mechanical Allodynia in a Paclitaxel-Induced Neuropathic Pain Mouse Model"

Article Title: Decursin Alleviates Mechanical Allodynia in a Paclitaxel-Induced Neuropathic Pain Mouse Model

Journal: Cells

doi: 10.3390/cells10030547

Intracellular Ca 2+ levels after treatment with decursin in TRPV1-expressing HEK293 cells sensitized by capsaicin. These cells were exposed to different concentrations of decursin in the presence of capsaicin. The Δ340/380 ratio (%) at each decursin concentration was calculated by the same method as that reported in Figure 1 B. The red line indicates the dose–response curve that was obtained using OriginPro software. This experiment was conducted in triplicate.
Figure Legend Snippet: Intracellular Ca 2+ levels after treatment with decursin in TRPV1-expressing HEK293 cells sensitized by capsaicin. These cells were exposed to different concentrations of decursin in the presence of capsaicin. The Δ340/380 ratio (%) at each decursin concentration was calculated by the same method as that reported in Figure 1 B. The red line indicates the dose–response curve that was obtained using OriginPro software. This experiment was conducted in triplicate.

Techniques Used: Expressing, Concentration Assay, Software

3) Product Images from "3D imaging and morphometry of the heart capillary system in spontaneously hypertensive rats and normotensive controls"

Article Title: 3D imaging and morphometry of the heart capillary system in spontaneously hypertensive rats and normotensive controls

Journal: Scientific Reports

doi: 10.1038/s41598-020-71174-9

3D multicolor imaging and quantification. ( A ) Representative maximum intensity projection of 60 µm deep stack of WKY and SHR ventricles showing from the left: capillaries labelled with BSA-FITC gel, negative image of cells traced with WGA-Alexa Fluor 594, collagen deposition imaged by second-harmonic generation, and merge of the three different channels (vessels in green, cells in red, collagen in blue). Maximum intensity projections were created using ImageJ 2.0.0-rc-71/1.52p ( https://fiji.sc ). ( B ) Heat Map summarizing vascular, cellular and collagen parameters in all the experimental classes. Five different ventricular regions are shown for each experimental class. All the parameters have been normalized on the same scale. RV is right ventricle (above) and LV is left ventricle (below). Heat Maps were created using OriginPro 9.0 ( https://www.originlab.com ).
Figure Legend Snippet: 3D multicolor imaging and quantification. ( A ) Representative maximum intensity projection of 60 µm deep stack of WKY and SHR ventricles showing from the left: capillaries labelled with BSA-FITC gel, negative image of cells traced with WGA-Alexa Fluor 594, collagen deposition imaged by second-harmonic generation, and merge of the three different channels (vessels in green, cells in red, collagen in blue). Maximum intensity projections were created using ImageJ 2.0.0-rc-71/1.52p ( https://fiji.sc ). ( B ) Heat Map summarizing vascular, cellular and collagen parameters in all the experimental classes. Five different ventricular regions are shown for each experimental class. All the parameters have been normalized on the same scale. RV is right ventricle (above) and LV is left ventricle (below). Heat Maps were created using OriginPro 9.0 ( https://www.originlab.com ).

Techniques Used: Imaging, Whole Genome Amplification

Vascular disorganization correlate with myocardial disarray. Correlation analysis between vascular morphometries and tissue cytoarchitecture in WKY and SHR ventricles. ( A ) angular dispersion and cellular disarray, ( B ) vasculature density and cellular disarray, ( C ) angular dispersion and collagen percentage, and ( D ) vasculature density and collagen content (%). Plots were created using OriginPro 9.0 ( https://www.originlab.com ).
Figure Legend Snippet: Vascular disorganization correlate with myocardial disarray. Correlation analysis between vascular morphometries and tissue cytoarchitecture in WKY and SHR ventricles. ( A ) angular dispersion and cellular disarray, ( B ) vasculature density and cellular disarray, ( C ) angular dispersion and collagen percentage, and ( D ) vasculature density and collagen content (%). Plots were created using OriginPro 9.0 ( https://www.originlab.com ).

