orbitrap lc ms  (Thermo Fisher)


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    Structured Review

    Thermo Fisher orbitrap lc ms
    Orbitrap Lc Ms, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/orbitrap lc ms/product/Thermo Fisher
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    orbitrap lc ms - by Bioz Stars, 2020-07
    99/100 stars

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    Related Articles

    High Performance Liquid Chromatography:

    Article Title: Selenium species-dependent toxicity, bioavailability and metabolic transformations in Caenorhabditis elegans
    Article Snippet: .. For identification of selenium metabolites the HPLC system was coupled to a Q-Exactive Orbitrap Mass Spectrometer (Thermo Scientific, Waltham, USA) equipped with a heated electrospray ionization (HESI2) source from the same manufacturer. .. MS was performed in the positive mode and source settings were: gas temp 400°C, flow rates 47 (sheath) and 11 (aux) instrument units, spray voltage 3500 V and capillary temp 250°C.

    Liquid Chromatography:

    Article Title: AZA-MS: a novel multiparameter mass spectrometry method to determine the intracellular dynamics of azacitidine therapy in vivo
    Article Snippet: .. Mass spectrometry and data analysis LC–MS analysis was performed utilising an ultra-high-performance liquid chromatography system (Dionex u3000 system, Thermo Fisher Scientific) interfaced to an Orbitrap mass spectrometer (Q Exactive Plus, Thermo Fisher Scientific) using a heated electrospray interface operated in the positive ion mode. .. The chromatographic separation was performed on a 100 mm × 2.1 mm i.d., 3 μM , C30 column (Acclaim, Thermo Fisher Scientific) kept at 40 ºC.

    Mass Spectrometry:

    Article Title: AZA-MS: a novel multiparameter mass spectrometry method to determine the intracellular dynamics of azacitidine therapy in vivo
    Article Snippet: .. Mass spectrometry and data analysis LC–MS analysis was performed utilising an ultra-high-performance liquid chromatography system (Dionex u3000 system, Thermo Fisher Scientific) interfaced to an Orbitrap mass spectrometer (Q Exactive Plus, Thermo Fisher Scientific) using a heated electrospray interface operated in the positive ion mode. .. The chromatographic separation was performed on a 100 mm × 2.1 mm i.d., 3 μM , C30 column (Acclaim, Thermo Fisher Scientific) kept at 40 ºC.

    Article Title: Serial-omics of P53−/−, Brca1−/− Mouse Breast Tumor and Normal Mammary Gland
    Article Snippet: .. Lipids were analyzed using a high resolution hybrid QExactive Plus Orbitrap mass spectrometer (Thermo Fisher Scientific) in DDA mode (Top 8) using positive/negative ion polarity switching. ..

    Article Title: Selenium species-dependent toxicity, bioavailability and metabolic transformations in Caenorhabditis elegans
    Article Snippet: .. For identification of selenium metabolites the HPLC system was coupled to a Q-Exactive Orbitrap Mass Spectrometer (Thermo Scientific, Waltham, USA) equipped with a heated electrospray ionization (HESI2) source from the same manufacturer. .. MS was performed in the positive mode and source settings were: gas temp 400°C, flow rates 47 (sheath) and 11 (aux) instrument units, spray voltage 3500 V and capillary temp 250°C.

    Article Title: Quantitative Paper Spray Mass Spectrometry Analysis of Drugs of Abuse
    Article Snippet: .. The MS spectra were recorded using an Exactive Orbitrap mass spectrometer (Exactive, Thermo Scientific, CA). .. Quantitation of drugs in blood was carried out by tandem mass spectrometry (MS/MS) using a TSQ Quantum Access Max mass spectrometer (Thermo Scientific, CA) in the multiple reaction monitoring (MRM) mode with an isolation widow width of m/z 0.4.

