orbitrap q exactive hf spectrometer  (Thermo Fisher)


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    Q Exactive HF Hybrid Quadrupole Orbitrap Mass Spectrometer
    Description:
    Identify and quantify more proteins peptides lipids glycans and small molecules accurately and in less time with the Thermo Scientific Q Exactive HF hybrid quadrupole Orbitrap mass spectrometer The Q Exactive HF system combines a state of the art segmented quadrupole for high performance precursor ion selection with a high resolution accurate mass HR AM ultra high field Orbitrap mass analyzer to deliver a superior combination of scan speed resolving power mass accuracy spectral quality and sensitivity Identify quantify and confirm in a single analysis with a single instrument with the Q Exactive HF mass spectrometer
    Catalog Number:
    iqlaaegaapfalgmbfz
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    Applications:
    Industrial & Applied Science|Industrial Mass Spectrometry
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    Instruments and Equipment
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    Structured Review

    Thermo Fisher orbitrap q exactive hf spectrometer
    Identify and quantify more proteins peptides lipids glycans and small molecules accurately and in less time with the Thermo Scientific Q Exactive HF hybrid quadrupole Orbitrap mass spectrometer The Q Exactive HF system combines a state of the art segmented quadrupole for high performance precursor ion selection with a high resolution accurate mass HR AM ultra high field Orbitrap mass analyzer to deliver a superior combination of scan speed resolving power mass accuracy spectral quality and sensitivity Identify quantify and confirm in a single analysis with a single instrument with the Q Exactive HF mass spectrometer
    https://www.bioz.com/result/orbitrap q exactive hf spectrometer/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    orbitrap q exactive hf spectrometer - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    High Performance Liquid Chromatography:

    Article Title: In Vitro Toxicity Assessment of Emitted Materials Collected during the Manufacture of Water Pipe Plastic Linings
    Article Snippet: .. 1 μg of peptides from each sample were analyzed by reverse-phase HPLC-ESI-MS using a Dionex UltiMate 3000 RSLC Nano System (Thermo Scientific) which was directly connected to a Q-Exactive HF Hybrid Quadrupole-Orbitrap MS (Thermo Scientific) and a Nanospray Flex Ion Source (Thermo Scientific). .. Peptides were loaded onto a trap column (300 μm ID × 5 mm, 5 μm 100 Å PepMap C18 medium) at a 5 μL/min flowrate using 98% purified water/2% ACN/0.01% FA solvent system.

    Mass Spectrometry:

    Article Title: Noncovalently Associated Peptides Observed during Liquid Chromatography-Mass Spectrometry and Their Effect on Cross-Link Analyses
    Article Snippet: .. The eluting peptides were sprayed into a Q Exactive High-field (HF) Hybrid Quadrupole-Orbitrap Mass Spectrometer (Thermo Fisher Scientific, Bremen, Germany). ..

    Article Title: A robust and versatile nanobody platform for drug delivery
    Article Snippet: .. After proteolysis, the peptide mixtures were desalted and analyzed with a nano-LC 1200 (Thermo Fisher) coupled to a Q Exactive™ HF-X Hybrid Quadrupole-Orbitrap™ mass spectrometer (Thermo Fisher). .. The cross-linked peptides were loaded onto a picochip column (C18, 3□μm particle size, 300□Å pore size, 50□μm□×□10.5□cm; New Objective) and eluted using a 60□min LC gradient : 5% B–8% B, 0 – 5 min; 8% B – 32% B, 5 – 45 min; 32% B–100% B, 45 – 49 min; 100% B, 49 −54 min; 100% B - 5 % B, 54 min - 54 min 10 sec; 5% B, 54 min 10 sec - 60 min 10 sec; mobile phase A consisted of 0.1% formic acid (FA), and mobile phase B consisted of 0.1% FA in 80% acetonitrile.