Techniques Used:

3D imaging of the coronary microcirculation of clarified hearts. ( A ) Diagram showing the main steps of the BSA-FITC/CLARITY protocol. Heart is rapidly isolated, cannulated through the proximal aorta and perfused with fixative solution. Coronaries are stained with BSA-FITC gel and the organ is subjected to modified CLARITY protocol. ( B ) Section of a right ventricle after fixative solution perfusion in PBS (left) and after TDE clearing (right). ( C ) Representative 3D reconstruction of a stack of 450 × 450 × 300 µm 3  acquired with a custom-made Two Photon Fluorescence Microscope. 3D reconstruction was created using ImageJ 2.0.0-rc-71/1.52p ( https://fiji.sc ). ( D ) Validation of the sample preparation protocol: morphometric parameters obtained in acute experiment of ex-vivo hearts (Ex-Vivo) and in BSA-FITC cleared samples (CLARITY). Student  t  test applied,  p  value = 0.30; 0.31; 0.39. Plots were created using OriginPro 9.0 ( https://www.originlab.com ).
Figure Legend Snippet: 3D imaging of the coronary microcirculation of clarified hearts. ( A ) Diagram showing the main steps of the BSA-FITC/CLARITY protocol. Heart is rapidly isolated, cannulated through the proximal aorta and perfused with fixative solution. Coronaries are stained with BSA-FITC gel and the organ is subjected to modified CLARITY protocol. ( B ) Section of a right ventricle after fixative solution perfusion in PBS (left) and after TDE clearing (right). ( C ) Representative 3D reconstruction of a stack of 450 × 450 × 300 µm 3 acquired with a custom-made Two Photon Fluorescence Microscope. 3D reconstruction was created using ImageJ 2.0.0-rc-71/1.52p ( https://fiji.sc ). ( D ) Validation of the sample preparation protocol: morphometric parameters obtained in acute experiment of ex-vivo hearts (Ex-Vivo) and in BSA-FITC cleared samples (CLARITY). Student t test applied, p value = 0.30; 0.31; 0.39. Plots were created using OriginPro 9.0 ( https://www.originlab.com ).

Techniques Used: Imaging, Isolation, Staining, Modification, Fluorescence, Microscopy, Sample Prep, Ex Vivo

4) Product Images from "Autofluorescence enhancement for label-free imaging of myelinated fibers in mammalian brains"

Article Title: Autofluorescence enhancement for label-free imaging of myelinated fibers in mammalian brains

Journal: Scientific Reports

doi: 10.1038/s41598-021-86092-7

The MAGIC protocol. ( a ) Representative TPFM images of a mouse brain section during the three subsequent steps of the MAGIC protocol: fixation (PFA), glycerolization (Gly), and washing (MAGIC). The images show different areas of the caudate putamen. For each condition, a fiber bundle is indicated with a red arrow. Scale bar = 50 μm. ( b ) 3D reconstruction of myelinated fibers, imaging performed with TPFM at the resolution of (0.44 × 0.44 × 1) μm 3 . Box scale = 10 μm. ( c ) Measurement (mean ± std.err) of photons emitted by the myelinated fibers (fiber) and the surrounding tissue (tissue) during the different steps of the protocol (PFA, Gly, MAGIC) detected at different excitation wavelengths. ( d ) Intensity contrast (mean ± std.err) observed at different excitation wavelengths during the three steps of the MAGIC protocol. (e) Images of a mouse brain section treated with MAGIC and labeled with FluoroMyelin red. In green and red respectively, the autofluorescence and the exogenous signals are shown. Images were obtained with TPFM, scale bar = 50 μm. Images and the 3D rendering were prepared using Fiji ( www.fiji.sc/Fiji )   20 , graphs were prepared using OriginPro 9.0 ( www.originlab.com ).
Figure Legend Snippet: The MAGIC protocol. ( a ) Representative TPFM images of a mouse brain section during the three subsequent steps of the MAGIC protocol: fixation (PFA), glycerolization (Gly), and washing (MAGIC). The images show different areas of the caudate putamen. For each condition, a fiber bundle is indicated with a red arrow. Scale bar = 50 μm. ( b ) 3D reconstruction of myelinated fibers, imaging performed with TPFM at the resolution of (0.44 × 0.44 × 1) μm 3 . Box scale = 10 μm. ( c ) Measurement (mean ± std.err) of photons emitted by the myelinated fibers (fiber) and the surrounding tissue (tissue) during the different steps of the protocol (PFA, Gly, MAGIC) detected at different excitation wavelengths. ( d ) Intensity contrast (mean ± std.err) observed at different excitation wavelengths during the three steps of the MAGIC protocol. (e) Images of a mouse brain section treated with MAGIC and labeled with FluoroMyelin red. In green and red respectively, the autofluorescence and the exogenous signals are shown. Images were obtained with TPFM, scale bar = 50 μm. Images and the 3D rendering were prepared using Fiji ( www.fiji.sc/Fiji ) 20 , graphs were prepared using OriginPro 9.0 ( www.originlab.com ).

