orbitrap fusion lumos tribrid mass spectrometer lc ms  (Thermo Fisher)


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    Name:
    Orbitrap Fusion Lumos Tribrid Mass Spectrometer
    Description:
    Excel at the most challenging of applications including low level PTM analysis multiplexed relative quantitation using isobaric tags intact protein characterization as well as MSn analysis of small molecules with the new Thermo Scientific Orbitrap Fusion Lumos Tribrid Mass Spectrometer This system incorporates the brightest ion source a segmented quadrupole mass filter with improved selectivity and ion transmission Advanced Vacuum Technology for improved ion transmission to the Thermo Scientific Orbitrap mass analyzer and ETD HD a higher capacity ETD fragmentation Latest innovations include the Advanced Peak Determination APD algorithm for improved data dependent experiments and two new hardware options UVPD a new fragmentation technique achieved with a 213 nm UV laser and 1M which provides 1 000 000 FWHM ultra high resolution for improved structural elucidation and quantitation of isobaric compounds
    Catalog Number:
    iqlaaegaapfadbmbhq
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    None
    Applications:
    Industrial & Applied Science|Industrial Mass Spectrometry
    Category:
    Instruments and Equipment
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    Structured Review

    Thermo Fisher orbitrap fusion lumos tribrid mass spectrometer lc ms
    Excel at the most challenging of applications including low level PTM analysis multiplexed relative quantitation using isobaric tags intact protein characterization as well as MSn analysis of small molecules with the new Thermo Scientific Orbitrap Fusion Lumos Tribrid Mass Spectrometer This system incorporates the brightest ion source a segmented quadrupole mass filter with improved selectivity and ion transmission Advanced Vacuum Technology for improved ion transmission to the Thermo Scientific Orbitrap mass analyzer and ETD HD a higher capacity ETD fragmentation Latest innovations include the Advanced Peak Determination APD algorithm for improved data dependent experiments and two new hardware options UVPD a new fragmentation technique achieved with a 213 nm UV laser and 1M which provides 1 000 000 FWHM ultra high resolution for improved structural elucidation and quantitation of isobaric compounds
    https://www.bioz.com/result/orbitrap fusion lumos tribrid mass spectrometer lc ms/product/Thermo Fisher
    Average 90 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    orbitrap fusion lumos tribrid mass spectrometer lc ms - by Bioz Stars, 2020-02
    90/100 stars

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    Related Articles

    High Performance Liquid Chromatography:

    Article Title: Early urine proteome changes in the Walker-256 tail-vein injection rat model
    Article Snippet: Digested peptides were redissolved in 0.1% formic acid to a concentration of 0.5 μ g/μ L. For analysis, 1 μ g of each peptide from an individual sample was loaded onto a trap column and separated on a reverse-phase C18 column (50 μ m × 150 mm, 2 μ m) using the EASY-nLC 1200 HPLC system (Thermo Fisher Scientific, Waltham, MA) . .. Peptides were analyzed with an Orbitrap Fusion Lumos Tribrid mass spectrometer (Thermo Fisher Scientific, Waltham, MA).

    Article Title: Phosphorylation Changes in Response to Kinase Inhibitor H89 in PKA-Null Cells
    Article Snippet: Total and phosphopeptides were analyzed using a Dionex UltiMate 3000 nano LC system connected to an Orbitrap Fusion Lumos ETD mass spectrometer equipped with an EASY-Spray ion source (Thermo Fisher Scientific). .. Total and phosphopeptides were analyzed using a Dionex UltiMate 3000 nano LC system connected to an Orbitrap Fusion Lumos ETD mass spectrometer equipped with an EASY-Spray ion source (Thermo Fisher Scientific).

    Article Title: A Study into the ADP-Ribosylome of IFN-γ-Stimulated THP-1 Human Macrophage-like Cells Identifies ARTD8/PARP14 and ARTD9/PARP9 ADP-Ribosylation
    Article Snippet: .. LC–MS/MS Analysis All peptide samples were analyzed on an Orbitrap Fusion Lumos mass spectrometer fronted with an EASY-Spray Source, coupled to an Easy-nLC1000 HPLC pump (Thermo Fisher Scientific). .. The peptides were subjected to a dual column setup: an Acclaim PepMap RSLC C18 trap column, 75 μm × 20 mm (Thermo Fisher Scientific, Cat# 164261); and an EASY-Spray LC Column, 75 μm × 250 mm (Thermo Fisher Scientific, Cat# ES802).

    Article Title: Early urine proteome changes in the Walker-256 tail-vein injection rat model
    Article Snippet: .. PRM samples were analyzed by the EASY-nLC 1200 HPLC system (Thermo Fisher Scientific, Waltham, MA) coupled to the Orbitrap Fusion Lumos Tribrid mass spectrometer (Thermo Fisher Scientific, Waltham, MA). ..

    Flow Cytometry:

    Article Title: Early urine proteome changes in the Walker-256 tail-vein injection rat model
    Article Snippet: The elution for the analytical column lasted 120 min with a gradient of 5%–28% buffer B (0.1% formic acid in 80% acetonitrile; flow rate 0.3 μl/min). .. Peptides were analyzed with an Orbitrap Fusion Lumos Tribrid mass spectrometer (Thermo Fisher Scientific, Waltham, MA).