    Article Title: (Phospho)proteomic Profiling of Microsatellite Unstable CRC Cells Reveals Alterations in Nuclear Signaling and Cholesterol Metabolism Caused by Frameshift Mutation of NMD Regulator UPF3A
    Article Snippet: .. The nanoUPLC system was coupled online to a Q Exactive HF-X Hybrid Quadrupole-Orbitrap mass spectrometer (Thermo Fisher Scientific). .. The following parameters were set: ESI voltage 2200 V; capillary temperature 275 °C, normalized collision energy 35 V. Data were acquired by scan cycles of one FTMS (Fourier-transform mass spectrometry) scan with a resolution of 120,000 at m/z 200 and a range from 300 to 2000 m/z in parallel with ten MS/MS scans in the ion trap of the most abundant precursor ions.

    Article Title: eIF4A2 drives repression of translation at initiation by Ccr4-Not through purine-rich motifs in the 5′UTR
    Article Snippet: .. Peptide samples were run on the Q-Exactive HF mass spectrometer coupled to an EASY-nLC II 1200 chromatography system (Thermo Scientific). ..

    Article Title: Serial-omics of P53−/−, Brca1−/− Mouse Breast Tumor and Normal Mammary Gland
    Article Snippet: .. The other half of the metabolite samples were re-suspended in 30 μL LC/MS grade water, 5 μL were analyzed by positive negative switching mode using a high resolution QExactive HF hybrid quadrupole-Orbitrap mass spectrometer (Thermo Fisher Scientific) via a Top 8 DDA. .. Metabolites were delivered and separated using an EASY-nLC nanoflow HPLC (Thermo Fisher Scientific) at 225 nL/min using self-packed 15 cm length × 75 μm i.d.

    Article Title: Novel functions of the ubiquitin-independent proteasome system in regulating Xenopus germline development
    Article Snippet: .. An UltimateTM 3000 RSLCnano System nano liquid chromatography system was coupled to a Q-Exactive HF-X Hybrid Quadrupole-Orbitrap Mass spectrometer (Thermo Scientific) for proteomic data acquisition. .. Proteomic data analysis was performed using Spectronaut 10 (Biognosys) software.

    Article Title: Dietary sphinganine is selectively assimilated by members of the gut microbiome
    Article Snippet: .. Mass Spectrometric Analysis High resolution LC-MS (LC-HRMS) analysis was performed on a Thermo Fisher Scientific Vanquish Horizon UHPLC System coupled with a Thermo Q Exactive HF hybrid quadrupole-orbitrap high-resolution mass spectrometer equipped with a HESI ion source. .. 3 μL of extract was injected and separated using a water-acetonitrile gradient on a Kinetex EVO C18 column (150 mm × 2.1 mm, particle size 1.7 μm, part number: 00F-4726-AN) maintained at 40°C.

    Liquid Chromatography:

    Article Title: Novel functions of the ubiquitin-independent proteasome system in regulating Xenopus germline development
    Article Snippet: .. An UltimateTM 3000 RSLCnano System nano liquid chromatography system was coupled to a Q-Exactive HF-X Hybrid Quadrupole-Orbitrap Mass spectrometer (Thermo Scientific) for proteomic data acquisition. .. Proteomic data analysis was performed using Spectronaut 10 (Biognosys) software.

    Chromatography:

    Article Title: eIF4A2 drives repression of translation at initiation by Ccr4-Not through purine-rich motifs in the 5′UTR
    Article Snippet: .. Peptide samples were run on the Q-Exactive HF mass spectrometer coupled to an EASY-nLC II 1200 chromatography system (Thermo Scientific). ..

    Liquid Chromatography with Mass Spectroscopy:

    Article Title: Serial-omics of P53−/−, Brca1−/− Mouse Breast Tumor and Normal Mammary Gland
    Article Snippet: .. The other half of the metabolite samples were re-suspended in 30 μL LC/MS grade water, 5 μL were analyzed by positive negative switching mode using a high resolution QExactive HF hybrid quadrupole-Orbitrap mass spectrometer (Thermo Fisher Scientific) via a Top 8 DDA. .. Metabolites were delivered and separated using an EASY-nLC nanoflow HPLC (Thermo Fisher Scientific) at 225 nL/min using self-packed 15 cm length × 75 μm i.d.