Techniques Used: Imaging, Labeling

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    OriginLab originpro 9 0
    3D multi-modal imaging and quantification. A. Representative maximum intensity projection of 60 µm deep stack of WKY and SHR ventricles showing from the left: capillaries labelled with BSA-FITC gel, negative image of cells traced with WGA-Alexa Fluor 594, collagen deposition imaged by second-harmonic generation, and merge of the three different channels (vessels in green, cells in red, collagen in blue). Maximum intensity projections were created using ImageJ 2.0.0-rc-71/1.52p ( https://fiji.sc ). B. Heat Map summarizing vascular, cellular and collagen parameters in all the experimental classes. Five different ventricular regions are shown for each experimental class. All the parameters have been normalized on the same scale. RV is right ventricle (above) and LV is left ventricle (below). Heat Maps were created using OriginPro 9.0 ( https://www.originlab.com ).
    Originpro 9 0, supplied by OriginLab, used in various techniques. Bioz Stars score: 98/100, based on 318 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/originpro 9 0/product/OriginLab
    Average 98 stars, based on 318 article reviews
    Price from $9.99 to $1999.99
    originpro 9 0 - by Bioz Stars, 2022-09
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    3D multi-modal imaging and quantification. A. Representative maximum intensity projection of 60 µm deep stack of WKY and SHR ventricles showing from the left: capillaries labelled with BSA-FITC gel, negative image of cells traced with WGA-Alexa Fluor 594, collagen deposition imaged by second-harmonic generation, and merge of the three different channels (vessels in green, cells in red, collagen in blue). Maximum intensity projections were created using ImageJ 2.0.0-rc-71/1.52p ( https://fiji.sc ). B. Heat Map summarizing vascular, cellular and collagen parameters in all the experimental classes. Five different ventricular regions are shown for each experimental class. All the parameters have been normalized on the same scale. RV is right ventricle (above) and LV is left ventricle (below). Heat Maps were created using OriginPro 9.0 ( https://www.originlab.com ).

    Journal: bioRxiv

    Article Title: 3D imaging and morphometry of the heart capillary system in spontaneously hypertensive rats and normotensive controls

    doi: 10.1101/2020.08.05.237487

    Figure Lengend Snippet: 3D multi-modal imaging and quantification. A. Representative maximum intensity projection of 60 µm deep stack of WKY and SHR ventricles showing from the left: capillaries labelled with BSA-FITC gel, negative image of cells traced with WGA-Alexa Fluor 594, collagen deposition imaged by second-harmonic generation, and merge of the three different channels (vessels in green, cells in red, collagen in blue). Maximum intensity projections were created using ImageJ 2.0.0-rc-71/1.52p ( https://fiji.sc ). B. Heat Map summarizing vascular, cellular and collagen parameters in all the experimental classes. Five different ventricular regions are shown for each experimental class. All the parameters have been normalized on the same scale. RV is right ventricle (above) and LV is left ventricle (below). Heat Maps were created using OriginPro 9.0 ( https://www.originlab.com ).

    Article Snippet: Data analysisGraphs and data statistical analyses were achieved using OriginPro 9.0 (OriginLab Corporation).

    Techniques: Imaging, Whole Genome Amplification

    Vascular disorganization correlate with myocardial disarray. Correlation analysis between vascular morphometries and tissue cytoarchitecture in WKY and SHR ventricles. A. angular dispersion and cellular disarray, B. vasculature density and cellular disarray, C. angular dispersion and collagen percentage, and D vasculature density and collagen content (%). Plots were created using OriginPro 9.0 ( https://www.originlab.com ).