    Article Title: Isobaric tag-based protein profiling of a nicotine-treated alpha7 nicotinic receptor-null human haploid cell line
    Article Snippet: All samples were analyzed on an Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific, San Jose, CA) coupled to a Proxeon EASY-nLC 1200 liquid chromatography (LC) pump (Thermo Fisher Scientific). .. Peptides were separated using a 150min gradient of 3 to 25% acetonitrile in 0.125% formic acid with a flow rate of 450 nL/min.

    Article Title: Advancing a High Throughput Glycotope-centric Glycomics Workflow Based on nanoLC-MS2-product Dependent-MS3 Analysis of Permethylated Glycans* *
    Article Snippet: .. Another EASY-nLC™ 1200 system was interfaced to an Orbitrap Fusion™ Lumos™ Tribrid™ Mass Spectrometer (ThermoFisher Scientific) via a Nanospray Flex™ Ion Sources (ThermoFisher Scientific) for nanoLC separation at 50 °C using the same 25 cm x 75 μm C18 column (Acclaim PepMap® RSLC, ThermoFisher Scientific) at a constant flow rate of 300 nL/min. ..

    Article Title: PepSAVI-MS reveals anticancer and antifungal cycloviolacins in Viola odorata
    Article Snippet: Mobile phase A consisted of 0.1% formic acid, and mobile phase B consisted of 100% acetonitrile and 0.1% formic acid at a net flow rate of 300 nL/min. .. The chromatography system was coupled to Thermo Scientific Orbitrap Fusion Lumos mass spectrometer (San Jose, CA, USA) outfitted with a 193 nm excimer laser (Coherent Excistar 500 Hz, Santa Clara, CA, USA) to allow photodissociation, as implemented and described previously( ).

    Article Title: Phosphorylation Changes in Response to Kinase Inhibitor H89 in PKA-Null Cells
    Article Snippet: Total and phosphopeptides were analyzed using a Dionex UltiMate 3000 nano LC system connected to an Orbitrap Fusion Lumos ETD mass spectrometer equipped with an EASY-Spray ion source (Thermo Fisher Scientific). .. Peptides were introduced into a peptide nanotrap at a flow rate of 5 μL/min.

    Article Title: Improved Method for Determining Absolute Phosphorylation Stoichiometry Using Bayesian Statistics and Isobaric Labeling
    Article Snippet: Data for all LC–MS/MS experiments were collected on an Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific, San Jose, CA) with LC separation performed on an attached Proxeon EASY-nLC 1200 liquid chromatography (LC) pump (Thermo Fisher Scientific). .. A 150 min gradient of 6% to 25% acetonitrile in 0.125% formic acid was used at a flow rate of ~450 nL/min to separate peptides from the pooled TMT-labeled samples: MS1 spectra (Orbitrap resolution, 120 000; mass range, 350–1400 m/z ; automatic gain control (AGC) target, 5 × 105 ; maximum injection time, 100 ms).

    Article Title: Early urine proteome changes in the Walker-256 tail-vein injection rat model
    Article Snippet: PRM samples were analyzed by the EASY-nLC 1200 HPLC system (Thermo Fisher Scientific, Waltham, MA) coupled to the Orbitrap Fusion Lumos Tribrid mass spectrometer (Thermo Fisher Scientific, Waltham, MA). .. The elution lasted 120 min with 5%–28% buffer B (0.1% formic acid in 80% acetonitrile; flow rate 0.3 μl/min).

    Affinity Chromatography:

    Article Title: Phosphorylation Changes in Response to Kinase Inhibitor H89 in PKA-Null Cells
    Article Snippet: Samples were combined and, after taking an aliquot for total proteomics, phosphopeptides were sequentially enriched following Sequential Enrichment from Metal Oxide Affinity Chromatography protocol (SMOAC, Thermo Scientific). .. Total and phosphopeptides were analyzed using a Dionex UltiMate 3000 nano LC system connected to an Orbitrap Fusion Lumos ETD mass spectrometer equipped with an EASY-Spray ion source (Thermo Fisher Scientific).

    Sample Prep:

    Article Title: Phosphorylation Changes in Response to Kinase Inhibitor H89 in PKA-Null Cells
    Article Snippet: Paragraph title: Sample preparation for total- and phospho-proteomics ... Total and phosphopeptides were analyzed using a Dionex UltiMate 3000 nano LC system connected to an Orbitrap Fusion Lumos ETD mass spectrometer equipped with an EASY-Spray ion source (Thermo Fisher Scientific).

    Mass Spectrometry:

    Article Title: Benchmarking common quantification strategies for large-scale phosphoproteomics
    Article Snippet: .. Nanoflow LC tandem MS All samples were analyzed on an Easy-nLC 1000 coupled to a Q-Exactive HF instrument (Thermo Fisher Scientific; TMT MS2 of Fig. ), an Orbitrap Fusion Lumos instrument (Thermo Fisher Scientific; Figs. , , TMT MS3 of 3, , Supplementary Fig. ), or a Q-Exactive HF-X instrument (Thermo Fisher Scientific; Fig. ), all equipped with a nanoelectrospray source. ..