    Article Title: Dietary sphinganine is selectively assimilated by members of the gut microbiome
    Article Snippet: .. Mass Spectrometric Analysis High resolution LC-MS (LC-HRMS) analysis was performed on a Thermo Fisher Scientific Vanquish Horizon UHPLC System coupled with a Thermo Q Exactive HF hybrid quadrupole-orbitrap high-resolution mass spectrometer equipped with a HESI ion source. .. 3 μL of extract was injected and separated using a water-acetonitrile gradient on a Kinetex EVO C18 column (150 mm × 2.1 mm, particle size 1.7 μm, part number: 00F-4726-AN) maintained at 40°C.

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  • 99
    Thermo Fisher quadrupole orbitrap mass spectrometers
    Deep single-run proteomics of cell lines and human ovarian cancer tissue. ( a ) Summary of the shotgun proteomics workflow for OvCa cellular models and high-grade serous ovarian cancer (HGSOC) tissues. Following lysis, protein purification, and tryptic digest, peptides were separated by ultra-high performance liquid chromatography and measured in single runs using a quadrupole <t>Orbitrap</t> mass spectrometer. Label-free proteome quantification was performed using the MaxQuant software environment. ( b ) Workflow reproducibility for cell lines ( n =30), HGSOC tissues ( n =8) and primary FTEC cells ( n =3). Pearson correlations ( r ) were calculated for biological replicates of cell lines, primary FTEC isolates from different healthy donors, and ovarian tumour tissues from both ovaries from a woman with HGSOC. ( c ) Average number of quantified proteins from each sample type. Error bars represent standard deviations. ( d ) Number of proteins common to FTECs, HGSOC tumours and cell lines. FTEC, fallopian tube epithelial cell; LC-MS/MS, liquid chromatography tandem mass spectrometry; m/z , mass-to-charge ratio; uHPLC, ultra-high performance liquid chromatography.
    Quadrupole Orbitrap Mass Spectrometers, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quadrupole orbitrap mass spectrometers/product/Thermo Fisher
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    quadrupole orbitrap mass spectrometers - by Bioz Stars, 2020-09
    99/100 stars
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    99
    Thermo Fisher high resolution mass spectrometer
    Summary of the data analysis workflow. (1) <t>High-resolution</t> metabolomics was performed on stored plasma EDTA samples; (2) an integrative network analysis of the body fat deposition, clinical biomarkers, and plasma metabolomics data sets was performed in xMWAS; (3) the m/z features in each community detected in the integrative network analysis were entered into untargeted pathway analysis using Mummichog; (4) associations selected in the integrative network analysis between the clinical and metabolomics variables and the body fat variables were further examined using multiple linear regression adjusted for potential confounders. BMI, body <t>mass</t> index; EDTA, ethylene diaminete traacetic acid; m/z , mass to charge ratio; PLS, partial least squares.
    High Resolution Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 115 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/high resolution mass spectrometer/product/Thermo Fisher
    Average 99 stars, based on 115 article reviews
    Price from $9.99 to $1999.99
    high resolution mass spectrometer - by Bioz Stars, 2020-09
    99/100 stars
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    99
    Thermo Fisher q exactive high field hf hybrid quadrupole orbitrap mass spectrometer
    Quality control after crosslink identification at a 5% link FDR. (a) Results from crosslinking HSA with sulfo-SDA acquired on an Q Exactive and an LTQ <t>Orbitrap</t> Velos mass spectrometer. The line at 25 Å indicates the distance cutoff for links classified as long-distance. The inlet shows the fraction of long-distance links (LDL) in each dataset. (b) Score comparison between within-distance and LDL. LDL showed no significant (ns) deviation from the within-distance links (two-sided Mann-Whitney-U-test at α = 0.05).
    Q Exactive High Field Hf Hybrid Quadrupole Orbitrap Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/q exactive high field hf hybrid quadrupole orbitrap mass spectrometer/product/Thermo Fisher
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    q exactive high field hf hybrid quadrupole orbitrap mass spectrometer - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    Image Search Results