    Article Title: Purification and Characterization of Latent Polyphenol Oxidase from Apricot (Prunus armeniaca L.)
    Article Snippet: .. Tryptic digestions of Pa PPO were analyzed by nanoUHPLC–ESI–MS/MS using a high-resolution Orbitrap mass spectrometer (Dionex Ultimate 3000 RSLCnano, Q Exactive Orbitrap, Thermo Scientific). .. The data analysis was performed with Proteome Discoverer 1.4 by searching against the P. armeniaca entries from the UniProt database (containing all listed proteins from P. mume , P. persica , and P. armeniaca ).

    Article Title: The emerging role of native mass spectrometry in characterizing the structure and dynamics of macromolecular complexes
    Article Snippet: .. In 2012, Heck et al . first demonstrated the use of an Orbitrap mass spectrometer (Exactive Plus™, Thermo Fisher Scientific) for the analysis of intact protein complexes ranging from 150 kDa to 800 kDa. .. – They subsequently extended this range to include virus-like nanoparticles with a mass up to 4.5 MDa ( ).

    Article Title: Application of high-resolution mass spectrometry to measure low abundance isotope enrichment in individual muscle proteins
    Article Snippet: .. Analysis of [ring-13 C6 ]-phenylalanine enrichment was performed with a high-resolution orbitrap mass spectrometer (Thermo Q Exactive Plus) using a 14 min targeted MS/MS method. .. The orbitrap MS was operated at 70,000 resolution, with an AGC target of 5e6 and maximum injection time of 100 ms. An isolation width of 1.0 m/z was applied for isolation of the m+3 and m+6 precursor species at m/z 225.15 and 228.17, respectively, and MS/MS was performed in the HCD cell at 40 V. All resulting fragments within the m/z 50-250 window were detected in the orbitrap.

    Article Title: Label-free and isobaric tandem mass tag (TMT) multiplexed quantitative proteomic data of two contrasting rice cultivars exposed to drought stress and recovery
    Article Snippet: .. These fractions were desalted using C18 OMIX® tips (Agilent) and analysed on a Q Exactive Orbitrap mass spectrometer (Thermo Scientific) coupled to an EASY-nLC1000 (Thermo Scientific). ..

    Liquid Chromatography with Mass Spectroscopy:

    Article Title: AZA-MS: a novel multiparameter mass spectrometry method to determine the intracellular dynamics of azacitidine therapy in vivo
    Article Snippet: .. Mass spectrometry and data analysis LC–MS analysis was performed utilising an ultra-high-performance liquid chromatography system (Dionex u3000 system, Thermo Fisher Scientific) interfaced to an Orbitrap mass spectrometer (Q Exactive Plus, Thermo Fisher Scientific) using a heated electrospray interface operated in the positive ion mode. .. The chromatographic separation was performed on a 100 mm × 2.1 mm i.d., 3 μM , C30 column (Acclaim, Thermo Fisher Scientific) kept at 40 ºC.

    Tandem Mass Spectroscopy:

    Article Title: Application of high-resolution mass spectrometry to measure low abundance isotope enrichment in individual muscle proteins
    Article Snippet: .. Analysis of [ring-13 C6 ]-phenylalanine enrichment was performed with a high-resolution orbitrap mass spectrometer (Thermo Q Exactive Plus) using a 14 min targeted MS/MS method. .. The orbitrap MS was operated at 70,000 resolution, with an AGC target of 5e6 and maximum injection time of 100 ms. An isolation width of 1.0 m/z was applied for isolation of the m+3 and m+6 precursor species at m/z 225.15 and 228.17, respectively, and MS/MS was performed in the HCD cell at 40 V. All resulting fragments within the m/z 50-250 window were detected in the orbitrap.