    Article Title: Early urine proteome changes in the Walker-256 tail-vein injection rat model
    Article Snippet: .. Peptides were analyzed with an Orbitrap Fusion Lumos Tribrid mass spectrometer (Thermo Fisher Scientific, Waltham, MA). .. MS data were acquired in high-sensitivity mode using the following parameters: data-dependent MS/MS scans per full scan with top-speed mode (3 s), MS scans at a resolution of 120,000 and MS/MS scans at a resolution of 30,000 in the Orbitrap, 30% HCD collision energy, charge-state screening (+2 to +7), dynamic exclusion (exclusion duration 30 s), and a maximum injection time of 45 ms .

    Article Title: Isobaric tag-based protein profiling of a nicotine-treated alpha7 nicotinic receptor-null human haploid cell line
    Article Snippet: .. All samples were analyzed on an Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific, San Jose, CA) coupled to a Proxeon EASY-nLC 1200 liquid chromatography (LC) pump (Thermo Fisher Scientific). .. Peptides were separated on a 100 μm inner diameter microcapillary column packed with 35 cm of Accucore C18 resin (2.6 μm, 150 Å, ThermoFisher).

    Article Title: Increased N,N-Dimethyl Leucine Isobaric Tag Multiplexing by a Combined Precursor Isotopic Labeling and Isobaric Tagging Approach
    Article Snippet: .. Samples were analyzed by nanoLC-MS/MS using a Dionex UltiMate 3000 UPLC system coupled to a Thermo Scientific Orbitrap Fusion Lumos mass spectrometer. .. Labeled peptide samples were dried in vacuo and dissolved in 3% ACN, 0.1% formic acid in water.

    Article Title: T-cell immunopeptidomes reveal cell subtype surface markers derived from intracellular proteins
    Article Snippet: .. Isolated HLA peptides were reconstituted in 10-12 μl of 0.1 % formic acid and analyzed on a LTQ Orbitrap Elite mass spectrometer (Thermo Fisher Scientific, Bremen, Germany) or a Fusion Lumos mass spectrometer (Thermo Fisher Scientific, San Jose, USA). .. Peptides were separated by capillary reverse phase chromatography on a 20 cm reversed phase column (100 μm inner diameter, packed in-house with ReproSil-Pur C18-AQ 3.0 m resin (Dr. Maisch GmbH)).

    Article Title: Enzyme reversal to explore the function of yeast E3 ubiquitin-ligases
    Article Snippet: .. × 10 cm using the Thermo EZ nLC 1200 and analyzed on an Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific, San Jose, CA). .. For each injection, an estimated 0.5 μg of total peptide was loaded and followed though a 120 minute linear gradient of 0% to 95% ACN in 0.1% Formic acid.

    Article Title: Advancing a High Throughput Glycotope-centric Glycomics Workflow Based on nanoLC-MS2-product Dependent-MS3 Analysis of Permethylated Glycans* *
    Article Snippet: .. Another EASY-nLC™ 1200 system was interfaced to an Orbitrap Fusion™ Lumos™ Tribrid™ Mass Spectrometer (ThermoFisher Scientific) via a Nanospray Flex™ Ion Sources (ThermoFisher Scientific) for nanoLC separation at 50 °C using the same 25 cm x 75 μm C18 column (Acclaim PepMap® RSLC, ThermoFisher Scientific) at a constant flow rate of 300 nL/min. ..

    Article Title: PepSAVI-MS reveals anticancer and antifungal cycloviolacins in Viola odorata
    Article Snippet: .. The chromatography system was coupled to Thermo Scientific Orbitrap Fusion Lumos mass spectrometer (San Jose, CA, USA) outfitted with a 193 nm excimer laser (Coherent Excistar 500 Hz, Santa Clara, CA, USA) to allow photodissociation, as implemented and described previously( ). ..

    Article Title: Leishmania major degrades murine CXCL1 – An immune evasion strategy
    Article Snippet: .. The combined extracts were concentrated in a lyophilizer and rehydrated with 15μl of mobile phase A. LC-MS/MS: Mass spectrometry data were collected using an Orbitrap Fusion Lumos mass spectrometer (Thermofisher) coupled to an Easy-nLC-120 System (Proxeon P/N LC1400). .. The autosampler is set to aspirate 3μl (estimated 0.3ug) of reconstituted digest and load the solution on a 2.5cm C18 trap (New Objective, P/N IT100-25H002) coupled to waste, HV or analytical column through a microcross assembly (IDEX, P/N UH-752).

    Article Title: Comparative bioinformatic analysis suggests that specific dauer-like signalling pathway components regulate Toxocara canis development and migration in the mammalian host
    Article Snippet: .. The organic phase was retained, dried and resuspended in methanol for subsequent mass spectrometric analysis using an Orbitrap Fusion Lumos mass spectrometer coupled to an Ultimate 3000 UHPLC (Thermo Fisher Scientific, San Jose, CA, USA). .. Commercially available dafachronic acids (25S)-Δ7-DA and (25S)-Δ4-DA (exact mass: 413.3061) (cat no. 23017-97-2; Cayman Chemical Company) were used as reference standards for the identification of endogenous DAs.