    Journal: bioRxiv

    Article Title: 3D imaging and morphometry of the heart capillary system in spontaneously hypertensive rats and normotensive controls

    doi: 10.1101/2020.08.05.237487

    Figure Lengend Snippet: Vascular disorganization correlate with myocardial disarray. Correlation analysis between vascular morphometries and tissue cytoarchitecture in WKY and SHR ventricles. A. angular dispersion and cellular disarray, B. vasculature density and cellular disarray, C. angular dispersion and collagen percentage, and D vasculature density and collagen content (%). Plots were created using OriginPro 9.0 ( https://www.originlab.com ).

    Article Snippet: Data analysisGraphs and data statistical analyses were achieved using OriginPro 9.0 (OriginLab Corporation).

    Techniques:

    3D imaging of the coronary microcirculation of clarified hearts. A. Diagram showing the main steps of the BSA-FITC/CLARITY protocol. Heart is rapidly isolated, cannulated through the proximal aorta and perfused with fixative solution. Coronaries are stained with BSA-FITC gel and the organ is subjected to modified CLARITY protocol. B. Section of a right ventricle after fixative solution perfusion in PBS (left) and after TDE clearing (right). C. Representative 3D reconstruction of a stack of 450 × 450 × 300 µm 3  acquired with a custom-made Two Photon Fluorescence Microscope. 3D reconstruction was created using ImageJ 2.0.0-rc-71/1.52p ( https://fiji.sc ). D. Validation of the sample preparation protocol: morphometric parameters obtained in acute experiment of ex-vivo hearts (Ex-Vivo) and in BSA-FITC cleared samples (CLARITY). Student T-test applied, p-value = 0.30; 0.31; 0.39. Plots were created using OriginPro 9.0 ( https://www.originlab.com ).

    Journal: bioRxiv

    Article Title: 3D imaging and morphometry of the heart capillary system in spontaneously hypertensive rats and normotensive controls

    doi: 10.1101/2020.08.05.237487

    Figure Lengend Snippet: 3D imaging of the coronary microcirculation of clarified hearts. A. Diagram showing the main steps of the BSA-FITC/CLARITY protocol. Heart is rapidly isolated, cannulated through the proximal aorta and perfused with fixative solution. Coronaries are stained with BSA-FITC gel and the organ is subjected to modified CLARITY protocol. B. Section of a right ventricle after fixative solution perfusion in PBS (left) and after TDE clearing (right). C. Representative 3D reconstruction of a stack of 450 × 450 × 300 µm 3 acquired with a custom-made Two Photon Fluorescence Microscope. 3D reconstruction was created using ImageJ 2.0.0-rc-71/1.52p ( https://fiji.sc ). D. Validation of the sample preparation protocol: morphometric parameters obtained in acute experiment of ex-vivo hearts (Ex-Vivo) and in BSA-FITC cleared samples (CLARITY). Student T-test applied, p-value = 0.30; 0.31; 0.39. Plots were created using OriginPro 9.0 ( https://www.originlab.com ).

    Article Snippet: Data analysisGraphs and data statistical analyses were achieved using OriginPro 9.0 (OriginLab Corporation).

    Techniques: Imaging, Isolation, Staining, Modification, Fluorescence, Microscopy, Sample Prep, Ex Vivo

    Intracellular Ca 2+ levels after treatment with decursin in TRPV1-expressing HEK293 cells sensitized by capsaicin. These cells were exposed to different concentrations of decursin in the presence of capsaicin. The Δ340/380 ratio (%) at each decursin concentration was calculated by the same method as that reported in Figure 1 B. The red line indicates the dose–response curve that was obtained using OriginPro software. This experiment was conducted in triplicate.

    Journal: Cells

    Article Title: Decursin Alleviates Mechanical Allodynia in a Paclitaxel-Induced Neuropathic Pain Mouse Model

    doi: 10.3390/cells10030547

    Figure Lengend Snippet: Intracellular Ca 2+ levels after treatment with decursin in TRPV1-expressing HEK293 cells sensitized by capsaicin. These cells were exposed to different concentrations of decursin in the presence of capsaicin. The Δ340/380 ratio (%) at each decursin concentration was calculated by the same method as that reported in Figure 1 B. The red line indicates the dose–response curve that was obtained using OriginPro software. This experiment was conducted in triplicate.

    Article Snippet: The half maximal inhibitory concentration (IC50) of decursin was calculated after dose–response fitting using OriginPro software (9.0, OriginLab, Northampton, MA, USA).