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    Thermo Fisher q exactive orbitrap lc ms ms
    Principal component loading plot and scores of principal component analysis (PCA) of the concentrations of 13 key phenolic compounds and total polyphenols identified and quantitated by <t>UHPLC-Q-Orbitrap</t> HRMS analysis in four microgreens genotypes as modulated by variable spectral bandwidths in a controlled growth environment.
    Q Exactive Orbitrap Lc Ms Ms, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/q exactive orbitrap lc ms ms/product/Thermo Fisher
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    q exactive orbitrap lc ms ms - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    90
    Thermo Fisher lc esi orbitrap ms
    Identification of polyphenolic compounds in E. characias leaves using <t>LC-ESI-Orbitrap-MS/MS</t> in negative ion mode. Chromatographic conditions are described in the text. A list of compounds is reported in Table 5 .
    Lc Esi Orbitrap Ms, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lc esi orbitrap ms/product/Thermo Fisher
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    lc esi orbitrap ms - by Bioz Stars, 2020-07
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    86
    Thermo Fisher orbitrap elite lc ms ms instrument
    Identification of pKpQIL_p019 protein using three proteomics methods. (A) N-terminal Edman sequencing was used to define a 26-amino-acid sequence from the purified 11,109-Da protein (blue). Protein BLAST identified this sequence as a fragment representing amino acids 21 to 46 of the 119-amino-acid protein pKpQIL_p019, encoded by the pKpQIL plasmid. The mass of amino acids 21 to 119 (red) is 11,107.8 Da, corresponding to the identified MALDI-TOF MS peak (note that only a partial protein sequence is shown). The first 20 amino acids (green) are predicted to represent a signal peptide with a cleavage site between amino acids 20 and 21. (B) Top-down proteomics analysis was performed using CID by Thermo <t>Orbitrap</t> Elite. LC–MS-MS confirmed the identity of the protein as pKpQIL_p019. Cleavage sites used for identification are shown in purple. A total of 13 b-ion fragments and 15 y-ion fragments were detected. (C) Bottom-up proteomics performed using trypsin digestion also confirmed the identity of the protein as pKpQIL_p019. Tryptic digest analysis yielded 89% coverage (shown in red) of the purified protein cleavage fragment (amino acids 21 to 119) with a total Mascot score of 4,600 at a 1% false discovery rate (FDR).
    Orbitrap Elite Lc Ms Ms Instrument, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/orbitrap elite lc ms ms instrument/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    orbitrap elite lc ms ms instrument - by Bioz Stars, 2020-07
    86/100 stars
      Buy from Supplier

    Image Search Results


    Principal component loading plot and scores of principal component analysis (PCA) of the concentrations of 13 key phenolic compounds and total polyphenols identified and quantitated by UHPLC-Q-Orbitrap HRMS analysis in four microgreens genotypes as modulated by variable spectral bandwidths in a controlled growth environment.

    Journal: Frontiers in Plant Science

    Article Title: Genotype-Specific Modulatory Effects of Select Spectral Bandwidths on the Nutritive and Phytochemical Composition of Microgreens

    doi: 10.3389/fpls.2019.01501

    Figure Lengend Snippet: Principal component loading plot and scores of principal component analysis (PCA) of the concentrations of 13 key phenolic compounds and total polyphenols identified and quantitated by UHPLC-Q-Orbitrap HRMS analysis in four microgreens genotypes as modulated by variable spectral bandwidths in a controlled growth environment.

    Article Snippet: Mass spectrometry analysis was facilitated by a Q Exactive Orbitrap LC-MS/MS (Thermo Fisher Scientific, Waltham, MA, USA).

    Techniques:

    Principal component loading plot and scores of PCA of phenolic compounds quantified by HUPLC-Q-Orbitrap HRMS analysis in green and red microgreens and mature leaves of Salanova lettuce.

    Journal: Antioxidants

    Article Title: Variation in Macronutrient Content, Phytochemical Constitution and In Vitro Antioxidant Capacity of Green and Red Butterhead Lettuce Dictated by Different Developmental Stages of Harvest Maturity

    doi: 10.3390/antiox9040300

    Figure Lengend Snippet: Principal component loading plot and scores of PCA of phenolic compounds quantified by HUPLC-Q-Orbitrap HRMS analysis in green and red microgreens and mature leaves of Salanova lettuce.

    Article Snippet: The accuracy and calibration of the Q Exactive Orbitrap LC-MS/MS was monitored by using a Thermo Fisher Scientific reference standard mixture.

    Techniques:

    Identification of polyphenolic compounds in E. characias leaves using LC-ESI-Orbitrap-MS/MS in negative ion mode. Chromatographic conditions are described in the text. A list of compounds is reported in Table 5 .