    Article Title: Targeted MultiNotch MS3 Approach for Relative Quantification of N-Glycans Using Multiplexed Carbonyl-Reactive Isobaric Tags
    Article Snippet: .. An Orbitrap Fusion Lumos Tribrid quadrupole-ion trap-Orbitrap mass spectrometer with NanoSpray Flex ion source (Thermo Scientific, Bremen, Germany) was used for data acquisition. ..

    Article Title: Phosphorylation Changes in Response to Kinase Inhibitor H89 in PKA-Null Cells
    Article Snippet: .. Total and phosphopeptides were analyzed using a Dionex UltiMate 3000 nano LC system connected to an Orbitrap Fusion Lumos ETD mass spectrometer equipped with an EASY-Spray ion source (Thermo Fisher Scientific). .. Peptides were introduced into a peptide nanotrap at a flow rate of 5 μL/min.

    Article Title: A Study into the ADP-Ribosylome of IFN-γ-Stimulated THP-1 Human Macrophage-like Cells Identifies ARTD8/PARP14 and ARTD9/PARP9 ADP-Ribosylation
    Article Snippet: .. LC–MS/MS Analysis All peptide samples were analyzed on an Orbitrap Fusion Lumos mass spectrometer fronted with an EASY-Spray Source, coupled to an Easy-nLC1000 HPLC pump (Thermo Fisher Scientific). .. The peptides were subjected to a dual column setup: an Acclaim PepMap RSLC C18 trap column, 75 μm × 20 mm (Thermo Fisher Scientific, Cat# 164261); and an EASY-Spray LC Column, 75 μm × 250 mm (Thermo Fisher Scientific, Cat# ES802).

    Article Title: Improved Method for Determining Absolute Phosphorylation Stoichiometry Using Bayesian Statistics and Isobaric Labeling
    Article Snippet: .. Data for all LC–MS/MS experiments were collected on an Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific, San Jose, CA) with LC separation performed on an attached Proxeon EASY-nLC 1200 liquid chromatography (LC) pump (Thermo Fisher Scientific). ..

    Article Title: Early urine proteome changes in the Walker-256 tail-vein injection rat model
    Article Snippet: .. PRM samples were analyzed by the EASY-nLC 1200 HPLC system (Thermo Fisher Scientific, Waltham, MA) coupled to the Orbitrap Fusion Lumos Tribrid mass spectrometer (Thermo Fisher Scientific, Waltham, MA). ..

    Chromatography:

    Article Title: PepSAVI-MS reveals anticancer and antifungal cycloviolacins in Viola odorata
    Article Snippet: .. The chromatography system was coupled to Thermo Scientific Orbitrap Fusion Lumos mass spectrometer (San Jose, CA, USA) outfitted with a 193 nm excimer laser (Coherent Excistar 500 Hz, Santa Clara, CA, USA) to allow photodissociation, as implemented and described previously( ). ..

    Article Title: Comparative bioinformatic analysis suggests that specific dauer-like signalling pathway components regulate Toxocara canis development and migration in the mammalian host
    Article Snippet: Paragraph title: Liquid chromatography-mass spectrometry (LC-MS) ... The organic phase was retained, dried and resuspended in methanol for subsequent mass spectrometric analysis using an Orbitrap Fusion Lumos mass spectrometer coupled to an Ultimate 3000 UHPLC (Thermo Fisher Scientific, San Jose, CA, USA).

    Isolation:

    Article Title: T-cell immunopeptidomes reveal cell subtype surface markers derived from intracellular proteins
    Article Snippet: .. Isolated HLA peptides were reconstituted in 10-12 μl of 0.1 % formic acid and analyzed on a LTQ Orbitrap Elite mass spectrometer (Thermo Fisher Scientific, Bremen, Germany) or a Fusion Lumos mass spectrometer (Thermo Fisher Scientific, San Jose, USA). .. Peptides were separated by capillary reverse phase chromatography on a 20 cm reversed phase column (100 μm inner diameter, packed in-house with ReproSil-Pur C18-AQ 3.0 m resin (Dr. Maisch GmbH)).

    Article Title: Enzyme reversal to explore the function of yeast E3 ubiquitin-ligases
    Article Snippet: × 10 cm using the Thermo EZ nLC 1200 and analyzed on an Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific, San Jose, CA). .. The most abundant precursors were selected among 2-8 charge state ions at a 2.0E5 threshold, isolated by a multi-segment quadrupole with a mass window of m/z 2, and sequentially subjected to both CID and HCD fragmentation.

    Article Title: PepSAVI-MS reveals anticancer and antifungal cycloviolacins in Viola odorata
    Article Snippet: The chromatography system was coupled to Thermo Scientific Orbitrap Fusion Lumos mass spectrometer (San Jose, CA, USA) outfitted with a 193 nm excimer laser (Coherent Excistar 500 Hz, Santa Clara, CA, USA) to allow photodissociation, as implemented and described previously( ). .. The MS/MS spectra were collected using the following parameters: quadrupole isolation with an isolation width of 4 m/z; 15000 FT resolving power; 2 × 105 AGC target; 2 µscans averaged per spectrum.