    Deep single-run proteomics of cell lines and human ovarian cancer tissue. ( a ) Summary of the shotgun proteomics workflow for OvCa cellular models and high-grade serous ovarian cancer (HGSOC) tissues. Following lysis, protein purification, and tryptic digest, peptides were separated by ultra-high performance liquid chromatography and measured in single runs using a quadrupole Orbitrap mass spectrometer. Label-free proteome quantification was performed using the MaxQuant software environment. ( b ) Workflow reproducibility for cell lines ( n =30), HGSOC tissues ( n =8) and primary FTEC cells ( n =3). Pearson correlations ( r ) were calculated for biological replicates of cell lines, primary FTEC isolates from different healthy donors, and ovarian tumour tissues from both ovaries from a woman with HGSOC. ( c ) Average number of quantified proteins from each sample type. Error bars represent standard deviations. ( d ) Number of proteins common to FTECs, HGSOC tumours and cell lines. FTEC, fallopian tube epithelial cell; LC-MS/MS, liquid chromatography tandem mass spectrometry; m/z , mass-to-charge ratio; uHPLC, ultra-high performance liquid chromatography.

    Journal: Nature Communications

    Article Title: Integrative proteomic profiling of ovarian cancer cell lines reveals precursor cell associated proteins and functional status

    doi: 10.1038/ncomms12645

    Figure Lengend Snippet: Deep single-run proteomics of cell lines and human ovarian cancer tissue. ( a ) Summary of the shotgun proteomics workflow for OvCa cellular models and high-grade serous ovarian cancer (HGSOC) tissues. Following lysis, protein purification, and tryptic digest, peptides were separated by ultra-high performance liquid chromatography and measured in single runs using a quadrupole Orbitrap mass spectrometer. Label-free proteome quantification was performed using the MaxQuant software environment. ( b ) Workflow reproducibility for cell lines ( n =30), HGSOC tissues ( n =8) and primary FTEC cells ( n =3). Pearson correlations ( r ) were calculated for biological replicates of cell lines, primary FTEC isolates from different healthy donors, and ovarian tumour tissues from both ovaries from a woman with HGSOC. ( c ) Average number of quantified proteins from each sample type. Error bars represent standard deviations. ( d ) Number of proteins common to FTECs, HGSOC tumours and cell lines. FTEC, fallopian tube epithelial cell; LC-MS/MS, liquid chromatography tandem mass spectrometry; m/z , mass-to-charge ratio; uHPLC, ultra-high performance liquid chromatography.

    Article Snippet: Liquid chromatography-MS analysis MS analysis was performed using Quadrupole Orbitrap mass spectrometers (Q Exactive and Q Exactive HF, Thermo Fisher Scientific, Rockford, IL, USA) coupled to an EASY-nLC 1000 HPLC system (Thermo Fisher Scientific) via a nano electrospray source.

    Techniques: Lysis, Protein Purification, High Performance Liquid Chromatography, Mass Spectrometry, Software, Liquid Chromatography with Mass Spectroscopy, Liquid Chromatography

    Quality control after cross-link identification at a 5% link FDR. (a) Results from cross-linking HSA with sulfo-SDA acquired on an Q Exactive and an LTQ Orbitrap Velos mass spectrometer. The line at 25 Å indicates the distance cutoff for links classified as long distance. The inlet shows the fraction of long-distance links (LDL) in each data set. (b) Score comparison between within-distance linkzs and LDL. LDL showed no significant (ns) deviation from the within-distance links (two-sided Mann–Whitney-U-test at α = 0.05).

    Journal: Analytical Chemistry

    Article Title: Noncovalently Associated Peptides Observed during Liquid Chromatography-Mass Spectrometry and Their Effect on Cross-Link Analyses

    doi: 10.1021/acs.analchem.8b04037

    Figure Lengend Snippet: Quality control after cross-link identification at a 5% link FDR. (a) Results from cross-linking HSA with sulfo-SDA acquired on an Q Exactive and an LTQ Orbitrap Velos mass spectrometer. The line at 25 Å indicates the distance cutoff for links classified as long distance. The inlet shows the fraction of long-distance links (LDL) in each data set. (b) Score comparison between within-distance linkzs and LDL. LDL showed no significant (ns) deviation from the within-distance links (two-sided Mann–Whitney-U-test at α = 0.05).