    Journal: BioMed Research International

    Article Title: Biological Activities of Aerial Parts Extracts of Euphorbia characias

    doi: 10.1155/2016/1538703

    Figure Lengend Snippet: Identification of polyphenolic compounds in E. characias leaves using LC-ESI-Orbitrap-MS/MS in negative ion mode. Chromatographic conditions are described in the text. A list of compounds is reported in Table 5 .

    Article Snippet: 2.7.1. (HR) LC-ESI-Orbitrap-MS and (HR) LC-ESI-Orbitrap-MS/MS The electrospray ionisation (ESI) source of a Thermo Scientific LTQ-Orbitrap XL (Thermo Scientific, Germany) mass spectrometer was tuned in negative ion mode with a standard solution of kaempferol-3-O -glucoside (1 μ g/mL) infused at a flow rate of 5 μ L/min with a syringe pump.

    Techniques: Mass Spectrometry

    Identification of pKpQIL_p019 protein using three proteomics methods. (A) N-terminal Edman sequencing was used to define a 26-amino-acid sequence from the purified 11,109-Da protein (blue). Protein BLAST identified this sequence as a fragment representing amino acids 21 to 46 of the 119-amino-acid protein pKpQIL_p019, encoded by the pKpQIL plasmid. The mass of amino acids 21 to 119 (red) is 11,107.8 Da, corresponding to the identified MALDI-TOF MS peak (note that only a partial protein sequence is shown). The first 20 amino acids (green) are predicted to represent a signal peptide with a cleavage site between amino acids 20 and 21. (B) Top-down proteomics analysis was performed using CID by Thermo Orbitrap Elite. LC–MS-MS confirmed the identity of the protein as pKpQIL_p019. Cleavage sites used for identification are shown in purple. A total of 13 b-ion fragments and 15 y-ion fragments were detected. (C) Bottom-up proteomics performed using trypsin digestion also confirmed the identity of the protein as pKpQIL_p019. Tryptic digest analysis yielded 89% coverage (shown in red) of the purified protein cleavage fragment (amino acids 21 to 119) with a total Mascot score of 4,600 at a 1% false discovery rate (FDR).

    Journal: Journal of Clinical Microbiology

    Article Title: A Rapid Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry-Based Method for Single-Plasmid Tracking in an Outbreak of Carbapenem-Resistant Enterobacteriaceae

    doi: 10.1128/JCM.00694-14

    Figure Lengend Snippet: Identification of pKpQIL_p019 protein using three proteomics methods. (A) N-terminal Edman sequencing was used to define a 26-amino-acid sequence from the purified 11,109-Da protein (blue). Protein BLAST identified this sequence as a fragment representing amino acids 21 to 46 of the 119-amino-acid protein pKpQIL_p019, encoded by the pKpQIL plasmid. The mass of amino acids 21 to 119 (red) is 11,107.8 Da, corresponding to the identified MALDI-TOF MS peak (note that only a partial protein sequence is shown). The first 20 amino acids (green) are predicted to represent a signal peptide with a cleavage site between amino acids 20 and 21. (B) Top-down proteomics analysis was performed using CID by Thermo Orbitrap Elite. LC–MS-MS confirmed the identity of the protein as pKpQIL_p019. Cleavage sites used for identification are shown in purple. A total of 13 b-ion fragments and 15 y-ion fragments were detected. (C) Bottom-up proteomics performed using trypsin digestion also confirmed the identity of the protein as pKpQIL_p019. Tryptic digest analysis yielded 89% coverage (shown in red) of the purified protein cleavage fragment (amino acids 21 to 119) with a total Mascot score of 4,600 at a 1% false discovery rate (FDR).

    Article Snippet: The digest was analyzed on a Thermo Orbitrap Elite LC–MS-MS instrument (Thermo Fisher Scientific, San Jose, CA) coupled with an Eksigent nanoLC-Ultra 1D plus system (Dublin, CA).

    Techniques: Sequencing, Purification, Plasmid Preparation, Mass Spectrometry, Liquid Chromatography with Mass Spectroscopy