    Techniques: Expressing, Concentration Assay, Software

    3D multicolor imaging and quantification. ( A ) Representative maximum intensity projection of 60 µm deep stack of WKY and SHR ventricles showing from the left: capillaries labelled with BSA-FITC gel, negative image of cells traced with WGA-Alexa Fluor 594, collagen deposition imaged by second-harmonic generation, and merge of the three different channels (vessels in green, cells in red, collagen in blue). Maximum intensity projections were created using ImageJ 2.0.0-rc-71/1.52p ( https://fiji.sc ). ( B ) Heat Map summarizing vascular, cellular and collagen parameters in all the experimental classes. Five different ventricular regions are shown for each experimental class. All the parameters have been normalized on the same scale. RV is right ventricle (above) and LV is left ventricle (below). Heat Maps were created using OriginPro 9.0 ( https://www.originlab.com ).

    Journal: Scientific Reports

    Article Title: 3D imaging and morphometry of the heart capillary system in spontaneously hypertensive rats and normotensive controls

    doi: 10.1038/s41598-020-71174-9

    Figure Lengend Snippet: 3D multicolor imaging and quantification. ( A ) Representative maximum intensity projection of 60 µm deep stack of WKY and SHR ventricles showing from the left: capillaries labelled with BSA-FITC gel, negative image of cells traced with WGA-Alexa Fluor 594, collagen deposition imaged by second-harmonic generation, and merge of the three different channels (vessels in green, cells in red, collagen in blue). Maximum intensity projections were created using ImageJ 2.0.0-rc-71/1.52p ( https://fiji.sc ). ( B ) Heat Map summarizing vascular, cellular and collagen parameters in all the experimental classes. Five different ventricular regions are shown for each experimental class. All the parameters have been normalized on the same scale. RV is right ventricle (above) and LV is left ventricle (below). Heat Maps were created using OriginPro 9.0 ( https://www.originlab.com ).

    Article Snippet: Data analysisGraphs and data statistical analyses were achieved using OriginPro 9.0 (OriginLab Corporation).

    Techniques: Imaging, Whole Genome Amplification

    Vascular disorganization correlate with myocardial disarray. Correlation analysis between vascular morphometries and tissue cytoarchitecture in WKY and SHR ventricles. ( A ) angular dispersion and cellular disarray, ( B ) vasculature density and cellular disarray, ( C ) angular dispersion and collagen percentage, and ( D ) vasculature density and collagen content (%). Plots were created using OriginPro 9.0 ( https://www.originlab.com ).

    Journal: Scientific Reports

    Article Title: 3D imaging and morphometry of the heart capillary system in spontaneously hypertensive rats and normotensive controls

    doi: 10.1038/s41598-020-71174-9

    Figure Lengend Snippet: Vascular disorganization correlate with myocardial disarray. Correlation analysis between vascular morphometries and tissue cytoarchitecture in WKY and SHR ventricles. ( A ) angular dispersion and cellular disarray, ( B ) vasculature density and cellular disarray, ( C ) angular dispersion and collagen percentage, and ( D ) vasculature density and collagen content (%). Plots were created using OriginPro 9.0 ( https://www.originlab.com ).

    Article Snippet: Data analysisGraphs and data statistical analyses were achieved using OriginPro 9.0 (OriginLab Corporation).

    Techniques:

    3D imaging of the coronary microcirculation of clarified hearts. ( A ) Diagram showing the main steps of the BSA-FITC/CLARITY protocol. Heart is rapidly isolated, cannulated through the proximal aorta and perfused with fixative solution. Coronaries are stained with BSA-FITC gel and the organ is subjected to modified CLARITY protocol. ( B ) Section of a right ventricle after fixative solution perfusion in PBS (left) and after TDE clearing (right). ( C ) Representative 3D reconstruction of a stack of 450 × 450 × 300 µm 3  acquired with a custom-made Two Photon Fluorescence Microscope. 3D reconstruction was created using ImageJ 2.0.0-rc-71/1.52p ( https://fiji.sc ). ( D ) Validation of the sample preparation protocol: morphometric parameters obtained in acute experiment of ex-vivo hearts (Ex-Vivo) and in BSA-FITC cleared samples (CLARITY). Student  t  test applied,  p  value = 0.30; 0.31; 0.39. Plots were created using OriginPro 9.0 ( https://www.originlab.com ).