    Centrifugation:

    Article Title: Comparative bioinformatic analysis suggests that specific dauer-like signalling pathway components regulate Toxocara canis development and migration in the mammalian host
    Article Snippet: A chloroform:methanol (2:1) mix was used to separate the aqueous and organic phases by centrifugation at 10,000× g for 10 min at room temperature (24 °C). .. The organic phase was retained, dried and resuspended in methanol for subsequent mass spectrometric analysis using an Orbitrap Fusion Lumos mass spectrometer coupled to an Ultimate 3000 UHPLC (Thermo Fisher Scientific, San Jose, CA, USA).

    Labeling:

    Article Title: Increased N,N-Dimethyl Leucine Isobaric Tag Multiplexing by a Combined Precursor Isotopic Labeling and Isobaric Tagging Approach
    Article Snippet: Samples were analyzed by nanoLC-MS/MS using a Dionex UltiMate 3000 UPLC system coupled to a Thermo Scientific Orbitrap Fusion Lumos mass spectrometer. .. Labeled peptide samples were dried in vacuo and dissolved in 3% ACN, 0.1% formic acid in water.

    Article Title: Enzyme reversal to explore the function of yeast E3 ubiquitin-ligases
    Article Snippet: Each labeled (‘light’ and ‘heavy’) peptide mixture was loaded on home-packed C-18 column (Halo 2.7 μm particles: MDC) 100 μm i.d. .. × 10 cm using the Thermo EZ nLC 1200 and analyzed on an Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific, San Jose, CA).

    Liquid Chromatography:

    Article Title: Isobaric tag-based protein profiling of a nicotine-treated alpha7 nicotinic receptor-null human haploid cell line
    Article Snippet: .. All samples were analyzed on an Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific, San Jose, CA) coupled to a Proxeon EASY-nLC 1200 liquid chromatography (LC) pump (Thermo Fisher Scientific). .. Peptides were separated on a 100 μm inner diameter microcapillary column packed with 35 cm of Accucore C18 resin (2.6 μm, 150 Å, ThermoFisher).

    Article Title: Improved Method for Determining Absolute Phosphorylation Stoichiometry Using Bayesian Statistics and Isobaric Labeling
    Article Snippet: .. Data for all LC–MS/MS experiments were collected on an Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific, San Jose, CA) with LC separation performed on an attached Proxeon EASY-nLC 1200 liquid chromatography (LC) pump (Thermo Fisher Scientific). ..

    Concentration Assay:

    Article Title: Early urine proteome changes in the Walker-256 tail-vein injection rat model
    Article Snippet: Digested peptides were redissolved in 0.1% formic acid to a concentration of 0.5 μ g/μ L. For analysis, 1 μ g of each peptide from an individual sample was loaded onto a trap column and separated on a reverse-phase C18 column (50 μ m × 150 mm, 2 μ m) using the EASY-nLC 1200 HPLC system (Thermo Fisher Scientific, Waltham, MA) . .. Peptides were analyzed with an Orbitrap Fusion Lumos Tribrid mass spectrometer (Thermo Fisher Scientific, Waltham, MA).

    Article Title: PepSAVI-MS reveals anticancer and antifungal cycloviolacins in Viola odorata
    Article Snippet: 1 µL of sample at a concentration of approximately 1 mM total protein content was injected on column per experiment. .. The chromatography system was coupled to Thermo Scientific Orbitrap Fusion Lumos mass spectrometer (San Jose, CA, USA) outfitted with a 193 nm excimer laser (Coherent Excistar 500 Hz, Santa Clara, CA, USA) to allow photodissociation, as implemented and described previously( ).

    Liquid Chromatography with Mass Spectroscopy:

    Article Title: Comparative bioinformatic analysis suggests that specific dauer-like signalling pathway components regulate Toxocara canis development and migration in the mammalian host
    Article Snippet: Paragraph title: Liquid chromatography-mass spectrometry (LC-MS) ... The organic phase was retained, dried and resuspended in methanol for subsequent mass spectrometric analysis using an Orbitrap Fusion Lumos mass spectrometer coupled to an Ultimate 3000 UHPLC (Thermo Fisher Scientific, San Jose, CA, USA).

    Article Title: Phosphorylation Changes in Response to Kinase Inhibitor H89 in PKA-Null Cells
    Article Snippet: The dried peptides were re-suspended with 0.1% formic acid in LC-MS grade water (J.T. .. Total and phosphopeptides were analyzed using a Dionex UltiMate 3000 nano LC system connected to an Orbitrap Fusion Lumos ETD mass spectrometer equipped with an EASY-Spray ion source (Thermo Fisher Scientific).

    Tandem Mass Spectroscopy:

    Article Title: Early urine proteome changes in the Walker-256 tail-vein injection rat model
    Article Snippet: The MS data were acquired in high-sensitivity mode using the following parameters: 30 data-dependent MS/MS scans per full scan, full scans acquired at a resolution of 40,000 and MS/MS scans at a resolution of 20,000, rolling collision energy, charge-state screening (+2 to +5) , dynamic exclusion (exclusion duration 30 s), an MS/MS scan range of 250–1800 m/z, and a scan time of 50 ms. Each sample was analyzed twice (2 μg each analysis). .. Peptides were analyzed with an Orbitrap Fusion Lumos Tribrid mass spectrometer (Thermo Fisher Scientific, Waltham, MA).