    Article Snippet: The eluting peptides were sprayed into a Q Exactive High-field (HF) Hybrid Quadrupole-Orbitrap Mass Spectrometer (Thermo Fisher Scientific, Bremen, Germany).

    Techniques: Mass Spectrometry, MANN-WHITNEY

    Summary of the data analysis workflow. (1) High-resolution metabolomics was performed on stored plasma EDTA samples; (2) an integrative network analysis of the body fat deposition, clinical biomarkers, and plasma metabolomics data sets was performed in xMWAS; (3) the m/z features in each community detected in the integrative network analysis were entered into untargeted pathway analysis using Mummichog; (4) associations selected in the integrative network analysis between the clinical and metabolomics variables and the body fat variables were further examined using multiple linear regression adjusted for potential confounders. BMI, body mass index; EDTA, ethylene diaminete traacetic acid; m/z , mass to charge ratio; PLS, partial least squares.

    Journal: BMJ Open Diabetes Research & Care

    Article Title: Hepatic fat is a stronger correlate of key clinical and molecular abnormalities than visceral and abdominal subcutaneous fat in youth

    doi: 10.1136/bmjdrc-2019-001126

    Figure Lengend Snippet: Summary of the data analysis workflow. (1) High-resolution metabolomics was performed on stored plasma EDTA samples; (2) an integrative network analysis of the body fat deposition, clinical biomarkers, and plasma metabolomics data sets was performed in xMWAS; (3) the m/z features in each community detected in the integrative network analysis were entered into untargeted pathway analysis using Mummichog; (4) associations selected in the integrative network analysis between the clinical and metabolomics variables and the body fat variables were further examined using multiple linear regression adjusted for potential confounders. BMI, body mass index; EDTA, ethylene diaminete traacetic acid; m/z , mass to charge ratio; PLS, partial least squares.

    Article Snippet: Separate hydrophilic interaction liquid chromatography (HILIC) and C18 chromatography columns were coupled with detection by a high-resolution mass spectrometer (Q Exactive HF Orbitrap, Thermo Scientific, San Jose, California) operated in full scan mode at 120 000 resolution and mass to charge ratio (m/z) range 85–1275.

    Techniques:

    Quality control after crosslink identification at a 5% link FDR. (a) Results from crosslinking HSA with sulfo-SDA acquired on an Q Exactive and an LTQ Orbitrap Velos mass spectrometer. The line at 25 Å indicates the distance cutoff for links classified as long-distance. The inlet shows the fraction of long-distance links (LDL) in each dataset. (b) Score comparison between within-distance and LDL. LDL showed no significant (ns) deviation from the within-distance links (two-sided Mann-Whitney-U-test at α = 0.05).

    Journal: bioRxiv

    Article Title: Non-covalently-associated peptides are observed during liquid chromatography-mass spectrometry and affect crosslink analyses

    doi: 10.1101/502351

    Figure Lengend Snippet: Quality control after crosslink identification at a 5% link FDR. (a) Results from crosslinking HSA with sulfo-SDA acquired on an Q Exactive and an LTQ Orbitrap Velos mass spectrometer. The line at 25 Å indicates the distance cutoff for links classified as long-distance. The inlet shows the fraction of long-distance links (LDL) in each dataset. (b) Score comparison between within-distance and LDL. LDL showed no significant (ns) deviation from the within-distance links (two-sided Mann-Whitney-U-test at α = 0.05).

    Article Snippet: The eluting peptides were sprayed into a Q Exactive High-field (HF) Hybrid Quadrupole-Orbitrap Mass Spectrometer (Thermo Fisher Scientific, Bremen, Germany).

    Techniques: Mass Spectrometry, MANN-WHITNEY