    Journal: Scientific Reports

    Article Title: 3D imaging and morphometry of the heart capillary system in spontaneously hypertensive rats and normotensive controls

    doi: 10.1038/s41598-020-71174-9

    Figure Lengend Snippet: 3D imaging of the coronary microcirculation of clarified hearts. ( A ) Diagram showing the main steps of the BSA-FITC/CLARITY protocol. Heart is rapidly isolated, cannulated through the proximal aorta and perfused with fixative solution. Coronaries are stained with BSA-FITC gel and the organ is subjected to modified CLARITY protocol. ( B ) Section of a right ventricle after fixative solution perfusion in PBS (left) and after TDE clearing (right). ( C ) Representative 3D reconstruction of a stack of 450 × 450 × 300 µm 3 acquired with a custom-made Two Photon Fluorescence Microscope. 3D reconstruction was created using ImageJ 2.0.0-rc-71/1.52p ( https://fiji.sc ). ( D ) Validation of the sample preparation protocol: morphometric parameters obtained in acute experiment of ex-vivo hearts (Ex-Vivo) and in BSA-FITC cleared samples (CLARITY). Student t test applied, p value = 0.30; 0.31; 0.39. Plots were created using OriginPro 9.0 ( https://www.originlab.com ).

    Article Snippet: Data analysisGraphs and data statistical analyses were achieved using OriginPro 9.0 (OriginLab Corporation).

    Techniques: Imaging, Isolation, Staining, Modification, Fluorescence, Microscopy, Sample Prep, Ex Vivo

    The MAGIC protocol. ( a ) Representative TPFM images of a mouse brain section during the three subsequent steps of the MAGIC protocol: fixation (PFA), glycerolization (Gly), and washing (MAGIC). The images show different areas of the caudate putamen. For each condition, a fiber bundle is indicated with a red arrow. Scale bar = 50 μm. ( b ) 3D reconstruction of myelinated fibers, imaging performed with TPFM at the resolution of (0.44 × 0.44 × 1) μm 3 . Box scale = 10 μm. ( c ) Measurement (mean ± std.err) of photons emitted by the myelinated fibers (fiber) and the surrounding tissue (tissue) during the different steps of the protocol (PFA, Gly, MAGIC) detected at different excitation wavelengths. ( d ) Intensity contrast (mean ± std.err) observed at different excitation wavelengths during the three steps of the MAGIC protocol. (e) Images of a mouse brain section treated with MAGIC and labeled with FluoroMyelin red. In green and red respectively, the autofluorescence and the exogenous signals are shown. Images were obtained with TPFM, scale bar = 50 μm. Images and the 3D rendering were prepared using Fiji ( www.fiji.sc/Fiji )   20 , graphs were prepared using OriginPro 9.0 ( www.originlab.com ).

    Journal: Scientific Reports

    Article Title: Autofluorescence enhancement for label-free imaging of myelinated fibers in mammalian brains

    doi: 10.1038/s41598-021-86092-7

    Figure Lengend Snippet: The MAGIC protocol. ( a ) Representative TPFM images of a mouse brain section during the three subsequent steps of the MAGIC protocol: fixation (PFA), glycerolization (Gly), and washing (MAGIC). The images show different areas of the caudate putamen. For each condition, a fiber bundle is indicated with a red arrow. Scale bar = 50 μm. ( b ) 3D reconstruction of myelinated fibers, imaging performed with TPFM at the resolution of (0.44 × 0.44 × 1) μm 3 . Box scale = 10 μm. ( c ) Measurement (mean ± std.err) of photons emitted by the myelinated fibers (fiber) and the surrounding tissue (tissue) during the different steps of the protocol (PFA, Gly, MAGIC) detected at different excitation wavelengths. ( d ) Intensity contrast (mean ± std.err) observed at different excitation wavelengths during the three steps of the MAGIC protocol. (e) Images of a mouse brain section treated with MAGIC and labeled with FluoroMyelin red. In green and red respectively, the autofluorescence and the exogenous signals are shown. Images were obtained with TPFM, scale bar = 50 μm. Images and the 3D rendering were prepared using Fiji ( www.fiji.sc/Fiji ) 20 , graphs were prepared using OriginPro 9.0 ( www.originlab.com ).

    Article Snippet: Data analysis Graphs and statistical analyses were done with OriginPro 9.0 (OriginLab Corporation: www.originlab.com ).

    Techniques: Imaging, Labeling