    Article Title: PepSAVI-MS reveals anticancer and antifungal cycloviolacins in Viola odorata
    Article Snippet: The chromatography system was coupled to Thermo Scientific Orbitrap Fusion Lumos mass spectrometer (San Jose, CA, USA) outfitted with a 193 nm excimer laser (Coherent Excistar 500 Hz, Santa Clara, CA, USA) to allow photodissociation, as implemented and described previously( ). .. The MS/MS spectra were collected using the following parameters: quadrupole isolation with an isolation width of 4 m/z; 15000 FT resolving power; 2 × 105 AGC target; 2 µscans averaged per spectrum.

    Modification:

    Article Title: Leishmania major degrades murine CXCL1 – An immune evasion strategy
    Article Snippet: A procedure slightly modified than that described by Yu et al [ ] was used for in-gel digestion. .. The combined extracts were concentrated in a lyophilizer and rehydrated with 15μl of mobile phase A. LC-MS/MS: Mass spectrometry data were collected using an Orbitrap Fusion Lumos mass spectrometer (Thermofisher) coupled to an Easy-nLC-120 System (Proxeon P/N LC1400).

    Reversed-phase Chromatography:

    Article Title: T-cell immunopeptidomes reveal cell subtype surface markers derived from intracellular proteins
    Article Snippet: Isolated HLA peptides were reconstituted in 10-12 μl of 0.1 % formic acid and analyzed on a LTQ Orbitrap Elite mass spectrometer (Thermo Fisher Scientific, Bremen, Germany) or a Fusion Lumos mass spectrometer (Thermo Fisher Scientific, San Jose, USA). .. Isolated HLA peptides were reconstituted in 10-12 μl of 0.1 % formic acid and analyzed on a LTQ Orbitrap Elite mass spectrometer (Thermo Fisher Scientific, Bremen, Germany) or a Fusion Lumos mass spectrometer (Thermo Fisher Scientific, San Jose, USA).

    Article Title: Phosphorylation Changes in Response to Kinase Inhibitor H89 in PKA-Null Cells
    Article Snippet: Total and phosphopeptides were analyzed using a Dionex UltiMate 3000 nano LC system connected to an Orbitrap Fusion Lumos ETD mass spectrometer equipped with an EASY-Spray ion source (Thermo Fisher Scientific). .. Total and phosphopeptides were analyzed using a Dionex UltiMate 3000 nano LC system connected to an Orbitrap Fusion Lumos ETD mass spectrometer equipped with an EASY-Spray ion source (Thermo Fisher Scientific).

    Injection:

    Article Title: Benchmarking common quantification strategies for large-scale phosphoproteomics
    Article Snippet: Nanoflow LC tandem MS All samples were analyzed on an Easy-nLC 1000 coupled to a Q-Exactive HF instrument (Thermo Fisher Scientific; TMT MS2 of Fig. ), an Orbitrap Fusion Lumos instrument (Thermo Fisher Scientific; Figs. , , TMT MS3 of 3, , Supplementary Fig. ), or a Q-Exactive HF-X instrument (Thermo Fisher Scientific; Fig. ), all equipped with a nanoelectrospray source. .. For SILAC and TMT in the technical comparison in Fig. , MS samples were diluted to contain a total peptide amount equal to one LFQ injection based on protein starting amount, to enable MS intensity-independent comparison of the method-inherent quantification characteristics.

    Article Title: Early urine proteome changes in the Walker-256 tail-vein injection rat model
    Article Snippet: Another twelve urine samples from three randomly selected Walker-256 tail-vein injection rats at four time points (day 2, 4, 6 and 9) and three urine samples from the corresponding control group were chosen for MS analysis. .. Peptides were analyzed with an Orbitrap Fusion Lumos Tribrid mass spectrometer (Thermo Fisher Scientific, Waltham, MA).

    Article Title: Enzyme reversal to explore the function of yeast E3 ubiquitin-ligases
    Article Snippet: × 10 cm using the Thermo EZ nLC 1200 and analyzed on an Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific, San Jose, CA). .. For each injection, an estimated 0.5 μg of total peptide was loaded and followed though a 120 minute linear gradient of 0% to 95% ACN in 0.1% Formic acid.

    Article Title: PepSAVI-MS reveals anticancer and antifungal cycloviolacins in Viola odorata
    Article Snippet: 1 µL of sample at a concentration of approximately 1 mM total protein content was injected on column per experiment. .. The chromatography system was coupled to Thermo Scientific Orbitrap Fusion Lumos mass spectrometer (San Jose, CA, USA) outfitted with a 193 nm excimer laser (Coherent Excistar 500 Hz, Santa Clara, CA, USA) to allow photodissociation, as implemented and described previously( ).

    Article Title: Targeted MultiNotch MS3 Approach for Relative Quantification of N-Glycans Using Multiplexed Carbonyl-Reactive Isobaric Tags
    Article Snippet: An Orbitrap Fusion Lumos Tribrid quadrupole-ion trap-Orbitrap mass spectrometer with NanoSpray Flex ion source (Thermo Scientific, Bremen, Germany) was used for data acquisition. .. Full MS was acquired with an m / z range of 500–1700 and detected in Orbitrap at resolution of 120 K at m / z 200 with automatic gain control (AGC) target of 1 × 106 , maximal injection time of 100 ms, and 1 microscan.

    Article Title: Improved Method for Determining Absolute Phosphorylation Stoichiometry Using Bayesian Statistics and Isobaric Labeling
    Article Snippet: Data for all LC–MS/MS experiments were collected on an Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific, San Jose, CA) with LC separation performed on an attached Proxeon EASY-nLC 1200 liquid chromatography (LC) pump (Thermo Fisher Scientific). .. A 150 min gradient of 6% to 25% acetonitrile in 0.125% formic acid was used at a flow rate of ~450 nL/min to separate peptides from the pooled TMT-labeled samples: MS1 spectra (Orbitrap resolution, 120 000; mass range, 350–1400 m/z ; automatic gain control (AGC) target, 5 × 105 ; maximum injection time, 100 ms).

    Article Title: Early urine proteome changes in the Walker-256 tail-vein injection rat model
    Article Snippet: Parallel reaction monitoring (PRM) mass spectrometry and data analysis Sixteen urine samples from another four randomly selected Walker-256 tail-vein injection rats at four time points (day 2, 4, 6 and 9) and 4 urine samples from the corresponding control group were chosen for PRM analysis. .. PRM samples were analyzed by the EASY-nLC 1200 HPLC system (Thermo Fisher Scientific, Waltham, MA) coupled to the Orbitrap Fusion Lumos Tribrid mass spectrometer (Thermo Fisher Scientific, Waltham, MA).

    Chick Chorioallantoic Membrane Assay:

    Article Title: Leishmania major degrades murine CXCL1 – An immune evasion strategy
    Article Snippet: The gel pieces were washed with 25mM ammonium bicarbonate: acetonitrile (1:1, v/v) twice to remove excess DTT and CAM. .. The combined extracts were concentrated in a lyophilizer and rehydrated with 15μl of mobile phase A. LC-MS/MS: Mass spectrometry data were collected using an Orbitrap Fusion Lumos mass spectrometer (Thermofisher) coupled to an Easy-nLC-120 System (Proxeon P/N LC1400).

    SDS Page:

    Article Title: Leishmania major degrades murine CXCL1 – An immune evasion strategy
    Article Snippet: Briefly, the targeted protein bands from SDS-PAGE gel were manually excised, cut into 1mm3 pieces, and washed in 100mM ammonium bicarbonate:acetonitrile (1:1, v/v) and 25mM ammonium bicarbonate /acetonitrile (1:1, v/v), respectively, to achieve complete de-staining. .. The combined extracts were concentrated in a lyophilizer and rehydrated with 15μl of mobile phase A. LC-MS/MS: Mass spectrometry data were collected using an Orbitrap Fusion Lumos mass spectrometer (Thermofisher) coupled to an Easy-nLC-120 System (Proxeon P/N LC1400).

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  • 90
    Thermo Fisher orbitrap fusion lumos mass spectrometer
    Gas phase segmentation (GPS) improves ADPr peptide detection. (A) A screenshot of the ADP-ribose product ion triggered EThcD and HCD data acquisition method on the <t>Orbitrap</t> Fusion <t>Lumos.</t> (B) Area under the curve (AUC) of the four ADPr fragment ions (136.06, 250.09, 348.07, and 428.04 m / z ) dissociated from an ARTD8/PARP14-MARylated STAT1 peptide using HCD. (C) Pilot Af1521 enrichment study to determine the optimal collision energy for ADP-ribose product ion screening and ADPr peptide identification. Only high confidence (HCD and EThcD combined peptides) were used in this plot (more details in Figure 3 ). (D) A schematic showing the principle of GPS using multiple injections. (E) The extracted ion chromatograms of the ADPr fragment peak (348.07 m / z ) in each full scan and GPS injection. (F,G) Precursor ion m / z and retention time of triggered EThcD spectra in full scan and combined GPS scans.
    Orbitrap Fusion Lumos Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 82 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Gas phase segmentation (GPS) improves ADPr peptide detection. (A) A screenshot of the ADP-ribose product ion triggered EThcD and HCD data acquisition method on the Orbitrap Fusion Lumos. (B) Area under the curve (AUC) of the four ADPr fragment ions (136.06, 250.09, 348.07, and 428.04 m / z ) dissociated from an ARTD8/PARP14-MARylated STAT1 peptide using HCD. (C) Pilot Af1521 enrichment study to determine the optimal collision energy for ADP-ribose product ion screening and ADPr peptide identification. Only high confidence (HCD and EThcD combined peptides) were used in this plot (more details in Figure 3 ). (D) A schematic showing the principle of GPS using multiple injections. (E) The extracted ion chromatograms of the ADPr fragment peak (348.07 m / z ) in each full scan and GPS injection. (F,G) Precursor ion m / z and retention time of triggered EThcD spectra in full scan and combined GPS scans.

    Journal: Journal of Proteome Research

    Article Title: A Study into the ADP-Ribosylome of IFN-γ-Stimulated THP-1 Human Macrophage-like Cells Identifies ARTD8/PARP14 and ARTD9/PARP9 ADP-Ribosylation

    doi: 10.1021/acs.jproteome.8b00895

    Figure Lengend Snippet: Gas phase segmentation (GPS) improves ADPr peptide detection. (A) A screenshot of the ADP-ribose product ion triggered EThcD and HCD data acquisition method on the Orbitrap Fusion Lumos. (B) Area under the curve (AUC) of the four ADPr fragment ions (136.06, 250.09, 348.07, and 428.04 m / z ) dissociated from an ARTD8/PARP14-MARylated STAT1 peptide using HCD. (C) Pilot Af1521 enrichment study to determine the optimal collision energy for ADP-ribose product ion screening and ADPr peptide identification. Only high confidence (HCD and EThcD combined peptides) were used in this plot (more details in Figure 3 ). (D) A schematic showing the principle of GPS using multiple injections. (E) The extracted ion chromatograms of the ADPr fragment peak (348.07 m / z ) in each full scan and GPS injection. (F,G) Precursor ion m / z and retention time of triggered EThcD spectra in full scan and combined GPS scans.

    Article Snippet: LC–MS/MS Analysis All peptide samples were analyzed on an Orbitrap Fusion Lumos mass spectrometer fronted with an EASY-Spray Source, coupled to an Easy-nLC1000 HPLC pump (Thermo Fisher Scientific).

    Techniques: Injection

    DiLeu cPILOT experimental workflow. Protein digest samples undergo stable isotope dimethyl labeling of peptide N-termini with either light [–(CH 3 ) 2 ] or heavy [–( 13 C 2 H 3 ) 2 ] dimethyl groups at low pH followed by labeling of lysine residues with 12-plex DiLeu isobaric tags at high pH. Pooled samples are analyzed by LC-MS/MS on the Orbitrap Fusion Lumos using CID MS 2 acquisition of peak pairs for peptide sequence identification and HCD SPS-MS 3 acquisition for accurate 24-plex quantification via DiLeu reporter ions.

    Journal: Analytical chemistry

    Article Title: Increased N,N-Dimethyl Leucine Isobaric Tag Multiplexing by a Combined Precursor Isotopic Labeling and Isobaric Tagging Approach

    doi: 10.1021/acs.analchem.8b01301

    Figure Lengend Snippet: DiLeu cPILOT experimental workflow. Protein digest samples undergo stable isotope dimethyl labeling of peptide N-termini with either light [–(CH 3 ) 2 ] or heavy [–( 13 C 2 H 3 ) 2 ] dimethyl groups at low pH followed by labeling of lysine residues with 12-plex DiLeu isobaric tags at high pH. Pooled samples are analyzed by LC-MS/MS on the Orbitrap Fusion Lumos using CID MS 2 acquisition of peak pairs for peptide sequence identification and HCD SPS-MS 3 acquisition for accurate 24-plex quantification via DiLeu reporter ions.

    Article Snippet: Samples were analyzed by nanoLC-MS/MS using a Dionex UltiMate 3000 UPLC system coupled to a Thermo Scientific Orbitrap Fusion Lumos mass spectrometer.

    Techniques: Labeling, Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry, Sequencing

    Cluster analysis of validated seven differential proteins by different mass spectrometers. Cluster analysis of differential proteins by Triple TOF 5600 ( a ) and by Orbitrap Fusion Lumos ( b ).

    Journal: Scientific Reports

    Article Title: Early urine proteome changes in the Walker-256 tail-vein injection rat model

    doi: 10.1038/s41598-019-50301-1

    Figure Lengend Snippet: Cluster analysis of validated seven differential proteins by different mass spectrometers. Cluster analysis of differential proteins by Triple TOF 5600 ( a ) and by Orbitrap Fusion Lumos ( b ).

    Article Snippet: PRM samples were analyzed by the EASY-nLC 1200 HPLC system (Thermo Fisher Scientific, Waltham, MA) coupled to the Orbitrap Fusion Lumos Tribrid mass spectrometer (Thermo Fisher Scientific, Waltham, MA).

    Techniques:

    Experimental overview of the SPS-MS3 strategy Two cell types, α7KO-HAP1 and HAP1wt, were propagated and designated cultures were mock or nicotine-treated. Proteins were extracted and then digested with LysC and trypsin. The resulting peptides were labeled with TMT, pooled, and fractionated via basic pH reversed-phase (BPRP) high performance liquid chromatography (HPLC) prior to SPS-MS3 analysis on an Orbitrap Fusion Lumos mass spectrometer.

    Journal: Proteomics

    Article Title: Isobaric tag-based protein profiling of a nicotine-treated alpha7 nicotinic receptor-null human haploid cell line

    doi: 10.1002/pmic.201700475

    Figure Lengend Snippet: Experimental overview of the SPS-MS3 strategy Two cell types, α7KO-HAP1 and HAP1wt, were propagated and designated cultures were mock or nicotine-treated. Proteins were extracted and then digested with LysC and trypsin. The resulting peptides were labeled with TMT, pooled, and fractionated via basic pH reversed-phase (BPRP) high performance liquid chromatography (HPLC) prior to SPS-MS3 analysis on an Orbitrap Fusion Lumos mass spectrometer.

    Article Snippet: All samples were analyzed on an Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific, San Jose, CA) coupled to a Proxeon EASY-nLC 1200 liquid chromatography (LC) pump (Thermo Fisher Scientific).

    Techniques: Labeling, High Performance Liquid Chromatography, Mass Spectrometry